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The map label for this gene is sigK [H]

Identifier: 218905533

GI number: 218905533

Start: 4180158

End: 4180607

Strand: Reverse

Name: sigK [H]

Synonym: BCAH820_4418

Alternate gene names: 218905533

Gene position: 4180607-4180158 (Counterclockwise)

Preceding gene: 218905535

Following gene: 218905528

Centisome position: 78.84

GC content: 38.22

Gene sequence:

>450_bases
TTGAGTCTATTCGCCGCAATTGGATATATGGTTCGAGAAGTGTTTGTTTTTGTTTCTTATGTGAAGAACAATGCGTTTCC
GCAGCCATTATCATCAGACGATGAGAGAAAGTACTTAGAGTTAATGGAGCAAGGTGATGCTCAAGCGAGGAATCTGTTAA
TTGAACATAATTTACGGCTTGTAGCTCATATCGTTAAAAAATTTGAAAATACAGGGGAAGATGCAGAAGATTTAATTTCA
ATTGGTACAATCGGGCTCATTAAAGCAATCGAGAGCTATTCGGCAGGAAAAGGTACAAAACTTGCGACGTACGCAGCACG
TTGTATTGAAAATGAAATTTTGATGCATTTACGTGTATTAAAGAAAACGAAAAAGGACGTTTCACTTCATGATCCAATCG
GGCAAGATAAAGAGGGGAATGAGATGCCGACTAGACTTTATCCTGTTTAG

Upstream 100 bases:

>100_bases
ATTGGAAGTGTTGAGTTTTATAAATAAACTGTACAGACACAAACAACCTCACGCCCACATACACTTAGTAAAGACCACTA
TTTTGGCGGAGGTGAAAGGT

Downstream 100 bases:

>100_bases
AAAAAGCCCGTAGAATAGGGGTCTACAGGCTTACTTTGGTAACTTAGGATATAGAGTTAGAGAAAAATCATCCTTGTATT
GATGTTTCTCTTTATTATAA

Product: RNA polymerase sigma-K factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 148

Protein sequence:

>149_residues
MSLFAAIGYMVREVFVFVSYVKNNAFPQPLSSDDERKYLELMEQGDAQARNLLIEHNLRLVAHIVKKFENTGEDAEDLIS
IGTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRVLKKTKKDVSLHDPIGQDKEGNEMPTRLYPV

Sequences:

>Translated_149_residues
MSLFAAIGYMVREVFVFVSYVKNNAFPQPLSSDDERKYLELMEQGDAQARNLLIEHNLRLVAHIVKKFENTGEDAEDLIS
IGTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRVLKKTKKDVSLHDPIGQDKEGNEMPTRLYPV
>Mature_148_residues
SLFAAIGYMVREVFVFVSYVKNNAFPQPLSSDDERKYLELMEQGDAQARNLLIEHNLRLVAHIVKKFENTGEDAEDLISI
GTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRVLKKTKKDVSLHDPIGQDKEGNEMPTRLYPV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor directs the transcription of crystal protein genes, a sporulation-regulated event [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=109, Percent_Identity=37.6146788990826, Blast_Score=62, Evalue=1e-11,
Organism=Escherichia coli, GI1789098, Length=106, Percent_Identity=31.1320754716981, Blast_Score=59, Evalue=9e-11,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014209
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 16785; Mature: 16653

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFAAIGYMVREVFVFVSYVKNNAFPQPLSSDDERKYLELMEQGDAQARNLLIEHNLRL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
VAHIVKKFENTGEDAEDLISIGTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRVL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KKTKKDVSLHDPIGQDKEGNEMPTRLYPV
HHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLFAAIGYMVREVFVFVSYVKNNAFPQPLSSDDERKYLELMEQGDAQARNLLIEHNLRL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
VAHIVKKFENTGEDAEDLISIGTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRVL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KKTKKDVSLHDPIGQDKEGNEMPTRLYPV
HHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]