Click here to switch to the map view.

The map label for this gene is pmrF [H]

Identifier: 218929509

GI number: 218929509

Start: 2721869

End: 2722852

Strand: Reverse

Name: pmrF [H]

Synonym: YPO2421

Alternate gene names: 218929509

Gene position: 2722852-2721869 (Counterclockwise)

Preceding gene: 218929510

Following gene: 218929508

Centisome position: 58.51

GC content: 46.34

Gene sequence:

>984_bases
GTGTCACTAAATGAACCAATTAAGAAGGTCTCCATTGTTATTCCTGTCTATAACGAGCAAGAGAGCTTGCCAGCATTAAT
AGACAGAACGACGGCTGCCTGTAAATTACTTACACAAGCTTATGAAATAATTTTGGTTGATGACGGTAGCAGCGACAATT
CTACTGAGCTTTTGACTGCTGCGGCAAATGATCCTGATAGCCATATAATTGCGATTTTGCTAAACCGTAATTATGGTCAA
CATTCCGCCATTATGGCGGGATTTAATCAGGTTAGCGGTGATTTAATTATTACACTTGATGCTGACTTACAAAATCCGCC
TGAAGAAACTCCACGTTTAGTTCATGTAGCAGAAGAGGGCTATGACGTGGTTGGTACCGTGCGTGCTAATCGCCAAGACT
CCCTGTTCCGTAAAACCGCATCGCGTATGATCAATATGATGATCCAACGTGCTACAGGGAAATCAATGGGTGACTACGGC
TGCATGCTACGAGCTTATCGACGTCACATTGTTGAAGCAATGTTACACTGTCACGAGCGCAGTACGTTTATCCCAATCCT
CGCGAATACCTTCGCCCGGCGGACGACAGAGATTACGGTCCATCACGCCGAGCGTGAATTTGGCAACTCCAAATATAGCC
TGATGAGGCTTATCAATCTGATGTATGACCTGATCACCTGCCTGACAACGACGCCGTTACGTTTGTTAAGTCTGGTGGGG
AGTGCCATTGCTCTTTTGGGCTTCACTTTCTCAGTGCTGCTGGTGGCCTTACGCTTGATCTTTGGTCCTGAGTGGGCTGG
CGGCGGCGTTTTTACACTCTTCGCCGTATTGTTCATGTTCATCGGTGCTCAGTTTGTTGGTATGGGGTTACTTGGTGAAT
ATATCGGTCGTATTTATAACGATGTGCGCGCTCGCCCACGTTACTTTGTTCAGAAAGTGGTCGGCGCGGAGCAGACCGAA
AATAATCAGGATGTAGAAAAATGA

Upstream 100 bases:

>100_bases
AACTCAGCCAGATTGTGCACGTTACCGCTTTTCCCCGATATGCTAGACAGTGATATTGAACGAGTGGCTAATGCATTAAC
AACAATTATAGGGTCACACC

Downstream 100 bases:

>100_bases
AAGCGATTGTATTTGCCTATCATGATATTGGTTGCGTGGGGCTAAACGCATTAGCAGAGGCGGGTTACGATATTCAGGCG
GTATTTACCCATACTGATAA

Product: undecaprenyl phosphate 4-deoxy-4-formamido-L-arabinose transferase

Products: NA

Alternate protein names: Undecaprenyl-phosphate Ara4FN transferase; Ara4FN transferase [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSLNEPIKKVSIVIPVYNEQESLPALIDRTTAACKLLTQAYEIILVDDGSSDNSTELLTAAANDPDSHIIAILLNRNYGQ
HSAIMAGFNQVSGDLIITLDADLQNPPEETPRLVHVAEEGYDVVGTVRANRQDSLFRKTASRMINMMIQRATGKSMGDYG
CMLRAYRRHIVEAMLHCHERSTFIPILANTFARRTTEITVHHAEREFGNSKYSLMRLINLMYDLITCLTTTPLRLLSLVG
SAIALLGFTFSVLLVALRLIFGPEWAGGGVFTLFAVLFMFIGAQFVGMGLLGEYIGRIYNDVRARPRYFVQKVVGAEQTE
NNQDVEK

Sequences:

>Translated_327_residues
MSLNEPIKKVSIVIPVYNEQESLPALIDRTTAACKLLTQAYEIILVDDGSSDNSTELLTAAANDPDSHIIAILLNRNYGQ
HSAIMAGFNQVSGDLIITLDADLQNPPEETPRLVHVAEEGYDVVGTVRANRQDSLFRKTASRMINMMIQRATGKSMGDYG
CMLRAYRRHIVEAMLHCHERSTFIPILANTFARRTTEITVHHAEREFGNSKYSLMRLINLMYDLITCLTTTPLRLLSLVG
SAIALLGFTFSVLLVALRLIFGPEWAGGGVFTLFAVLFMFIGAQFVGMGLLGEYIGRIYNDVRARPRYFVQKVVGAEQTE
NNQDVEK
>Mature_326_residues
SLNEPIKKVSIVIPVYNEQESLPALIDRTTAACKLLTQAYEIILVDDGSSDNSTELLTAAANDPDSHIIAILLNRNYGQH
SAIMAGFNQVSGDLIITLDADLQNPPEETPRLVHVAEEGYDVVGTVRANRQDSLFRKTASRMINMMIQRATGKSMGDYGC
MLRAYRRHIVEAMLHCHERSTFIPILANTFARRTTEITVHHAEREFGNSKYSLMRLINLMYDLITCLTTTPLRLLSLVGS
AIALLGFTFSVLLVALRLIFGPEWAGGGVFTLFAVLFMFIGAQFVGMGLLGEYIGRIYNDVRARPRYFVQKVVGAEQTEN
NQDVEK

Specific function: Catalyzes the transfer of 4-deoxy-4-formamido-L- arabinose from UDP to undecaprenyl phosphate. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides [H]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Homo sapiens, GI4503363, Length=235, Percent_Identity=25.531914893617, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1788588, Length=317, Percent_Identity=77.602523659306, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI1788692, Length=311, Percent_Identity=31.5112540192926, Blast_Score=169, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6325441, Length=148, Percent_Identity=29.7297297297297, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585265, Length=239, Percent_Identity=26.3598326359833, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022857
- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: =2.7.8.30 [H]

Molecular weight: Translated: 36422; Mature: 36290

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNEPIKKVSIVIPVYNEQESLPALIDRTTAACKLLTQAYEIILVDDGSSDNSTELLTA
CCCCCCCHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEE
AANDPDSHIIAILLNRNYGQHSAIMAGFNQVSGDLIITLDADLQNPPEETPRLVHVAEEG
ECCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEECCCC
YDVVGTVRANRQDSLFRKTASRMINMMIQRATGKSMGDYGCMLRAYRRHIVEAMLHCHER
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
STFIPILANTFARRTTEITVHHAEREFGNSKYSLMRLINLMYDLITCLTTTPLRLLSLVG
CCCHHHHHHHHHHHHHEEEEEECHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAIALLGFTFSVLLVALRLIFGPEWAGGGVFTLFAVLFMFIGAQFVGMGLLGEYIGRIYN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVRARPRYFVQKVVGAEQTENNQDVEK
HHHCCHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SLNEPIKKVSIVIPVYNEQESLPALIDRTTAACKLLTQAYEIILVDDGSSDNSTELLTA
CCCCCCHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEE
AANDPDSHIIAILLNRNYGQHSAIMAGFNQVSGDLIITLDADLQNPPEETPRLVHVAEEG
ECCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEECCCC
YDVVGTVRANRQDSLFRKTASRMINMMIQRATGKSMGDYGCMLRAYRRHIVEAMLHCHER
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
STFIPILANTFARRTTEITVHHAEREFGNSKYSLMRLINLMYDLITCLTTTPLRLLSLVG
CCCHHHHHHHHHHHHHEEEEEECHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAIALLGFTFSVLLVALRLIFGPEWAGGGVFTLFAVLFMFIGAQFVGMGLLGEYIGRIYN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVRARPRYFVQKVVGAEQTENNQDVEK
HHHCCHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA