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The map label for this gene is cobB

Identifier: 218928766

GI number: 218928766

Start: 1854618

End: 1855454

Strand: Direct

Name: cobB

Synonym: YPO1630

Alternate gene names: 218928766

Gene position: 1854618-1855454 (Clockwise)

Preceding gene: 218928765

Following gene: 218928767

Centisome position: 39.85

GC content: 48.15

Gene sequence:

>837_bases
ATGCGCATTCGCCATAGGCTGTGTCGGTTTCGTAAGAGTAAGCATGTGCGGCATCAACGTTTTCGCTCCCGTATTTTTCA
TCGGGATAGTGCAGTAGCAAAAGAGATGAAGAAACCCTTTGTCGTTGTGCTTACTGGTGCTGGGATTTCTGCTGAGTCGG
GTATCCGTACTTTCCGTGCGGACGATGGTTTATGGGAAGATCACCGGGTAGAAGATGTGGCGACACCTGAAGGGTATCGG
CGTGATCCTGAGCTCGTCCAACGTTTTTATAATGAACGTCGCCGTCAGTTGCAGCAGCCAGACATAGCGCCAAATGCTGC
GCATTTCGCTCTGGCTGATTTAGAAGCCGTGCTGGGTGATAACCTGGTATTGATCACTCAAAATATTGATAACTTGCATG
AGAGAGCCGGCAGTAAGCGCGTTATTCATATGCATGGTGAATTGCTAAAAGTTCGCTGTACTCAATCTGGTCAGGTACTG
GATTGGCAAGGCGATCTCAGTGCGGATGAACGTTGTCATTGTTGCCAATTTCCCTCGCCATTACGGCCGCATATTGTTTG
GTTTGGCGAAATGCCGATGGGTATGGATGACATTTATCAGGCGCTGGCTGAGGCTGATTTCTTTATTTCAATTGGCACTT
CAGGCCATGTTTATCCTGCTGCGGGGTTTGTTCATGAATCTCATCTACATGGTGCCCATACAGTAGAACTGAATCTTGAA
CCTAGTCAGGTAGAAAGCCAGTTTGATGAGAAGCACTATGGCCTCGCCAGTAAAGTGGTACCAGAATATATTCGCGAGTT
TCTGACCACATGCGGTGAGAATCGTCAGGGGGATTGA

Upstream 100 bases:

>100_bases
TGATTCCGGGGGCGTTCGTGGTGCAGCGTTTCTGCATTTGGCAGAAAAATAATCGTCACGATTAATAACAAGACAGATAA
AGAAAAAGAGGCAAAAGGTT

Downstream 100 bases:

>100_bases
TTTCCCCGTTGGGTGCCTCAACTCATTCAGATAACTTACGGATATACCCAAATTGATTAGTGTTGCAGGTCGTCAGTCAA
TCAACACGTCCCGGCGAACT

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MRIRHRLCRFRKSKHVRHQRFRSRIFHRDSAVAKEMKKPFVVVLTGAGISAESGIRTFRADDGLWEDHRVEDVATPEGYR
RDPELVQRFYNERRRQLQQPDIAPNAAHFALADLEAVLGDNLVLITQNIDNLHERAGSKRVIHMHGELLKVRCTQSGQVL
DWQGDLSADERCHCCQFPSPLRPHIVWFGEMPMGMDDIYQALAEADFFISIGTSGHVYPAAGFVHESHLHGAHTVELNLE
PSQVESQFDEKHYGLASKVVPEYIREFLTTCGENRQGD

Sequences:

>Translated_278_residues
MRIRHRLCRFRKSKHVRHQRFRSRIFHRDSAVAKEMKKPFVVVLTGAGISAESGIRTFRADDGLWEDHRVEDVATPEGYR
RDPELVQRFYNERRRQLQQPDIAPNAAHFALADLEAVLGDNLVLITQNIDNLHERAGSKRVIHMHGELLKVRCTQSGQVL
DWQGDLSADERCHCCQFPSPLRPHIVWFGEMPMGMDDIYQALAEADFFISIGTSGHVYPAAGFVHESHLHGAHTVELNLE
PSQVESQFDEKHYGLASKVVPEYIREFLTTCGENRQGD
>Mature_278_residues
MRIRHRLCRFRKSKHVRHQRFRSRIFHRDSAVAKEMKKPFVVVLTGAGISAESGIRTFRADDGLWEDHRVEDVATPEGYR
RDPELVQRFYNERRRQLQQPDIAPNAAHFALADLEAVLGDNLVLITQNIDNLHERAGSKRVIHMHGELLKVRCTQSGQVL
DWQGDLSADERCHCCQFPSPLRPHIVWFGEMPMGMDDIYQALAEADFFISIGTSGHVYPAAGFVHESHLHGAHTVELNLE
PSQVESQFDEKHYGLASKVVPEYIREFLTTCGENRQGD

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain

Homologues:

Organism=Homo sapiens, GI300795542, Length=247, Percent_Identity=36.0323886639676, Blast_Score=154, Evalue=8e-38,
Organism=Homo sapiens, GI6912664, Length=304, Percent_Identity=33.2236842105263, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI13787215, Length=236, Percent_Identity=36.864406779661, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI6912662, Length=240, Percent_Identity=30.4166666666667, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI7657575, Length=229, Percent_Identity=32.7510917030568, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI308199517, Length=233, Percent_Identity=76.824034334764, Blast_Score=393, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17541892, Length=203, Percent_Identity=33.4975369458128, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71990482, Length=246, Percent_Identity=29.2682926829268, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17567771, Length=245, Percent_Identity=26.9387755102041, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71990487, Length=250, Percent_Identity=29.2, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI28571445, Length=122, Percent_Identity=37.7049180327869, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NPD_YERPE (Q8ZFR1)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AI0198
- RefSeq:   NP_669107.1
- RefSeq:   NP_993110.1
- RefSeq:   YP_002346641.1
- ProteinModelPortal:   Q8ZFR1
- SMR:   Q8ZFR1
- IntAct:   Q8ZFR1
- GeneID:   1146737
- GeneID:   1174469
- GeneID:   2765807
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO1630
- KEGG:   ypk:y1790
- KEGG:   ypm:YP_1760
- HOGENOM:   HBG641281
- OMA:   FVHEARL
- ProtClustDB:   PRK00481
- BioCyc:   YPES187410:Y1790-MONOMER
- BioCyc:   YPES214092:YPO1630-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01121
- InterPro:   IPR003000
- PANTHER:   PTHR11085

Pfam domain/function: PF02146 SIR2

EC number: 3.5.1.- [C]

Molecular weight: Translated: 31845; Mature: 31845

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS50305 SIRTUIN

Important sites: ACT_SITE 145-145

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIRHRLCRFRKSKHVRHQRFRSRIFHRDSAVAKEMKKPFVVVLTGAGISAESGIRTFRA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEC
DDGLWEDHRVEDVATPEGYRRDPELVQRFYNERRRQLQQPDIAPNAAHFALADLEAVLGD
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
NLVLITQNIDNLHERAGSKRVIHMHGELLKVRCTQSGQVLDWQGDLSADERCHCCQFPSP
CEEEEECCHHHHHHHCCCCEEEEECCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCC
LRPHIVWFGEMPMGMDDIYQALAEADFFISIGTSGHVYPAAGFVHESHLHGAHTVELNLE
CCCCEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCEECCCCCEECCCCCCEEEEEEECC
PSQVESQFDEKHYGLASKVVPEYIREFLTTCGENRQGD
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRIRHRLCRFRKSKHVRHQRFRSRIFHRDSAVAKEMKKPFVVVLTGAGISAESGIRTFRA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEC
DDGLWEDHRVEDVATPEGYRRDPELVQRFYNERRRQLQQPDIAPNAAHFALADLEAVLGD
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
NLVLITQNIDNLHERAGSKRVIHMHGELLKVRCTQSGQVLDWQGDLSADERCHCCQFPSP
CEEEEECCHHHHHHHCCCCEEEEECCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCC
LRPHIVWFGEMPMGMDDIYQALAEADFFISIGTSGHVYPAAGFVHESHLHGAHTVELNLE
CCCCEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCEECCCCCEECCCCCCEEEEEEECC
PSQVESQFDEKHYGLASKVVPEYIREFLTTCGENRQGD
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430