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The map label for this gene is celB2 [H]

Identifier: 218906385

GI number: 218906385

Start: 4993306

End: 4994610

Strand: Reverse

Name: celB2 [H]

Synonym: BCAH820_5299

Alternate gene names: 218906385

Gene position: 4994610-4993306 (Counterclockwise)

Preceding gene: 218906386

Following gene: 218906384

Centisome position: 94.19

GC content: 38.01

Gene sequence:

>1305_bases
ATGCAAAAGTTTATTGCATTTATGGAGAAGTACATGGTTCCGATTGCTGGTAAAATCGGATCGCAACGTCACTTAGCTGC
GATCCGAGATGGATTTATTGCAGTTATGCCACTTATTTTAGTTGGTTCGATGGCAGTACTAGTTAATGGTTTACCAATTC
CAGCATTCCAAGGTTTTATGAAAGATTTATTCGGTGAAACTTGGACACAAATTGGTGCTGGTATGTGGACAGGGTCCTTC
GGGGTATTAGCTCTGATGGTGGCGGTTACCATAAGTTATAACTTAGCAAAATCTTACGGTGTTGATGGATTGTCAGCAGG
TATTATTTCGTTTGGTGCATTAATTATTCTTACACCAACAACACCAAAAGAAGCTGGAATAAATATGCTTTGGACAGGCG
CACAGGGTTTGTTCGTTTCACTTATTGTCTCCATACTCGTAACGGAGATATTCCGCTTCTTTGTTCAAAGAAACATTACA
ATTAAAATGCCTGACGGTGTACCACCAGCAGTAATGAAATCTTTCGCTGCTTTAGTTCCAGCATTTGTGATTTTAACAGG
TATTGCAAGTATTCAATTAGCAGTAAAACTAGCAGGTACAAGTGTTCATGAGTATATCTTTAATACATTACAAGTTCCAT
TACAAGGTTTAGCGGGTACGTTACCAAGTGCAATCTTTATTGCGTTATTAGTACATGTACTTTGGTTCTTCGGTTTACAT
GGTCCAAATATTGTTGGTGGTATTATTGAGCCACTTTATTTACCGGCGTTAGAGAAAAATATTAAGTTATTCCAAGATGG
TACATCTGCATTTGATGTTCCAAACATTATTACAAAACCATTCTTCGATACTTTCGTATATCTTGGTGGTTCTGGCGCAA
CATTAGCGTTCTTAGTGGTTGTATTAATTGTAGCGAAAAGTGCACAATTACGCGGTGTATCTCGTCTATCAATTGGACCT
GGTGCATTTAACATTAACGAACCAGTAATCTTTGGTACACCAATCGTATTAAATCCAATTTTATTCATCCCGTTCGTATT
AACACCTGTTGTGTTAGTTATTACATCTTATACAGCAATTTACTTAGGATGGGTACCAAAAACAGTAGCGATGGTTCCAT
GGAACGTTCCACCAATTATTAGTGGGTATCTTGTAACAGGTTTACATCCTTCTGGTGCGATTTTACAATTATTCAACTTT
GTTGTTGCAATGGCGATTTACTTCCCATTCGTTGTCGCATGCGATCGTTCAGTTATCCGTACTGAGAAAGCAGCACAAGG
AAATAACAACTCTGTACCTATGTAA

Upstream 100 bases:

>100_bases
GGAATCGACGTTATCAATCCAATGCACTACGGCATGATGAATGGAGAAGCAGTTTTAGATCACGCATTAACACTTAAAAA
ATAATTAGGGGGGAAGTAAA

Downstream 100 bases:

>100_bases
CATAATAATTGTTCTCACAGACAACATGATCTGTGAGAACAATTACCTTTATATCATGTTCAAAAAGATGATACTCATCT
ACAAGAACACCAAACTATCA

Product: PTS system, cellobiose-specific IIC component

Products: protein histidine; sugar phosphate

Alternate protein names: EIIC-Cel; PTS system cellobiose-specific EIIC component [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MQKFIAFMEKYMVPIAGKIGSQRHLAAIRDGFIAVMPLILVGSMAVLVNGLPIPAFQGFMKDLFGETWTQIGAGMWTGSF
GVLALMVAVTISYNLAKSYGVDGLSAGIISFGALIILTPTTPKEAGINMLWTGAQGLFVSLIVSILVTEIFRFFVQRNIT
IKMPDGVPPAVMKSFAALVPAFVILTGIASIQLAVKLAGTSVHEYIFNTLQVPLQGLAGTLPSAIFIALLVHVLWFFGLH
GPNIVGGIIEPLYLPALEKNIKLFQDGTSAFDVPNIITKPFFDTFVYLGGSGATLAFLVVVLIVAKSAQLRGVSRLSIGP
GAFNINEPVIFGTPIVLNPILFIPFVLTPVVLVITSYTAIYLGWVPKTVAMVPWNVPPIISGYLVTGLHPSGAILQLFNF
VVAMAIYFPFVVACDRSVIRTEKAAQGNNNSVPM

Sequences:

>Translated_434_residues
MQKFIAFMEKYMVPIAGKIGSQRHLAAIRDGFIAVMPLILVGSMAVLVNGLPIPAFQGFMKDLFGETWTQIGAGMWTGSF
GVLALMVAVTISYNLAKSYGVDGLSAGIISFGALIILTPTTPKEAGINMLWTGAQGLFVSLIVSILVTEIFRFFVQRNIT
IKMPDGVPPAVMKSFAALVPAFVILTGIASIQLAVKLAGTSVHEYIFNTLQVPLQGLAGTLPSAIFIALLVHVLWFFGLH
GPNIVGGIIEPLYLPALEKNIKLFQDGTSAFDVPNIITKPFFDTFVYLGGSGATLAFLVVVLIVAKSAQLRGVSRLSIGP
GAFNINEPVIFGTPIVLNPILFIPFVLTPVVLVITSYTAIYLGWVPKTVAMVPWNVPPIISGYLVTGLHPSGAILQLFNF
VVAMAIYFPFVVACDRSVIRTEKAAQGNNNSVPM
>Mature_434_residues
MQKFIAFMEKYMVPIAGKIGSQRHLAAIRDGFIAVMPLILVGSMAVLVNGLPIPAFQGFMKDLFGETWTQIGAGMWTGSF
GVLALMVAVTISYNLAKSYGVDGLSAGIISFGALIILTPTTPKEAGINMLWTGAQGLFVSLIVSILVTEIFRFFVQRNIT
IKMPDGVPPAVMKSFAALVPAFVILTGIASIQLAVKLAGTSVHEYIFNTLQVPLQGLAGTLPSAIFIALLVHVLWFFGLH
GPNIVGGIIEPLYLPALEKNIKLFQDGTSAFDVPNIITKPFFDTFVYLGGSGATLAFLVVVLIVAKSAQLRGVSRLSIGP
GAFNINEPVIFGTPIVLNPILFIPFVLTPVVLVITSYTAIYLGWVPKTVAMVPWNVPPIISGYLVTGLHPSGAILQLFNF
VVAMAIYFPFVVACDRSVIRTEKAAQGNNNSVPM

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=452, Percent_Identity=34.9557522123894, Blast_Score=265, Evalue=4e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: 2.7.1.69

Molecular weight: Translated: 46488; Mature: 46488

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCCCCCCCC
>Mature Secondary Structure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HHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8407820 [H]