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The map label for this gene is hns [H]

Identifier: 218929274

GI number: 218929274

Start: 2448157

End: 2448555

Strand: Reverse

Name: hns [H]

Synonym: YPO2175

Alternate gene names: 218929274

Gene position: 2448555-2448157 (Counterclockwise)

Preceding gene: 218929276

Following gene: 218929271

Centisome position: 52.61

GC content: 43.36

Gene sequence:

>399_bases
ATGAGCGAAGCGTTAAAAATTCTTAACAACATCCGTACTCTACGTGCTCAAGCGCGCGAATGCACTCTTGAAACACTAGA
AGAAATGCTTGAGAAGCTTGAAGTGGTCGTTAATGAACGCCGCGAAGAAGAATCTCAATCACAAGCAGAAATTGAAGAGC
GTGCACGTAAATTGCAACAATACCGTGAAATGCTTATTGCTGACGGTATTGATCCAAATGAACTGCTACAAGCTTCAGCT
GCTGCTAAAGCTGCTGGTAAAGCAAAACGTCCTGCTAAATATCAGTATAAAGATGAGAATGGCGAACTGAAAACTTGGAC
AGGCCAAGGCCGTACTCCTGCAGTGATTAAGAAGGCCATTGAAGAGCAAGGCAAATCACTGGATGACTTCCTGTTGTAA

Upstream 100 bases:

>100_bases
AATTCTAGTATGCTAATCAAATTGGCTATTGCAGAAATTAAAATAGCACTCTATTATTATCCAGACGCCCCCCCACCAAT
TATAATTTGAGACTAGGACA

Downstream 100 bases:

>100_bases
TAGTTTTATTTCTTTAGCTATTACTATCGAAACACCCTCCTATTAATAGGAGGGTGTTTTTTTGTCTTTCATTTAACTGA
CCGATGTCATCACCGTTGAA

Product: global DNA-binding transcriptional dual regulator H-NS

Products: NA

Alternate protein names: Histone-like protein HLP-II [H]

Number of amino acids: Translated: 132; Mature: 131

Protein sequence:

>132_residues
MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEESQSQAEIEERARKLQQYREMLIADGIDPNELLQASA
AAKAAGKAKRPAKYQYKDENGELKTWTGQGRTPAVIKKAIEEQGKSLDDFLL

Sequences:

>Translated_132_residues
MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEESQSQAEIEERARKLQQYREMLIADGIDPNELLQASA
AAKAAGKAKRPAKYQYKDENGELKTWTGQGRTPAVIKKAIEEQGKSLDDFLL
>Mature_131_residues
SEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEESQSQAEIEERARKLQQYREMLIADGIDPNELLQASAA
AKAAGKAKRPAKYQYKDENGELKTWTGQGRTPAVIKKAIEEQGKSLDDFLL

Specific function: H-NS binds tightly to ds-DNA, increases its thermal stability and inhibits transcription. It also binds to ss-DNA and RNA but with a much lower affinity. H-NS has possible histone-like function. May be a global transcriptional regulator through its abilit

COG id: COG2916

COG function: function code R; DNA-binding protein H-NS

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the histone-like protein H-NS family [H]

Homologues:

Organism=Escherichia coli, GI1787489, Length=135, Percent_Identity=84.4444444444444, Blast_Score=201, Evalue=2e-53,
Organism=Escherichia coli, GI1789023, Length=135, Percent_Identity=60.7407407407407, Blast_Score=134, Evalue=3e-33,

Paralogues:

None

Copy number: 820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1780 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 1020 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 4632 Molecules/Cell In: Gr

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001801 [H]

Pfam domain/function: PF00816 Histone_HNS [H]

EC number: NA

Molecular weight: Translated: 15018; Mature: 14887

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEESQSQAEIEERARKLQQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YREMLIADGIDPNELLQASAAAKAAGKAKRPAKYQYKDENGELKTWTGQGRTPAVIKKAI
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHH
EEQGKSLDDFLL
HHHCCCHHHHCC
>Mature Secondary Structure 
SEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEESQSQAEIEERARKLQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YREMLIADGIDPNELLQASAAAKAAGKAKRPAKYQYKDENGELKTWTGQGRTPAVIKKAI
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHH
EEQGKSLDDFLL
HHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2494066 [H]