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The map label for this gene is emtA [H]

Identifier: 218929527

GI number: 218929527

Start: 2737868

End: 2738530

Strand: Reverse

Name: emtA [H]

Synonym: YPO2438

Alternate gene names: 218929527

Gene position: 2738530-2737868 (Counterclockwise)

Preceding gene: 218929534

Following gene: 218929525

Centisome position: 58.85

GC content: 47.06

Gene sequence:

>663_bases
GTGAATTCAAAAGTCGGTTGCCTAATGGCAATTTTATTGTTGGCGGGGTGTGCTAAACAAACCCCACCACCACAAGCGAA
TAACGGGTGGCTGAAAAAAACGCCACAGGGCAATTCTCTTAACGTAGCGAAAAGCAGTGCCGGTTCAACTACCGTTGCTT
ATAGCGACGTCATTAAACAAGCCGCGAGCCATTACGGCGTTGATGAAACACTGATTAAAGCGATTATCCAGGTGGAATCT
GGTTACAACCCGGATGTTGTGAGTAGATCCAATGCCGTAGGCTTGATGCAGATTAAAGCCTCTACCGCCGGGCGTGATGC
GTACCGGATGAAAGGGCGAAATGGTCAACCGAGCTCTCGTGAGCTAAAAGATCCAGTGAAGAATATTGATATTGGTGCTG
CTTATATCAACATTCTACAAAATCAGCAGTTAGCGGGGATCAACGACCCGCAGACATTACGTTATGCCACCATCGTTTCT
TATGCGAATGGCGCGGGGGCTATGTTGCGGACTTTTTCATCAGATAAACGTCTGGCAGTCAATAAAATTAATAGTCTTAG
CCCAAACGAATTTTATCAGCATATACAAAAGAAGCACCCGGCTGCACAGGCTCCACGTTACTTGTGGAAGGTGGATACCG
CTTACCGGGCGATGTCAGAGTAA

Upstream 100 bases:

>100_bases
ATCATTATGTTGAATATGGACAAACTAGGCCAAAAAATCTATTTGGGCGGAGTGACAACTTGTATATGCTACAAAACGGC
TTATCAGCACGGGGAATACT

Downstream 100 bases:

>100_bases
TGCTAAAACGCTAACCGATGTAGCCTATGGATGGTGTAGCATCGGCTAGCGTTTACTTTAAATTTTACGGCAAGTTACTT
TTTTATTTTACTTTGAAATT

Product: putative membrane-bound lytic murein transglycosylase

Products: NA

Alternate protein names: Peptidoglycan lytic endotransglycosylase [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MNSKVGCLMAILLLAGCAKQTPPPQANNGWLKKTPQGNSLNVAKSSAGSTTVAYSDVIKQAASHYGVDETLIKAIIQVES
GYNPDVVSRSNAVGLMQIKASTAGRDAYRMKGRNGQPSSRELKDPVKNIDIGAAYINILQNQQLAGINDPQTLRYATIVS
YANGAGAMLRTFSSDKRLAVNKINSLSPNEFYQHIQKKHPAAQAPRYLWKVDTAYRAMSE

Sequences:

>Translated_220_residues
MNSKVGCLMAILLLAGCAKQTPPPQANNGWLKKTPQGNSLNVAKSSAGSTTVAYSDVIKQAASHYGVDETLIKAIIQVES
GYNPDVVSRSNAVGLMQIKASTAGRDAYRMKGRNGQPSSRELKDPVKNIDIGAAYINILQNQQLAGINDPQTLRYATIVS
YANGAGAMLRTFSSDKRLAVNKINSLSPNEFYQHIQKKHPAAQAPRYLWKVDTAYRAMSE
>Mature_220_residues
MNSKVGCLMAILLLAGCAKQTPPPQANNGWLKKTPQGNSLNVAKSSAGSTTVAYSDVIKQAASHYGVDETLIKAIIQVES
GYNPDVVSRSNAVGLMQIKASTAGRDAYRMKGRNGQPSSRELKDPVKNIDIGAAYINILQNQQLAGINDPQTLRYATIVS
YANGAGAMLRTFSSDKRLAVNKINSLSPNEFYQHIQKKHPAAQAPRYLWKVDTAYRAMSE

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division. Preferentially cleaves at a distance of more than two disaccharide units from the ends of the glycan chain [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87081855, Length=211, Percent_Identity=49.7630331753554, Blast_Score=209, Evalue=8e-56,
Organism=Escherichia coli, GI87082191, Length=164, Percent_Identity=49.390243902439, Blast_Score=173, Evalue=7e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 23827; Mature: 23827

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKVGCLMAILLLAGCAKQTPPPQANNGWLKKTPQGNSLNVAKSSAGSTTVAYSDVIKQ
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCHHHHHHHHHH
AASHYGVDETLIKAIIQVESGYNPDVVSRSNAVGLMQIKASTAGRDAYRMKGRNGQPSSR
HHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCHHHHCCCCCCCCCCH
ELKDPVKNIDIGAAYINILQNQQLAGINDPQTLRYATIVSYANGAGAMLRTFSSDKRLAV
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCHH
NKINSLSPNEFYQHIQKKHPAAQAPRYLWKVDTAYRAMSE
HHHCCCCHHHHHHHHHHHCCHHHCCHHEEHHHHHHHHHCC
>Mature Secondary Structure
MNSKVGCLMAILLLAGCAKQTPPPQANNGWLKKTPQGNSLNVAKSSAGSTTVAYSDVIKQ
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCHHHHHHHHHH
AASHYGVDETLIKAIIQVESGYNPDVVSRSNAVGLMQIKASTAGRDAYRMKGRNGQPSSR
HHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCHHHHCCCCCCCCCCH
ELKDPVKNIDIGAAYINILQNQQLAGINDPQTLRYATIVSYANGAGAMLRTFSSDKRLAV
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCHH
NKINSLSPNEFYQHIQKKHPAAQAPRYLWKVDTAYRAMSE
HHHCCCCHHHHHHHHHHHCCHHHCCHHEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA