Click here to switch to the map view.

The map label for this gene is hisS [H]

Identifier: 218905598

GI number: 218905598

Start: 4239664

End: 4240935

Strand: Reverse

Name: hisS [H]

Synonym: BCAH820_4483

Alternate gene names: 218905598

Gene position: 4240935-4239664 (Counterclockwise)

Preceding gene: 218905599

Following gene: 218905597

Centisome position: 79.98

GC content: 40.25

Gene sequence:

>1272_bases
ATGTCTATTCAAATCCCACGCGGAACGCAAGATATTCTTCCAGGCACTGTTGAGTTATGGCAGTATATCGAAGGGCAAGC
ACGCGAAATTTGCCGTCGTTACAATTATAAAGAAATTCGTACACCGATCTTTGAACACACTGAGTTATTTTTACGTGGTG
TTGGTGATACGACAGATATCGTGCAAAAAGAAATGTACTCATTCCAAGATCGTGGAGAGCGTAGTTTAACATTACGTCCA
GAAGGCACTGCACCTGTTGTACGTTCTTACGTTGAAAACAAAATGTTCGGTGACGCAACACAACCAACGAAATTATATTA
TATCGGTCAAATGTTCCGTTACGAAAGACCACAAGCAGGTCGCTATCGTCAATTCGTACAATTCGGTATTGAAGCAATCG
GTAGTAACGATCCTGCAATTGATGCGGAAGTAATTGCACTTGCTGTAGAGTTTTACCGCGGCATGGGCTTAAAAAATATT
AAAGTTGTATTAAACAGCTTAGGTGATGCGGCGAGCCGTCAAGCGCACCGTGATGCGTTAATTGCACACTTTGAGCCACG
TATCGGTGAGTTCTGTTCTGACTGTCAATCTCGTTTAGAAAAGAACCCTCTTCGTATTTTAGATTGTAAGAAGGACCGTA
ACCATGAATTAATGGGAACAGCACCATCTATTACAGAATACTTAAACGAAGATTCAGCAGTATACTACGACAAAGTTCAA
GAACTATTAACGATGATGGATGTTCCATTTGAAAAAGATCCGAACTTAGTACGTGGTTTAGACTACTACCAGCACACGGT
TTTTGAAATTATGAGTGAAGCAGAAGGTTTCGGTGCGATCACTACACTAAGCGGTGGTGGCCGTTATAACGGACTTGTAA
AAGAAATCGGTGGACCAGAAATGCCAGGTATCGGTTTTGCGATGAGTATTGAACGTTTAATTATGGCGCTAAAAGCTGAA
AACATCGAATTACCAATTGAACATAGTATCGATTGTTACGTTGTAGCGCTTGGTGAAAAAGCGAAAGACCATGCTGCAAA
AGTTGCGTTTGATCTTCGTAAAGCTGGATTAGCAGTTGAAAAAGATTATTTAGATCGCAAAATGAAAGCACAATTTAAAT
CAGCAGATCGTCTAAAAGCGAAATTCGTAGCTGTACTAGGGGAAGATGAGCTAGATAAAGGCATCATTAACTTAAAAGAT
ATGGCAACAGGCGAACAAGAAGAAGTAGCATTAGATGTGTTTGCTTCATACGTAGCAGAGAAATTAATATAG

Upstream 100 bases:

>100_bases
GCACCACGGGTATAATACTCTCGTCCCTACTGTTCAAACAGTAGAGGCGGGAGTTTTTTTATTTTTATTAAGATTAAAAC
AATTTGAGGAGGAACTGAAT

Downstream 100 bases:

>100_bases
GGGGAACTTACAGTGGCTGAAAGAACACATGCATGTGGAAAAGTAACAGTAGAAGCAGTTGGACAAACAGTTCAATTAAA
AGGTTGGGTACAAAAACGCC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase 2; HisRS 2 [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSIQIPRGTQDILPGTVELWQYIEGQAREICRRYNYKEIRTPIFEHTELFLRGVGDTTDIVQKEMYSFQDRGERSLTLRP
EGTAPVVRSYVENKMFGDATQPTKLYYIGQMFRYERPQAGRYRQFVQFGIEAIGSNDPAIDAEVIALAVEFYRGMGLKNI
KVVLNSLGDAASRQAHRDALIAHFEPRIGEFCSDCQSRLEKNPLRILDCKKDRNHELMGTAPSITEYLNEDSAVYYDKVQ
ELLTMMDVPFEKDPNLVRGLDYYQHTVFEIMSEAEGFGAITTLSGGGRYNGLVKEIGGPEMPGIGFAMSIERLIMALKAE
NIELPIEHSIDCYVVALGEKAKDHAAKVAFDLRKAGLAVEKDYLDRKMKAQFKSADRLKAKFVAVLGEDELDKGIINLKD
MATGEQEEVALDVFASYVAEKLI

Sequences:

>Translated_423_residues
MSIQIPRGTQDILPGTVELWQYIEGQAREICRRYNYKEIRTPIFEHTELFLRGVGDTTDIVQKEMYSFQDRGERSLTLRP
EGTAPVVRSYVENKMFGDATQPTKLYYIGQMFRYERPQAGRYRQFVQFGIEAIGSNDPAIDAEVIALAVEFYRGMGLKNI
KVVLNSLGDAASRQAHRDALIAHFEPRIGEFCSDCQSRLEKNPLRILDCKKDRNHELMGTAPSITEYLNEDSAVYYDKVQ
ELLTMMDVPFEKDPNLVRGLDYYQHTVFEIMSEAEGFGAITTLSGGGRYNGLVKEIGGPEMPGIGFAMSIERLIMALKAE
NIELPIEHSIDCYVVALGEKAKDHAAKVAFDLRKAGLAVEKDYLDRKMKAQFKSADRLKAKFVAVLGEDELDKGIINLKD
MATGEQEEVALDVFASYVAEKLI
>Mature_422_residues
SIQIPRGTQDILPGTVELWQYIEGQAREICRRYNYKEIRTPIFEHTELFLRGVGDTTDIVQKEMYSFQDRGERSLTLRPE
GTAPVVRSYVENKMFGDATQPTKLYYIGQMFRYERPQAGRYRQFVQFGIEAIGSNDPAIDAEVIALAVEFYRGMGLKNIK
VVLNSLGDAASRQAHRDALIAHFEPRIGEFCSDCQSRLEKNPLRILDCKKDRNHELMGTAPSITEYLNEDSAVYYDKVQE
LLTMMDVPFEKDPNLVRGLDYYQHTVFEIMSEAEGFGAITTLSGGGRYNGLVKEIGGPEMPGIGFAMSIERLIMALKAEN
IELPIEHSIDCYVVALGEKAKDHAAKVAFDLRKAGLAVEKDYLDRKMKAQFKSADRLKAKFVAVLGEDELDKGIINLKDM
ATGEQEEVALDVFASYVAEKLI

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=472, Percent_Identity=24.364406779661, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI6996014, Length=454, Percent_Identity=25.1101321585903, Blast_Score=106, Evalue=4e-23,
Organism=Escherichia coli, GI1788861, Length=420, Percent_Identity=41.6666666666667, Blast_Score=337, Evalue=7e-94,
Organism=Caenorhabditis elegans, GI71993686, Length=477, Percent_Identity=24.1090146750524, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71993693, Length=477, Percent_Identity=24.1090146750524, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6325290, Length=477, Percent_Identity=23.4800838574423, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24643061, Length=476, Percent_Identity=24.7899159663866, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24643059, Length=495, Percent_Identity=23.8383838383838, Blast_Score=108, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 47747; Mature: 47616

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQIPRGTQDILPGTVELWQYIEGQAREICRRYNYKEIRTPIFEHTELFLRGVGDTTDI
CEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCHHHH
VQKEMYSFQDRGERSLTLRPEGTAPVVRSYVENKMFGDATQPTKLYYIGQMFRYERPQAG
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHCCCCCCH
RYRQFVQFGIEAIGSNDPAIDAEVIALAVEFYRGMGLKNIKVVLNSLGDAASRQAHRDAL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
IAHFEPRIGEFCSDCQSRLEKNPLRILDCKKDRNHELMGTAPSITEYLNEDSAVYYDKVQ
HHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHH
ELLTMMDVPFEKDPNLVRGLDYYQHTVFEIMSEAEGFGAITTLSGGGRYNGLVKEIGGPE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHCCCCC
MPGIGFAMSIERLIMALKAENIELPIEHSIDCYVVALGEKAKDHAAKVAFDLRKAGLAVE
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCHHH
KDYLDRKMKAQFKSADRLKAKFVAVLGEDELDKGIINLKDMATGEQEEVALDVFASYVAE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHCCCCHHHHHHHHHHHHHHH
KLI
HCC
>Mature Secondary Structure 
SIQIPRGTQDILPGTVELWQYIEGQAREICRRYNYKEIRTPIFEHTELFLRGVGDTTDI
EEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCHHHH
VQKEMYSFQDRGERSLTLRPEGTAPVVRSYVENKMFGDATQPTKLYYIGQMFRYERPQAG
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHCCCCCCH
RYRQFVQFGIEAIGSNDPAIDAEVIALAVEFYRGMGLKNIKVVLNSLGDAASRQAHRDAL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
IAHFEPRIGEFCSDCQSRLEKNPLRILDCKKDRNHELMGTAPSITEYLNEDSAVYYDKVQ
HHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHH
ELLTMMDVPFEKDPNLVRGLDYYQHTVFEIMSEAEGFGAITTLSGGGRYNGLVKEIGGPE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHCCCCC
MPGIGFAMSIERLIMALKAENIELPIEHSIDCYVVALGEKAKDHAAKVAFDLRKAGLAVE
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCHHH
KDYLDRKMKAQFKSADRLKAKFVAVLGEDELDKGIINLKDMATGEQEEVALDVFASYVAE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHCCCCHHHHHHHHHHHHHHH
KLI
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]