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The map label for this gene is cysL [H]

Identifier: 218906350

GI number: 218906350

Start: 4956690

End: 4957583

Strand: Direct

Name: cysL [H]

Synonym: BCAH820_5263

Alternate gene names: 218906350

Gene position: 4956690-4957583 (Clockwise)

Preceding gene: 218906323

Following gene: 218906351

Centisome position: 93.48

GC content: 40.49

Gene sequence:

>894_bases
ATGAACGTAGATATTTTAAAAATCTTTGTTACTGTCGTTGAGCAGAAACATTTCTCCCGTGCAGCAGAATTATTAAACCT
TTCACAGCCTGGCGTAAGTATGCATATACGCAACTTAGAAAATGAATTTGGAACTACACTCATTCAGCGCTCACCGAAGC
ACGTTCAAGTGACCGAGGCCGGAAACATCTTATATATACATGCAAAGCAAATGCTCTCTCTTTACGAAGATGCTAAGCAA
GAAATTAACGAGCTACATAACGTTGTAACAGGAACTCTTCGCATCGGTGCTAGTTTTACAATTGGCGAATACTTACTTCC
GAAAATACTAGCTAACTACGCGAATGAAAATCCACACGTCGAAGTTCACACCTTCATTTCAAATACAGAAGACGTTTTGC
AAAGCCTTCGCTCTAATCAAATTGATATCGGCTTAGTAGAAGGGCAAGTCGTATACGCTGACGTTGACGTTGAAACATTT
ATGCAAGATGAAATGAAGCTCGTCGTTCCACCAAACCATCCACTGCTCCGCACAAATGAAGTGAAGGAAAACACACTCCA
AGATCAAGTATGGGTATTAAGAGAAAGTGGTTCTGGTACACGCGCTTATAGTGACCGCTTTATTCATCAACACCATTTAA
AAATGAAGCGATTTTTTACATTCAGCAGTATCCAAAGCGTGAAGGAAGCGGTGAGTGCTGGGCTTGGCATCGCTATACTT
TCTGATTGGACTGTACGGAAAGAGCTACTTGCAAAAGAACTATTCCACGTCGAAGTTCCAAATGAACAACTCATCCGGCC
ATTTTCCATCGTCCGTGGCAAATATTTCATCCCATCTAAAGCTATTCAAGTGTTTTTAAATCATGTGAATTCTTTTGCGA
AAAAACAGCATTGA

Upstream 100 bases:

>100_bases
CATTATAAGAGAGTTTCTATAATAAGTTTAATACCAATACCAAATCTCTATCATCAGAAATACTTATGATAAGATGATAG
AAAAGAAAGGAGCCGATTAT

Downstream 100 bases:

>100_bases
CTTTCACATTAGTGTGAAGGTTTACAATACATGTAAAAGGAGTGAAACACATTGCGAATTGGAGAGTTATCAAAAGAAAC
TGGCGTTAGTGAAAGATCGC

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRSPKHVQVTEAGNILYIHAKQMLSLYEDAKQ
EINELHNVVTGTLRIGASFTIGEYLLPKILANYANENPHVEVHTFISNTEDVLQSLRSNQIDIGLVEGQVVYADVDVETF
MQDEMKLVVPPNHPLLRTNEVKENTLQDQVWVLRESGSGTRAYSDRFIHQHHLKMKRFFTFSSIQSVKEAVSAGLGIAIL
SDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNSFAKKQH

Sequences:

>Translated_297_residues
MNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRSPKHVQVTEAGNILYIHAKQMLSLYEDAKQ
EINELHNVVTGTLRIGASFTIGEYLLPKILANYANENPHVEVHTFISNTEDVLQSLRSNQIDIGLVEGQVVYADVDVETF
MQDEMKLVVPPNHPLLRTNEVKENTLQDQVWVLRESGSGTRAYSDRFIHQHHLKMKRFFTFSSIQSVKEAVSAGLGIAIL
SDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNSFAKKQH
>Mature_297_residues
MNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRSPKHVQVTEAGNILYIHAKQMLSLYEDAKQ
EINELHNVVTGTLRIGASFTIGEYLLPKILANYANENPHVEVHTFISNTEDVLQSLRSNQIDIGLVEGQVVYADVDVETF
MQDEMKLVVPPNHPLLRTNEVKENTLQDQVWVLRESGSGTRAYSDRFIHQHHLKMKRFFTFSSIQSVKEAVSAGLGIAIL
SDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNSFAKKQH

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=285, Percent_Identity=32.9824561403509, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1787879, Length=302, Percent_Identity=29.4701986754967, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1790399, Length=304, Percent_Identity=26.9736842105263, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1788748, Length=235, Percent_Identity=27.6595744680851, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI157672245, Length=223, Percent_Identity=26.457399103139, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI87081904, Length=291, Percent_Identity=25.4295532646048, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI145693105, Length=235, Percent_Identity=23.8297872340426, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788297, Length=249, Percent_Identity=26.5060240963855, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786401, Length=297, Percent_Identity=26.2626262626263, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI48994962, Length=146, Percent_Identity=32.1917808219178, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787601, Length=191, Percent_Identity=25.130890052356, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787530, Length=248, Percent_Identity=27.0161290322581, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788887, Length=183, Percent_Identity=30.0546448087432, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI2367136, Length=245, Percent_Identity=24.8979591836735, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87082132, Length=254, Percent_Identity=25.1968503937008, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788296, Length=245, Percent_Identity=24.0816326530612, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789639, Length=118, Percent_Identity=33.0508474576271, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790208, Length=247, Percent_Identity=23.0769230769231, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789204, Length=310, Percent_Identity=23.5483870967742, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789440, Length=118, Percent_Identity=31.3559322033898, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787589, Length=256, Percent_Identity=25, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787128, Length=320, Percent_Identity=22.5, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789173, Length=255, Percent_Identity=23.5294117647059, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789506, Length=196, Percent_Identity=23.469387755102, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI145693193, Length=117, Percent_Identity=31.6239316239316, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790783, Length=254, Percent_Identity=24.4094488188976, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33863; Mature: 33863

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRSPKHVQVTEA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECC
GNILYIHAKQMLSLYEDAKQEINELHNVVTGTLRIGASFTIGEYLLPKILANYANENPHV
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCCCCCE
EVHTFISNTEDVLQSLRSNQIDIGLVEGQVVYADVDVETFMQDEMKLVVPPNHPLLRTNE
EEEEECCCHHHHHHHHHCCCCEEEEECCEEEEEECCHHHHHCCCCEEEECCCCCCEECCC
VKENTLQDQVWVLRESGSGTRAYSDRFIHQHHLKMKRFFTFSSIQSVKEAVSAGLGIAIL
HHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
SDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNSFAKKQH
CCHHHHHHHHHHHHHEEECCCHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRSPKHVQVTEA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECC
GNILYIHAKQMLSLYEDAKQEINELHNVVTGTLRIGASFTIGEYLLPKILANYANENPHV
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCCCCCE
EVHTFISNTEDVLQSLRSNQIDIGLVEGQVVYADVDVETFMQDEMKLVVPPNHPLLRTNE
EEEEECCCHHHHHHHHHCCCCEEEEECCEEEEEECCHHHHHCCCCEEEECCCCCCEECCC
VKENTLQDQVWVLRESGSGTRAYSDRFIHQHHLKMKRFFTFSSIQSVKEAVSAGLGIAIL
HHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
SDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNSFAKKQH
CCHHHHHHHHHHHHHEEECCCHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]