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The map label for this gene is rstB [H]

Identifier: 218929401

GI number: 218929401

Start: 2596225

End: 2597502

Strand: Direct

Name: rstB [H]

Synonym: YPO2309

Alternate gene names: 218929401

Gene position: 2596225-2597502 (Clockwise)

Preceding gene: 218929400

Following gene: 218929402

Centisome position: 55.79

GC content: 47.5

Gene sequence:

>1278_bases
TTGTTGTTATTCGTTTGCTTCATGGTGATGGCGATGCTGGTTGGCCTGGTTTATAAGGTCACCGCTGAGCGCGCGGGTCG
TCAGTCTTTAGATGACCTGATGAAAAGTTCACTATCACTGATGCGCAGCGAATTGCGGGAGATCCCGCTAAAAGACTGGA
ATAAAACCATCGCAACATTAGATTTAAATCTTTCTTTTCAACTTCATATCGAACCATTAGATAAACGTGATCTTGGCGCA
CAATTGAACAAACGGCTACGTGCGGGTGAAATTATTGCATTGGATGATGAATATACCTTCCTGCAACGTATCCCTCGTAG
CCACTATGTTCTGGCGGTTGGCCCGGTCCCTTATCTATTTTATTTACACCAGATGAGGCTGCTCGATTTAGCATTATTAA
TCTTGATTGGCCTGTCGTTAGCTCTTCCGGTATTTCTCTGGATGAGACCGCACTGGAAAGATCTTCTCAAATTAGAAAAT
GCCGCTCAGAGACTGGGCTCAGGCCACCTTGACGAGCGGACTCATTTCGACCCCACTTCCAGTCTAAGCCGTTTAGGCGT
GGCTTTTAACCAAATGGCTGACAACATCAACACCCTGATTGTTAGCAAAAAATTACTGATTGATGGTATAGCACACGAAC
TACGGACACCATTGGTACGCTTACGTTATCGATTAGCGATGAGTGATAACCTGACCGAAAGCGAACAGCAGGCGTTACAT
CATGATATCGGTCAATTGGAAGCACTTATTGATGAACTACTGACCTATGCACGTTTGGATCGCCCACAAGTTTCCCTCAA
TCTGGAACCTATCGACCTACCGGCCTGGCTAACAACCAAAATCATGGATATCCGCTCGATTCATCAAGAGCGTGAGATCG
AACTTGATATTCCCCACCGTGGTGATTTTGGTGCCGTTGACCTGCGTCTGATGGAGCGAGTGCTGGATAACCTGGTCAAT
AATGCCCTACGCTATTCACATCAACGGCTGCGCATAGGGTTATGGTTCGATGGCGATAACGCCTGCCTACAGGTTGAAGA
TGACGGCCCCGGTATCCCGCCAGAAGAACGGACACGAATTTTTGAACCCTTTGTTCGTTTAGACCCCAGCCGTGATCGCG
CCACTGGTGGCTGTGGATTGGGGCTGGCCATTGTCCATTCGATTGCACTGGCCTATCAAGGCAGTATCTCTGTCAATACC
AGTCCATTAGGTGGGGCCAGTTTCCGCTTTAGTTGGCCGATAAAAACACATTTGCCTCTATCCGCTGATCAGAATTAA

Upstream 100 bases:

>100_bases
CCCATTCCGGATCAAAACGGTGCGTAACAAAGGTTATTTGTTTGCGCCCAATGCCTGGGAGTCCAAGCAGTAATGAGAAA
GCTATTTATTCAGTTTTTTT

Downstream 100 bases:

>100_bases
TGCGCGAACAGAACTAATTCTTCGCGAACTTACTATTTCGTGCACTTACTATTTCGTGCACTTGCTATTTCGCGAACCAC
TATGGAGTGATTTATGACAA

Product: sensor protein RstB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MLLFVCFMVMAMLVGLVYKVTAERAGRQSLDDLMKSSLSLMRSELREIPLKDWNKTIATLDLNLSFQLHIEPLDKRDLGA
QLNKRLRAGEIIALDDEYTFLQRIPRSHYVLAVGPVPYLFYLHQMRLLDLALLILIGLSLALPVFLWMRPHWKDLLKLEN
AAQRLGSGHLDERTHFDPTSSLSRLGVAFNQMADNINTLIVSKKLLIDGIAHELRTPLVRLRYRLAMSDNLTESEQQALH
HDIGQLEALIDELLTYARLDRPQVSLNLEPIDLPAWLTTKIMDIRSIHQEREIELDIPHRGDFGAVDLRLMERVLDNLVN
NALRYSHQRLRIGLWFDGDNACLQVEDDGPGIPPEERTRIFEPFVRLDPSRDRATGGCGLGLAIVHSIALAYQGSISVNT
SPLGGASFRFSWPIKTHLPLSADQN

Sequences:

>Translated_425_residues
MLLFVCFMVMAMLVGLVYKVTAERAGRQSLDDLMKSSLSLMRSELREIPLKDWNKTIATLDLNLSFQLHIEPLDKRDLGA
QLNKRLRAGEIIALDDEYTFLQRIPRSHYVLAVGPVPYLFYLHQMRLLDLALLILIGLSLALPVFLWMRPHWKDLLKLEN
AAQRLGSGHLDERTHFDPTSSLSRLGVAFNQMADNINTLIVSKKLLIDGIAHELRTPLVRLRYRLAMSDNLTESEQQALH
HDIGQLEALIDELLTYARLDRPQVSLNLEPIDLPAWLTTKIMDIRSIHQEREIELDIPHRGDFGAVDLRLMERVLDNLVN
NALRYSHQRLRIGLWFDGDNACLQVEDDGPGIPPEERTRIFEPFVRLDPSRDRATGGCGLGLAIVHSIALAYQGSISVNT
SPLGGASFRFSWPIKTHLPLSADQN
>Mature_425_residues
MLLFVCFMVMAMLVGLVYKVTAERAGRQSLDDLMKSSLSLMRSELREIPLKDWNKTIATLDLNLSFQLHIEPLDKRDLGA
QLNKRLRAGEIIALDDEYTFLQRIPRSHYVLAVGPVPYLFYLHQMRLLDLALLILIGLSLALPVFLWMRPHWKDLLKLEN
AAQRLGSGHLDERTHFDPTSSLSRLGVAFNQMADNINTLIVSKKLLIDGIAHELRTPLVRLRYRLAMSDNLTESEQQALH
HDIGQLEALIDELLTYARLDRPQVSLNLEPIDLPAWLTTKIMDIRSIHQEREIELDIPHRGDFGAVDLRLMERVLDNLVN
NALRYSHQRLRIGLWFDGDNACLQVEDDGPGIPPEERTRIFEPFVRLDPSRDRATGGCGLGLAIVHSIALAYQGSISVNT
SPLGGASFRFSWPIKTHLPLSADQN

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=419, Percent_Identity=69.2124105011933, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI1790346, Length=260, Percent_Identity=33.8461538461538, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1789808, Length=274, Percent_Identity=32.8467153284672, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788393, Length=273, Percent_Identity=32.2344322344322, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1786783, Length=309, Percent_Identity=29.126213592233, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786912, Length=229, Percent_Identity=31.8777292576419, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786600, Length=223, Percent_Identity=28.2511210762332, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789403, Length=449, Percent_Identity=26.5033407572383, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI145693157, Length=253, Percent_Identity=25.6916996047431, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI87081816, Length=252, Percent_Identity=27.3809523809524, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI48994928, Length=237, Percent_Identity=26.1603375527426, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082128, Length=292, Percent_Identity=26.7123287671233, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788279, Length=284, Percent_Identity=27.4647887323944, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790551, Length=216, Percent_Identity=30.0925925925926, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789149, Length=224, Percent_Identity=27.6785714285714, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1788549, Length=220, Percent_Identity=26.3636363636364, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788713, Length=230, Percent_Identity=23.9130434782609, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1790861, Length=214, Percent_Identity=29.4392523364486, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1787374, Length=327, Percent_Identity=25.6880733944954, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48251; Mature: 48251

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLFVCFMVMAMLVGLVYKVTAERAGRQSLDDLMKSSLSLMRSELREIPLKDWNKTIATL
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
DLNLSFQLHIEPLDKRDLGAQLNKRLRAGEIIALDDEYTFLQRIPRSHYVLAVGPVPYLF
EEEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHCCCCCEEEEECCHHHHH
YLHQMRLLDLALLILIGLSLALPVFLWMRPHWKDLLKLENAAQRLGSGHLDERTHFDPTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
SLSRLGVAFNQMADNINTLIVSKKLLIDGIAHELRTPLVRLRYRLAMSDNLTESEQQALH
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
HDIGQLEALIDELLTYARLDRPQVSLNLEPIDLPAWLTTKIMDIRSIHQEREIELDIPHR
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCC
GDFGAVDLRLMERVLDNLVNNALRYSHQRLRIGLWFDGDNACLQVEDDGPGIPPEERTRI
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEECCCCCCCCHHHHHH
FEPFVRLDPSRDRATGGCGLGLAIVHSIALAYQGSISVNTSPLGGASFRFSWPIKTHLPL
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEECCCEECCCC
SADQN
CCCCC
>Mature Secondary Structure
MLLFVCFMVMAMLVGLVYKVTAERAGRQSLDDLMKSSLSLMRSELREIPLKDWNKTIATL
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
DLNLSFQLHIEPLDKRDLGAQLNKRLRAGEIIALDDEYTFLQRIPRSHYVLAVGPVPYLF
EEEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHCCCCCEEEEECCHHHHH
YLHQMRLLDLALLILIGLSLALPVFLWMRPHWKDLLKLENAAQRLGSGHLDERTHFDPTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
SLSRLGVAFNQMADNINTLIVSKKLLIDGIAHELRTPLVRLRYRLAMSDNLTESEQQALH
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
HDIGQLEALIDELLTYARLDRPQVSLNLEPIDLPAWLTTKIMDIRSIHQEREIELDIPHR
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCC
GDFGAVDLRLMERVLDNLVNNALRYSHQRLRIGLWFDGDNACLQVEDDGPGIPPEERTRI
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEECCCCCCCCHHHHHH
FEPFVRLDPSRDRATGGCGLGLAIVHSIALAYQGSISVNTSPLGGASFRFSWPIKTHLPL
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEECCCEECCCC
SADQN
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]