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The map label for this gene is astE

Identifier: 218929080

GI number: 218929080

Start: 2233662

End: 2234654

Strand: Direct

Name: astE

Synonym: YPO1966

Alternate gene names: 218929080

Gene position: 2233662-2234654 (Clockwise)

Preceding gene: 218929079

Following gene: 218929081

Centisome position: 48.0

GC content: 48.04

Gene sequence:

>993_bases
ATGCTGGATTTTTTAGCGATAACCCTATCGGGTAAACCTCCACAAGTCATACAGGGTGAAACCGTTAATCTGAAATGGCA
ATGGCTCGGTGAGGGGATATTGACACTGGTTCCGCACCGCAGCTACACACAATCGGTGGTGATCTCTGCCGGTATCCACG
GCAATGAAACGGCACCCATTGAAATTTTGAATCAATTAGTCACCGATTTATTAGCGGGTCAACTGCCGCTGTCCGTCCGT
CTGTTAGTGTTATTGGGAAATCCACCGGCGATCCGCAAAGGGAAGCGGTACCTCAGCAATGACATTAACCGTATGTTTGG
TGGGCGTTATCAGCACTATACACCCAGTGACGAGACGCGAAGGGCGAGTACTCTGGAGCAACGGGTGATGGCATTTTTCC
AGGCAAGTCACACATCAGAGCGTTTGCATTATGATCTACACACCGCAATTCGGGGGTCCTATCATCCTCGCTTTGGTTTG
TTACCCTACCAGCAGACCCCTTATTCCGCTGCTATGTTTCGTTGGTTGCGGGACATCGAACTGGATGCTTTGGTTATGCA
CACCTCCGCAGGAGGGACATTCGCTCACTTTAGCAGTGAGCGCTGTCAGGCAGCCAGTTGTACGTTGGAGTTAGGCAAAG
CATTACCTTTTGGTGAGAATCAATTATCACAATTCAGTGCGATCACCCAAGGATTACGCTCCTTAGTCAGTGATAGTGCC
TTGCCTGCACGTAAGACAGAGAACATGAAGTACTACCGGGTCGTTAAATCACTGCTTAGGCAACACCCTGATTTTAAACT
ACGGGTGGCTGAGGATACGGTAAATTTCACACGCTTCGCACAAGGCACTCTACTTACAGAACAACCCAATGATAACTATC
GTGTTGAACACCCCTATGAGTGGATCTTATTTCCAAATCCACATGTTGCGTTGGGATTACGGGCTGGGATGATGTTGGTA
AAAATGTGTGAAAGTGAATTACCGATTACATGA

Upstream 100 bases:

>100_bases
CCTCAATCTGGGGCCGGTGTACCCGTTCCAGCGCAATTAGCCGGTGTGGCTTTAGCGAATAACCGAACGGCTCAATGATA
GAACCAATAGAGGAGCGATG

Downstream 100 bases:

>100_bases
GCTTGTTGGGGGCCAATACCGATGTGGGCGGTTCTCTGGAGTTATACCCATACAAATAGTAATAATTAATCTAATTTCAA
ATGAACTGTCTTGACGGTTC

Product: succinylglutamate desuccinylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MLDFLAITLSGKPPQVIQGETVNLKWQWLGEGILTLVPHRSYTQSVVISAGIHGNETAPIEILNQLVTDLLAGQLPLSVR
LLVLLGNPPAIRKGKRYLSNDINRMFGGRYQHYTPSDETRRASTLEQRVMAFFQASHTSERLHYDLHTAIRGSYHPRFGL
LPYQQTPYSAAMFRWLRDIELDALVMHTSAGGTFAHFSSERCQAASCTLELGKALPFGENQLSQFSAITQGLRSLVSDSA
LPARKTENMKYYRVVKSLLRQHPDFKLRVAEDTVNFTRFAQGTLLTEQPNDNYRVEHPYEWILFPNPHVALGLRAGMMLV
KMCESELPIT

Sequences:

>Translated_330_residues
MLDFLAITLSGKPPQVIQGETVNLKWQWLGEGILTLVPHRSYTQSVVISAGIHGNETAPIEILNQLVTDLLAGQLPLSVR
LLVLLGNPPAIRKGKRYLSNDINRMFGGRYQHYTPSDETRRASTLEQRVMAFFQASHTSERLHYDLHTAIRGSYHPRFGL
LPYQQTPYSAAMFRWLRDIELDALVMHTSAGGTFAHFSSERCQAASCTLELGKALPFGENQLSQFSAITQGLRSLVSDSA
LPARKTENMKYYRVVKSLLRQHPDFKLRVAEDTVNFTRFAQGTLLTEQPNDNYRVEHPYEWILFPNPHVALGLRAGMMLV
KMCESELPIT
>Mature_330_residues
MLDFLAITLSGKPPQVIQGETVNLKWQWLGEGILTLVPHRSYTQSVVISAGIHGNETAPIEILNQLVTDLLAGQLPLSVR
LLVLLGNPPAIRKGKRYLSNDINRMFGGRYQHYTPSDETRRASTLEQRVMAFFQASHTSERLHYDLHTAIRGSYHPRFGL
LPYQQTPYSAAMFRWLRDIELDALVMHTSAGGTFAHFSSERCQAASCTLELGKALPFGENQLSQFSAITQGLRSLVSDSA
LPARKTENMKYYRVVKSLLRQHPDFKLRVAEDTVNFTRFAQGTLLTEQPNDNYRVEHPYEWILFPNPHVALGLRAGMMLV
KMCESELPIT

Specific function: Transforms N(2)-succinylglutamate into succinate and glutamate

COG id: COG2988

COG function: function code E; Succinylglutamate desuccinylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily

Homologues:

Organism=Escherichia coli, GI1788040, Length=322, Percent_Identity=49.0683229813665, Blast_Score=325, Evalue=3e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASTE_YERP3 (A7FIL2)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001401090.1
- ProteinModelPortal:   A7FIL2
- SMR:   A7FIL2
- STRING:   A7FIL2
- GeneID:   5384842
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_2117
- eggNOG:   COG2988
- HOGENOM:   HBG298271
- OMA:   RWRWLGH
- ProtClustDB:   PRK05324
- BioCyc:   YPSE349747:YPSIP31758_2117-MONOMER
- HAMAP:   MF_00767
- InterPro:   IPR007036
- InterPro:   IPR016681
- PIRSF:   PIRSF017020
- TIGRFAMs:   TIGR03242

Pfam domain/function: PF04952 AstE_AspA

EC number: =3.5.1.96

Molecular weight: Translated: 37268; Mature: 37268

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: ACT_SITE 210-210

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDFLAITLSGKPPQVIQGETVNLKWQWLGEGILTLVPHRSYTQSVVISAGIHGNETAPI
CCEEEEEEECCCCCCEECCCEEEEEEEEECCCEEEEECCCCCCCEEEEEECCCCCCCCHH
EILNQLVTDLLAGQLPLSVRLLVLLGNPPAIRKGKRYLSNDINRMFGGRYQHYTPSDETR
HHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RASTLEQRVMAFFQASHTSERLHYDLHTAIRGSYHPRFGLLPYQQTPYSAAMFRWLRDIE
HHHHHHHHHHHHHHHCCCHHHHEEHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
LDALVMHTSAGGTFAHFSSERCQAASCTLELGKALPFGENQLSQFSAITQGLRSLVSDSA
CEEEEEEECCCCCEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
LPARKTENMKYYRVVKSLLRQHPDFKLRVAEDTVNFTRFAQGTLLTEQPNDNYRVEHPYE
CCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCEEEECCCCCCEEECCCEE
WILFPNPHVALGLRAGMMLVKMCESELPIT
EEEECCCCEEEHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLDFLAITLSGKPPQVIQGETVNLKWQWLGEGILTLVPHRSYTQSVVISAGIHGNETAPI
CCEEEEEEECCCCCCEECCCEEEEEEEEECCCEEEEECCCCCCCEEEEEECCCCCCCCHH
EILNQLVTDLLAGQLPLSVRLLVLLGNPPAIRKGKRYLSNDINRMFGGRYQHYTPSDETR
HHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RASTLEQRVMAFFQASHTSERLHYDLHTAIRGSYHPRFGLLPYQQTPYSAAMFRWLRDIE
HHHHHHHHHHHHHHHCCCHHHHEEHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
LDALVMHTSAGGTFAHFSSERCQAASCTLELGKALPFGENQLSQFSAITQGLRSLVSDSA
CEEEEEEECCCCCEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
LPARKTENMKYYRVVKSLLRQHPDFKLRVAEDTVNFTRFAQGTLLTEQPNDNYRVEHPYE
CCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCEEEECCCCCCEEECCCEE
WILFPNPHVALGLRAGMMLVKMCESELPIT
EEEECCCCEEEHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA