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The map label for this gene is mltB [H]

Identifier: 218930007

GI number: 218930007

Start: 3304260

End: 3305372

Strand: Reverse

Name: mltB [H]

Synonym: YPO2957

Alternate gene names: 218930007

Gene position: 3305372-3304260 (Counterclockwise)

Preceding gene: 218930011

Following gene: 218930006

Centisome position: 71.03

GC content: 51.48

Gene sequence:

>1113_bases
ATGGTTTATAGGTATTCGATGCGTTATTTGGCTGTTATTCTCCCTTTGTTGACGTTGCTCTCAGCTTGTAGTAGCCAGCC
GACACCTCCTGTTGCTGCAACCCCGTCAGATAGCCCTGTTCAGGGCGGTTTTTTGCTTGAGCCGGAACATACGGGCCAGT
TGCAAAACGGTGATTTTGCCTTTAACCCAGATACCACGCGTTTTGTTGACAAAATGGTGCGTGAGCACGGTTTTGATCGC
CAGCAACTGCACGATGTATTGGCTCAGACCCAGCGGCTGGACTGGGTTATTCGTTTGATGGATAAGCAAGCGCCGACGTC
CCGACCACCTTCTGAGCCAAACGGTGCCTGGAATCGCTATCGCAATAAATTTATTACTCCTGATAATGTGCAGAACGGTG
TGGTTTTCTGGAACCAATATGAAGGTGCGCTGCAACGTGCCTGGGAGGTGTACGGTGTGCCGCCGGAAATTATCGTCGGT
ATCATCGGGGTTGAAACGCGTTGGGGCCGGGTGATGGGGAAAACCCGAATCATCGACGCCTTGGCGACGCTATCCTTTGC
TTACCCACGGCGTGCAACATTCTTTACGGGTGAGTTGGAAACCTTCTTATTAATGGCGCGTGCTGAGGGGAAAGACCCAC
TCAGTCTACGGGGCTCCTATGCGGGGGCAATGGGCTATGGTCAATTTATGCCATCGTCATTTAAGCGCTATGCAGTGGAT
TTTGATGGCAACGGCCACATCAACTTATGGGATCCCGTTGATGCTATCGGCAGTGTTGCCAATTACTTCAAATCCCACGG
TTGGACGAAAGGTGCGCCGGTAGCTGTACCTGCCAACGGACAGGCTCCAAATCTCGAAAATGGATTTAAGACCCGTTATC
CAATCTCAACTTTAGCGGCAGCAGGGCTTAGCCCCAAAGGGTCGTTAGGCGATTATCAGGAGGCCAGTTTGCTGCGCTTT
GATGTGGGTACCGGTTATCAATATTGGTACGGTTTGCCCAACTTCTACACGATTACCCGCTATAACCACAGTACCTATTA
TGCGATGGCAGTATGGCAGTTAGGGGAAGCCGTGGGCAGGGCGCGCAAAGGGGAGTCAATGATTACCCAATAG

Upstream 100 bases:

>100_bases
GTCATATTTAGCTTTCATTATATGAAGAGTACCGGCTTTAACGGCATTTTCCTGTTTGTTCACAACAGACAATGGCTTAC
CATAGCTATCACTTATTTGT

Downstream 100 bases:

>100_bases
CATGAAAAAGAGGGCTGCATTAGGGTTATGCCGCCCTCTTTTCTTAACGATATTTACTCATCTATTAGATGTGAAATATC
ATCAGGATGCACTGAGGGTG

Product: murein hydrolase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MVYRYSMRYLAVILPLLTLLSACSSQPTPPVAATPSDSPVQGGFLLEPEHTGQLQNGDFAFNPDTTRFVDKMVREHGFDR
QQLHDVLAQTQRLDWVIRLMDKQAPTSRPPSEPNGAWNRYRNKFITPDNVQNGVVFWNQYEGALQRAWEVYGVPPEIIVG
IIGVETRWGRVMGKTRIIDALATLSFAYPRRATFFTGELETFLLMARAEGKDPLSLRGSYAGAMGYGQFMPSSFKRYAVD
FDGNGHINLWDPVDAIGSVANYFKSHGWTKGAPVAVPANGQAPNLENGFKTRYPISTLAAAGLSPKGSLGDYQEASLLRF
DVGTGYQYWYGLPNFYTITRYNHSTYYAMAVWQLGEAVGRARKGESMITQ

Sequences:

>Translated_370_residues
MVYRYSMRYLAVILPLLTLLSACSSQPTPPVAATPSDSPVQGGFLLEPEHTGQLQNGDFAFNPDTTRFVDKMVREHGFDR
QQLHDVLAQTQRLDWVIRLMDKQAPTSRPPSEPNGAWNRYRNKFITPDNVQNGVVFWNQYEGALQRAWEVYGVPPEIIVG
IIGVETRWGRVMGKTRIIDALATLSFAYPRRATFFTGELETFLLMARAEGKDPLSLRGSYAGAMGYGQFMPSSFKRYAVD
FDGNGHINLWDPVDAIGSVANYFKSHGWTKGAPVAVPANGQAPNLENGFKTRYPISTLAAAGLSPKGSLGDYQEASLLRF
DVGTGYQYWYGLPNFYTITRYNHSTYYAMAVWQLGEAVGRARKGESMITQ
>Mature_370_residues
MVYRYSMRYLAVILPLLTLLSACSSQPTPPVAATPSDSPVQGGFLLEPEHTGQLQNGDFAFNPDTTRFVDKMVREHGFDR
QQLHDVLAQTQRLDWVIRLMDKQAPTSRPPSEPNGAWNRYRNKFITPDNVQNGVVFWNQYEGALQRAWEVYGVPPEIIVG
IIGVETRWGRVMGKTRIIDALATLSFAYPRRATFFTGELETFLLMARAEGKDPLSLRGSYAGAMGYGQFMPSSFKRYAVD
FDGNGHINLWDPVDAIGSVANYFKSHGWTKGAPVAVPANGQAPNLENGFKTRYPISTLAAAGLSPKGSLGDYQEASLLRF
DVGTGYQYWYGLPNFYTITRYNHSTYYAMAVWQLGEAVGRARKGESMITQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=360, Percent_Identity=74.1666666666667, Blast_Score=552, Evalue=1e-158,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 41311; Mature: 41311

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYRYSMRYLAVILPLLTLLSACSSQPTPPVAATPSDSPVQGGFLLEPEHTGQLQNGDFA
CEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCEEECCCCCCCCCCCCEE
FNPDTTRFVDKMVREHGFDRQQLHDVLAQTQRLDWVIRLMDKQAPTSRPPSEPNGAWNRY
ECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
RNKFITPDNVQNGVVFWNQYEGALQRAWEVYGVPPEIIVGIIGVETRWGRVMGKTRIIDA
HCCCCCCCCCCCCEEEEECHHHHHHHHHHHCCCCHHHEEEEECCCCHHHHHHHHHHHHHH
LATLSFAYPRRATFFTGELETFLLMARAEGKDPLSLRGSYAGAMGYGQFMPSSFKRYAVD
HHHHHHCCCCCCEEEECCHHHHHEEHHCCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEE
FDGNGHINLWDPVDAIGSVANYFKSHGWTKGAPVAVPANGQAPNLENGFKTRYPISTLAA
ECCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHH
AGLSPKGSLGDYQEASLLRFDVGTGYQYWYGLPNFYTITRYNHSTYYAMAVWQLGEAVGR
CCCCCCCCCCCCCCCEEEEEECCCCCCEEECCCCEEEEEEECCCEEEEHHHHHHHHHHHH
ARKGESMITQ
HHCCCHHCCC
>Mature Secondary Structure
MVYRYSMRYLAVILPLLTLLSACSSQPTPPVAATPSDSPVQGGFLLEPEHTGQLQNGDFA
CEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCEEECCCCCCCCCCCCEE
FNPDTTRFVDKMVREHGFDRQQLHDVLAQTQRLDWVIRLMDKQAPTSRPPSEPNGAWNRY
ECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
RNKFITPDNVQNGVVFWNQYEGALQRAWEVYGVPPEIIVGIIGVETRWGRVMGKTRIIDA
HCCCCCCCCCCCCEEEEECHHHHHHHHHHHCCCCHHHEEEEECCCCHHHHHHHHHHHHHH
LATLSFAYPRRATFFTGELETFLLMARAEGKDPLSLRGSYAGAMGYGQFMPSSFKRYAVD
HHHHHHCCCCCCEEEECCHHHHHEEHHCCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEE
FDGNGHINLWDPVDAIGSVANYFKSHGWTKGAPVAVPANGQAPNLENGFKTRYPISTLAA
ECCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHH
AGLSPKGSLGDYQEASLLRFDVGTGYQYWYGLPNFYTITRYNHSTYYAMAVWQLGEAVGR
CCCCCCCCCCCCCCCEEEEEECCCCCCEEECCCCEEEEEEECCCEEEEHHHHHHHHHHHH
ARKGESMITQ
HHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]