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The map label for this gene is betT [H]

Identifier: 218928332

GI number: 218928332

Start: 1315102

End: 1317123

Strand: Direct

Name: betT [H]

Synonym: YPO1168

Alternate gene names: 218928332

Gene position: 1315102-1317123 (Clockwise)

Preceding gene: 218928328

Following gene: 218928333

Centisome position: 28.26

GC content: 49.36

Gene sequence:

>2022_bases
ATGGTGACTTCAGACATCGACACAAAACCGCAAAAGGATACGTTAAATCCGGTGGTGTTCTTTACGTCCGCGGGGTTGAT
CCTCGCGTTCTGTCTTATGACTATTTTTTATACTGACCTCTCTAATCGCTGGATTGGTATTACCCTTAACTGGGTCTCTG
CCACTTTTGGTTGGTACTATCTGCTGGCGGCCACGCTGTATATCGTTTTTGTTATTTATATTGCCACTTCACGCTATGGC
TCAATCAAACTTGGCCCCGAGCAGTCAAAGCCCGAGTTCAGCCTGGTCAGTTGGGCGGCAATGCTGTTCGCCGCCGGTAT
CGGTATTGACCTGATGTTCTTCTCGGTAGCCGAACCCGTGACGCAATATATGATGCCACCAGAAGGCCAGGGCCAGACTC
TGGAAGCCGCGCGGCAGGCGATGGTCTGGACGCTGTTCCACTATGGGCTGACAGGTTGGTCAATGTATGCATTGATGGGG
ATTGCACTCGGTTACTTCAGCTATCGCTATAACCTGCCGTTGACCATCCGTTCCGCGCTTTACCCTATCTTTGGTAAGCG
GATCGACGGGCCAATCGGCCACAGTGTGGATATCGCCGCCGTTATCGGGACGATTTTCGGGATTGCCACCACATTGGGGA
TTGGGGTGGTGCAGCTCAATTACGGCCTCAATGTCCTGTTTCATATCCCGGAAAGTCTGGCGGTTCAGGCCGGGTTAATT
CTGTTGTCGGTGGTGATGGCGGCAGTATCGGTGACATCGGGTGTCAACAAAGGCATTCGGATCCTCTCTGAAGTGAATGT
TCTGCTGGCGTTAGGCCTGATCCTATTCCTGCTGAATGCGCTGGTGCTTAACGTGGGGGATTATATCAATCGCTTTTTGG
GCATGACGCTCAACAGTTTTGCTTTTGATCGCCCAACCCAATGGATGAATAGCTGGACGCTGTTTTTCTGGGCATGGTGG
GTTGCCTGGTCACCCTTTGTTGGTCTGTTTCTGGCGCGTATTTCCCGTGGCCGCACTATCCGCCAATTTGTGGTCGGTAC
GTTGATCATACCCTTTGTCTTCACCTTGCTTTGGCTGTCTATCTTCGGTAATAGCGCGTTGTACCAGATCCTGCATGGCA
ATATTGCTTTTGCTAATGAAGTGATGGAGTTTCCGGAACGCGGTTTCTATAGCCTGTTAGCACAGTACCCCGGTTTCACT
TTGAGTGCTTCGGTTGCCACGATCACCGGCTTGCTATTTTATGTCACGTCAGCGGATTCCGGTTCGCTAGTGCTGGGGAA
TTTCACCTCTCGTCTGGCCGATATTAATAATGATTCCCCCAACTGGTTACGTACTTTCTGGTCTATTACTATCGGGTTAC
TGACTTTGGGAATGTTGATGACCGATGGTGTCGCCGCCTTGCAAAACGCAACCGTCATTATGGGGTTACCGTTCAGTTTT
GTGATTTTCTTCGTGATGGTCGGCTTGTATAAATCGCTAAAAATTGAGGATTACCGCAAAGCCAGCTCGCAACAGACACC
AACGCCTACGCTGATTTCAGGCAATGAAGTACTAAATTGGAAACAACGTTTATCGCGAGTAATGAACTACCCAGGTACCA
CTTATACCGCGAAAATACTGGATACCATTTGCCGCCCAGCGATGGAGGAAGTTGCGCAGGAACTGGCCGTACGCGGTGTT
AAAGTTGAATTCAACGAACTGCCGCCGAAGGAAGATGAACAACTGAATCGTCTTAATCATCTGGAGTTGCTGGTACATCT
GGGTGATGAGCAGAACTTCGTCTACCAGATCTGGCCGCAACGTTATTCTGTGCCGGCATTCACCTACCGTGCACGCTCAG
GGAAATCACACTATTACCGCCTTGAAACTTATCTGCTTGAAGGAACGCAGGGGAATGATTTGATGGACTACAGCAAAGAA
CAGATTATCAATAACATTCTTGACCAGTATGAGCGCCATATGAACTTCCTACATATTCAAAGTGAAGCGCCGGGAAATAT
CATGACATTTCCCGATCAATAA

Upstream 100 bases:

>100_bases
ACTCAGCGGATCGCCTGCGAGCAGTCTCTTGATATGCGCTAAGGGGCGCTTACCCACCAAATGTGATGAGCCTTACTTTT
TTAAAAATGCAGGGGAAACG

Downstream 100 bases:

>100_bases
TCCGCAATAGTGGGGTCTCTTCATCTGGCGAGTCGATGAAGAGATCACCACCGCTTAATCAAAATCATCTGCCGCTTTTT
CTGGCTTAACTTTATTAATG

Product: choline transport protein BetT

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MVTSDIDTKPQKDTLNPVVFFTSAGLILAFCLMTIFYTDLSNRWIGITLNWVSATFGWYYLLAATLYIVFVIYIATSRYG
SIKLGPEQSKPEFSLVSWAAMLFAAGIGIDLMFFSVAEPVTQYMMPPEGQGQTLEAARQAMVWTLFHYGLTGWSMYALMG
IALGYFSYRYNLPLTIRSALYPIFGKRIDGPIGHSVDIAAVIGTIFGIATTLGIGVVQLNYGLNVLFHIPESLAVQAGLI
LLSVVMAAVSVTSGVNKGIRILSEVNVLLALGLILFLLNALVLNVGDYINRFLGMTLNSFAFDRPTQWMNSWTLFFWAWW
VAWSPFVGLFLARISRGRTIRQFVVGTLIIPFVFTLLWLSIFGNSALYQILHGNIAFANEVMEFPERGFYSLLAQYPGFT
LSASVATITGLLFYVTSADSGSLVLGNFTSRLADINNDSPNWLRTFWSITIGLLTLGMLMTDGVAALQNATVIMGLPFSF
VIFFVMVGLYKSLKIEDYRKASSQQTPTPTLISGNEVLNWKQRLSRVMNYPGTTYTAKILDTICRPAMEEVAQELAVRGV
KVEFNELPPKEDEQLNRLNHLELLVHLGDEQNFVYQIWPQRYSVPAFTYRARSGKSHYYRLETYLLEGTQGNDLMDYSKE
QIINNILDQYERHMNFLHIQSEAPGNIMTFPDQ

Sequences:

>Translated_673_residues
MVTSDIDTKPQKDTLNPVVFFTSAGLILAFCLMTIFYTDLSNRWIGITLNWVSATFGWYYLLAATLYIVFVIYIATSRYG
SIKLGPEQSKPEFSLVSWAAMLFAAGIGIDLMFFSVAEPVTQYMMPPEGQGQTLEAARQAMVWTLFHYGLTGWSMYALMG
IALGYFSYRYNLPLTIRSALYPIFGKRIDGPIGHSVDIAAVIGTIFGIATTLGIGVVQLNYGLNVLFHIPESLAVQAGLI
LLSVVMAAVSVTSGVNKGIRILSEVNVLLALGLILFLLNALVLNVGDYINRFLGMTLNSFAFDRPTQWMNSWTLFFWAWW
VAWSPFVGLFLARISRGRTIRQFVVGTLIIPFVFTLLWLSIFGNSALYQILHGNIAFANEVMEFPERGFYSLLAQYPGFT
LSASVATITGLLFYVTSADSGSLVLGNFTSRLADINNDSPNWLRTFWSITIGLLTLGMLMTDGVAALQNATVIMGLPFSF
VIFFVMVGLYKSLKIEDYRKASSQQTPTPTLISGNEVLNWKQRLSRVMNYPGTTYTAKILDTICRPAMEEVAQELAVRGV
KVEFNELPPKEDEQLNRLNHLELLVHLGDEQNFVYQIWPQRYSVPAFTYRARSGKSHYYRLETYLLEGTQGNDLMDYSKE
QIINNILDQYERHMNFLHIQSEAPGNIMTFPDQ
>Mature_673_residues
MVTSDIDTKPQKDTLNPVVFFTSAGLILAFCLMTIFYTDLSNRWIGITLNWVSATFGWYYLLAATLYIVFVIYIATSRYG
SIKLGPEQSKPEFSLVSWAAMLFAAGIGIDLMFFSVAEPVTQYMMPPEGQGQTLEAARQAMVWTLFHYGLTGWSMYALMG
IALGYFSYRYNLPLTIRSALYPIFGKRIDGPIGHSVDIAAVIGTIFGIATTLGIGVVQLNYGLNVLFHIPESLAVQAGLI
LLSVVMAAVSVTSGVNKGIRILSEVNVLLALGLILFLLNALVLNVGDYINRFLGMTLNSFAFDRPTQWMNSWTLFFWAWW
VAWSPFVGLFLARISRGRTIRQFVVGTLIIPFVFTLLWLSIFGNSALYQILHGNIAFANEVMEFPERGFYSLLAQYPGFT
LSASVATITGLLFYVTSADSGSLVLGNFTSRLADINNDSPNWLRTFWSITIGLLTLGMLMTDGVAALQNATVIMGLPFSF
VIFFVMVGLYKSLKIEDYRKASSQQTPTPTLISGNEVLNWKQRLSRVMNYPGTTYTAKILDTICRPAMEEVAQELAVRGV
KVEFNELPPKEDEQLNRLNHLELLVHLGDEQNFVYQIWPQRYSVPAFTYRARSGKSHYYRLETYLLEGTQGNDLMDYSKE
QIINNILDQYERHMNFLHIQSEAPGNIMTFPDQ

Specific function: High-affinity uptake of choline driven by a proton- motive force [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=678, Percent_Identity=77.5811209439528, Blast_Score=1100, Evalue=0.0,
Organism=Escherichia coli, GI1788102, Length=457, Percent_Identity=28.6652078774617, Blast_Score=178, Evalue=9e-46,
Organism=Escherichia coli, GI1786224, Length=506, Percent_Identity=27.4703557312253, Blast_Score=164, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 75568; Mature: 75568

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTSDIDTKPQKDTLNPVVFFTSAGLILAFCLMTIFYTDLSNRWIGITLNWVSATFGWYY
CCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHH
LLAATLYIVFVIYIATSRYGSIKLGPEQSKPEFSLVSWAAMLFAAGIGIDLMFFSVAEPV
HHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TQYMMPPEGQGQTLEAARQAMVWTLFHYGLTGWSMYALMGIALGYFSYRYNLPLTIRSAL
HHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCEEHHHHH
YPIFGKRIDGPIGHSVDIAAVIGTIFGIATTLGIGVVQLNYGLNVLFHIPESLAVQAGLI
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCHHHHHHHHHH
LLSVVMAAVSVTSGVNKGIRILSEVNVLLALGLILFLLNALVLNVGDYINRFLGMTLNSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
AFDRPTQWMNSWTLFFWAWWVAWSPFVGLFLARISRGRTIRQFVVGTLIIPFVFTLLWLS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
IFGNSALYQILHGNIAFANEVMEFPERGFYSLLAQYPGFTLSASVATITGLLFYVTSADS
HHCCHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHEEECCCC
GSLVLGNFTSRLADINNDSPNWLRTFWSITIGLLTLGMLMTDGVAALQNATVIMGLPFSF
CCEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCHHH
VIFFVMVGLYKSLKIEDYRKASSQQTPTPTLISGNEVLNWKQRLSRVMNYPGTTYTAKIL
HHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCCCHHHHHH
DTICRPAMEEVAQELAVRGVKVEFNELPPKEDEQLNRLNHLELLVHLGDEQNFVYQIWPQ
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHEEEECCCCCEEEEECCC
RYSVPAFTYRARSGKSHYYRLETYLLEGTQGNDLMDYSKEQIINNILDQYERHMNFLHIQ
CCCCCCEEEECCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEE
SEAPGNIMTFPDQ
CCCCCCEEECCCC
>Mature Secondary Structure
MVTSDIDTKPQKDTLNPVVFFTSAGLILAFCLMTIFYTDLSNRWIGITLNWVSATFGWYY
CCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHH
LLAATLYIVFVIYIATSRYGSIKLGPEQSKPEFSLVSWAAMLFAAGIGIDLMFFSVAEPV
HHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TQYMMPPEGQGQTLEAARQAMVWTLFHYGLTGWSMYALMGIALGYFSYRYNLPLTIRSAL
HHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCEEHHHHH
YPIFGKRIDGPIGHSVDIAAVIGTIFGIATTLGIGVVQLNYGLNVLFHIPESLAVQAGLI
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCHHHHHHHHHH
LLSVVMAAVSVTSGVNKGIRILSEVNVLLALGLILFLLNALVLNVGDYINRFLGMTLNSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
AFDRPTQWMNSWTLFFWAWWVAWSPFVGLFLARISRGRTIRQFVVGTLIIPFVFTLLWLS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
IFGNSALYQILHGNIAFANEVMEFPERGFYSLLAQYPGFTLSASVATITGLLFYVTSADS
HHCCHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHEEECCCC
GSLVLGNFTSRLADINNDSPNWLRTFWSITIGLLTLGMLMTDGVAALQNATVIMGLPFSF
CCEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCHHH
VIFFVMVGLYKSLKIEDYRKASSQQTPTPTLISGNEVLNWKQRLSRVMNYPGTTYTAKIL
HHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCCCHHHHHH
DTICRPAMEEVAQELAVRGVKVEFNELPPKEDEQLNRLNHLELLVHLGDEQNFVYQIWPQ
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHEEEECCCCCEEEEECCC
RYSVPAFTYRARSGKSHYYRLETYLLEGTQGNDLMDYSKEQIINNILDQYERHMNFLHIQ
CCCCCCEEEECCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEE
SEAPGNIMTFPDQ
CCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]