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The map label for this gene is gloA [H]

Identifier: 218929472

GI number: 218929472

Start: 2676436

End: 2676843

Strand: Direct

Name: gloA [H]

Synonym: YPO2381

Alternate gene names: 218929472

Gene position: 2676436-2676843 (Clockwise)

Preceding gene: 218929470

Following gene: 218929473

Centisome position: 57.51

GC content: 48.53

Gene sequence:

>408_bases
ATGCGCTTACTCCATACCATGCTCCGCGTCGGTGACCTGCAACGTTCTATCGATTTCTACACCAAGGTATTAGGGATGCG
TTTACTGCGTACCAGCGAAAATACTGAATATAAATACTCGTTGGCATTCGTAGGCTATAGCGATGAAAGTAAAGGTTCGG
TGATTGAACTGACGTATAACTGGGGCGTTGACCAGTACGATATGGGCACCGCATTCGGCCATCTGGCTCTGGGTGTTGAT
GATGTCGCCGCAACGTGTGATCAAATTCGCCAGGCAGGCGGTAAAGTCACCCGCGAAGCTGGCCCGGTAAAAGGCGGTAA
TACCATTATTGCTTTTGTTGAAGATCCAGATGGCTACAAAATTGAGTTAATTGAGAATAAGAGCGCGGGTGACTGCCTCG
GAAACTGA

Upstream 100 bases:

>100_bases
CTGTAACCCATTGAACTAATTTAATTACCGGGGCTATACTTGAACCTAATTTTATTCACTCTTGCCAGAGCGTCGGGCAA
AATGTAAACAGAGGGAAATT

Downstream 100 bases:

>100_bases
TTAACTTAAGGGCACCTCGGACGGTGCCTTTTACTTATCACAATTAACCCCCTGCATCCCTCGCGAAAATTTGACATAAT
GCACGCTGAATTCGATAAAG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MRLLHTMLRVGDLQRSIDFYTKVLGMRLLRTSENTEYKYSLAFVGYSDESKGSVIELTYNWGVDQYDMGTAFGHLALGVD
DVAATCDQIRQAGGKVTREAGPVKGGNTIIAFVEDPDGYKIELIENKSAGDCLGN

Sequences:

>Translated_135_residues
MRLLHTMLRVGDLQRSIDFYTKVLGMRLLRTSENTEYKYSLAFVGYSDESKGSVIELTYNWGVDQYDMGTAFGHLALGVD
DVAATCDQIRQAGGKVTREAGPVKGGNTIIAFVEDPDGYKIELIENKSAGDCLGN
>Mature_135_residues
MRLLHTMLRVGDLQRSIDFYTKVLGMRLLRTSENTEYKYSLAFVGYSDESKGSVIELTYNWGVDQYDMGTAFGHLALGVD
DVAATCDQIRQAGGKVTREAGPVKGGNTIIAFVEDPDGYKIELIENKSAGDCLGN

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=152, Percent_Identity=36.1842105263158, Blast_Score=93, Evalue=7e-20,
Organism=Escherichia coli, GI1787940, Length=135, Percent_Identity=79.2592592592593, Blast_Score=227, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6323639, Length=141, Percent_Identity=39.0070921985816, Blast_Score=99, Evalue=2e-22,
Organism=Drosophila melanogaster, GI20129743, Length=148, Percent_Identity=35.1351351351351, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19920404, Length=139, Percent_Identity=33.0935251798561, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14834; Mature: 14834

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLHTMLRVGDLQRSIDFYTKVLGMRLLRTSENTEYKYSLAFVGYSDESKGSVIELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCEEEEEEC
WGVDQYDMGTAFGHLALGVDDVAATCDQIRQAGGKVTREAGPVKGGNTIIAFVEDPDGYK
CCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEEECCCCCE
IELIENKSAGDCLGN
EEEEECCCCCCCCCC
>Mature Secondary Structure
MRLLHTMLRVGDLQRSIDFYTKVLGMRLLRTSENTEYKYSLAFVGYSDESKGSVIELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCEEEEEEC
WGVDQYDMGTAFGHLALGVDDVAATCDQIRQAGGKVTREAGPVKGGNTIIAFVEDPDGYK
CCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEEECCCCCE
IELIENKSAGDCLGN
EEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]