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The map label for this gene is cusS [H]

Identifier: 218929110

GI number: 218929110

Start: 2272373

End: 2273764

Strand: Reverse

Name: cusS [H]

Synonym: YPO2000

Alternate gene names: 218929110

Gene position: 2273764-2272373 (Counterclockwise)

Preceding gene: 218929111

Following gene: 218929109

Centisome position: 48.86

GC content: 51.8

Gene sequence:

>1392_bases
GTGATCATGATTAAGCGTTCTATCTCTGGGCGGCTGGCGCTAATGTTTGCCTTGGCCTCCATACTGATAGTTTCGGCTCT
CGGTATTGTACTGCGAAGCTCCTTGCATAACTCATTGCAAAAGCAGATGCATAACGAATTGCAATTCACGGAGTCGCTGA
TGGCACCGTGGATCCTCTCCCGTACCACGCTGGACGGCTGGCAAACGTTGGCGACCAAATTCACCGATTTGGCCGCTGCT
GAGGGCGGCCGCGTACAATACTGGGTCCTCAGTGATGACCCCCAGCTTCAGGTGGGCGGCCCGGCACCCCGTGGTGTAAC
CTGGGCTTCGTTAAGCAAAGGATTCAGCAAAGTACCCGGTGCGACAGACGAGTCATGCTCCCTATTTCTACTGATGTCGG
AAATTCCTGCCCAAGGTGAGCGCCCCGCTCTGCGCTATGTGGTCGCTATCGATTCCACGCCGTATATGGGTACTCTGAAT
GAATTTACCCGCACGCTGATCGTCATTACTGCGATTGGCGTCTTATTCGTGGCACTGCTGGGGTTCGTGATTGCCAGAAT
AGGTATGCGCCCTGTTAATAAACTCAGCGAACAGGCCCAGCACCTTGCGCCAGGAAATCACGGGCAGCGTCTGGATACCT
TAACTCTGCCGGATGAACTGCAAAAACTGGCCGTCGCATTCAACGGCGTGCTCGAACGGCAGGAAATCGCCTGGCGGCAA
CTTGAAAGTTTTAATGCTGATGTCGCACATGAACTGAGAACCCCGTTGACCAATCTGATGGGGCAAACGCAATTAGGTCT
ATCTCGTAAACGTGAAACGCATGAGCTCGAAGATTTACTGGAGTCCAATCTGGAAGAACTCGAGAGAATGACCTCTATTG
TTAACGATATGCTGTTCCTCTCCCATGCTCAAGCTGGGGAGCACGCAACCCAGTTAACCGACGTGTCTCTTCGGGAAGAG
ACCTATAAGACCACGGAGTATGTAGAGCCTTCATTTGCCGAAAAAAATTTGTCTATTCATGTTGAGGGGGATATCACTGC
ACGCATTGATCGGCGGCTTTTCCACCGCTCACTGGCGAATCTGCTAGAAAACAGCGCCCGCCATGCTGTTACTGGTACTA
CCGTGAAAGTGACGATCTCCAGAGAAAATAATCTGGCATCCATCGCGGTAGCCAACATTGGAGCTCCCATTGCGGCGGAA
CATCTAAACCGTTTGTTTGAGCGTTTTTATCGTGTTGATTCTTCCCGTGCACGCAGTGACACACACCATGGACTCGGGCT
CTCCATCGTCAGGGCGGTGGCGATAATGCATCGTGGAGATGTGTTTGCCAGTAGCCAGAATGGGATTAATACCTTTGGTC
TGACCCTTGCAATAACTGACCCTTATATATAG

Upstream 100 bases:

>100_bases
CGAAAATAGATACGCCCTACCCGGTCAAACTGCTGCATACAGTGCGAGGCATGGGGTATGTCCTTGAAACTCGCCCCGAT
CGCCAGACTAAATAGAGAGA

Downstream 100 bases:

>100_bases
ACAGTGAGTCCGTTGAAAAAGAAATGAGTCGTTATGTGAAGTCTGGCCTGGCTGAGCGAGGGTAACGTCTATGACAGTGT
ATTTTTGCATACAGGTTTAA

Product: putative two-component system sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MIMIKRSISGRLALMFALASILIVSALGIVLRSSLHNSLQKQMHNELQFTESLMAPWILSRTTLDGWQTLATKFTDLAAA
EGGRVQYWVLSDDPQLQVGGPAPRGVTWASLSKGFSKVPGATDESCSLFLLMSEIPAQGERPALRYVVAIDSTPYMGTLN
EFTRTLIVITAIGVLFVALLGFVIARIGMRPVNKLSEQAQHLAPGNHGQRLDTLTLPDELQKLAVAFNGVLERQEIAWRQ
LESFNADVAHELRTPLTNLMGQTQLGLSRKRETHELEDLLESNLEELERMTSIVNDMLFLSHAQAGEHATQLTDVSLREE
TYKTTEYVEPSFAEKNLSIHVEGDITARIDRRLFHRSLANLLENSARHAVTGTTVKVTISRENNLASIAVANIGAPIAAE
HLNRLFERFYRVDSSRARSDTHHGLGLSIVRAVAIMHRGDVFASSQNGINTFGLTLAITDPYI

Sequences:

>Translated_463_residues
MIMIKRSISGRLALMFALASILIVSALGIVLRSSLHNSLQKQMHNELQFTESLMAPWILSRTTLDGWQTLATKFTDLAAA
EGGRVQYWVLSDDPQLQVGGPAPRGVTWASLSKGFSKVPGATDESCSLFLLMSEIPAQGERPALRYVVAIDSTPYMGTLN
EFTRTLIVITAIGVLFVALLGFVIARIGMRPVNKLSEQAQHLAPGNHGQRLDTLTLPDELQKLAVAFNGVLERQEIAWRQ
LESFNADVAHELRTPLTNLMGQTQLGLSRKRETHELEDLLESNLEELERMTSIVNDMLFLSHAQAGEHATQLTDVSLREE
TYKTTEYVEPSFAEKNLSIHVEGDITARIDRRLFHRSLANLLENSARHAVTGTTVKVTISRENNLASIAVANIGAPIAAE
HLNRLFERFYRVDSSRARSDTHHGLGLSIVRAVAIMHRGDVFASSQNGINTFGLTLAITDPYI
>Mature_463_residues
MIMIKRSISGRLALMFALASILIVSALGIVLRSSLHNSLQKQMHNELQFTESLMAPWILSRTTLDGWQTLATKFTDLAAA
EGGRVQYWVLSDDPQLQVGGPAPRGVTWASLSKGFSKVPGATDESCSLFLLMSEIPAQGERPALRYVVAIDSTPYMGTLN
EFTRTLIVITAIGVLFVALLGFVIARIGMRPVNKLSEQAQHLAPGNHGQRLDTLTLPDELQKLAVAFNGVLERQEIAWRQ
LESFNADVAHELRTPLTNLMGQTQLGLSRKRETHELEDLLESNLEELERMTSIVNDMLFLSHAQAGEHATQLTDVSLREE
TYKTTEYVEPSFAEKNLSIHVEGDITARIDRRLFHRSLANLLENSARHAVTGTTVKVTISRENNLASIAVANIGAPIAAE
HLNRLFERFYRVDSSRARSDTHHGLGLSIVRAVAIMHRGDVFASSQNGINTFGLTLAITDPYI

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=322, Percent_Identity=35.0931677018634, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788279, Length=316, Percent_Identity=30.379746835443, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788393, Length=259, Percent_Identity=31.2741312741313, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786600, Length=250, Percent_Identity=28.8, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787894, Length=305, Percent_Identity=27.8688524590164, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790346, Length=265, Percent_Identity=29.0566037735849, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1789403, Length=289, Percent_Identity=26.643598615917, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI145693157, Length=212, Percent_Identity=27.3584905660377, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790551, Length=313, Percent_Identity=28.4345047923323, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786912, Length=254, Percent_Identity=26.7716535433071, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790861, Length=233, Percent_Identity=25.3218884120172, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51170; Mature: 51170

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMIKRSISGRLALMFALASILIVSALGIVLRSSLHNSLQKQMHNELQFTESLMAPWILS
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTTLDGWQTLATKFTDLAAAEGGRVQYWVLSDDPQLQVGGPAPRGVTWASLSKGFSKVPG
HCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCCHHHHHCCHHHCCC
ATDESCSLFLLMSEIPAQGERPALRYVVAIDSTPYMGTLNEFTRTLIVITAIGVLFVALL
CCCCCCEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GFVIARIGMRPVNKLSEQAQHLAPGNHGQRLDTLTLPDELQKLAVAFNGVLERQEIAWRQ
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCEECEECCHHHHHHHHHHHHHHHHHHHHHHHH
LESFNADVAHELRTPLTNLMGQTQLGLSRKRETHELEDLLESNLEELERMTSIVNDMLFL
HHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHAQAGEHATQLTDVSLREETYKTTEYVEPSFAEKNLSIHVEGDITARIDRRLFHRSLAN
HHHCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
LLENSARHAVTGTTVKVTISRENNLASIAVANIGAPIAAEHLNRLFERFYRVDSSRARSD
HHHCCCCCEEECCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHCCC
THHGLGLSIVRAVAIMHRGDVFASSQNGINTFGLTLAITDPYI
CCCCCHHHHHHHHHHHHCCCEEECCCCCCEEEEEEEEECCCCC
>Mature Secondary Structure
MIMIKRSISGRLALMFALASILIVSALGIVLRSSLHNSLQKQMHNELQFTESLMAPWILS
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTTLDGWQTLATKFTDLAAAEGGRVQYWVLSDDPQLQVGGPAPRGVTWASLSKGFSKVPG
HCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCCHHHHHCCHHHCCC
ATDESCSLFLLMSEIPAQGERPALRYVVAIDSTPYMGTLNEFTRTLIVITAIGVLFVALL
CCCCCCEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GFVIARIGMRPVNKLSEQAQHLAPGNHGQRLDTLTLPDELQKLAVAFNGVLERQEIAWRQ
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCEECEECCHHHHHHHHHHHHHHHHHHHHHHHH
LESFNADVAHELRTPLTNLMGQTQLGLSRKRETHELEDLLESNLEELERMTSIVNDMLFL
HHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHAQAGEHATQLTDVSLREETYKTTEYVEPSFAEKNLSIHVEGDITARIDRRLFHRSLAN
HHHCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
LLENSARHAVTGTTVKVTISRENNLASIAVANIGAPIAAEHLNRLFERFYRVDSSRARSD
HHHCCCCCEEECCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHCCC
THHGLGLSIVRAVAIMHRGDVFASSQNGINTFGLTLAITDPYI
CCCCCHHHHHHHHHHHHCCCEEECCCCCCEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]