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The map label for this gene is thiP [H]

Identifier: 218927720

GI number: 218927720

Start: 564411

End: 565970

Strand: Reverse

Name: thiP [H]

Synonym: YPO0521

Alternate gene names: 218927720

Gene position: 565970-564411 (Counterclockwise)

Preceding gene: 218927721

Following gene: 218927719

Centisome position: 12.16

GC content: 54.04

Gene sequence:

>1560_bases
TTGCTGGCCGCTGGGCTGATTGTCGCGGTCGCCTTATTGGCGTTCGCCGCTATTTGGCACCATGCGCCCACAGCAGATTG
GCAAAGTGTCTGGCATGACCGTTATCTGTGGCATGTGATCCGCTTTACGTTCTGGCAGGCATTTTTATCCGCCCTGCTGT
CGGTGATACCTGCTATCTTTCTGGCGCGGGCGCTGTACCGCCGCAACTTCCCCGGCCGCCAGTTCATGCTGCGTTTATGT
GCCATGACGCTGGTTCTGCCGGTGTTAGTCGCGCTATTCGGCATTCTGACGGTCTATGGCCGTCAGGGGTGGCTGGCTCA
CCTGTTTGACTGGCTGGGTGTGGATTACCGCTTCTCCCCCTATGGCTTGCAAGGTATTTTGTTAGCGCATATCTTCTTTA
ACCTGCCACTGGCAACCCGCTTGTTGTTACAGTCGCTAGAAGGCATCGCCGTGGAACAACGCCAACTGGCCGCACAACTG
GGCATGAACGGCTGGCAGCATTTTCGCTGGGTAGAGTGGCCTTATCTGCGTCGTCAAATCCTGCCCACTGCCGCCCTGAT
TTTTATGTTGTGCTTTGCCAGCTTTGCCACCGTGCTGTCGCTGGGTGGCGGGCCACAGGCAACGACCATTGAACTGGCCA
TCTATCAGGCATTGAGCTACGACTATGATTTAGGCCGTGCCGCATTATTGGCGCTGATACAACTGGGCTGCTGCCTGGGT
TTAGTGGTACTCAGTCAGCGGCTGAACAGCGTGTTGACGGTGGGCAATACGCATGGGCAAATATGGCGTAATCCGCAAGA
CAGCGGCTGGGCGCGCCTTGGCGATACCCTGCTGATTTGTAGCGCATTACTCTTGATGGTACCGCCATTGCTAACAGTTG
TGATTGATGGCAGTAATAAAACAATACTGACCGTGTTACAACAGCCCGCACTCTGGCAAGCGTTCTTTACCTCAGTGACC
ATCGCTATTGGTGCCGGGCTATTGTGTGTCATCTTAACCATGATGTTGCTCTGGAGTAGCCGCGAACTGAAATTACGCCA
ACGCGCGGCTTACGGTCAGGCATTGGAGGTCAGCGGCATGGTTATTCTGGCGATGCCCGGCATTGTGTTGGCAACCGGTT
TCTTCTTGTTGCTGAATGATACGCTGGGATTGCCACAATCTCCTTATGGGTTGGTGATCCTCACCAATGCCCTGATGGCC
GTGCCCTACGCATTAAAAGTGCTGGATAACCCGATGCGTGATCTGGCTGAGCGCTACACACCGTTATGTTTATCACTGGA
TATCCGTGGCTGGCACCGTTTACGTTATATCGAGCTACGCGCCCTGAAACGCCCACTGGCTCAGGCGCTGGCCTTTGCCA
GTGTGTTATCTATCGGCGATTTTGGCGTAGTCGCGTTATTTGGTAATGAACACTTCCGCACCCTGCCATTTTATCTTTAT
CAACAGATAGGTTCCTACCGCAGCAATGATGGCGCAGTGACTGCCCTGTTGCTTTTGTTGCTCTGCTTCCTGCTGTTTAC
CCTTATTGAACGACTGCCGAGCCGCCATGCTAAAGCTTGA

Upstream 100 bases:

>100_bases
TGCAATTTGATGCTAAAGACGTGGCGGATAACCGCAGTAAATGGATTCAGGCATGGCAATCCGCCGTCAGCCGTTAATCG
CGCCCTGGCTATGGCCGGGG

Downstream 100 bases:

>100_bases
GAAAATCACCTATTTGTATGACCACTTACCCATGTGTTTTGATCTGCGCATTCAACCCGGTGAGCGGGTGGCGATCCTTG
GCCCAAGCGGCGCAGGTAAA

Product: thiamine transporter membrane protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MLAAGLIVAVALLAFAAIWHHAPTADWQSVWHDRYLWHVIRFTFWQAFLSALLSVIPAIFLARALYRRNFPGRQFMLRLC
AMTLVLPVLVALFGILTVYGRQGWLAHLFDWLGVDYRFSPYGLQGILLAHIFFNLPLATRLLLQSLEGIAVEQRQLAAQL
GMNGWQHFRWVEWPYLRRQILPTAALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDLGRAALLALIQLGCCLG
LVVLSQRLNSVLTVGNTHGQIWRNPQDSGWARLGDTLLICSALLLMVPPLLTVVIDGSNKTILTVLQQPALWQAFFTSVT
IAIGAGLLCVILTMMLLWSSRELKLRQRAAYGQALEVSGMVILAMPGIVLATGFFLLLNDTLGLPQSPYGLVILTNALMA
VPYALKVLDNPMRDLAERYTPLCLSLDIRGWHRLRYIELRALKRPLAQALAFASVLSIGDFGVVALFGNEHFRTLPFYLY
QQIGSYRSNDGAVTALLLLLLCFLLFTLIERLPSRHAKA

Sequences:

>Translated_519_residues
MLAAGLIVAVALLAFAAIWHHAPTADWQSVWHDRYLWHVIRFTFWQAFLSALLSVIPAIFLARALYRRNFPGRQFMLRLC
AMTLVLPVLVALFGILTVYGRQGWLAHLFDWLGVDYRFSPYGLQGILLAHIFFNLPLATRLLLQSLEGIAVEQRQLAAQL
GMNGWQHFRWVEWPYLRRQILPTAALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDLGRAALLALIQLGCCLG
LVVLSQRLNSVLTVGNTHGQIWRNPQDSGWARLGDTLLICSALLLMVPPLLTVVIDGSNKTILTVLQQPALWQAFFTSVT
IAIGAGLLCVILTMMLLWSSRELKLRQRAAYGQALEVSGMVILAMPGIVLATGFFLLLNDTLGLPQSPYGLVILTNALMA
VPYALKVLDNPMRDLAERYTPLCLSLDIRGWHRLRYIELRALKRPLAQALAFASVLSIGDFGVVALFGNEHFRTLPFYLY
QQIGSYRSNDGAVTALLLLLLCFLLFTLIERLPSRHAKA
>Mature_519_residues
MLAAGLIVAVALLAFAAIWHHAPTADWQSVWHDRYLWHVIRFTFWQAFLSALLSVIPAIFLARALYRRNFPGRQFMLRLC
AMTLVLPVLVALFGILTVYGRQGWLAHLFDWLGVDYRFSPYGLQGILLAHIFFNLPLATRLLLQSLEGIAVEQRQLAAQL
GMNGWQHFRWVEWPYLRRQILPTAALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDLGRAALLALIQLGCCLG
LVVLSQRLNSVLTVGNTHGQIWRNPQDSGWARLGDTLLICSALLLMVPPLLTVVIDGSNKTILTVLQQPALWQAFFTSVT
IAIGAGLLCVILTMMLLWSSRELKLRQRAAYGQALEVSGMVILAMPGIVLATGFFLLLNDTLGLPQSPYGLVILTNALMA
VPYALKVLDNPMRDLAERYTPLCLSLDIRGWHRLRYIELRALKRPLAQALAFASVLSIGDFGVVALFGNEHFRTLPFYLY
QQIGSYRSNDGAVTALLLLLLCFLLFTLIERLPSRHAKA

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=500, Percent_Identity=74.4, Blast_Score=698, Evalue=0.0,
Organism=Escherichia coli, GI87082099, Length=208, Percent_Identity=28.8461538461538, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 57893; Mature: 57893

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure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HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]