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The map label for this gene is bmr3 [H]

Identifier: 218905939

GI number: 218905939

Start: 4569600

End: 4571009

Strand: Direct

Name: bmr3 [H]

Synonym: BCAH820_4827

Alternate gene names: 218905939

Gene position: 4569600-4571009 (Clockwise)

Preceding gene: 218905938

Following gene: 218905942

Centisome position: 86.18

GC content: 39.5

Gene sequence:

>1410_bases
ATGCTAATGACGTTTCTTTCTGCAGTAGAAGGAACGATTGTTAGTACAGCAATCCCTCGTATAACGAGTGATTTGTCAGG
CGTTGAACTTGTTAGCTGGGTTTACGCAATTTACATGCTTGCCACAGCGGTTTCAACTCCAATATACGGAAAGCTAGCTG
ATTTATTTGGCCGTAAAAAAGTTTTACTTATTGGAGCTACAATCTTTTTAGTCGGTTCTGCACTTTGCGGTGTCGTTACA
TCAATGGAACAATTAATCTTCTTCCGCGCACTTCAAGGTATCGGTGCTGGTGCTGTTATGCCGATCACAATGACGATTAT
TGGAGACTTATATAGCGAAGCGAAAGACCGCGCGAAAGCACAAGGCTGGATGAGTGCTGTTTGGGGCGTCTCAGGTGTTA
TCGGACCATTAGTCGGCGGATTTTTAGTTGATTCTCTTTCTTGGCGTTATATTTTCTTCTTAAACGTTCCATTTGGAATT
ATCGCTTGCTTAATGATTGCAATTTATTACAAAGAATCTATTAAGCCTGCTAAGCACCATATCGACTATCTTGGTGCAAC
AGTATTCTCACTAAGTACGATTGCTCTACTATACGCATTATTAACAGGTAGCAGTAAGCAAAACTGGGGAGATGTAACAA
TTATCGGCCTATTAATCTTCGCCGTCGTTTCATTCATTATTTTCTTATTCATCGAGAAAAAATCTCCGGAACCATTAATT
CCGCTAGCACTTTTCTCTAACCGTACATTATCTACGATAAATATATTAACGCTTATCGCTGGCGCAATGATTATTAGTAT
TACAATGTACCTTCCAATTTGGAGCCAAGGTGTATTAGGGAAAAATGCGACAGAAGCCGGACTTATTTTAATGCCAATCC
CTGTTATGTGGACATTTGGCGCTATTTTCTCTGGTAACTTAGTCGGCAAATTAAAAACGAAACAAATCATTTTACTTGGA
GCTAGCATTTTATCAGTCGCTACGTTCTTATTATTTACATTATCAACAAATTCGCCATCGTTCCTTATTTATGTAGCAGT
CGGATTATTTGGCTTAGGAATGGGACTTGTGACACCGATTTATATGGTAACAATTCAAGCAGCCGTACCAGCTCATACAC
GCGGAACAGCCGTTGGTTTAAACACATTTATCAATACATTTAGCCAAACATTAGGTGCGGCAGTATTCGGTACAATCTTT
AATACGATGATTCACGCACGTGGCATTAAAAACCTTGATCTTGTATCTGGCGGACACGGCGGAGCTACAGCAAACGTAGT
AACTGAATCACAATCTGCATTAGCATCAAGTGTTCACGTTATTTATATGAGCACTTTCGTATTAGCAGTATGTACATTAA
TTATCGCTTGGCTTTTATTAAAGCCCGCTACACAAACAAGTGAGCAATAA

Upstream 100 bases:

>100_bases
TGTTGCTTTATATACCTTAGTACCTTTATATTTTATATTGTTGAATATTTGAAAAAGGGTAAGGGGAAAACATATGAGAA
AAAAAGTCATGATGTCTTTA

Downstream 100 bases:

>100_bases
GAAAGAGGATGACCATTTCGGTCATCCTCTTTTACTCTTCATTCTCCACAATATGTTTCAGCATGGACAATTTATTATCC
CAAAAACGTTCATAATATGA

Product: drug resistance transporter, EmrB/QacA family

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MLMTFLSAVEGTIVSTAIPRITSDLSGVELVSWVYAIYMLATAVSTPIYGKLADLFGRKKVLLIGATIFLVGSALCGVVT
SMEQLIFFRALQGIGAGAVMPITMTIIGDLYSEAKDRAKAQGWMSAVWGVSGVIGPLVGGFLVDSLSWRYIFFLNVPFGI
IACLMIAIYYKESIKPAKHHIDYLGATVFSLSTIALLYALLTGSSKQNWGDVTIIGLLIFAVVSFIIFLFIEKKSPEPLI
PLALFSNRTLSTINILTLIAGAMIISITMYLPIWSQGVLGKNATEAGLILMPIPVMWTFGAIFSGNLVGKLKTKQIILLG
ASILSVATFLLFTLSTNSPSFLIYVAVGLFGLGMGLVTPIYMVTIQAAVPAHTRGTAVGLNTFINTFSQTLGAAVFGTIF
NTMIHARGIKNLDLVSGGHGGATANVVTESQSALASSVHVIYMSTFVLAVCTLIIAWLLLKPATQTSEQ

Sequences:

>Translated_469_residues
MLMTFLSAVEGTIVSTAIPRITSDLSGVELVSWVYAIYMLATAVSTPIYGKLADLFGRKKVLLIGATIFLVGSALCGVVT
SMEQLIFFRALQGIGAGAVMPITMTIIGDLYSEAKDRAKAQGWMSAVWGVSGVIGPLVGGFLVDSLSWRYIFFLNVPFGI
IACLMIAIYYKESIKPAKHHIDYLGATVFSLSTIALLYALLTGSSKQNWGDVTIIGLLIFAVVSFIIFLFIEKKSPEPLI
PLALFSNRTLSTINILTLIAGAMIISITMYLPIWSQGVLGKNATEAGLILMPIPVMWTFGAIFSGNLVGKLKTKQIILLG
ASILSVATFLLFTLSTNSPSFLIYVAVGLFGLGMGLVTPIYMVTIQAAVPAHTRGTAVGLNTFINTFSQTLGAAVFGTIF
NTMIHARGIKNLDLVSGGHGGATANVVTESQSALASSVHVIYMSTFVLAVCTLIIAWLLLKPATQTSEQ
>Mature_469_residues
MLMTFLSAVEGTIVSTAIPRITSDLSGVELVSWVYAIYMLATAVSTPIYGKLADLFGRKKVLLIGATIFLVGSALCGVVT
SMEQLIFFRALQGIGAGAVMPITMTIIGDLYSEAKDRAKAQGWMSAVWGVSGVIGPLVGGFLVDSLSWRYIFFLNVPFGI
IACLMIAIYYKESIKPAKHHIDYLGATVFSLSTIALLYALLTGSSKQNWGDVTIIGLLIFAVVSFIIFLFIEKKSPEPLI
PLALFSNRTLSTINILTLIAGAMIISITMYLPIWSQGVLGKNATEAGLILMPIPVMWTFGAIFSGNLVGKLKTKQIILLG
ASILSVATFLLFTLSTNSPSFLIYVAVGLFGLGMGLVTPIYMVTIQAAVPAHTRGTAVGLNTFINTFSQTLGAAVFGTIF
NTMIHARGIKNLDLVSGGHGGATANVVTESQSALASSVHVIYMSTFVLAVCTLIIAWLLLKPATQTSEQ

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=426, Percent_Identity=26.5258215962441, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI87081983, Length=400, Percent_Identity=28.25, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788710, Length=494, Percent_Identity=25.3036437246964, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1790195, Length=362, Percent_Identity=27.9005524861878, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788392, Length=294, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1790146, Length=161, Percent_Identity=27.9503105590062, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788509, Length=264, Percent_Identity=25.3787878787879, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI48994889, Length=202, Percent_Identity=29.7029702970297, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=462, Percent_Identity=24.6753246753247, Blast_Score=122, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6323735, Length=303, Percent_Identity=27.7227722772277, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319770, Length=368, Percent_Identity=26.3586956521739, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319783, Length=382, Percent_Identity=24.0837696335079, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321663, Length=463, Percent_Identity=22.6781857451404, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324954, Length=410, Percent_Identity=22.6829268292683, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=141, Percent_Identity=29.7872340425532, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323520, Length=292, Percent_Identity=26.7123287671233, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49991; Mature: 49991

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMTFLSAVEGTIVSTAIPRITSDLSGVELVSWVYAIYMLATAVSTPIYGKLADLFGRKK
CHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCE
VLLIGATIFLVGSALCGVVTSMEQLIFFRALQGIGAGAVMPITMTIIGDLYSEAKDRAKA
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QGWMSAVWGVSGVIGPLVGGFLVDSLSWRYIFFLNVPFGIIACLMIAIYYKESIKPAKHH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHH
IDYLGATVFSLSTIALLYALLTGSSKQNWGDVTIIGLLIFAVVSFIIFLFIEKKSPEPLI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
PLALFSNRTLSTINILTLIAGAMIISITMYLPIWSQGVLGKNATEAGLILMPIPVMWTFG
EHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHH
AIFSGNLVGKLKTKQIILLGASILSVATFLLFTLSTNSPSFLIYVAVGLFGLGMGLVTPI
HHHCCCCEEEECCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHH
YMVTIQAAVPAHTRGTAVGLNTFINTFSQTLGAAVFGTIFNTMIHARGIKNLDLVSGGHG
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCC
GATANVVTESQSALASSVHVIYMSTFVLAVCTLIIAWLLLKPATQTSEQ
CCEEHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLMTFLSAVEGTIVSTAIPRITSDLSGVELVSWVYAIYMLATAVSTPIYGKLADLFGRKK
CHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCE
VLLIGATIFLVGSALCGVVTSMEQLIFFRALQGIGAGAVMPITMTIIGDLYSEAKDRAKA
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QGWMSAVWGVSGVIGPLVGGFLVDSLSWRYIFFLNVPFGIIACLMIAIYYKESIKPAKHH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHH
IDYLGATVFSLSTIALLYALLTGSSKQNWGDVTIIGLLIFAVVSFIIFLFIEKKSPEPLI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
PLALFSNRTLSTINILTLIAGAMIISITMYLPIWSQGVLGKNATEAGLILMPIPVMWTFG
EHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHH
AIFSGNLVGKLKTKQIILLGASILSVATFLLFTLSTNSPSFLIYVAVGLFGLGMGLVTPI
HHHCCCCEEEECCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHH
YMVTIQAAVPAHTRGTAVGLNTFINTFSQTLGAAVFGTIFNTMIHARGIKNLDLVSGGHG
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCC
GATANVVTESQSALASSVHVIYMSTFVLAVCTLIIAWLLLKPATQTSEQ
CCEEHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]