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The map label for this gene is amyX [H]

Identifier: 218905931

GI number: 218905931

Start: 4560346

End: 4562487

Strand: Reverse

Name: amyX [H]

Synonym: BCAH820_4819

Alternate gene names: 218905931

Gene position: 4562487-4560346 (Counterclockwise)

Preceding gene: 218905932

Following gene: 218905930

Centisome position: 86.04

GC content: 37.35

Gene sequence:

>2142_bases
ATGTTGAAAGTAAAACGTCCATTTGATGCCTATTTAGATGAAATGAATAAAATTACAATTTTGCTCCCACATGCGTATGG
AACAAGTCGGACATTTCGTTTGCAAGAAGGAAGTAACGTGAAAGATTTACCGATTGCTCATACAATTGCGCTTCCGGATG
CAACGAAGTATGAGTGCTTTATAGAAGAGCCGCTAGATGTGGGGAAGTATTATACAGTACGAGATGAACGGAATGAAGAA
ACAGATTTACAAATAGGTGCTGTCATAAGAACAGAGATTTTCGATGAAAAGTATTATTACGAAGGTACAGATTTAGGGGC
TGTATATCAAAAAGAAGCAACTACATTTAAAGTATGGGCTCCAACTGCGAGGCTTGCGAAAGTAAGAATTTATAAAAGTG
ATAAAGAATATACCGATCACGAAATGCACAGAGAAGAAAATGGGGTATGGGCTCATACATTACAAGGCGATTTAGCCGGG
GAAAAGTATACATTTCTTGTTTGTATTAATTTAATATGGAATGAAGCTGTGGATCCTTATGCAAAATCAGTGACTGTAAA
TGGGAAGTACGGCGTTGTTATCGATTTGGAAAAAACAAATGTAACAAAGCAAGAGCAATTACCACCATTACAAGCAATGA
CAGATGCTATATTGTATGAACTGCATATTCGTGATGCTACTATTCATGAGGGCAGTGGAGTGAGTAAAAAAGGAACGTAT
AAAGGGCTAATGGAAGAAGGAACAACAGGGCGAAATGGGACGTTAACAGGTTTGTCTCATATAAAAGATTTAGGTGTTAC
ACATGTTGAATTATTGCCTTTATATTGTTTCGGTGGTGTAGATGAGGCGAATCCTTCTTCTGCATATAATTGGGGTTACA
ATCCATTATATTACAACGCGCCAACGGGGTTTTATGCTACAAACCCATCTGACCCGTATAACAGGATCTTAGAGTGTAAG
CAACTGATTGAAACATTTCATGAACACGGCATTAGGGTGATTATAGATGTTGTATATAATCATGTGTATGAAAGAGAGCT
ATCATCATTTGAAAAGCTTGTCCCAGGATATTATTTTCGTCATGGTGAAAATGGTATGCCTTCTAATGGGACGGGAGTCG
GAAATGATATAGCATCTGAACGGAAAATGATGAGGAAATTCATTGTAGAGTCTATTTTATATTGGCTTACGGAATACAAT
GTTGATGGATTCCGGTTCGACTTAATGGGAATTTTAGATGTTGATACAATAAATATAATAGAAAAAGAAGTGCGAAACAT
AAAGCGAGATGCATTGCTATTAGGAGAAGGGTGGGATCTGCAAACACCACTTCCTCTAGAGGAAAAAGCAACGTTAAATA
ATGCAAACAAAATGCCGCATATTGCGCAATTTAATGATCAATTTCGTGATGGGATAAAGGGAAGTACCTTTCATATAAAT
AAACGTGGTTTTGCATTTGGTGGGCATGTAGACCGTAATCATTTGCAGTATATAGTATCAGGAAGCCTTTTGAGTATGAA
AGAAACAGGTTTGTTTCTAGAGCCTGTGCAAAGCATTAACTATGTAGAATGTCATGACAATATGACGATGTGGGATAAAC
TAATGCGCAGTAATGAAGAGTCGAAAGAGATTTTGAAAAAACGTCACGTGCTAGCAACTGCAATGGTGATTCTGTCGCAA
GGCATCCCTTTCTTACATGCGGGGCAAGAGTTTTATCGTACGAAGCAAGGGAATGAAAATAGTTATAATGCAAATGATGA
AATTAATCAATTAGATTGGGATCGAAAAGAGAAAGAAATAGAGACCGTTAACTATATAAAAGGTTTAATTGCGATTCGTA
AAGAGCACGGAGCATTCCGATTACAAAACGCAGACTTAATAAAAAAGCACATGACTTTTTTGCAAACATCTCCAGAAGTA
CTTGCATATCACTTAGAGCACGTAGAATCGTTTGGACCGTGGAAAGAGATTGCCGTTTTATTTAATAGCAGTTTAGAAGC
GAAAACGGTGCAACTTCCGAAAGAAGAAACGTGGCATGTGTTAGTAAACGAAAAGCAAGCGAAAATAGAGCCAATTTCTT
CATTTAGAGGAAAGGAACTTAGGCTCTCTCCAATTAGCACGTATATATTGACGATAATGTGA

Upstream 100 bases:

>100_bases
ATAAAGAATAATGTGTTACTGTATAGGGCTTCGCTGATAAGAAAAGCCTTATCATTTGCAGCTCGTTTCATTTTAACTTT
AAGAATGATGGTGATTTTTT

Downstream 100 bases:

>100_bases
CAAATCGCTACTTGACGGTGAGAATGTAATAAATGTAGAATTGACAAAGGTGACGATTTTGATAAGTTGTCATTCTATGG
CTGGTTTTGTAAAATAAAAT

Product: putative pullulanase

Products: NA

Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]

Number of amino acids: Translated: 713; Mature: 713

Protein sequence:

>713_residues
MLKVKRPFDAYLDEMNKITILLPHAYGTSRTFRLQEGSNVKDLPIAHTIALPDATKYECFIEEPLDVGKYYTVRDERNEE
TDLQIGAVIRTEIFDEKYYYEGTDLGAVYQKEATTFKVWAPTARLAKVRIYKSDKEYTDHEMHREENGVWAHTLQGDLAG
EKYTFLVCINLIWNEAVDPYAKSVTVNGKYGVVIDLEKTNVTKQEQLPPLQAMTDAILYELHIRDATIHEGSGVSKKGTY
KGLMEEGTTGRNGTLTGLSHIKDLGVTHVELLPLYCFGGVDEANPSSAYNWGYNPLYYNAPTGFYATNPSDPYNRILECK
QLIETFHEHGIRVIIDVVYNHVYERELSSFEKLVPGYYFRHGENGMPSNGTGVGNDIASERKMMRKFIVESILYWLTEYN
VDGFRFDLMGILDVDTINIIEKEVRNIKRDALLLGEGWDLQTPLPLEEKATLNNANKMPHIAQFNDQFRDGIKGSTFHIN
KRGFAFGGHVDRNHLQYIVSGSLLSMKETGLFLEPVQSINYVECHDNMTMWDKLMRSNEESKEILKKRHVLATAMVILSQ
GIPFLHAGQEFYRTKQGNENSYNANDEINQLDWDRKEKEIETVNYIKGLIAIRKEHGAFRLQNADLIKKHMTFLQTSPEV
LAYHLEHVESFGPWKEIAVLFNSSLEAKTVQLPKEETWHVLVNEKQAKIEPISSFRGKELRLSPISTYILTIM

Sequences:

>Translated_713_residues
MLKVKRPFDAYLDEMNKITILLPHAYGTSRTFRLQEGSNVKDLPIAHTIALPDATKYECFIEEPLDVGKYYTVRDERNEE
TDLQIGAVIRTEIFDEKYYYEGTDLGAVYQKEATTFKVWAPTARLAKVRIYKSDKEYTDHEMHREENGVWAHTLQGDLAG
EKYTFLVCINLIWNEAVDPYAKSVTVNGKYGVVIDLEKTNVTKQEQLPPLQAMTDAILYELHIRDATIHEGSGVSKKGTY
KGLMEEGTTGRNGTLTGLSHIKDLGVTHVELLPLYCFGGVDEANPSSAYNWGYNPLYYNAPTGFYATNPSDPYNRILECK
QLIETFHEHGIRVIIDVVYNHVYERELSSFEKLVPGYYFRHGENGMPSNGTGVGNDIASERKMMRKFIVESILYWLTEYN
VDGFRFDLMGILDVDTINIIEKEVRNIKRDALLLGEGWDLQTPLPLEEKATLNNANKMPHIAQFNDQFRDGIKGSTFHIN
KRGFAFGGHVDRNHLQYIVSGSLLSMKETGLFLEPVQSINYVECHDNMTMWDKLMRSNEESKEILKKRHVLATAMVILSQ
GIPFLHAGQEFYRTKQGNENSYNANDEINQLDWDRKEKEIETVNYIKGLIAIRKEHGAFRLQNADLIKKHMTFLQTSPEV
LAYHLEHVESFGPWKEIAVLFNSSLEAKTVQLPKEETWHVLVNEKQAKIEPISSFRGKELRLSPISTYILTIM
>Mature_713_residues
MLKVKRPFDAYLDEMNKITILLPHAYGTSRTFRLQEGSNVKDLPIAHTIALPDATKYECFIEEPLDVGKYYTVRDERNEE
TDLQIGAVIRTEIFDEKYYYEGTDLGAVYQKEATTFKVWAPTARLAKVRIYKSDKEYTDHEMHREENGVWAHTLQGDLAG
EKYTFLVCINLIWNEAVDPYAKSVTVNGKYGVVIDLEKTNVTKQEQLPPLQAMTDAILYELHIRDATIHEGSGVSKKGTY
KGLMEEGTTGRNGTLTGLSHIKDLGVTHVELLPLYCFGGVDEANPSSAYNWGYNPLYYNAPTGFYATNPSDPYNRILECK
QLIETFHEHGIRVIIDVVYNHVYERELSSFEKLVPGYYFRHGENGMPSNGTGVGNDIASERKMMRKFIVESILYWLTEYN
VDGFRFDLMGILDVDTINIIEKEVRNIKRDALLLGEGWDLQTPLPLEEKATLNNANKMPHIAQFNDQFRDGIKGSTFHIN
KRGFAFGGHVDRNHLQYIVSGSLLSMKETGLFLEPVQSINYVECHDNMTMWDKLMRSNEESKEILKKRHVLATAMVILSQ
GIPFLHAGQEFYRTKQGNENSYNANDEINQLDWDRKEKEIETVNYIKGLIAIRKEHGAFRLQNADLIKKHMTFLQTSPEV
LAYHLEHVESFGPWKEIAVLFNSSLEAKTVQLPKEETWHVLVNEKQAKIEPISSFRGKELRLSPISTYILTIM

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=589, Percent_Identity=24.1086587436333, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR011840 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.41 [H]

Molecular weight: Translated: 81704; Mature: 81704

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVKRPFDAYLDEMNKITILLPHAYGTSRTFRLQEGSNVKDLPIAHTIALPDATKYECF
CCCCCCCHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCEEEE
IEEPLDVGKYYTVRDERNEETDLQIGAVIRTEIFDEKYYYEGTDLGAVYQKEATTFKVWA
ECCCCCCCCEEEECCCCCCCCCEEEHHEEEHHHHCCCEEECCCCCCCEEECCCCEEEEEC
PTARLAKVRIYKSDKEYTDHEMHREENGVWAHTLQGDLAGEKYTFLVCINLIWNEAVDPY
CCCEEEEEEEEECCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEEHHHHHCCCCH
AKSVTVNGKYGVVIDLEKTNVTKQEQLPPLQAMTDAILYELHIRDATIHEGSGVSKKGTY
HHEEEECCCEEEEEEEECCCCCHHHCCCCHHHHHHHHEEEEEECCEEEECCCCCCCCCCH
KGLMEEGTTGRNGTLTGLSHIKDLGVTHVELLPLYCFGGVDEANPSSAYNWGYNPLYYNA
HHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEHHEECCCCCCCCCCCCCCCCCCEEECC
PTGFYATNPSDPYNRILECKQLIETFHEHGIRVIIDVVYNHVYERELSSFEKLVPGYYFR
CCCCEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEEE
HGENGMPSNGTGVGNDIASERKMMRKFIVESILYWLTEYNVDGFRFDLMGILDVDTINII
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHH
EKEVRNIKRDALLLGEGWDLQTPLPLEEKATLNNANKMPHIAQFNDQFRDGIKGSTFHIN
HHHHHHHHHCEEEEECCCCCCCCCCCCHHHCCCCCCCCCCHHHHCHHHHCCCCCCEEEEE
KRGFAFGGHVDRNHLQYIVSGSLLSMKETGLFLEPVQSINYVECHDNMTMWDKLMRSNEE
CCCEEECCCCCCCHHEEEECCCEEEHHHCCCCHHHHCCCCEEEECCCHHHHHHHHHCCCH
SKEILKKRHVLATAMVILSQGIPFLHAGQEFYRTKQGNENSYNANDEINQLDWDRKEKEI
HHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
ETVNYIKGLIAIRKEHGAFRLQNADLIKKHMTFLQTSPEVLAYHLEHVESFGPWKEIAVL
HHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHH
FNSSLEAKTVQLPKEETWHVLVNEKQAKIEPISSFRGKELRLSPISTYILTIM
HCCCCCCEEEECCCCCCEEEEECCCCCCCCCHHHCCCCEEEECHHCEEEEEEC
>Mature Secondary Structure
MLKVKRPFDAYLDEMNKITILLPHAYGTSRTFRLQEGSNVKDLPIAHTIALPDATKYECF
CCCCCCCHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCEEEE
IEEPLDVGKYYTVRDERNEETDLQIGAVIRTEIFDEKYYYEGTDLGAVYQKEATTFKVWA
ECCCCCCCCEEEECCCCCCCCCEEEHHEEEHHHHCCCEEECCCCCCCEEECCCCEEEEEC
PTARLAKVRIYKSDKEYTDHEMHREENGVWAHTLQGDLAGEKYTFLVCINLIWNEAVDPY
CCCEEEEEEEEECCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEEHHHHHCCCCH
AKSVTVNGKYGVVIDLEKTNVTKQEQLPPLQAMTDAILYELHIRDATIHEGSGVSKKGTY
HHEEEECCCEEEEEEEECCCCCHHHCCCCHHHHHHHHEEEEEECCEEEECCCCCCCCCCH
KGLMEEGTTGRNGTLTGLSHIKDLGVTHVELLPLYCFGGVDEANPSSAYNWGYNPLYYNA
HHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEHHEECCCCCCCCCCCCCCCCCCEEECC
PTGFYATNPSDPYNRILECKQLIETFHEHGIRVIIDVVYNHVYERELSSFEKLVPGYYFR
CCCCEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEEE
HGENGMPSNGTGVGNDIASERKMMRKFIVESILYWLTEYNVDGFRFDLMGILDVDTINII
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHH
EKEVRNIKRDALLLGEGWDLQTPLPLEEKATLNNANKMPHIAQFNDQFRDGIKGSTFHIN
HHHHHHHHHCEEEEECCCCCCCCCCCCHHHCCCCCCCCCCHHHHCHHHHCCCCCCEEEEE
KRGFAFGGHVDRNHLQYIVSGSLLSMKETGLFLEPVQSINYVECHDNMTMWDKLMRSNEE
CCCEEECCCCCCCHHEEEECCCEEEHHHCCCCHHHHCCCCEEEECCCHHHHHHHHHCCCH
SKEILKKRHVLATAMVILSQGIPFLHAGQEFYRTKQGNENSYNANDEINQLDWDRKEKEI
HHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
ETVNYIKGLIAIRKEHGAFRLQNADLIKKHMTFLQTSPEVLAYHLEHVESFGPWKEIAVL
HHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHH
FNSSLEAKTVQLPKEETWHVLVNEKQAKIEPISSFRGKELRLSPISTYILTIM
HCCCCCCEEEECCCCCCEEEEECCCCCCCCCHHHCCCCEEEECHHCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]