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The map label for this gene is topA [H]

Identifier: 218929315

GI number: 218929315

Start: 2491024

End: 2493639

Strand: Direct

Name: topA [H]

Synonym: YPO2218

Alternate gene names: 218929315

Gene position: 2491024-2493639 (Clockwise)

Preceding gene: 218929314

Following gene: 218929316

Centisome position: 53.53

GC content: 48.32

Gene sequence:

>2616_bases
ATGGGTAAAGCTCTCGTAATAGTTGAGTCCCCGGCAAAAGCCAAAACTATTAATAAATACTTAGGGAATGATTACGTGGT
TAAGTCCAGCGTCGGTCACATTCGTGATTTGCCGACCAGTGGCTCAGCCAGTAAAAAGAGCGCTAACTCAGCTGAAGAAA
AAGCCAAAAAAGACGATAAACCCAAAAAGAAAGTGAAGAAAGACGAAAAGGTTGCCTTAGTCAACCGTATGGGGGTCGAT
CCTTATCATGGCTGGAAAGCGCAGTATGAAATTTTGTCTGGCAAAGAGAAAGTGGTTGCCGAGCTGAAAGCATTAGCAGA
AAACGCGGATCATATCTATCTCGCAACCGACCTTGATCGCGAAGGTGAGGCTATTGCCTGGCATTTGCGGGAAGTGATCG
GTGGGGACGATAAACGTTTTAGCCGCGTTGTCTTTAACGAAATCACCAAGAATGCGATTCAGCAGGCGTTTAATCAGCCT
GGTGAACTGAACATCAGCCGTGTAAATGCACAACAAGCCCGGCGTTTTATGGATCGGGTTGTTGGCTATATGGTCTCTCC
ACTGTTGTGGAAGAAAATTGCCCGTGGCCTGTCAGCTGGCCGTGTACAGTCAGTTGCTGTGCGCCTTGTGGTTGAACGTG
AAAAAGAGATTAAAGCATTCGTTCCTGAAGAATATTGGGAGCTACATGCAGATTTAGTGGCTAAAGGTGACATTCCGATT
CAAATGGAAGTCACTCATGCCCATGGCAAGCCATTTAAACCGGTAAATCGTGAGCAAACACATGCAGCGCTGACGTTACT
GGAAAATGCCCGTTATAAGGTGTTGGATCGTGAAGATAAACCGACCAGCAGTAAGCCCAGCGCACCCTTCATTACCTCCA
CCTTACAGCAGGCGGCAAGTACCCGTCTGAGTTTTGGTGTGAAAAAAACCATGATGATGGCACAGCGCCTGTATGAAGCG
GGTCATATCACCTACATGCGTACCGACTCGACTAATTTGAGTCAGGATGCATTGACGATGGTACGTGGTTATATCGGCGA
TAATTTTGGTGATAAGTATCTGCCTGCAACGCCTAACCAATACAGCAGCAAAGAGAATTCGCAGGAAGCGCATGAGGCCA
TCCGCCCTTCAGATGTCAATGTGTTAGCTGAGCAGTTGAAAGATATGGAAGCGGATGCCCAGAGGTTGTACCAATTGATT
TGGCGTCAGTTTGTGGCGTGCCAGATGCCTCCGGCCAAGTATGACTCGACCACACTGACAGTGCAGGCGGGGGATTTCCA
ACTGCGCGCCAAAGGCCGCACATTGCGCTTTGATGGTTGGACCAAGGTTATGCCTGCGCTGCGTAAAGGTGATGAAGATC
GCACCTTGCCGGTGATTGAAGTGGGCAGTGATTTAGACTTACAAAAACTGATTCCGAGCCAGCATTTTACCAAGCCACCG
GCTCGTTATAGTGAAGCATCATTAGTGAAAGAGCTGGAAAAACGGGGTATTGGCCGTCCATCGACTTATGCCTCTATTAT
TTCGACCATTCAAGATCGTGGCTATGTGCGGGTAGAAAACCGTCGCTTCTACGCAGAGAAAATGGGTGAAATTGTGACAG
ACCGCTTGGAAGAAAACTTCCGCGAGCTGATGAATTACGATTTCACGGCACGAATGGAAAGCGGTTTGGATCAAGTCGCC
AATGATCAGGCCGAATGGAAAGCGGTGCTGGATGGCTTCTTTGCTGAATTCAGCGAGCAGCTTGAAAAGGCAGAAAAAGA
TCCTGAAGAAGGAGGAATGCGCCCGAATCAGATGGTGATGACCAGCATTGATTGCCCAACCTGTGGCCGTCAGATGGGGA
TCCGTACTGCGAGTACTGGGGTGTTCCTTGGCTGTTCCGGTTATGCATTGCCGCCTAAAGAGCGCTGTAAAACCACCATT
AACTTGGTGCCGGACGCTGAGATTTTGAATATTCTGGAGGGTGATGATGCGGAGACTAACGCATTACGTGCCCGCCGCCG
TTGCCAGAAATGTGGTACTGCGATGGATAGCTACCTTATCGATAATCAACGCAAGTTACATGTTTGCGGGAATAACCCAG
CGTGTGATGGCTATGAAATCGAAGCAGGTGAGTTCCGGATAAAAGGCTATGAAGGCCCAATTGTTGAGTGCGAGAAATGT
GGTTCTGAAATGCACCTGAAAATGGGGCGCTTCGGTAAGTACATGGGCTGCACCAATGACGAGTGTAAGAATACCCGCAA
GATTCTGCGTAGCGGTGAAGTTGCACCACCGAAAGAAGATCCGGTACCATTGTTAGAGTTGCCTTGCGAGAAATCAGATG
CTTATTTCGTCCTACGAGATGGCGCAGCTGGCGTGTTCTTGGCAGCCAATACATTCCCTAAATCGCGTGAAACCCGTGCA
CCGCTGGTAGAAGAATTGGTTCGCTTTAAAGACCGTTTGCCTGAGAAGTTGCGTTATCTGGCTGATGCCCCTGTGGTTGA
TAAAGAGGGTAATAAAACCCTGGTCCGTTTCAGCCGTAAAACGAAGCAACAGTATGTTTCATCAGAGAAAGATGGCAAAG
CAACAGGCTGGTCTGCTTTCTATATTGATGGTAAATGGGTTGAGGCAAAGAAATAA

Upstream 100 bases:

>100_bases
TCCTAAAAAAGTACCGCAATATAAAAAAGAGATTCAGAATGCTGCGGCACAGCAAGATAAAAACCTTCTTTTAGAAGAAA
TAATTTAGGTAAAGGTAATT

Downstream 100 bases:

>100_bases
ATTCAACCTAAAGCGAATACTTAGGCTCGTTGTAGTCTATTGCTTCTGACCTCGGTAAAGCCAGCCTTCGCGGCTGGTTT
TTTATTGGGCTAATTTATCC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 871; Mature: 870

Protein sequence:

>871_residues
MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSANSAEEKAKKDDKPKKKVKKDEKVALVNRMGVD
PYHGWKAQYEILSGKEKVVAELKALAENADHIYLATDLDREGEAIAWHLREVIGGDDKRFSRVVFNEITKNAIQQAFNQP
GELNISRVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREKEIKAFVPEEYWELHADLVAKGDIPI
QMEVTHAHGKPFKPVNREQTHAALTLLENARYKVLDREDKPTSSKPSAPFITSTLQQAASTRLSFGVKKTMMMAQRLYEA
GHITYMRTDSTNLSQDALTMVRGYIGDNFGDKYLPATPNQYSSKENSQEAHEAIRPSDVNVLAEQLKDMEADAQRLYQLI
WRQFVACQMPPAKYDSTTLTVQAGDFQLRAKGRTLRFDGWTKVMPALRKGDEDRTLPVIEVGSDLDLQKLIPSQHFTKPP
ARYSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTARMESGLDQVA
NDQAEWKAVLDGFFAEFSEQLEKAEKDPEEGGMRPNQMVMTSIDCPTCGRQMGIRTASTGVFLGCSGYALPPKERCKTTI
NLVPDAEILNILEGDDAETNALRARRRCQKCGTAMDSYLIDNQRKLHVCGNNPACDGYEIEAGEFRIKGYEGPIVECEKC
GSEMHLKMGRFGKYMGCTNDECKNTRKILRSGEVAPPKEDPVPLLELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRA
PLVEELVRFKDRLPEKLRYLADAPVVDKEGNKTLVRFSRKTKQQYVSSEKDGKATGWSAFYIDGKWVEAKK

Sequences:

>Translated_871_residues
MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSANSAEEKAKKDDKPKKKVKKDEKVALVNRMGVD
PYHGWKAQYEILSGKEKVVAELKALAENADHIYLATDLDREGEAIAWHLREVIGGDDKRFSRVVFNEITKNAIQQAFNQP
GELNISRVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREKEIKAFVPEEYWELHADLVAKGDIPI
QMEVTHAHGKPFKPVNREQTHAALTLLENARYKVLDREDKPTSSKPSAPFITSTLQQAASTRLSFGVKKTMMMAQRLYEA
GHITYMRTDSTNLSQDALTMVRGYIGDNFGDKYLPATPNQYSSKENSQEAHEAIRPSDVNVLAEQLKDMEADAQRLYQLI
WRQFVACQMPPAKYDSTTLTVQAGDFQLRAKGRTLRFDGWTKVMPALRKGDEDRTLPVIEVGSDLDLQKLIPSQHFTKPP
ARYSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTARMESGLDQVA
NDQAEWKAVLDGFFAEFSEQLEKAEKDPEEGGMRPNQMVMTSIDCPTCGRQMGIRTASTGVFLGCSGYALPPKERCKTTI
NLVPDAEILNILEGDDAETNALRARRRCQKCGTAMDSYLIDNQRKLHVCGNNPACDGYEIEAGEFRIKGYEGPIVECEKC
GSEMHLKMGRFGKYMGCTNDECKNTRKILRSGEVAPPKEDPVPLLELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRA
PLVEELVRFKDRLPEKLRYLADAPVVDKEGNKTLVRFSRKTKQQYVSSEKDGKATGWSAFYIDGKWVEAKK
>Mature_870_residues
GKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSANSAEEKAKKDDKPKKKVKKDEKVALVNRMGVDP
YHGWKAQYEILSGKEKVVAELKALAENADHIYLATDLDREGEAIAWHLREVIGGDDKRFSRVVFNEITKNAIQQAFNQPG
ELNISRVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREKEIKAFVPEEYWELHADLVAKGDIPIQ
MEVTHAHGKPFKPVNREQTHAALTLLENARYKVLDREDKPTSSKPSAPFITSTLQQAASTRLSFGVKKTMMMAQRLYEAG
HITYMRTDSTNLSQDALTMVRGYIGDNFGDKYLPATPNQYSSKENSQEAHEAIRPSDVNVLAEQLKDMEADAQRLYQLIW
RQFVACQMPPAKYDSTTLTVQAGDFQLRAKGRTLRFDGWTKVMPALRKGDEDRTLPVIEVGSDLDLQKLIPSQHFTKPPA
RYSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTARMESGLDQVAN
DQAEWKAVLDGFFAEFSEQLEKAEKDPEEGGMRPNQMVMTSIDCPTCGRQMGIRTASTGVFLGCSGYALPPKERCKTTIN
LVPDAEILNILEGDDAETNALRARRRCQKCGTAMDSYLIDNQRKLHVCGNNPACDGYEIEAGEFRIKGYEGPIVECEKCG
SEMHLKMGRFGKYMGCTNDECKNTRKILRSGEVAPPKEDPVPLLELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAP
LVEELVRFKDRLPEKLRYLADAPVVDKEGNKTLVRFSRKTKQQYVSSEKDGKATGWSAFYIDGKWVEAKK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=580, Percent_Identity=26.2068965517241, Blast_Score=140, Evalue=7e-33,
Organism=Homo sapiens, GI4507635, Length=502, Percent_Identity=25.8964143426295, Blast_Score=135, Evalue=2e-31,
Organism=Escherichia coli, GI1787529, Length=871, Percent_Identity=85.1894374282434, Blast_Score=1564, Evalue=0.0,
Organism=Escherichia coli, GI1788061, Length=549, Percent_Identity=23.4972677595628, Blast_Score=106, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI32563869, Length=589, Percent_Identity=25.4668930390492, Blast_Score=137, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=492, Percent_Identity=25.2032520325203, Blast_Score=122, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6323263, Length=486, Percent_Identity=25.9259259259259, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24585251, Length=641, Percent_Identity=24.9609984399376, Blast_Score=132, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24640096, Length=495, Percent_Identity=26.8686868686869, Blast_Score=121, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR013263
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 97977; Mature: 97846

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSANSAEEKAKKDDK
CCCEEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCCCCCCCHHHHHHHCCCC
PKKKVKKDEKVALVNRMGVDPYHGWKAQYEILSGKEKVVAELKALAENADHIYLATDLDR
HHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCC
EGEAIAWHLREVIGGDDKRFSRVVFNEITKNAIQQAFNQPGELNISRVNAQQARRFMDRV
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHH
VGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREKEIKAFVPEEYWELHADLVAKGDIPI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCE
QMEVTHAHGKPFKPVNREQTHAALTLLENARYKVLDREDKPTSSKPSAPFITSTLQQAAS
EEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHH
TRLSFGVKKTMMMAQRLYEAGHITYMRTDSTNLSQDALTMVRGYIGDNFGDKYLPATPNQ
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
YSSKENSQEAHEAIRPSDVNVLAEQLKDMEADAQRLYQLIWRQFVACQMPPAKYDSTTLT
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
VQAGDFQLRAKGRTLRFDGWTKVMPALRKGDEDRTLPVIEVGSDLDLQKLIPSQHFTKPP
EEECCEEEEECCCEEEECCHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHCCCCCCCCCC
ARYSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENF
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHH
RELMNYDFTARMESGLDQVANDQAEWKAVLDGFFAEFSEQLEKAEKDPEEGGMRPNQMVM
HHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEE
TSIDCPTCGRQMGIRTASTGVFLGCSGYALPPKERCKTTINLVPDAEILNILEGDDAETN
EECCCCCCCHHHCCEEECCCEEEECCCCCCCCHHHHCCEEECCCCHHHHHHHCCCCCHHH
ALRARRRCQKCGTAMDSYLIDNQRKLHVCGNNPACDGYEIEAGEFRIKGYEGPIVECEKC
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECCCCEEEECCCCCCHHHHHC
GSEMHLKMGRFGKYMGCTNDECKNTRKILRSGEVAPPKEDPVPLLELPCEKSDAYFVLRD
CCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCEEEEEC
GAAGVFLAANTFPKSRETRAPLVEELVRFKDRLPEKLRYLADAPVVDKEGNKTLVRFSRK
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCHHHHHHHH
TKQQYVSSEKDGKATGWSAFYIDGKWVEAKK
HHHHHHCCCCCCCCCCEEEEEECCEEECCCC
>Mature Secondary Structure 
GKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSANSAEEKAKKDDK
CCEEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCCCCCCCHHHHHHHCCCC
PKKKVKKDEKVALVNRMGVDPYHGWKAQYEILSGKEKVVAELKALAENADHIYLATDLDR
HHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCC
EGEAIAWHLREVIGGDDKRFSRVVFNEITKNAIQQAFNQPGELNISRVNAQQARRFMDRV
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHH
VGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREKEIKAFVPEEYWELHADLVAKGDIPI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCE
QMEVTHAHGKPFKPVNREQTHAALTLLENARYKVLDREDKPTSSKPSAPFITSTLQQAAS
EEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHH
TRLSFGVKKTMMMAQRLYEAGHITYMRTDSTNLSQDALTMVRGYIGDNFGDKYLPATPNQ
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
YSSKENSQEAHEAIRPSDVNVLAEQLKDMEADAQRLYQLIWRQFVACQMPPAKYDSTTLT
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
VQAGDFQLRAKGRTLRFDGWTKVMPALRKGDEDRTLPVIEVGSDLDLQKLIPSQHFTKPP
EEECCEEEEECCCEEEECCHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHCCCCCCCCCC
ARYSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENF
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHH
RELMNYDFTARMESGLDQVANDQAEWKAVLDGFFAEFSEQLEKAEKDPEEGGMRPNQMVM
HHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEE
TSIDCPTCGRQMGIRTASTGVFLGCSGYALPPKERCKTTINLVPDAEILNILEGDDAETN
EECCCCCCCHHHCCEEECCCEEEECCCCCCCCHHHHCCEEECCCCHHHHHHHCCCCCHHH
ALRARRRCQKCGTAMDSYLIDNQRKLHVCGNNPACDGYEIEAGEFRIKGYEGPIVECEKC
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECCCCEEEECCCCCCHHHHHC
GSEMHLKMGRFGKYMGCTNDECKNTRKILRSGEVAPPKEDPVPLLELPCEKSDAYFVLRD
CCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCEEEEEC
GAAGVFLAANTFPKSRETRAPLVEELVRFKDRLPEKLRYLADAPVVDKEGNKTLVRFSRK
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCHHHHHHHH
TKQQYVSSEKDGKATGWSAFYIDGKWVEAKK
HHHHHHCCCCCCCCCCEEEEEECCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3029379; 9097039; 9278503; 3032952; 8114910; 7779808 [H]