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The map label for this gene is helD [H]

Identifier: 218928586

GI number: 218928586

Start: 1632891

End: 1634876

Strand: Direct

Name: helD [H]

Synonym: YPO1440

Alternate gene names: 218928586

Gene position: 1632891-1634876 (Clockwise)

Preceding gene: 218928583

Following gene: 218928589

Centisome position: 35.09

GC content: 48.09

Gene sequence:

>1986_bases
ATGGAACTTAAAGCGACATCTTTTGGCAAACATCTGTCTCAGCATCCCTATAACCGGGTCCGCTTGCTGAATGCAGGCAT
CGAAGTCTGCGGTGATAAACATCAATACCTAATCCCTTTTAACCAACTGGTTAGTATTCAGTGTAAACGAGGTATCGTTT
GGGGCGAGCTTGAATTTGAGTTGCCGCAGCAGAAAGTTGTACGGTTGCACGGTACTGAATGGCAGGAAACACAACATTTC
TATCATTACTTGCTAGGGGTATGGCAGCAATGGAGTGAAGAAATGAGCCAGGTTTGTGCGGAGGTGCTGCATAAACAGGT
GGAGTCAATTAAGCGAATTGAACAGCAGGATAAATGGTTTAAACGCAGTGAATTAGGCCAGATACAAGTCATGATCCGCG
AGGCATTCAGTGCCTTACCAATGCCAGTGGAACGCTTAGCGGCGTTTGAATCCTGTCGAGCAGATTATGAATGCTGCCTG
CGTTGGTTACAGCAAGGTCGTCACAGTGTTGAACGATGTAATCAGCAATGGACAGCCCGTATGTTGGAGGAGTATCAAGA
TTTTTTCCAAAATGTAGAAACTTCGCCCCTGAATGCCTCACAAAGCCGGGCGGTGGTTAACGGTGAAGATTCAGTTTTGG
TGTTAGCCGGCGCGGGTAGCGGTAAAACATCGGTGCTGGTGGCGCGTGCGGGCTGGTTGCTACGTCGTAATGAGGCACTA
CCTGAGCAGATTTTATTGCTGGCATTTGGTCGCCAGGCGGCCGATGAAATGAATAGCCGTATTAAACAACGCTTGGGCGT
GGATAGCATCCAAGCTAAAACTTTTCATGCTCTGGCATTACATATTATCCAGCAAGGTAGCCGCAAGACACCGGTTATCA
GCAAGCTTGAGTCTGATAGCCAGGCCAGAAGGGCGTTGTTAATTCAACATTGGCAGCAACAGTGTAGCGAGAAGAAAGCA
CAGGACAAAAGGTTGGCTGCGAGGCTGGCAAGTCGCCTTGAACGTTGGGTAGGGTTGATGCGTATGTACGGTGGCAGTCA
GGCTGAAATGATTGAGCAAGCGAATGACGAGGTGCGTGACCTGTTTCAAAAGCGTATCCGTTTAATGGCACCGTTGCTAA
AAGCCTGGAAAACGGCATTGAAAGATGAGGGTGCCGTCGATTTTTCTGGGTTGATTCATCAGGCGGTTAACTTGCTGGAG
AAAGGGCGCTTTGTCAGCCCATGGAAGCATGTTTTGGTTGATGAGTTCCAGGATATCTCACCACAAAGGGCGATGTTACT
CGCGGCGCTGCGTAAACAGAATAAGCAAACTTGCCTGTTTGCGGTTGGCGATGATTGGCAGGCTATTTACCGCTTCAGTG
GTGCTGAACTTTCGCTGACAACGGCTTTTAGCGAGCATTTTGGTGAAGGTGCTGAATGTGCACTGGATACGACTTACCGC
TTTAATGATCGGATTGGTGATATTGCGAACCGGTTTATTCAGCAAAACCCTTATCAACTGAAGAAACCACTTAATAGCTT
GAGTAAGGGTAATAAAAAGTCGGTCATGATTTTACCCGATGAGCAGTTGGAGGCCCTATTAGATAAATTAAGCGGCTTTG
TTAAGGACGGCGAACGTATCCTAATATTGGCGCGTTACCATCATTTACGCCCCGAAGTTTTACAAAAAGCGGCGACGCGG
TGGCCAAAGTTGACTATCGATTTTATGACCATCCATGCCAGCAAAGGGCAGCAGGCTGATTATGTCATTATCGTTGGCTT
ACATGAGGGTAATGATGGGTTCCCAGCCGCGGCGCGGGAATCTGTACTGGAAGATGTGTTGCTACCTCCTCCCGAAGATT
TCCCTGATGCTGAAGAGCGGCGTTTGCTTTATGTCGCAATAACGCGTGCTAAGCACCAAGTGTGGTTACTACAAGATACG
GCACGGCCTTCTATTTTTGTCTCTCAACTCAGTGAATTAGGTGCCCTGGTACAACGTAAGCCCTAG

Upstream 100 bases:

>100_bases
TGTTACCAACTATCGGATACCTGTTGTTTAAACTCAATTATTTTATTTACACTCCATCATGTTACTTCAATTCACTTGAT
ATGGGTTATGGCGCGCAGAT

Downstream 100 bases:

>100_bases
CAAACATAATAAAGCGATGCTCAATACTAAACCCGGTGGTTGAACCACCGGGTGAGTTACCTATTTTAGGTCCAGGCGCT
GTTGCATGTAACGGTCATAA

Product: DNA helicase IV

Products: NA

Alternate protein names: 75 kDa helicase [H]

Number of amino acids: Translated: 661; Mature: 661

Protein sequence:

>661_residues
MELKATSFGKHLSQHPYNRVRLLNAGIEVCGDKHQYLIPFNQLVSIQCKRGIVWGELEFELPQQKVVRLHGTEWQETQHF
YHYLLGVWQQWSEEMSQVCAEVLHKQVESIKRIEQQDKWFKRSELGQIQVMIREAFSALPMPVERLAAFESCRADYECCL
RWLQQGRHSVERCNQQWTARMLEEYQDFFQNVETSPLNASQSRAVVNGEDSVLVLAGAGSGKTSVLVARAGWLLRRNEAL
PEQILLLAFGRQAADEMNSRIKQRLGVDSIQAKTFHALALHIIQQGSRKTPVISKLESDSQARRALLIQHWQQQCSEKKA
QDKRLAARLASRLERWVGLMRMYGGSQAEMIEQANDEVRDLFQKRIRLMAPLLKAWKTALKDEGAVDFSGLIHQAVNLLE
KGRFVSPWKHVLVDEFQDISPQRAMLLAALRKQNKQTCLFAVGDDWQAIYRFSGAELSLTTAFSEHFGEGAECALDTTYR
FNDRIGDIANRFIQQNPYQLKKPLNSLSKGNKKSVMILPDEQLEALLDKLSGFVKDGERILILARYHHLRPEVLQKAATR
WPKLTIDFMTIHASKGQQADYVIIVGLHEGNDGFPAAARESVLEDVLLPPPEDFPDAEERRLLYVAITRAKHQVWLLQDT
ARPSIFVSQLSELGALVQRKP

Sequences:

>Translated_661_residues
MELKATSFGKHLSQHPYNRVRLLNAGIEVCGDKHQYLIPFNQLVSIQCKRGIVWGELEFELPQQKVVRLHGTEWQETQHF
YHYLLGVWQQWSEEMSQVCAEVLHKQVESIKRIEQQDKWFKRSELGQIQVMIREAFSALPMPVERLAAFESCRADYECCL
RWLQQGRHSVERCNQQWTARMLEEYQDFFQNVETSPLNASQSRAVVNGEDSVLVLAGAGSGKTSVLVARAGWLLRRNEAL
PEQILLLAFGRQAADEMNSRIKQRLGVDSIQAKTFHALALHIIQQGSRKTPVISKLESDSQARRALLIQHWQQQCSEKKA
QDKRLAARLASRLERWVGLMRMYGGSQAEMIEQANDEVRDLFQKRIRLMAPLLKAWKTALKDEGAVDFSGLIHQAVNLLE
KGRFVSPWKHVLVDEFQDISPQRAMLLAALRKQNKQTCLFAVGDDWQAIYRFSGAELSLTTAFSEHFGEGAECALDTTYR
FNDRIGDIANRFIQQNPYQLKKPLNSLSKGNKKSVMILPDEQLEALLDKLSGFVKDGERILILARYHHLRPEVLQKAATR
WPKLTIDFMTIHASKGQQADYVIIVGLHEGNDGFPAAARESVLEDVLLPPPEDFPDAEERRLLYVAITRAKHQVWLLQDT
ARPSIFVSQLSELGALVQRKP
>Mature_661_residues
MELKATSFGKHLSQHPYNRVRLLNAGIEVCGDKHQYLIPFNQLVSIQCKRGIVWGELEFELPQQKVVRLHGTEWQETQHF
YHYLLGVWQQWSEEMSQVCAEVLHKQVESIKRIEQQDKWFKRSELGQIQVMIREAFSALPMPVERLAAFESCRADYECCL
RWLQQGRHSVERCNQQWTARMLEEYQDFFQNVETSPLNASQSRAVVNGEDSVLVLAGAGSGKTSVLVARAGWLLRRNEAL
PEQILLLAFGRQAADEMNSRIKQRLGVDSIQAKTFHALALHIIQQGSRKTPVISKLESDSQARRALLIQHWQQQCSEKKA
QDKRLAARLASRLERWVGLMRMYGGSQAEMIEQANDEVRDLFQKRIRLMAPLLKAWKTALKDEGAVDFSGLIHQAVNLLE
KGRFVSPWKHVLVDEFQDISPQRAMLLAALRKQNKQTCLFAVGDDWQAIYRFSGAELSLTTAFSEHFGEGAECALDTTYR
FNDRIGDIANRFIQQNPYQLKKPLNSLSKGNKKSVMILPDEQLEALLDKLSGFVKDGERILILARYHHLRPEVLQKAATR
WPKLTIDFMTIHASKGQQADYVIIVGLHEGNDGFPAAARESVLEDVLLPPPEDFPDAEERRLLYVAITRAKHQVWLLQDT
ARPSIFVSQLSELGALVQRKP

Specific function: Helicase IV catalyzes the unwinding of duplex DNA in the 3' to 5' direction with respect to the bound single strand in a reaction that is dependent upon the hydrolysis of ATP [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase ATP-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787196, Length=684, Percent_Identity=66.6666666666667, Blast_Score=940, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=318, Percent_Identity=29.559748427673, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI48994965, Length=338, Percent_Identity=28.4023668639053, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000212
- InterPro:   IPR014016
- InterPro:   IPR022161
- InterPro:   IPR003661 [H]

Pfam domain/function: PF12462 Nucleolin_N; PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 75620; Mature: 75620

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKATSFGKHLSQHPYNRVRLLNAGIEVCGDKHQYLIPFNQLVSIQCKRGIVWGELEFE
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHCCEEEEEEEEC
LPQQKVVRLHGTEWQETQHFYHYLLGVWQQWSEEMSQVCAEVLHKQVESIKRIEQQDKWF
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRSELGQIQVMIREAFSALPMPVERLAAFESCRADYECCLRWLQQGRHSVERCNQQWTAR
HHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MLEEYQDFFQNVETSPLNASQSRAVVNGEDSVLVLAGAGSGKTSVLVARAGWLLRRNEAL
HHHHHHHHHHCCCCCCCCCCCCCEEECCCCCEEEEEECCCCCCEEEEEHHCHHHHCCCCC
PEQILLLAFGRQAADEMNSRIKQRLGVDSIQAKTFHALALHIIQQGSRKTPVISKLESDS
HHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHH
QARRALLIQHWQQQCSEKKAQDKRLAARLASRLERWVGLMRMYGGSQAEMIEQANDEVRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHH
LFQKRIRLMAPLLKAWKTALKDEGAVDFSGLIHQAVNLLEKGRFVSPWKHVLVDEFQDIS
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
PQRAMLLAALRKQNKQTCLFAVGDDWQAIYRFSGAELSLTTAFSEHFGEGAECALDTTYR
HHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCEEEEHHHHHHCCCCCCEEEECCCH
FNDRIGDIANRFIQQNPYQLKKPLNSLSKGNKKSVMILPDEQLEALLDKLSGFVKDGERI
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCEE
LILARYHHLRPEVLQKAATRWPKLTIDFMTIHASKGQQADYVIIVGLHEGNDGFPAAARE
EEEEHHHHCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEEEEEECCCCCCCHHHHH
SVLEDVLLPPPEDFPDAEERRLLYVAITRAKHQVWLLQDTARPSIFVSQLSELGALVQRK
HHHHHHCCCCCCCCCCHHHCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCC
P
C
>Mature Secondary Structure
MELKATSFGKHLSQHPYNRVRLLNAGIEVCGDKHQYLIPFNQLVSIQCKRGIVWGELEFE
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHCCEEEEEEEEC
LPQQKVVRLHGTEWQETQHFYHYLLGVWQQWSEEMSQVCAEVLHKQVESIKRIEQQDKWF
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRSELGQIQVMIREAFSALPMPVERLAAFESCRADYECCLRWLQQGRHSVERCNQQWTAR
HHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MLEEYQDFFQNVETSPLNASQSRAVVNGEDSVLVLAGAGSGKTSVLVARAGWLLRRNEAL
HHHHHHHHHHCCCCCCCCCCCCCEEECCCCCEEEEEECCCCCCEEEEEHHCHHHHCCCCC
PEQILLLAFGRQAADEMNSRIKQRLGVDSIQAKTFHALALHIIQQGSRKTPVISKLESDS
HHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHH
QARRALLIQHWQQQCSEKKAQDKRLAARLASRLERWVGLMRMYGGSQAEMIEQANDEVRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHH
LFQKRIRLMAPLLKAWKTALKDEGAVDFSGLIHQAVNLLEKGRFVSPWKHVLVDEFQDIS
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
PQRAMLLAALRKQNKQTCLFAVGDDWQAIYRFSGAELSLTTAFSEHFGEGAECALDTTYR
HHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCEEEEHHHHHHCCCCCCEEEECCCH
FNDRIGDIANRFIQQNPYQLKKPLNSLSKGNKKSVMILPDEQLEALLDKLSGFVKDGERI
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCEE
LILARYHHLRPEVLQKAATRWPKLTIDFMTIHASKGQQADYVIIVGLHEGNDGFPAAARE
EEEEHHHHCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEEEEEECCCCCCCHHHHH
SVLEDVLLPPPEDFPDAEERRLLYVAITRAKHQVWLLQDTARPSIFVSQLSELGALVQRK
HHHHHHCCCCCCCCCCHHHCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2542273; 8905232; 9278503 [H]