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The map label for this gene is priA [H]

Identifier: 218927324

GI number: 218927324

Start: 117531

End: 119729

Strand: Reverse

Name: priA [H]

Synonym: YPO0110

Alternate gene names: 218927324

Gene position: 119729-117531 (Counterclockwise)

Preceding gene: 218927326

Following gene: 218927323

Centisome position: 2.57

GC content: 52.89

Gene sequence:

>2199_bases
ATGTCAGTTGTTCAAGTGGCATTACCCGTACCGCTGGCCCGTACCTTCGATTACCGCTTAGACAGTGCCATGGCCTGCCC
TGTCGTAGGGGCCCGTGTCAGTGTGCCATTCGGTAAACGCAAAGCGATTGGTATCGTCGTGGGTCTCAGTGATACCAGCA
CCTTTCCGCTTGAACAACTCAAAACCATTGATGCCATTTTAGATAATCACAGCTTATTCCCCCCTAGCCTGTGGCGCATT
CTCTGCTGGGCAACCGAGTATTACCATTATCCGATAGGTGAAGTGCTGTTTCATGCACTGCCGATCTTGTTACGTCAGGG
GAGACCCGCCCAATCAGCGCCCTTATGGCAATGGTTTGTCACCGAGCAAGGCCGCGCTACACCACCAGAAAGCTTAAAAC
GCGCGCCCAAGCAGCAGCAAGCGCTTGCTGCATTATTGCAGAAACCGGTTTATCGCCATCAGGTTAATGAAATGGCATTA
ACTGAAAGTGCATTACAGGCATTACGCAGTAAAGGGTTGATTGATCTACGGGCGCAGGAAGCCACAACCACCGACTGGCG
TCACAGTTTTTCGGTACTGGGGGAACGCCTACGGCTTAATACTGAACAGGCCACCGCCGTCGGGGCTATTCGTAGCGAAG
ATAACCAATTTGCCGCTTGGTTGCTGGCAGGTGTTACGGGTTCAGGTAAAACCGAAGTTTACCTGAGCGTGCTGGAAAAC
ATTCTAGCGCAGGGCCGTCAGGCCCTAATTCTGGTGCCTGAAATTGGCCTGACCCCACAAACCATCGCCCGTTTTCGTGA
ACGGTTTAACGCACCGGTTGAGGTTCTCCATTCAGGCTTGAACGACAGTGAGCGCTTATCCGTCTGGTTGCGGGCACGTA
GTGGTGAGGCGGCGATTGTGATTGGGACCCGCTCAGCCTTGTTTACGCCGTTTTCACGCCTTGGCGTCATCATTATTGAT
GAAGAGCATGACAGCTCTTATAAACAGCAGGAAGGTTGGCGTTATCATGCCAGGGATTTAGCGGTATTTCGCGCCCGCGA
AGAGGGTATTCCTATCGTCATGGGGACGGCAACTCCCGCGCTTGAGACTCTGCATAACGTCCAAATGGGTAAATACCGCC
AGCTAACCCTGACCAAACGCGCAGGGAGTGCCAAACCTGCGGCACAACATTTGCTGGATCTAAAAGGCCTGCCATTGAAA
GTCGGCCTCTCTCAACCCCTGCTGAAACAGATGAAAACGCACCTGCAAGCGGGTAATCAGGTGATTTTATTCCTCAACCG
CCGTGGCTATGCACCTGCCCTGTTGTGTCACGAATGTGGCTGGATTGCCGAATGTCAGCGCTGCGACCACTATTACACGC
TGCATCAAAACCACCGTCAGTTACGTTGCCACCACTGTGACAGCCAACGGCCTGTGCCTCAGCAATGCCCTAAATGCGGA
TCGACTCATCTGGTCTCCGTTGGCGTGGGGACGGAACAGTTGGAAAATGAACTGGCACCGTTATTTCCTGAGACACCAAT
AACCCGCATTGACCGCGATACGACCAGCCGTAAAGGATCGCTGGAGCAATATCTGGCGGATGTTCACCAAGGTGGTGCAC
GCATTTTGATTGGTACACAAATGTTGGCCAAAGGGCACCACTTCCCTGACGTCACCTTGGTGGCGCTACTGGATGTTGAT
GGCGCGCTGTTTTCAGCGGATTTCCGCTCAGCCGAGCGCTTTGCCCAGCTTTATACGCAGGTCTCAGGCCGGGCCGGGCG
GGCAGGCAAGCAAGGGGAAGTGATTTTACAAACTCACCACCCCGAGCATCCCCTGCTGCAAATTCTGTTACAGCAGGGCT
ACGATGCGTTTGCCAAACAAGCGCTAGAAGAGCGTAAAAGCGTGTTTTTACCGCCCTATACCAGCCATATTATCGTGCGC
AGTGAAGATCACGATAACCAACAATCAGCCCTCTTCCTGCAACAATTACGTAATTTGCTGGAGGCCAGCCCCCTCAAAGA
TGAGGCGTTATGGATTATGGGGCCAGTTCCGGCGTTACAAGCCAAACGGGGTGGGCGCTTTCGCTGGCAGTTATTGCTAC
AACATCCCTCACGGCAACTGCTGCAACGGCTGATTAAAACCTCTCAGCCATTAATCAGTACCTTACCTCAGGCACGCAAA
GTGAAATGGACAATAGATGTCGATCCTATCGATAGTTGA

Upstream 100 bases:

>100_bases
ACGAAACCGCGCAATCGAATCCGCACATTGTGAAGTATGCGGTGTAAACTGTTCAATCTTTTTTTATTCAGGCCAATCTC
TTTATTCAGGATGACATCAT

Downstream 100 bases:

>100_bases
TATCTATACCCAAAATCATTAACACGGTAGGTGAACAGCGAATAAATCCCGATGAACTGACACGAAAGTGATTCAGGTGA
GGGGATATGGCGAACCCCTT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 732; Mature: 731

Protein sequence:

>732_residues
MSVVQVALPVPLARTFDYRLDSAMACPVVGARVSVPFGKRKAIGIVVGLSDTSTFPLEQLKTIDAILDNHSLFPPSLWRI
LCWATEYYHYPIGEVLFHALPILLRQGRPAQSAPLWQWFVTEQGRATPPESLKRAPKQQQALAALLQKPVYRHQVNEMAL
TESALQALRSKGLIDLRAQEATTTDWRHSFSVLGERLRLNTEQATAVGAIRSEDNQFAAWLLAGVTGSGKTEVYLSVLEN
ILAQGRQALILVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSVWLRARSGEAAIVIGTRSALFTPFSRLGVIIID
EEHDSSYKQQEGWRYHARDLAVFRAREEGIPIVMGTATPALETLHNVQMGKYRQLTLTKRAGSAKPAAQHLLDLKGLPLK
VGLSQPLLKQMKTHLQAGNQVILFLNRRGYAPALLCHECGWIAECQRCDHYYTLHQNHRQLRCHHCDSQRPVPQQCPKCG
STHLVSVGVGTEQLENELAPLFPETPITRIDRDTTSRKGSLEQYLADVHQGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVILQTHHPEHPLLQILLQQGYDAFAKQALEERKSVFLPPYTSHIIVR
SEDHDNQQSALFLQQLRNLLEASPLKDEALWIMGPVPALQAKRGGRFRWQLLLQHPSRQLLQRLIKTSQPLISTLPQARK
VKWTIDVDPIDS

Sequences:

>Translated_732_residues
MSVVQVALPVPLARTFDYRLDSAMACPVVGARVSVPFGKRKAIGIVVGLSDTSTFPLEQLKTIDAILDNHSLFPPSLWRI
LCWATEYYHYPIGEVLFHALPILLRQGRPAQSAPLWQWFVTEQGRATPPESLKRAPKQQQALAALLQKPVYRHQVNEMAL
TESALQALRSKGLIDLRAQEATTTDWRHSFSVLGERLRLNTEQATAVGAIRSEDNQFAAWLLAGVTGSGKTEVYLSVLEN
ILAQGRQALILVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSVWLRARSGEAAIVIGTRSALFTPFSRLGVIIID
EEHDSSYKQQEGWRYHARDLAVFRAREEGIPIVMGTATPALETLHNVQMGKYRQLTLTKRAGSAKPAAQHLLDLKGLPLK
VGLSQPLLKQMKTHLQAGNQVILFLNRRGYAPALLCHECGWIAECQRCDHYYTLHQNHRQLRCHHCDSQRPVPQQCPKCG
STHLVSVGVGTEQLENELAPLFPETPITRIDRDTTSRKGSLEQYLADVHQGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVILQTHHPEHPLLQILLQQGYDAFAKQALEERKSVFLPPYTSHIIVR
SEDHDNQQSALFLQQLRNLLEASPLKDEALWIMGPVPALQAKRGGRFRWQLLLQHPSRQLLQRLIKTSQPLISTLPQARK
VKWTIDVDPIDS
>Mature_731_residues
SVVQVALPVPLARTFDYRLDSAMACPVVGARVSVPFGKRKAIGIVVGLSDTSTFPLEQLKTIDAILDNHSLFPPSLWRIL
CWATEYYHYPIGEVLFHALPILLRQGRPAQSAPLWQWFVTEQGRATPPESLKRAPKQQQALAALLQKPVYRHQVNEMALT
ESALQALRSKGLIDLRAQEATTTDWRHSFSVLGERLRLNTEQATAVGAIRSEDNQFAAWLLAGVTGSGKTEVYLSVLENI
LAQGRQALILVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSVWLRARSGEAAIVIGTRSALFTPFSRLGVIIIDE
EHDSSYKQQEGWRYHARDLAVFRAREEGIPIVMGTATPALETLHNVQMGKYRQLTLTKRAGSAKPAAQHLLDLKGLPLKV
GLSQPLLKQMKTHLQAGNQVILFLNRRGYAPALLCHECGWIAECQRCDHYYTLHQNHRQLRCHHCDSQRPVPQQCPKCGS
THLVSVGVGTEQLENELAPLFPETPITRIDRDTTSRKGSLEQYLADVHQGGARILIGTQMLAKGHHFPDVTLVALLDVDG
ALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVILQTHHPEHPLLQILLQQGYDAFAKQALEERKSVFLPPYTSHIIVRS
EDHDNQQSALFLQQLRNLLEASPLKDEALWIMGPVPALQAKRGGRFRWQLLLQHPSRQLLQRLIKTSQPLISTLPQARKV
KWTIDVDPIDS

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=733, Percent_Identity=72.987721691678, Blast_Score=1113, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=233, Percent_Identity=29.6137339055794, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81894; Mature: 81763

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVQVALPVPLARTFDYRLDSAMACPVVGARVSVPFGKRKAIGIVVGLSDTSTFPLEQL
CCEEEEECCCCHHHHHHHHCCCCCCCCEECCEEECCCCCCCEEEEEEECCCCCCCCHHHH
KTIDAILDNHSLFPPSLWRILCWATEYYHYPIGEVLFHALPILLRQGRPAQSAPLWQWFV
HHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
TEQGRATPPESLKRAPKQQQALAALLQKPVYRHQVNEMALTESALQALRSKGLIDLRAQE
CCCCCCCCHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
ATTTDWRHSFSVLGERLRLNTEQATAVGAIRSEDNQFAAWLLAGVTGSGKTEVYLSVLEN
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEHEEECCCCCCHHHHHHHHHH
ILAQGRQALILVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSVWLRARSGEAAIV
HHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEEECCCCEEEE
IGTRSALFTPFSRLGVIIIDEEHDSSYKQQEGWRYHARDLAVFRAREEGIPIVMGTATPA
EECCHHHHCCHHHCCEEEEECCCCCCHHHHCCCEEHHHHHHHHHHHCCCCCEEEECCCHH
LETLHNVQMGKYRQLTLTKRAGSAKPAAQHLLDLKGLPLKVGLSQPLLKQMKTHLQAGNQ
HHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCE
VILFLNRRGYAPALLCHECGWIAECQRCDHYYTLHQNHRQLRCHHCDSQRPVPQQCPKCG
EEEEEECCCCCCHHHHHHCCCHHHHHHCCCEEEECCCCCCEEEECCCCCCCCHHHCCCCC
STHLVSVGVGTEQLENELAPLFPETPITRIDRDTTSRKGSLEQYLADVHQGGARILIGTQ
CCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHH
MLAKGHHFPDVTLVALLDVDGALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVILQTHH
HHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCC
PEHPLLQILLQQGYDAFAKQALEERKSVFLPPYTSHIIVRSEDHDNQQSALFLQQLRNLL
CCCHHHHHHHHHCHHHHHHHHHHHHHCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
EASPLKDEALWIMGPVPALQAKRGGRFRWQLLLQHPSRQLLQRLIKTSQPLISTLPQARK
HCCCCCCCCEEEECCCCHHHHCCCCCEEEEEEHHCCHHHHHHHHHHCCCHHHHHCCCCCE
VKWTIDVDPIDS
EEEEEECCCCCC
>Mature Secondary Structure 
SVVQVALPVPLARTFDYRLDSAMACPVVGARVSVPFGKRKAIGIVVGLSDTSTFPLEQL
CEEEEECCCCHHHHHHHHCCCCCCCCEECCEEECCCCCCCEEEEEEECCCCCCCCHHHH
KTIDAILDNHSLFPPSLWRILCWATEYYHYPIGEVLFHALPILLRQGRPAQSAPLWQWFV
HHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
TEQGRATPPESLKRAPKQQQALAALLQKPVYRHQVNEMALTESALQALRSKGLIDLRAQE
CCCCCCCCHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
ATTTDWRHSFSVLGERLRLNTEQATAVGAIRSEDNQFAAWLLAGVTGSGKTEVYLSVLEN
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEHEEECCCCCCHHHHHHHHHH
ILAQGRQALILVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSVWLRARSGEAAIV
HHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEEECCCCEEEE
IGTRSALFTPFSRLGVIIIDEEHDSSYKQQEGWRYHARDLAVFRAREEGIPIVMGTATPA
EECCHHHHCCHHHCCEEEEECCCCCCHHHHCCCEEHHHHHHHHHHHCCCCCEEEECCCHH
LETLHNVQMGKYRQLTLTKRAGSAKPAAQHLLDLKGLPLKVGLSQPLLKQMKTHLQAGNQ
HHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCE
VILFLNRRGYAPALLCHECGWIAECQRCDHYYTLHQNHRQLRCHHCDSQRPVPQQCPKCG
EEEEEECCCCCCHHHHHHCCCHHHHHHCCCEEEECCCCCCEEEECCCCCCCCHHHCCCCC
STHLVSVGVGTEQLENELAPLFPETPITRIDRDTTSRKGSLEQYLADVHQGGARILIGTQ
CCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHH
MLAKGHHFPDVTLVALLDVDGALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVILQTHH
HHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCC
PEHPLLQILLQQGYDAFAKQALEERKSVFLPPYTSHIIVRSEDHDNQQSALFLQQLRNLL
CCCHHHHHHHHHCHHHHHHHHHHHHHCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
EASPLKDEALWIMGPVPALQAKRGGRFRWQLLLQHPSRQLLQRLIKTSQPLISTLPQARK
HCCCCCCCCEEEECCCCHHHHCCCCCEEEEEEHHCCHHHHHHHHHHCCCHHHHHCCCCCE
VKWTIDVDPIDS
EEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]