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The map label for this gene is ag45 [H]

Identifier: 218906110

GI number: 218906110

Start: 4731073

End: 4732398

Strand: Direct

Name: ag45 [H]

Synonym: BCAH820_5022

Alternate gene names: 218906110

Gene position: 4731073-4732398 (Clockwise)

Preceding gene: 218906108

Following gene: 218906112

Centisome position: 89.22

GC content: 37.41

Gene sequence:

>1326_bases
TTGCACGAAACAACGCAAGAGCTCGCAAATTGGCAGTATTATTTTGCTATCGCAGTCTTTTTAATTACATATGCCATTAT
TATTTCTGAAAAAATCAATCGCGCTGTCATCGCACTTCTCGGTGCAGCACTCATGGTAATTATGGGGGTCGTTGATTTAC
ACAACGCCTTTACGAAACATATTGAATGGGGGACGATTACACTACTCATCGGTATGATGATTTTGGTGAACATTACGAGT
AAATCGGGCGTCTTCCAATACGTTGCCATTAAAGCAGCAAAAGGAGCGCAAGGAAATCCAATTAAAATTTTAATTTCACT
TTCCTTACTTACTGCGCTTGGTTCCGCATTTTTAGATAACGTTACAACTGTACTTCTTGTTGTTCCAGTTACTTTATCTA
TTACGCGCATACTGCAAGTAAATCCTGTTCCTTATTTACTTTCTGAAATTATTTTTTCAAACATTGGGGGAACAGCAACA
TTAATTGGTGATCCACCTAATATTATGATTGGTTCTGCAAATAAGCATTTAGACTTCAATGCATTCTTGATAAACTTAGC
GCCAATCGTAATTATTATTATTGCAGTGACAGCAGTCATACTTTACTTCATGTACCGTAAACAATTAATTGCTGATCCTG
TACAAGTTAAGAAATTAATGAGCCTAGATGAAAAACAATACATTAAAGATCCAGTATTAATGAAAAAATCTTTAACAGTA
CTTGGACTTACAATTGTAGGTTTCATGACTCATTCGATTTTCCACATTGATGCAGCTATCATCGCTTTAACTGGTGCTAC
TGTACTTATGCTAATTGCTGTGAAAGAACATGAAATTGAAGAGGTATTTGCAAGTGTAGAATGGGTAACAATCTTCTTCT
TCGCAGGACTATTCGTACTCGTTGGAGGACTTATCGATATCGGCCTTATTAAAATGCTAGCTCAAAAAGTAATTGGCATA
ACAGGCGGAGATATTTCTTACGCATCTATCCTTATTTTATGGGTGTCTGGTATCGCCTCTGCAACAATTGATAACATCCC
ATTCGTTGCAACAATGATCCCACTTATTAACGATATGGCAGTTGGGCTAGGTTTATCACCTTCTGACGCACAAATCGACG
TATTATGGTGGGCATTAGCATTAGGTGCTTGTCTGGGCGGAAACGGAACATTAATCGGAGCTTCAGCTAACGTAATTGTC
GCAGGTATCGCAAGCCGCGAAGGACATAAATTTAGCTACATGGACTTCCTAAAAGTCGGTTTCCCAATTATGATCGTTTC
ATTAATCATTTCTCATATTTATATTTATTTACGCTACCTTATGTAG

Upstream 100 bases:

>100_bases
TTTACATCCGACAATTTTCTAATTTATAATGACGTTATGTGTGTTTCATCTAGTTAATTTTTGACACACTAAGAAAAGTG
AATGAAAGGAGATGAGAGTT

Downstream 100 bases:

>100_bases
TAGAAAAAGCGCTCTGATAATCAGGGCGCTTTTTTCATTACACATATTTAAAATAGACCTACTCGTTATACCTCACCGGC
TCATTTCGAATAATGAACAA

Product: arsenical pump family protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MHETTQELANWQYYFAIAVFLITYAIIISEKINRAVIALLGAALMVIMGVVDLHNAFTKHIEWGTITLLIGMMILVNITS
KSGVFQYVAIKAAKGAQGNPIKILISLSLLTALGSAFLDNVTTVLLVVPVTLSITRILQVNPVPYLLSEIIFSNIGGTAT
LIGDPPNIMIGSANKHLDFNAFLINLAPIVIIIIAVTAVILYFMYRKQLIADPVQVKKLMSLDEKQYIKDPVLMKKSLTV
LGLTIVGFMTHSIFHIDAAIIALTGATVLMLIAVKEHEIEEVFASVEWVTIFFFAGLFVLVGGLIDIGLIKMLAQKVIGI
TGGDISYASILILWVSGIASATIDNIPFVATMIPLINDMAVGLGLSPSDAQIDVLWWALALGACLGGNGTLIGASANVIV
AGIASREGHKFSYMDFLKVGFPIMIVSLIISHIYIYLRYLM

Sequences:

>Translated_441_residues
MHETTQELANWQYYFAIAVFLITYAIIISEKINRAVIALLGAALMVIMGVVDLHNAFTKHIEWGTITLLIGMMILVNITS
KSGVFQYVAIKAAKGAQGNPIKILISLSLLTALGSAFLDNVTTVLLVVPVTLSITRILQVNPVPYLLSEIIFSNIGGTAT
LIGDPPNIMIGSANKHLDFNAFLINLAPIVIIIIAVTAVILYFMYRKQLIADPVQVKKLMSLDEKQYIKDPVLMKKSLTV
LGLTIVGFMTHSIFHIDAAIIALTGATVLMLIAVKEHEIEEVFASVEWVTIFFFAGLFVLVGGLIDIGLIKMLAQKVIGI
TGGDISYASILILWVSGIASATIDNIPFVATMIPLINDMAVGLGLSPSDAQIDVLWWALALGACLGGNGTLIGASANVIV
AGIASREGHKFSYMDFLKVGFPIMIVSLIISHIYIYLRYLM
>Mature_441_residues
MHETTQELANWQYYFAIAVFLITYAIIISEKINRAVIALLGAALMVIMGVVDLHNAFTKHIEWGTITLLIGMMILVNITS
KSGVFQYVAIKAAKGAQGNPIKILISLSLLTALGSAFLDNVTTVLLVVPVTLSITRILQVNPVPYLLSEIIFSNIGGTAT
LIGDPPNIMIGSANKHLDFNAFLINLAPIVIIIIAVTAVILYFMYRKQLIADPVQVKKLMSLDEKQYIKDPVLMKKSLTV
LGLTIVGFMTHSIFHIDAAIIALTGATVLMLIAVKEHEIEEVFASVEWVTIFFFAGLFVLVGGLIDIGLIKMLAQKVIGI
TGGDISYASILILWVSGIASATIDNIPFVATMIPLINDMAVGLGLSPSDAQIDVLWWALALGACLGGNGTLIGASANVIV
AGIASREGHKFSYMDFLKVGFPIMIVSLIISHIYIYLRYLM

Specific function: Involved In Arsenical Resistance. Thought To Form The Channel Of An Arsenite Pump (By Similarity). [C]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CitM (TC 2.A.11) transporter family [H]

Homologues:

Organism=Homo sapiens, GI157266326, Length=507, Percent_Identity=35.7001972386588, Blast_Score=245, Evalue=5e-65,
Organism=Escherichia coli, GI87082277, Length=424, Percent_Identity=23.8207547169811, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574683, Length=501, Percent_Identity=35.7285429141717, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI28574681, Length=501, Percent_Identity=35.7285429141717, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI28574685, Length=501, Percent_Identity=35.7285429141717, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI28574678, Length=501, Percent_Identity=35.7285429141717, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI19920704, Length=501, Percent_Identity=35.7285429141717, Blast_Score=252, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24581759, Length=501, Percent_Identity=35.7285429141717, Blast_Score=252, Evalue=3e-67,
Organism=Drosophila melanogaster, GI221472711, Length=495, Percent_Identity=29.0909090909091, Blast_Score=188, Evalue=8e-48,
Organism=Drosophila melanogaster, GI24581764, Length=495, Percent_Identity=29.0909090909091, Blast_Score=185, Evalue=6e-47,
Organism=Drosophila melanogaster, GI45550608, Length=475, Percent_Identity=31.5789473684211, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI85724998, Length=474, Percent_Identity=25.1054852320675, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI62484377, Length=470, Percent_Identity=25.3191489361702, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24656008, Length=473, Percent_Identity=27.906976744186, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802
- InterPro:   IPR004680 [H]

Pfam domain/function: PF03600 CitMHS [H]

EC number: NA

Molecular weight: Translated: 47719; Mature: 47719

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHETTQELANWQYYFAIAVFLITYAIIISEKINRAVIALLGAALMVIMGVVDLHNAFTKH
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEWGTITLLIGMMILVNITSKSGVFQYVAIKAAKGAQGNPIKILISLSLLTALGSAFLDN
CCCHHHHHHHHHHHHHEECCCCCCEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHH
VTTVLLVVPVTLSITRILQVNPVPYLLSEIIFSNIGGTATLIGDPPNIMIGSANKHLDFN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCCCHH
AFLINLAPIVIIIIAVTAVILYFMYRKQLIADPVQVKKLMSLDEKQYIKDPVLMKKSLTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHCCHHHHHHHHHH
LGLTIVGFMTHSIFHIDAAIIALTGATVLMLIAVKEHEIEEVFASVEWVTIFFFAGLFVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGGLIDIGLIKMLAQKVIGITGGDISYASILILWVSGIASATIDNIPFVATMIPLINDMA
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VGLGLSPSDAQIDVLWWALALGACLGGNGTLIGASANVIVAGIASREGHKFSYMDFLKVG
HCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCCHHHHHHHHH
FPIMIVSLIISHIYIYLRYLM
HHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MHETTQELANWQYYFAIAVFLITYAIIISEKINRAVIALLGAALMVIMGVVDLHNAFTKH
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEWGTITLLIGMMILVNITSKSGVFQYVAIKAAKGAQGNPIKILISLSLLTALGSAFLDN
CCCHHHHHHHHHHHHHEECCCCCCEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHH
VTTVLLVVPVTLSITRILQVNPVPYLLSEIIFSNIGGTATLIGDPPNIMIGSANKHLDFN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCCCHH
AFLINLAPIVIIIIAVTAVILYFMYRKQLIADPVQVKKLMSLDEKQYIKDPVLMKKSLTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHCCHHHHHHHHHH
LGLTIVGFMTHSIFHIDAAIIALTGATVLMLIAVKEHEIEEVFASVEWVTIFFFAGLFVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGGLIDIGLIKMLAQKVIGITGGDISYASILILWVSGIASATIDNIPFVATMIPLINDMA
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VGLGLSPSDAQIDVLWWALALGACLGGNGTLIGASANVIVAGIASREGHKFSYMDFLKVG
HCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCCHHHHHHHHH
FPIMIVSLIISHIYIYLRYLM
HHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]