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The map label for this gene is scrFIBM [H]

Identifier: 218905512

GI number: 218905512

Start: 4167998

End: 4168972

Strand: Reverse

Name: scrFIBM [H]

Synonym: BCAH820_4397

Alternate gene names: 218905512

Gene position: 4168972-4167998 (Counterclockwise)

Preceding gene: 218905513

Following gene: 218905511

Centisome position: 78.62

GC content: 38.36

Gene sequence:

>975_bases
TTGATTAAAATACTTGAATTGTTCGGAGGCATCGGCGCACCAAGAAAGGCACTTGTTAATTTAGGAATAGATCATAAAGC
AATTGATTATGTAGAATGGCAAGCTAATAGAGTAAAAGCATATAACGCTCTATATGACCACCTACATAAGCCACAGGATG
TAAGAGGATGGAATTTAAAGCCGGATATTTTAGTTCACGGGTCACCTTGCCAAGATAACAGTAGAGCGAATAAAGCACGT
AAAGGTAATGCTGAAGATTCGCGCTCTCAATTGATGTTGGAAACGCTACGGATAATTAAAGAAATGGGTGAGTGGCGACC
TAAGGTTATCATTTGGGAAAACGTGAAAGGTGTGCTGGATCGTAATGTAGTACCAGTATTTAATTATTACCTGGAAGAAA
TGGAGAAGCTAGGTTATACAAATTCGTATGATGTATTGGACGCTAGGAAATTCGGAGTGCCACATGCAAGAGAAAGAGTA
TTTTGTATCTCGTTATTAGGTGGCAAAGCGTTTGATTTTAATAAATTGAGATTTAAACCAATGAGGGATATTAAAGAATT
TATGGAGTATGGACCAGATGATAAAATTCCATCGCAGTATTTAATCAATATTCCATCGATGCTGAACAAAATTAAAGAGT
TCAATCCAAATCCAACAGGTAGTTATAAACGGCAGCTAGATGTAATTGATCAGTATTGCTACACAATAACCGAAAGACAA
GATCGTTGCCCCAATGCAGGGATTATCCGATTGAGCGAACCGCGGTACCGGTATTTAACAGAACGTGAGTGTTGGAGATT
ATTAGGATTCGGTGATGAAGATTACGAGCTTATGCTAAAAGAGTTTCCAACAAAGCCAGGGAAGCGAAATGCAACGTTAT
ATGCATTAGCTGGTAACAGTATAGTAGTTGATGTTTTAGAAGCAATCTTTGAAGTGCTACTTGATATGCTGCAGGGGCAG
TTGCAAGCAAGTTAA

Upstream 100 bases:

>100_bases
AATAATTTTGGCGGATGATATGAAGTACTGTGTTGAAAATAACCTAACGTTAGAATTTATATAAAAACGCTATTTTAATA
GCAAGAAGGGAGGGGTGGCT

Downstream 100 bases:

>100_bases
TTATAAAAATTTCATTTTAATAAAAAACAAGGAGGAAGTTAATTATGAGTCATTTTGCGGTTATGGTACAAATTCCAAAA
GAAAAAGCTGGGGATAGAAA

Product: modification methylase ScrFIB

Products: NA

Alternate protein names: M.ScrFI-B; M.ScrFIB; Cytosine-specific methyltransferase ScrFIB [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MIKILELFGGIGAPRKALVNLGIDHKAIDYVEWQANRVKAYNALYDHLHKPQDVRGWNLKPDILVHGSPCQDNSRANKAR
KGNAEDSRSQLMLETLRIIKEMGEWRPKVIIWENVKGVLDRNVVPVFNYYLEEMEKLGYTNSYDVLDARKFGVPHARERV
FCISLLGGKAFDFNKLRFKPMRDIKEFMEYGPDDKIPSQYLINIPSMLNKIKEFNPNPTGSYKRQLDVIDQYCYTITERQ
DRCPNAGIIRLSEPRYRYLTERECWRLLGFGDEDYELMLKEFPTKPGKRNATLYALAGNSIVVDVLEAIFEVLLDMLQGQ
LQAS

Sequences:

>Translated_324_residues
MIKILELFGGIGAPRKALVNLGIDHKAIDYVEWQANRVKAYNALYDHLHKPQDVRGWNLKPDILVHGSPCQDNSRANKAR
KGNAEDSRSQLMLETLRIIKEMGEWRPKVIIWENVKGVLDRNVVPVFNYYLEEMEKLGYTNSYDVLDARKFGVPHARERV
FCISLLGGKAFDFNKLRFKPMRDIKEFMEYGPDDKIPSQYLINIPSMLNKIKEFNPNPTGSYKRQLDVIDQYCYTITERQ
DRCPNAGIIRLSEPRYRYLTERECWRLLGFGDEDYELMLKEFPTKPGKRNATLYALAGNSIVVDVLEAIFEVLLDMLQGQ
LQAS
>Mature_324_residues
MIKILELFGGIGAPRKALVNLGIDHKAIDYVEWQANRVKAYNALYDHLHKPQDVRGWNLKPDILVHGSPCQDNSRANKAR
KGNAEDSRSQLMLETLRIIKEMGEWRPKVIIWENVKGVLDRNVVPVFNYYLEEMEKLGYTNSYDVLDARKFGVPHARERV
FCISLLGGKAFDFNKLRFKPMRDIKEFMEYGPDDKIPSQYLINIPSMLNKIKEFNPNPTGSYKRQLDVIDQYCYTITERQ
DRCPNAGIIRLSEPRYRYLTERECWRLLGFGDEDYELMLKEFPTKPGKRNATLYALAGNSIVVDVLEAIFEVLLDMLQGQ
LQAS

Specific function: This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ScrFI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 37544; Mature: 37544

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKILELFGGIGAPRKALVNLGIDHKAIDYVEWQANRVKAYNALYDHLHKPQDVRGWNLK
CEEHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PDILVHGSPCQDNSRANKARKGNAEDSRSQLMLETLRIIKEMGEWRPKVIIWENVKGVLD
CCEEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHC
RNVVPVFNYYLEEMEKLGYTNSYDVLDARKFGVPHARERVFCISLLGGKAFDFNKLRFKP
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHEEEEEECCCCCCCCCCCCCCH
MRDIKEFMEYGPDDKIPSQYLINIPSMLNKIKEFNPNPTGSYKRQLDVIDQYCYTITERQ
HHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
DRCPNAGIIRLSEPRYRYLTERECWRLLGFGDEDYELMLKEFPTKPGKRNATLYALAGNS
HCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCC
IVVDVLEAIFEVLLDMLQGQLQAS
HHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKILELFGGIGAPRKALVNLGIDHKAIDYVEWQANRVKAYNALYDHLHKPQDVRGWNLK
CEEHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PDILVHGSPCQDNSRANKARKGNAEDSRSQLMLETLRIIKEMGEWRPKVIIWENVKGVLD
CCEEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHC
RNVVPVFNYYLEEMEKLGYTNSYDVLDARKFGVPHARERVFCISLLGGKAFDFNKLRFKP
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHEEEEEECCCCCCCCCCCCCCH
MRDIKEFMEYGPDDKIPSQYLINIPSMLNKIKEFNPNPTGSYKRQLDVIDQYCYTITERQ
HHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
DRCPNAGIIRLSEPRYRYLTERECWRLLGFGDEDYELMLKEFPTKPGKRNATLYALAGNS
HCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCC
IVVDVLEAIFEVLLDMLQGQLQAS
HHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA