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The map label for this gene is nadB [H]

Identifier: 218905625

GI number: 218905625

Start: 4268070

End: 4269599

Strand: Reverse

Name: nadB [H]

Synonym: BCAH820_4510

Alternate gene names: 218905625

Gene position: 4269599-4268070 (Counterclockwise)

Preceding gene: 218905629

Following gene: 218905624

Centisome position: 80.52

GC content: 39.15

Gene sequence:

>1530_bases
ATGCCAAGTGCAGATGTCTTAATTATTGGAAGTGGTGTTGCGGCACTTCGTGTTGCAAAAGAGATTTGTCACGAAAAGAA
TGTGATTGTTATCACAAAGGAAACGAATCGTAATAATAATACACATTTAGCGCAAGGTGGAATTGCCGCAGCGGTTGCTA
CATATGACGATCCGAATGATCATTTTGAAGATACATTAGTAGCAGGATGTCATTATAACAATGAAGATGTGGTCCGTTAT
TTAGTTGAAGAAGGACCGAAAGAAATGAAGAATCTCATTGAAAATGGAATGCAATTTGATGGAGATGAAACGGGTCCCCA
TCTTGGAAAAGAAGGTGCACATCGAAAGCGTCGTATTTTACATGCAGGTGGCGATGCAACAGGAAAGAATTTATTAGAGC
ATTTCATTCAAGAAGTAGTTCCGCATGTTACGGTAGTGGAACAGGAAATGGTGCTTGATTTTATTATAGAAAGTGAAAAA
TGTGTTGGAGCTTTAACGCGAGATAGTGAAGGGAAGTTGAAGCGTTATGATGCAGATTATACTGTGCTAGCCTCAGGCGG
GGTTGGTGGTTTATACGCCTTTACTTCTAATGATAAAACAATTACGGGTGATGGACTTGCGATGGTATACCGCGCAGGTG
GGGAGCTCGTAGATTTAGAGTTTGTACAATTTCATCCAACAATGTTATATGCGGGCGGGCGATGCTGTGGCCTCGTTTCT
GAAGCTGTCCGTGGGGAAGGAGCTGTCCTTATAAATGGAAAAGGGCAGCGTTTTATGATGGATATACACGATGATTACGA
TCTCGCACCGCGTGATGTGGTGGCAAGAGCGATTCATGAACAGCTTTTGTCGGGTGAAAAAGTGTATTTGAATATTGAGT
CTATCCAAAATTTCCAAGAACGTTTTCCAACTGTATCAGCATTGTGTAAAAAGAATGGTGTTGATATTACTAAAAAACGC
ATTCCGGTCGTACCCGGAGCTCATTTTCATATGGGCGGTGTGAAAACAAACATTGATGGGGAGACGTCTATTCCAAATTT
ATATGCAGTCGGTGAAGTAGCTTGTAATGGTGTTCATGGAGCAAATAGATTAGCGAGTAATTCGTTATTAGAAGGTCTTG
TGTTTGGAAAAAGAATAGGACGACATATTTTAACGAAAGCGACAAAAGACAAATTGAACGACTTCGCTGAGAAGGAAAAG
AAATTTATCGTTCTAAATCATTTGCCGACGAAAGAAGAAATACAAGAATGCATGATGAAATATGTTGGGATTGTACGAAC
AGAGCAAAGTTTATCTTATGCGAAGAGATGGCTTAGTAAATACGGTGTTCGAAATATGATATTACAACATGATGCTCTTA
CAAATGAAGAGATAACGCTTATTAATATGTTAACAGTTTGTGAGCTTATCGTTGTATCAGCTTTACAAAGAGAAGAGAGT
ATTGGTGGTCATTATCGAAGTGATTATCCACATAGAAATAGCGTAAAAAAAGAGATCATTCGAGTGAAAAGAAAACTACA
ACTTGTGTAA

Upstream 100 bases:

>100_bases
TTGAAAATAAGAATTATWAAAAAACTCTTGTCATTATTTTAAAACTATGTAAATATAGGTGTCAAGACAGTTGAAAAGTG
TAAAGTAGGAGGCAACAATT

Downstream 100 bases:

>100_bases
TGAGGGGAAGAGGGGCTTTATGAATACGATAAAAGTGAAAGAAGCATTAAATCGATTTTTTCTAGAAGATATAGGAGAAA
GAGATGTAACATCTCAGCTT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MPSADVLIIGSGVAALRVAKEICHEKNVIVITKETNRNNNTHLAQGGIAAAVATYDDPNDHFEDTLVAGCHYNNEDVVRY
LVEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRILHAGGDATGKNLLEHFIQEVVPHVTVVEQEMVLDFIIESEK
CVGALTRDSEGKLKRYDADYTVLASGGVGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGRCCGLVS
EAVRGEGAVLINGKGQRFMMDIHDDYDLAPRDVVARAIHEQLLSGEKVYLNIESIQNFQERFPTVSALCKKNGVDITKKR
IPVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHGANRLASNSLLEGLVFGKRIGRHILTKATKDKLNDFAEKEK
KFIVLNHLPTKEEIQECMMKYVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREES
IGGHYRSDYPHRNSVKKEIIRVKRKLQLV

Sequences:

>Translated_509_residues
MPSADVLIIGSGVAALRVAKEICHEKNVIVITKETNRNNNTHLAQGGIAAAVATYDDPNDHFEDTLVAGCHYNNEDVVRY
LVEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRILHAGGDATGKNLLEHFIQEVVPHVTVVEQEMVLDFIIESEK
CVGALTRDSEGKLKRYDADYTVLASGGVGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGRCCGLVS
EAVRGEGAVLINGKGQRFMMDIHDDYDLAPRDVVARAIHEQLLSGEKVYLNIESIQNFQERFPTVSALCKKNGVDITKKR
IPVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHGANRLASNSLLEGLVFGKRIGRHILTKATKDKLNDFAEKEK
KFIVLNHLPTKEEIQECMMKYVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREES
IGGHYRSDYPHRNSVKKEIIRVKRKLQLV
>Mature_508_residues
PSADVLIIGSGVAALRVAKEICHEKNVIVITKETNRNNNTHLAQGGIAAAVATYDDPNDHFEDTLVAGCHYNNEDVVRYL
VEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRILHAGGDATGKNLLEHFIQEVVPHVTVVEQEMVLDFIIESEKC
VGALTRDSEGKLKRYDADYTVLASGGVGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGRCCGLVSE
AVRGEGAVLINGKGQRFMMDIHDDYDLAPRDVVARAIHEQLLSGEKVYLNIESIQNFQERFPTVSALCKKNGVDITKKRI
PVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHGANRLASNSLLEGLVFGKRIGRHILTKATKDKLNDFAEKEKK
FIVLNHLPTKEEIQECMMKYVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREESI
GGHYRSDYPHRNSVKKEIIRVKRKLQLV

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=524, Percent_Identity=33.3969465648855, Blast_Score=214, Evalue=2e-55,
Organism=Escherichia coli, GI1788928, Length=516, Percent_Identity=36.4341085271318, Blast_Score=292, Evalue=3e-80,
Organism=Escherichia coli, GI1790597, Length=511, Percent_Identity=31.8982387475538, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1786942, Length=522, Percent_Identity=30.2681992337165, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17550100, Length=522, Percent_Identity=31.992337164751, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17505833, Length=524, Percent_Identity=31.8702290076336, Blast_Score=212, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI71986328, Length=462, Percent_Identity=25.7575757575758, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322701, Length=528, Percent_Identity=33.7121212121212, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6322416, Length=517, Percent_Identity=33.6557059961315, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6320788, Length=480, Percent_Identity=26.0416666666667, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322511, Length=473, Percent_Identity=25.7928118393235, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI17137288, Length=522, Percent_Identity=34.2911877394636, Blast_Score=218, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24655642, Length=522, Percent_Identity=34.2911877394636, Blast_Score=218, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24655647, Length=522, Percent_Identity=34.2911877394636, Blast_Score=218, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24663005, Length=523, Percent_Identity=31.1663479923518, Blast_Score=205, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 56361; Mature: 56230

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00079 MULTICOPPER_OXIDASE1 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSADVLIIGSGVAALRVAKEICHEKNVIVITKETNRNNNTHLAQGGIAAAVATYDDPND
CCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCEEEEEEECCCCCC
HFEDTLVAGCHYNNEDVVRYLVEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRIL
HHHHHEEECCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHE
HAGGDATGKNLLEHFIQEVVPHVTVVEQEMVLDFIIESEKCVGALTRDSEGKLKRYDADY
ECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEECCCE
TVLASGGVGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGRCCGLVS
EEEECCCCCEEEEEECCCCEECCCCEEEEEECCCCEEEEEEEEECCEEEECCCCHHHHHH
EAVRGEGAVLINGKGQRFMMDIHDDYDLAPRDVVARAIHEQLLSGEKVYLNIESIQNFQE
HHHCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHH
RFPTVSALCKKNGVDITKKRIPVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHG
HCCHHHHHHHCCCCCCHHHCCCCCCCCCEECCCEEECCCCCCCCCCEEEEHHHHHCCCCC
ANRLASNSLLEGLVFGKRIGRHILTKATKDKLNDFAEKEKKFIVLNHLPTKEEIQECMMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHH
YVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREES
HHHHHCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGGHYRSDYPHRNSVKKEIIRVKRKLQLV
CCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSADVLIIGSGVAALRVAKEICHEKNVIVITKETNRNNNTHLAQGGIAAAVATYDDPND
CCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCEEEEEEECCCCCC
HFEDTLVAGCHYNNEDVVRYLVEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRIL
HHHHHEEECCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHE
HAGGDATGKNLLEHFIQEVVPHVTVVEQEMVLDFIIESEKCVGALTRDSEGKLKRYDADY
ECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEECCCE
TVLASGGVGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGRCCGLVS
EEEECCCCCEEEEEECCCCEECCCCEEEEEECCCCEEEEEEEEECCEEEECCCCHHHHHH
EAVRGEGAVLINGKGQRFMMDIHDDYDLAPRDVVARAIHEQLLSGEKVYLNIESIQNFQE
HHHCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHH
RFPTVSALCKKNGVDITKKRIPVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHG
HCCHHHHHHHCCCCCCHHHCCCCCCCCCEECCCEEECCCCCCCCCCEEEEHHHHHCCCCC
ANRLASNSLLEGLVFGKRIGRHILTKATKDKLNDFAEKEKKFIVLNHLPTKEEIQECMMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHH
YVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREES
HHHHHCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGGHYRSDYPHRNSVKKEIIRVKRKLQLV
CCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8444804; 9384377 [H]