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The map label for this gene is mdtL [H]

Identifier: 218929249

GI number: 218929249

Start: 2416636

End: 2417838

Strand: Reverse

Name: mdtL [H]

Synonym: YPO2148

Alternate gene names: 218929249

Gene position: 2417838-2416636 (Counterclockwise)

Preceding gene: 218929250

Following gene: 218929247

Centisome position: 51.95

GC content: 50.04

Gene sequence:

>1203_bases
ATGCAAAAATTCCTTTTCCTGCTATTAAGCTTGATTTTACTTGGCCCTTTGGGGATCGATCTCTATCTACCGACCATTCC
AGCCATTGCAAAAGGGCTGAATAGTAGTGAGTCTCTGATTCAATCAACTATTGCGCTGTTTATTCTCGTGCTGGGTATCG
GGCAACTGATTGCTGGGCCATTGGTTGATAAATATGGCCGTAAGCCAATCGCTATTATCGGTATTGTTCTTTACATGCTG
GGTGCCGCGATGGCCGCTCTGGCCGTGGATCCAGTCATGTTCGTCAGCTCCCGCTTGTTACAAGGTGTCTCGGTATGCTG
CACAGCCGTGGTGGCCTTCAGTGGCGTCCGTGACCGCCTTAGCGGCAATGAAGCCGCACGTGCTTTTGGCTTCCTTAATG
GCACGTTGAATATTGTCCCCGCATTGGCCCCGCTATTAGGTGGTTTACTGGCCGAAGCCTTTGGCTGGCGGGCACCGTTC
TGGTTCCTGGCATTATACGGATTACTGGTTTTAGTCTTAATCATTATGTTCTTACCCGAAACCCGCCCGGCGAATACTCA
ACCAATAAATGGCCTGCCAATAAAAAACTACCTGCGTATTTTGCGTGATAGCCGCTTTTTGGTATTTGCTCTGGTCAATG
CAGGAGCCATGGGCATGGCATTAACCTATGTATCATTGGCACCGAATGTATTGATGGTTGATGCAGGCCTCAGCCCGTTA
CAATTTTCGCTGGCATTTGGGGCTAATGGCTTCTGGATTATGGCAGTCAGTTTTTTTGCTAACCGGATTATTCAAAAAGT
GGGCCGCCCTACCTGCCTGATGATCGGTAGTTTATTGATGGCCTTAGGCTGTTTTGGCTTGCTATTTGGTCTGACACAAC
TGTCCGTTGATGTGCAGCATCACTGGCTGGTTTATATGTTACCGGTTGCCAGCGCCTGTGCCGGTTTAGCCTTTATGATG
GGGCCTGCCACCAGTTATGCTCTAGAACCCTATGCGAATGAAGCGGGTGTCGCCTCAGCGTTAGTGGGGTTTGTTCAAAT
GGCTGGGGGCGCGGCATTAGGCCTGTTGGCGATGGCACTGCCGATTGAACCTAAGTTGTCACTGGCTATCGTGATGCTGG
CTGTTTACTGGCGTGGCAAGCGCGCCGAGCCAGTAAACATGTCCGCGGCAAGTTGGAAGAAATACACTAACAACGGCAAA
TAA

Upstream 100 bases:

>100_bases
GACAGTGTCACAAAATCACGCTTAGCACTTATGGGTTTCCTATTTACGGGTAAAGCCCTTATTCTTGTCGCCTTTGTAAT
TTGGATGCGATTTCTTTTTC

Downstream 100 bases:

>100_bases
TCGCGTTACACGATATTAACGGATGGGGTGAATATCGTGTAACGGGGCAATATTCAGTGGCTGAAAACCTGCGCGGTAAC
GCGCTACCGATAAATCATCA

Product: putative multidrug resistance protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MQKFLFLLLSLILLGPLGIDLYLPTIPAIAKGLNSSESLIQSTIALFILVLGIGQLIAGPLVDKYGRKPIAIIGIVLYML
GAAMAALAVDPVMFVSSRLLQGVSVCCTAVVAFSGVRDRLSGNEAARAFGFLNGTLNIVPALAPLLGGLLAEAFGWRAPF
WFLALYGLLVLVLIIMFLPETRPANTQPINGLPIKNYLRILRDSRFLVFALVNAGAMGMALTYVSLAPNVLMVDAGLSPL
QFSLAFGANGFWIMAVSFFANRIIQKVGRPTCLMIGSLLMALGCFGLLFGLTQLSVDVQHHWLVYMLPVASACAGLAFMM
GPATSYALEPYANEAGVASALVGFVQMAGGAALGLLAMALPIEPKLSLAIVMLAVYWRGKRAEPVNMSAASWKKYTNNGK

Sequences:

>Translated_400_residues
MQKFLFLLLSLILLGPLGIDLYLPTIPAIAKGLNSSESLIQSTIALFILVLGIGQLIAGPLVDKYGRKPIAIIGIVLYML
GAAMAALAVDPVMFVSSRLLQGVSVCCTAVVAFSGVRDRLSGNEAARAFGFLNGTLNIVPALAPLLGGLLAEAFGWRAPF
WFLALYGLLVLVLIIMFLPETRPANTQPINGLPIKNYLRILRDSRFLVFALVNAGAMGMALTYVSLAPNVLMVDAGLSPL
QFSLAFGANGFWIMAVSFFANRIIQKVGRPTCLMIGSLLMALGCFGLLFGLTQLSVDVQHHWLVYMLPVASACAGLAFMM
GPATSYALEPYANEAGVASALVGFVQMAGGAALGLLAMALPIEPKLSLAIVMLAVYWRGKRAEPVNMSAASWKKYTNNGK
>Mature_400_residues
MQKFLFLLLSLILLGPLGIDLYLPTIPAIAKGLNSSESLIQSTIALFILVLGIGQLIAGPLVDKYGRKPIAIIGIVLYML
GAAMAALAVDPVMFVSSRLLQGVSVCCTAVVAFSGVRDRLSGNEAARAFGFLNGTLNIVPALAPLLGGLLAEAFGWRAPF
WFLALYGLLVLVLIIMFLPETRPANTQPINGLPIKNYLRILRDSRFLVFALVNAGAMGMALTYVSLAPNVLMVDAGLSPL
QFSLAFGANGFWIMAVSFFANRIIQKVGRPTCLMIGSLLMALGCFGLLFGLTQLSVDVQHHWLVYMLPVASACAGLAFMM
GPATSYALEPYANEAGVASALVGFVQMAGGAALGLLAMALPIEPKLSLAIVMLAVYWRGKRAEPVNMSAASWKKYTNNGK

Specific function: Confers resistance to chloramphenicol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtL (TC 2.A.1.2.22) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790146, Length=351, Percent_Identity=33.6182336182336, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1788509, Length=358, Percent_Identity=27.6536312849162, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87082312, Length=355, Percent_Identity=28.169014084507, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI48994889, Length=246, Percent_Identity=30.8943089430894, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790794, Length=252, Percent_Identity=27.3809523809524, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081738, Length=192, Percent_Identity=26.5625, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787065, Length=233, Percent_Identity=21.0300429184549, Blast_Score=62, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=166, Percent_Identity=26.5060240963855, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6322071, Length=164, Percent_Identity=30.4878048780488, Blast_Score=73, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6319517, Length=182, Percent_Identity=25.2747252747253, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42532; Mature: 42532

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKFLFLLLSLILLGPLGIDLYLPTIPAIAKGLNSSESLIQSTIALFILVLGIGQLIAGP
CHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVDKYGRKPIAIIGIVLYMLGAAMAALAVDPVMFVSSRLLQGVSVCCTAVVAFSGVRDRL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGNEAARAFGFLNGTLNIVPALAPLLGGLLAEAFGWRAPFWFLALYGLLVLVLIIMFLPE
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
TRPANTQPINGLPIKNYLRILRDSRFLVFALVNAGAMGMALTYVSLAPNVLMVDAGLSPL
CCCCCCCCCCCCCHHHHHHHHHCCCEEEEHHHCCCHHHHHHHHHHHCCCEEEEECCCCCC
QFSLAFGANGFWIMAVSFFANRIIQKVGRPTCLMIGSLLMALGCFGLLFGLTQLSVDVQH
EEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HWLVYMLPVASACAGLAFMMGPATSYALEPYANEAGVASALVGFVQMAGGAALGLLAMAL
HHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHC
PIEPKLSLAIVMLAVYWRGKRAEPVNMSAASWKKYTNNGK
CCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MQKFLFLLLSLILLGPLGIDLYLPTIPAIAKGLNSSESLIQSTIALFILVLGIGQLIAGP
CHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVDKYGRKPIAIIGIVLYMLGAAMAALAVDPVMFVSSRLLQGVSVCCTAVVAFSGVRDRL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGNEAARAFGFLNGTLNIVPALAPLLGGLLAEAFGWRAPFWFLALYGLLVLVLIIMFLPE
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
TRPANTQPINGLPIKNYLRILRDSRFLVFALVNAGAMGMALTYVSLAPNVLMVDAGLSPL
CCCCCCCCCCCCCHHHHHHHHHCCCEEEEHHHCCCHHHHHHHHHHHCCCEEEEECCCCCC
QFSLAFGANGFWIMAVSFFANRIIQKVGRPTCLMIGSLLMALGCFGLLFGLTQLSVDVQH
EEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HWLVYMLPVASACAGLAFMMGPATSYALEPYANEAGVASALVGFVQMAGGAALGLLAMAL
HHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHC
PIEPKLSLAIVMLAVYWRGKRAEPVNMSAASWKKYTNNGK
CCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA