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The map label for this gene is rumB [H]

Identifier: 218928486

GI number: 218928486

Start: 1501209

End: 1502339

Strand: Direct

Name: rumB [H]

Synonym: YPO1336

Alternate gene names: 218928486

Gene position: 1501209-1502339 (Clockwise)

Preceding gene: 218928485

Following gene: 218928487

Centisome position: 32.26

GC content: 52.17

Gene sequence:

>1131_bases
ATGCATTGCGCACAGTATACGGCAGGTCGCTGCCGTTCTTGTCAGTGGTTGGATAAACCCTATCCACAACAACTCGCTGA
TAAACAGCATCATCTGGAAAGCCTGTTGGCTGGTCATGCGGTCACCCAGTGGCTAGCACCGGTTTTTGGGCGCGAGAGTG
CCTTTCGTAACAAGGCGAAAATGGTCGTCAGCGGCAGTGTGGAACGCCCGTTACTGGGTATGCTGCATCGTGACGGTACA
CCGGTTGATTTATGCGCATGTCCACTTTATCCGCCCAGCTTCGAACCGGTATTTACGGTACTGAAAACCTTTATTGCCAG
GGCGGGTTTGACTCCCTATAACGTTGCTCGCAAGCGTGGCGAACTTAAATTCCTGTTACTCACGGAAAGTACCTACAACG
GTGAATTGATGCTGCGCTTTGTGCTGCGTTCTGAAACTAAATTAGCGCAGTTAATTGCTGCGTTGCCGTGGCTGCAACAA
CAGTTGCCGCAGTTGGCGGTGATCTCGGCTAATATTCAGCCAGTGCATATGGCCATTCTGGAAGGGGAGCGGGAGATCCC
GCTGACCGAACAACAGGCCCTGCCTGAGCGGTTTAATCAGGTGCCGTTGTATATCCGCCCACAAAGTTTTTTCCAGACCA
ATCCACAGGTGGCGGCTTCGTTGTATGCAACAGCACGGCAGTGGGTGCAGGAGCATGAGGTTCACAGTATGTGGGATCTG
TTCTGTGGTGTGGGCGGCTTTGGTTTACATTGTGCGGGGCCAGAGACTCAATTGACCGGCATTGAAATCAATGCTGAAGC
TATCGCCTGTGCCCGCCAGTCGGCTGAGCAGTTAGGGCTAAAAAATGTCAGTTTCGCCGCGCTGGATTCTACCCGCTTTG
CTACCGCTGAAGCCCAAATACCTGAACTGGTCTTGGTGAATCCACCACGGCGGGGGATCGGCCGCGAGTTATGTGATTAC
CTGAGCCAGATGGCACCTAAATTTATTCTCTATTCAAGTTGTAATGCAGAGACGATGGCGAAAGATATCAGTTTGCTTGC
GGGTTACCACATTGAACGGGTACAGCTGTTTGATATGTTCCCGCATACCAGTCACTACGAAGTGCTCACCTTGCTGACTC
TCCGTCGCTAG

Upstream 100 bases:

>100_bases
CAAAAATGAGTGTTTAACGCCATAGGGTGCTACAATCCGCGCCCATCGACAGCTTCGGCTGCACATTATCTACTGTCTCA
GAGTATAACGGTCAATATTA

Downstream 100 bases:

>100_bases
ACGTGGTTTGTGGCTTAAGTGAGTATGCAGTGAGCCAAAGTCAAAGCGTTAAAACGTTAAAGTCACTGGCGTTGCAGGTC
GGCAGCTAACAAGCGCATCC

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MHCAQYTAGRCRSCQWLDKPYPQQLADKQHHLESLLAGHAVTQWLAPVFGRESAFRNKAKMVVSGSVERPLLGMLHRDGT
PVDLCACPLYPPSFEPVFTVLKTFIARAGLTPYNVARKRGELKFLLLTESTYNGELMLRFVLRSETKLAQLIAALPWLQQ
QLPQLAVISANIQPVHMAILEGEREIPLTEQQALPERFNQVPLYIRPQSFFQTNPQVAASLYATARQWVQEHEVHSMWDL
FCGVGGFGLHCAGPETQLTGIEINAEAIACARQSAEQLGLKNVSFAALDSTRFATAEAQIPELVLVNPPRRGIGRELCDY
LSQMAPKFILYSSCNAETMAKDISLLAGYHIERVQLFDMFPHTSHYEVLTLLTLRR

Sequences:

>Translated_376_residues
MHCAQYTAGRCRSCQWLDKPYPQQLADKQHHLESLLAGHAVTQWLAPVFGRESAFRNKAKMVVSGSVERPLLGMLHRDGT
PVDLCACPLYPPSFEPVFTVLKTFIARAGLTPYNVARKRGELKFLLLTESTYNGELMLRFVLRSETKLAQLIAALPWLQQ
QLPQLAVISANIQPVHMAILEGEREIPLTEQQALPERFNQVPLYIRPQSFFQTNPQVAASLYATARQWVQEHEVHSMWDL
FCGVGGFGLHCAGPETQLTGIEINAEAIACARQSAEQLGLKNVSFAALDSTRFATAEAQIPELVLVNPPRRGIGRELCDY
LSQMAPKFILYSSCNAETMAKDISLLAGYHIERVQLFDMFPHTSHYEVLTLLTLRR
>Mature_376_residues
MHCAQYTAGRCRSCQWLDKPYPQQLADKQHHLESLLAGHAVTQWLAPVFGRESAFRNKAKMVVSGSVERPLLGMLHRDGT
PVDLCACPLYPPSFEPVFTVLKTFIARAGLTPYNVARKRGELKFLLLTESTYNGELMLRFVLRSETKLAQLIAALPWLQQ
QLPQLAVISANIQPVHMAILEGEREIPLTEQQALPERFNQVPLYIRPQSFFQTNPQVAASLYATARQWVQEHEVHSMWDL
FCGVGGFGLHCAGPETQLTGIEINAEAIACARQSAEQLGLKNVSFAALDSTRFATAEAQIPELVLVNPPRRGIGRELCDY
LSQMAPKFILYSSCNAETMAKDISLLAGYHIERVQLFDMFPHTSHYEVLTLLTLRR

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=226, Percent_Identity=27.4336283185841, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI51173878, Length=226, Percent_Identity=27.4336283185841, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1787083, Length=373, Percent_Identity=74.5308310991957, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI1789148, Length=374, Percent_Identity=26.2032085561497, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790403, Length=156, Percent_Identity=30.7692307692308, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24666579, Length=414, Percent_Identity=25.1207729468599, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011825
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.189 [H]

Molecular weight: Translated: 42202; Mature: 42202

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHCAQYTAGRCRSCQWLDKPYPQQLADKQHHLESLLAGHAVTQWLAPVFGRESAFRNKAK
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHE
MVVSGSVERPLLGMLHRDGTPVDLCACPLYPPSFEPVFTVLKTFIARAGLTPYNVARKRG
EEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCC
ELKFLLLTESTYNGELMLRFVLRSETKLAQLIAALPWLQQQLPQLAVISANIQPVHMAIL
CEEEEEEECCCCCCHHEEHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEE
EGEREIPLTEQQALPERFNQVPLYIRPQSFFQTNPQVAASLYATARQWVQEHEVHSMWDL
CCCCCCCCCHHHHHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FCGVGGFGLHCAGPETQLTGIEINAEAIACARQSAEQLGLKNVSFAALDSTRFATAEAQI
HHCCCCCCEEECCCCCEEEEEEECHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCC
PELVLVNPPRRGIGRELCDYLSQMAPKFILYSSCNAETMAKDISLLAGYHIERVQLFDMF
CEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCHHEEEEECCC
PHTSHYEVLTLLTLRR
CCCCCHHHHHHHHHCC
>Mature Secondary Structure
MHCAQYTAGRCRSCQWLDKPYPQQLADKQHHLESLLAGHAVTQWLAPVFGRESAFRNKAK
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHE
MVVSGSVERPLLGMLHRDGTPVDLCACPLYPPSFEPVFTVLKTFIARAGLTPYNVARKRG
EEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCC
ELKFLLLTESTYNGELMLRFVLRSETKLAQLIAALPWLQQQLPQLAVISANIQPVHMAIL
CEEEEEEECCCCCCHHEEHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEE
EGEREIPLTEQQALPERFNQVPLYIRPQSFFQTNPQVAASLYATARQWVQEHEVHSMWDL
CCCCCCCCCHHHHHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FCGVGGFGLHCAGPETQLTGIEINAEAIACARQSAEQLGLKNVSFAALDSTRFATAEAQI
HHCCCCCCEEECCCCCEEEEEEECHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCC
PELVLVNPPRRGIGRELCDYLSQMAPKFILYSSCNAETMAKDISLLAGYHIERVQLFDMF
CEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCHHEEEEECCC
PHTSHYEVLTLLTLRR
CCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA