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The map label for this gene is moaE

Identifier: 218928326

GI number: 218928326

Start: 1308655

End: 1309110

Strand: Direct

Name: moaE

Synonym: YPO1162

Alternate gene names: 218928326

Gene position: 1308655-1309110 (Clockwise)

Preceding gene: 218928325

Following gene: 218928327

Centisome position: 28.12

GC content: 54.17

Gene sequence:

>456_bases
ATGATGGAAAATACGCGTATCCGTGTCGGCGCAGAAGCGTTCAGTGTGGGTGATGAATACACATGGCTGTCGCAGTGCGA
TGAAGACGGCGCGGTGGTGACCTTCACTGGCAAAGTCCGTAATCATAATCTGGGGGCCAGTGTCAGTGCGCTAACACTGG
AACACTACCCCGGTATGACAGAAAAAGCGCTGACGGAGATTATTGCCGACGCACGCAGCCGTTGGTCATTGCAGCGGGTA
TCGGTCATCCATCGGGTCGGGCCACTATTCCCCGGAGATGAAATTGTCTTTGTGGGTGTGACCAGCGCTCATCGCAGTAT
GGCATTTGAAGCAGCAGAATTTATTATGGACTACCTGAAAACACGGGCACCATTCTGGAAGCGGGAAGCCACGGTCGAGG
GTGAGCGCTGGGTTGAATCACGGGACAGCGACCATATTGCGGCTAAACGCTGGTAA

Upstream 100 bases:

>100_bases
GCAAATTACTGACGGCGGTTAACCAATCATTAGTGAGTGCTCAGCATCCACTGGCCGCAGGTGATGAAGTGGCGTTTTTC
CCGCCGGTGACCGGGGGGTG

Downstream 100 bases:

>100_bases
TCTAGGCGCAGAACAGCATAAAAATCACGGCTAAATGTCTATTTTTACTCCTTTGTTTCCGCTATTTCTATGGTACCCTT
AAAAAGACGGTGGGCTGTCA

Product: molybdopterin guanine dinucleotide biosynthesis protein MoaE

Products: NA

Alternate protein names: MPT synthase subunit 2; Molybdenum cofactor biosynthesis protein E; Molybdopterin-converting factor large subunit; Molybdopterin-converting factor subunit 2

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MMENTRIRVGAEAFSVGDEYTWLSQCDEDGAVVTFTGKVRNHNLGASVSALTLEHYPGMTEKALTEIIADARSRWSLQRV
SVIHRVGPLFPGDEIVFVGVTSAHRSMAFEAAEFIMDYLKTRAPFWKREATVEGERWVESRDSDHIAAKRW

Sequences:

>Translated_151_residues
MMENTRIRVGAEAFSVGDEYTWLSQCDEDGAVVTFTGKVRNHNLGASVSALTLEHYPGMTEKALTEIIADARSRWSLQRV
SVIHRVGPLFPGDEIVFVGVTSAHRSMAFEAAEFIMDYLKTRAPFWKREATVEGERWVESRDSDHIAAKRW
>Mature_151_residues
MMENTRIRVGAEAFSVGDEYTWLSQCDEDGAVVTFTGKVRNHNLGASVSALTLEHYPGMTEKALTEIIADARSRWSLQRV
SVIHRVGPLFPGDEIVFVGVTSAHRSMAFEAAEFIMDYLKTRAPFWKREATVEGERWVESRDSDHIAAKRW

Specific function: Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur is provided by moaD

COG id: COG0314

COG function: function code H; Molybdopterin converting factor, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaE family

Homologues:

Organism=Homo sapiens, GI4758732, Length=131, Percent_Identity=34.3511450381679, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1787003, Length=150, Percent_Identity=77.3333333333333, Blast_Score=244, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI17509279, Length=107, Percent_Identity=33.6448598130841, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI21356259, Length=118, Percent_Identity=35.5932203389831, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAE_YERPE (Q8ZGW2)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AH0142
- RefSeq:   NP_670319.1
- RefSeq:   NP_992371.1
- RefSeq:   YP_002346201.1
- ProteinModelPortal:   Q8ZGW2
- SMR:   Q8ZGW2
- GeneID:   1147966
- GeneID:   1174011
- GeneID:   2764852
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO1162
- KEGG:   ypk:y3019
- KEGG:   ypm:YP_0997
- HOGENOM:   HBG460564
- OMA:   GAVACFV
- ProtClustDB:   PRK10678
- BioCyc:   YPES187410:Y3019-MONOMER
- BioCyc:   YPES214092:YPO1162-MONOMER
- InterPro:   IPR003448
- Gene3D:   G3DSA:3.90.1170.40
- PANTHER:   PTHR23404:SF2

Pfam domain/function: PF02391 MoaE; SSF54690 Mb_biosynth_MoaE

EC number: NA

Molecular weight: Translated: 17062; Mature: 17062

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMENTRIRVGAEAFSVGDEYTWLSQCDEDGAVVTFTGKVRNHNLGASVSALTLEHYPGMT
CCCCCEEEECHHHHHCCCCCHHHHHCCCCCCEEEEECCEECCCCCCCCEEEEHHHCCCCH
EKALTEIIADARSRWSLQRVSVIHRVGPLFPGDEIVFVGVTSAHRSMAFEAAEFIMDYLK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
TRAPFWKREATVEGERWVESRDSDHIAAKRW
HCCCCCCCCCCCCHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MMENTRIRVGAEAFSVGDEYTWLSQCDEDGAVVTFTGKVRNHNLGASVSALTLEHYPGMT
CCCCCEEEECHHHHHCCCCCHHHHHCCCCCCEEEEECCEECCCCCCCCEEEEHHHCCCCH
EKALTEIIADARSRWSLQRVSVIHRVGPLFPGDEIVFVGVTSAHRSMAFEAAEFIMDYLK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
TRAPFWKREATVEGERWVESRDSDHIAAKRW
HCCCCCCCCCCCCHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430