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The map label for this gene is megL [H]

Identifier: 218905884

GI number: 218905884

Start: 4519569

End: 4520747

Strand: Reverse

Name: megL [H]

Synonym: BCAH820_4772

Alternate gene names: 218905884

Gene position: 4520747-4519569 (Counterclockwise)

Preceding gene: 218905886

Following gene: 218905882

Centisome position: 85.25

GC content: 39.27

Gene sequence:

>1179_bases
ATGAAAAAGAAGCATATGGAGACAGCGTTAATTCATCACGGTTATACATCAGAGGAACATAAAGGAAGTTTAACACCGCC
TTTATTTCAAACGTCTACATTTACATTTGAGACTGCGCAGCAAGGAGAGGCGAGTTTTGCGGGAGTGGATCCATCTTATA
TTTACTCAAGACTTGGAAATCCGACTGTGAAATTATTTGAAGAACATATGGCGGTGTTAGAAGGTGGAGAAGAAGCTCTT
GCTTTCGGATCCGGAATGGCAGCTATTTCAGCAACTTTAATTGGTTTTCTAAAAGCTGGAGATCATATTATTTGTTCAAA
TGGATTATACGGATGCACGTACGGTTTTTTAGAAGTGTTAGAAGAAAAATTTATGATTACGCATTCGTTTTGTGATATGG
AGACAGAGGGTGATATTGAAAATAAAATTCGCCCAAATACAAAGCTTATTTTCGTTGAAACACCGATTAATCCGACAATG
AAATTAATTGATTTAAAACAAGTGATTCGGGTTGCGAAGCGAAATGGTTTACTTGTCATTGTTGACAATACGTTTTGTTC
ACCTTATTTACAAAGACCGCTTGAACTTGGCTGTGACGCCGTTGTTCATAGTGCGACAAAATATATTGGTGGTCACGGTG
ATGTTGTAGCGGGTGTAACAATTTGTAAAACGAAAGCGTTAGCTGAAAAAATTCGCCCGATGCGAAAAGATATCGGCGGT
ATTATGGCACCGTTTGATGCGTGGTTATTGTTACGCGGATTAAAGACACTAGCGGTAAGAATGGACCGCCATTGTGATAA
TGCAGAAAAAATTGTATCGTTCCTAAAAAATCATGATGCGGTAGAAGGTGTTTGGTATCCAGAAGGGGAGTTAGCATCTC
GCCAAATGAAACGTGGGGGCGGCGTTATATCTTTTTCAATTAAAGGCGGGAAAGAAGAGACGCAGGCGTTTATTAATGAC
CTTCACTTTATTACAATTGCGGTGAGTTTAGGAGATACAGAAACGTTAATTCAGCATCCGGCAACGATGACGCACGCTGC
GATTCCAGCTGAGTTAAGACAAGAAATGGGCATTTATGATAATTTAATACGTTTATCTGTCGGTTTAGAATCGTGGGAGG
ATATCGTTTCTGATTTAGAGCAGGCATTAAAGAAAATATCTACGGTTAGTAACCAATAA

Upstream 100 bases:

>100_bases
TAGAAACACAAATAAAACGCTTACATAAAATGTGGTAAAAACGTTCATCAGTTTTTTACTGGTGAATATGCGGTAATAAA
AACATAAAGGGGATGTTTTT

Downstream 100 bases:

>100_bases
AAAAAGAGCTAGCTATCGGTTTTGTACGTAATGTACTTAATCGATAACTAGTTCTTTTTTGGTTACATATGTTTTAGGAG
TGTTTCCACAAATTTTTCTA

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDPSYIYSRLGNPTVKLFEEHMAVLEGGEEAL
AFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVLEEKFMITHSFCDMETEGDIENKIRPNTKLIFVETPINPTM
KLIDLKQVIRVAKRNGLLVIVDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTKALAEKIRPMRKDIGG
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGGGVISFSIKGGKEETQAFIND
LHFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYDNLIRLSVGLESWEDIVSDLEQALKKISTVSNQ

Sequences:

>Translated_392_residues
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDPSYIYSRLGNPTVKLFEEHMAVLEGGEEAL
AFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVLEEKFMITHSFCDMETEGDIENKIRPNTKLIFVETPINPTM
KLIDLKQVIRVAKRNGLLVIVDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTKALAEKIRPMRKDIGG
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGGGVISFSIKGGKEETQAFIND
LHFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYDNLIRLSVGLESWEDIVSDLEQALKKISTVSNQ
>Mature_392_residues
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDPSYIYSRLGNPTVKLFEEHMAVLEGGEEAL
AFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVLEEKFMITHSFCDMETEGDIENKIRPNTKLIFVETPINPTM
KLIDLKQVIRVAKRNGLLVIVDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTKALAEKIRPMRKDIGG
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGGGVISFSIKGGKEETQAFIND
LHFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYDNLIRLSVGLESWEDIVSDLEQALKKISTVSNQ

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=389, Percent_Identity=40.6169665809769, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI299473759, Length=389, Percent_Identity=36.2467866323907, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI25453487, Length=388, Percent_Identity=35.5670103092784, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI1790375, Length=390, Percent_Identity=36.4102564102564, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1789383, Length=397, Percent_Identity=27.2040302267003, Blast_Score=172, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17560056, Length=390, Percent_Identity=40.2564102564103, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17538055, Length=390, Percent_Identity=41.2820512820513, Blast_Score=283, Evalue=8e-77,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=39.4594594594595, Blast_Score=264, Evalue=6e-71,
Organism=Caenorhabditis elegans, GI193202265, Length=88, Percent_Identity=37.5, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319307, Length=391, Percent_Identity=39.386189258312, Blast_Score=267, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6323333, Length=446, Percent_Identity=30.0448430493274, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321254, Length=413, Percent_Identity=30.2663438256659, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6321904, Length=400, Percent_Identity=30.25, Blast_Score=153, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6321170, Length=320, Percent_Identity=30.3125, Blast_Score=143, Evalue=6e-35,
Organism=Drosophila melanogaster, GI19922554, Length=385, Percent_Identity=38.1818181818182, Blast_Score=263, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 43191; Mature: 43191

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDPSYIYSRLGN
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCC
PTVKLFEEHMAVLEGGEEALAFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVL
HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
EEKFMITHSFCDMETEGDIENKIRPNTKLIFVETPINPTMKLIDLKQVIRVAKRNGLLVI
HHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEE
VDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTKALAEKIRPMRKDIGG
ECCCCCCHHHHCHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHCC
GVISFSIKGGKEETQAFINDLHFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYD
CEEEEEECCCHHHHHHHHHCCEEEEEEEECCCHHHHHHCCCHHHHHCCCHHHHHHCCHHH
NLIRLSVGLESWEDIVSDLEQALKKISTVSNQ
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDPSYIYSRLGN
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCC
PTVKLFEEHMAVLEGGEEALAFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVL
HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
EEKFMITHSFCDMETEGDIENKIRPNTKLIFVETPINPTMKLIDLKQVIRVAKRNGLLVI
HHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEE
VDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTKALAEKIRPMRKDIGG
ECCCCCCHHHHCHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHCC
GVISFSIKGGKEETQAFINDLHFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYD
CEEEEEECCCHHHHHHHHHCCEEEEEEEECCCHHHHHHCCCHHHHHCCCHHHHHHCCHHH
NLIRLSVGLESWEDIVSDLEQALKKISTVSNQ
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]