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The map label for this gene is pksI [H]

Identifier: 218928602

GI number: 218928602

Start: 1652093

End: 1652854

Strand: Direct

Name: pksI [H]

Synonym: YPO1459

Alternate gene names: 218928602

Gene position: 1652093-1652854 (Clockwise)

Preceding gene: 218928601

Following gene: 218928603

Centisome position: 35.5

GC content: 39.5

Gene sequence:

>762_bases
ATGACTAATATGAAAAAAATTTATTTAAGAAAAAGTGATGAAGGTATTGCTTTTATGCATATTAACGATCCAGTCAACAA
AAATGCCATTGATGATGAGTTTTGTTTACAGTTACATGGTATTGTCGACACGATAAAGCAAGACAAAACCATCAAAGTGC
TTATTTTGGTTGGATTGCCTGATATATTTTGTACCGGTGGAACCGAAGCGTTTTTACAAAGCCTAATCACACACTACAAA
ACTAATATTGAACACGAGTATGCCAATCACCTCAAACATTTTCTGAATATACCGATCCCGGTTATTGCGGCGATGGAAGG
CAGTGCTACAGGGGGGGGATTAGTCTTAGGTTTATATGCAGATATTTTAGTCGCGGCGGAAGAAAGTCGCTATGGCATGT
CATTTATGAACATGGGTTTCACCCCTGGGATGGGTTCAACTGCCATGAGTAAGGAGGCGTTTGGTTATTACACCGGTTTT
GAGATGATGGTCACCGGTAACTATATTAGGGGCAGTGAGCTGAAAGGAAAATGTTGTTTCAACTATATTTTGCCCCGCGC
CATGGTCATGGAAAAGGCCATTGAACTGGCAACCTCTATTGCTGAAAAACCTCGCCATAGTTTGGAATTATTAAAAAAAT
ACATGTCAATCGACCGGCGTAAAATGCTAGAGGAAACAAGCACTATCGAGGCGTTTATGCACCATATTGCATTTAATCAA
AGCAATATCGAACAACAAATACAAGATAATTACACGGGTTAG

Upstream 100 bases:

>100_bases
GGTAGCCATCTTCTGGTGGAGAGACTAAATGATGCTCAGACCATAGAAAAGATACGTCACTACTTAGAAGACATTAAGTT
ATTTAATGATGAATATGCGG

Downstream 100 bases:

>100_bases
ATATGCTGAATAACTTGAGTGAAATGACCGTATTGGTAACTGGTGGCACAAAAGGTATAGGCCGAGCCACTGTTGAAAGC
TTTGTGAAGGCGGGAGCAAA

Product: polyketide biosynthesis enoyl-CoA hydratase

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTNMKKIYLRKSDEGIAFMHINDPVNKNAIDDEFCLQLHGIVDTIKQDKTIKVLILVGLPDIFCTGGTEAFLQSLITHYK
TNIEHEYANHLKHFLNIPIPVIAAMEGSATGGGLVLGLYADILVAAEESRYGMSFMNMGFTPGMGSTAMSKEAFGYYTGF
EMMVTGNYIRGSELKGKCCFNYILPRAMVMEKAIELATSIAEKPRHSLELLKKYMSIDRRKMLEETSTIEAFMHHIAFNQ
SNIEQQIQDNYTG

Sequences:

>Translated_253_residues
MTNMKKIYLRKSDEGIAFMHINDPVNKNAIDDEFCLQLHGIVDTIKQDKTIKVLILVGLPDIFCTGGTEAFLQSLITHYK
TNIEHEYANHLKHFLNIPIPVIAAMEGSATGGGLVLGLYADILVAAEESRYGMSFMNMGFTPGMGSTAMSKEAFGYYTGF
EMMVTGNYIRGSELKGKCCFNYILPRAMVMEKAIELATSIAEKPRHSLELLKKYMSIDRRKMLEETSTIEAFMHHIAFNQ
SNIEQQIQDNYTG
>Mature_252_residues
TNMKKIYLRKSDEGIAFMHINDPVNKNAIDDEFCLQLHGIVDTIKQDKTIKVLILVGLPDIFCTGGTEAFLQSLITHYKT
NIEHEYANHLKHFLNIPIPVIAAMEGSATGGGLVLGLYADILVAAEESRYGMSFMNMGFTPGMGSTAMSKEAFGYYTGFE
MMVTGNYIRGSELKGKCCFNYILPRAMVMEKAIELATSIAEKPRHSLELLKKYMSIDRRKMLEETSTIEAFMHHIAFNQS
NIEQQIQDNYTG

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. May have a role in the decarboxylation of the (S)-3-methylglutaryl group attached to pksL [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=229, Percent_Identity=25.764192139738, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI4502327, Length=178, Percent_Identity=28.0898876404494, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=194, Percent_Identity=26.2886597938144, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788682, Length=200, Percent_Identity=28.5, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787660, Length=248, Percent_Identity=22.5806451612903, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=201, Percent_Identity=27.8606965174129, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=236, Percent_Identity=25.4237288135593, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25145438, Length=203, Percent_Identity=25.615763546798, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=202, Percent_Identity=25.7425742574257, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24653139, Length=214, Percent_Identity=26.1682242990654, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI20129971, Length=243, Percent_Identity=24.2798353909465, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24653477, Length=243, Percent_Identity=24.2798353909465, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24650670, Length=239, Percent_Identity=25.1046025104602, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 28416; Mature: 28285

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNMKKIYLRKSDEGIAFMHINDPVNKNAIDDEFCLQLHGIVDTIKQDKTIKVLILVGLP
CCCCEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCC
DIFCTGGTEAFLQSLITHYKTNIEHEYANHLKHFLNIPIPVIAAMEGSATGGGLVLGLYA
CEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHEEEECCCCCCCHHHHHHHH
DILVAAEESRYGMSFMNMGFTPGMGSTAMSKEAFGYYTGFEMMVTGNYIRGSELKGKCCF
HHHHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHCHHCCCEEEEEECCEEECCCCCCHHHH
NYILPRAMVMEKAIELATSIAEKPRHSLELLKKYMSIDRRKMLEETSTIEAFMHHIAFNQ
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SNIEQQIQDNYTG
HHHHHHHHCCCCC
>Mature Secondary Structure 
TNMKKIYLRKSDEGIAFMHINDPVNKNAIDDEFCLQLHGIVDTIKQDKTIKVLILVGLP
CCCEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCC
DIFCTGGTEAFLQSLITHYKTNIEHEYANHLKHFLNIPIPVIAAMEGSATGGGLVLGLYA
CEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHEEEECCCCCCCHHHHHHHH
DILVAAEESRYGMSFMNMGFTPGMGSTAMSKEAFGYYTGFEMMVTGNYIRGSELKGKCCF
HHHHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHCHHCCCEEEEEECCEEECCCCCCHHHH
NYILPRAMVMEKAIELATSIAEKPRHSLELLKKYMSIDRRKMLEETSTIEAFMHHIAFNQ
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SNIEQQIQDNYTG
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]