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The map label for this gene is ywrK [H]

Identifier: 218906401

GI number: 218906401

Start: 5009432

End: 5010790

Strand: Direct

Name: ywrK [H]

Synonym: BCAH820_5315

Alternate gene names: 218906401

Gene position: 5009432-5010790 (Clockwise)

Preceding gene: 218906400

Following gene: 218906404

Centisome position: 94.47

GC content: 36.72

Gene sequence:

>1359_bases
ATGAGCGTTGAAATATGGATTACTATTATTGTATTTTTCTTAACGATGATTGTTATCTTTTGGCGACCTCGTGGTTTAAA
TGAGGCGTGGCCGGCAGCAATTGGTGCTGGCATTATCCTGATTACTGGACTTGTATCAAAACCAGACGTCATGGACATTA
TTAGTAAAATTGGCGGTGCCTCCATAACAATATTAGCGACCATTGTTATGGCAGTTATATTAGAAAGCTTCGGCTTCTTC
CACTGGTCTGCGGCGAAACTTGCAAACTTAGCAAAAGGTTCCGGTCGCCGTTTATACTGGTATATTCAATTATTATGTTT
TCTTATGACTCTTTTATTTAATAACGACGGTAGTATTTTAATTACAACTCCAATTTTAATTCTTCTTCTAAAAAATCTTC
AATTAAAACCACATCAACAAATCCCTTATTTATTAAGCGGTGCTTTAATTGCTACTGCATCTAGTGCACCAATTGGTGTA
AGTAATATCGTAAACTTAATCGCATTAAATATCGTGAATATGACTCTTTATATGCATACTGCTATGATGTTTGTACCTGC
AACATTAGGGCTATTGTTTATGTCATGGCTTATGTACACAGTTTTAAAAAGAAAACTACCAGAAAGATTACCTGTTTCTT
CATATGATATCGAAGAAATATTCTTCACGAAAAACTTCCATCCGTTAAAAGGACAAAATTCTGTTGATACGAAACAAAAA
CGGACAAAATTCATGCTAAAAGTATTAGGCTTCGTCTTCCTTATGCGCTGTCTTCTATTCGTCGCATCCTTCTTATCTAT
CCCAATTGAAATTGTTGCCGTACTCGGCTCACTCGTTCTTCTCATCTGGAGATGGTACTACTTACGAACAAATCCTGTCG
ACATTTTGAAAAAAACACCGTGGCACATTTTAATTTTTGCTTTCTCTATGTACGTCATTATTTACGGACTTCACAACGCA
GGATTAACAACAGCACTTGTAAATTGGCTCGAACCAGTTGTAAGTCAGCATCTACTTTATGCTAGCTTTGCAATGGGCGG
ACTCGTTTCCCTCCTCTCCAACATCTTCAATAATCACCCTGCACTTATGATTGGTACCATTACATTAACTGAAATGGGAC
TAGACCCGATTACATTAAAAACGATCTATCTCGCAAACATTATTGGTAGTGACATCGGTTCACTATTATTACCAATCGGT
ACGCTAGCTTCCCTCATTTGGATGTATATCCTGAGACAAAACAAAATTAAAGTAAAATGGAAAGACTATTTAAGTGTATC
CCTTATCGTCATTCCGCTCACAACAGTCGTTACATTATTCCTCTTATACTACTGGGTACACTTATTCTTCGCCTTATAA

Upstream 100 bases:

>100_bases
TTATACGTCTAGTTCCTATTACTTTTTGCTGAATATACTACCATTATAAACTTGAGACAAGCTACATCTGTTCAAGGGAA
AATAGAAAGGTAGGCAAAAT

Downstream 100 bases:

>100_bases
ACGACAGAAGCCCACTTTGCTACCGTCTTAGCAAAGCGGGCTTCTTCTATATTTAAGTTAGGTACTACAGTGTGATCAAT
CAAGTAAAGAGGTGTAACGT

Product: putative arsenical pump family protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSVEIWITIIVFFLTMIVIFWRPRGLNEAWPAAIGAGIILITGLVSKPDVMDIISKIGGASITILATIVMAVILESFGFF
HWSAAKLANLAKGSGRRLYWYIQLLCFLMTLLFNNDGSILITTPILILLLKNLQLKPHQQIPYLLSGALIATASSAPIGV
SNIVNLIALNIVNMTLYMHTAMMFVPATLGLLFMSWLMYTVLKRKLPERLPVSSYDIEEIFFTKNFHPLKGQNSVDTKQK
RTKFMLKVLGFVFLMRCLLFVASFLSIPIEIVAVLGSLVLLIWRWYYLRTNPVDILKKTPWHILIFAFSMYVIIYGLHNA
GLTTALVNWLEPVVSQHLLYASFAMGGLVSLLSNIFNNHPALMIGTITLTEMGLDPITLKTIYLANIIGSDIGSLLLPIG
TLASLIWMYILRQNKIKVKWKDYLSVSLIVIPLTTVVTLFLLYYWVHLFFAL

Sequences:

>Translated_452_residues
MSVEIWITIIVFFLTMIVIFWRPRGLNEAWPAAIGAGIILITGLVSKPDVMDIISKIGGASITILATIVMAVILESFGFF
HWSAAKLANLAKGSGRRLYWYIQLLCFLMTLLFNNDGSILITTPILILLLKNLQLKPHQQIPYLLSGALIATASSAPIGV
SNIVNLIALNIVNMTLYMHTAMMFVPATLGLLFMSWLMYTVLKRKLPERLPVSSYDIEEIFFTKNFHPLKGQNSVDTKQK
RTKFMLKVLGFVFLMRCLLFVASFLSIPIEIVAVLGSLVLLIWRWYYLRTNPVDILKKTPWHILIFAFSMYVIIYGLHNA
GLTTALVNWLEPVVSQHLLYASFAMGGLVSLLSNIFNNHPALMIGTITLTEMGLDPITLKTIYLANIIGSDIGSLLLPIG
TLASLIWMYILRQNKIKVKWKDYLSVSLIVIPLTTVVTLFLLYYWVHLFFAL
>Mature_451_residues
SVEIWITIIVFFLTMIVIFWRPRGLNEAWPAAIGAGIILITGLVSKPDVMDIISKIGGASITILATIVMAVILESFGFFH
WSAAKLANLAKGSGRRLYWYIQLLCFLMTLLFNNDGSILITTPILILLLKNLQLKPHQQIPYLLSGALIATASSAPIGVS
NIVNLIALNIVNMTLYMHTAMMFVPATLGLLFMSWLMYTVLKRKLPERLPVSSYDIEEIFFTKNFHPLKGQNSVDTKQKR
TKFMLKVLGFVFLMRCLLFVASFLSIPIEIVAVLGSLVLLIWRWYYLRTNPVDILKKTPWHILIFAFSMYVIIYGLHNAG
LTTALVNWLEPVVSQHLLYASFAMGGLVSLLSNIFNNHPALMIGTITLTEMGLDPITLKTIYLANIIGSDIGSLLLPIGT
LASLIWMYILRQNKIKVKWKDYLSVSLIVIPLTTVVTLFLLYYWVHLFFAL

Specific function: Involved In Arsenical Resistance. Thought To Form The Channel Of An Arsenite Pump (By Similarity). [C]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=420, Percent_Identity=29.0476190476191, Blast_Score=199, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 50892; Mature: 50761

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEIWITIIVFFLTMIVIFWRPRGLNEAWPAAIGAGIILITGLVSKPDVMDIISKIGGA
CCEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCHHHHHHHCCCCCHHHHHHHHHCCC
SITILATIVMAVILESFGFFHWSAAKLANLAKGSGRRLYWYIQLLCFLMTLLFNNDGSIL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
ITTPILILLLKNLQLKPHQQIPYLLSGALIATASSAPIGVSNIVNLIALNIVNMTLYMHT
EHHHHHHHHHHHCCCCCCCCCCHHHHCHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
AMMFVPATLGLLFMSWLMYTVLKRKLPERLPVSSYDIEEIFFTKNFHPLKGQNSVDTKQK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEECCCCCCCCCCCCCHHHH
RTKFMLKVLGFVFLMRCLLFVASFLSIPIEIVAVLGSLVLLIWRWYYLRTNPVDILKKTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
WHILIFAFSMYVIIYGLHNAGLTTALVNWLEPVVSQHLLYASFAMGGLVSLLSNIFNNHP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ALMIGTITLTEMGLDPITLKTIYLANIIGSDIGSLLLPIGTLASLIWMYILRQNKIKVKW
CEEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
KDYLSVSLIVIPLTTVVTLFLLYYWVHLFFAL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVEIWITIIVFFLTMIVIFWRPRGLNEAWPAAIGAGIILITGLVSKPDVMDIISKIGGA
CEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCHHHHHHHCCCCCHHHHHHHHHCCC
SITILATIVMAVILESFGFFHWSAAKLANLAKGSGRRLYWYIQLLCFLMTLLFNNDGSIL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
ITTPILILLLKNLQLKPHQQIPYLLSGALIATASSAPIGVSNIVNLIALNIVNMTLYMHT
EHHHHHHHHHHHCCCCCCCCCCHHHHCHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
AMMFVPATLGLLFMSWLMYTVLKRKLPERLPVSSYDIEEIFFTKNFHPLKGQNSVDTKQK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEECCCCCCCCCCCCCHHHH
RTKFMLKVLGFVFLMRCLLFVASFLSIPIEIVAVLGSLVLLIWRWYYLRTNPVDILKKTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
WHILIFAFSMYVIIYGLHNAGLTTALVNWLEPVVSQHLLYASFAMGGLVSLLSNIFNNHP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ALMIGTITLTEMGLDPITLKTIYLANIIGSDIGSLLLPIGTLASLIWMYILRQNKIKVKW
CEEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
KDYLSVSLIVIPLTTVVTLFLLYYWVHLFFAL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]