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The map label for this gene is miaB

Identifier: 218904900

GI number: 218904900

Start: 3615998

End: 3617527

Strand: Reverse

Name: miaB

Synonym: BCAH820_3784

Alternate gene names: 218904900

Gene position: 3617527-3615998 (Counterclockwise)

Preceding gene: 218904901

Following gene: 218904899

Centisome position: 68.22

GC content: 36.8

Gene sequence:

>1530_bases
ATGAACGAGCAACAACGATTAGCAAGTCAACAAGTGAATTCCTCTACGAAAAAAGAAGAGAAAGATTATAGCAAATACTT
TGAAAGTGTATACCAACCACCTTCCTTAAAAGACGCGAAAAAACGAGGGAAAGAAGAAGTTAAAATTGAACGGGATTTTG
GCTTGCCTGAAGAGTTTCGCAATTTTGGTACAGGAAGAAAGTTTTATATTCGTACATACGGTTGTCAAATGAATGAGCAT
GATACAGAAGTAATGGCGGGTATTTTCACTGCACTGGGATATGAACCAACCTTTTCAACTGAAGACGCAGATGTAGTATT
ATTAAATACTTGTGCCATTCGTGAAAATGCTGAAAATAAAGTGTTCGGTGAACTAGGTCATTTAAAGTCACTAAAACGAA
GAAATCCGGACCTTTTAATTGGTGTATGTGGTTGTATGTCTCAAGAGGAATCTGTTGTAAATAAAATTATGCAAAAAAAT
CAGCATGTAGATATGGTGTTTGGAACGCATAATATTCATAGATTACCGTACATTCTAAAAGATGCAATGTTCTCGAAGGA
GACGGTAGTTGAAGTTTGGTCCAAAGAAGGAGACGTAATTGAAAACCTTCCGAAAGTACGCCGTGGTGATATTAAAGCTT
GGGTAAATATTATGTATGGCTGTGATAAGTTCTGTACATATTGTATCGTACCGTATACACGCGGAAAAGAGCGAAGCAGA
CGTCCAGAAGACATTATCCAAGAGATTCGTCATTTAGCTGCGAATGGTTATAAAGAAATTACGTTACTTGGACAGAACGT
AAATGCATATGGTAAAGACTTTGAAGATATAGAATACGGTCTTGGCGATTTAATGGACGAACTCCGTAAAGTTGATATTG
CCCGTATTCGTTTTACAACGAGCCATCCACGCGATTTCGATGATCACTTAATTGAAGTGCTTGGAAAAGGCGGAAACTTA
GTAGAACATATTCACTTACCAGTTCAATCTGGAAGCACAGAAATGTTAAAAATTATGGCGCGTAAATATTCACGTGAGCA
TTATTTAGAGCTTGTAAGAAAAATTAAAGAAGCAATTCCAAACGCGGTATTAACAACTGATATTATCGTCGGTTTCCCGA
ATGAAACAGATGAGCAATTTGAAGAAACGATGTCGTTGTATCGTGAAGTAGGATTTGATACTGCCTTTACCTTTATTTAT
TCTCCGCGTGAAGGTACACCAGCTGCAAAAATGAAAGACAATGTACCGATGGAAGTAAAGAAAGAACGTCTTCAACGCTT
AAATGCATTAGTAAATAAATTGGCAATTGAAAAGAATGATCGTTATAAAGGTCAAATTGTAGAAGTGTTAGTTGATGGGG
AAAGTAAAAATAATCCAGAAGTACTTGCAGGTTATACTCGTACGAATAAACTTGTAAACTTCGTTGCTCCAAAGTCTTTA
ATTGGTCAGCTTGTGAAAGTAAAAGTAACGGATGCAAAAACTTGGTCTCTTAACGGAGAATTAGTTGAAGAGCCGATTGA
GGTGGAATAA

Upstream 100 bases:

>100_bases
ACAAGTATCTCTCAAGCTATCTAACCATTTTTGAGGTGGGTCTAAGCGCATTAAAATGGACTATAGAGTGTGTATAGTTA
AGGGTGAAAGGAGATTACCG

Downstream 100 bases:

>100_bases
TAGATGAAAGTATATTCGAAAGATGAAATTGTTGAGCAAGCGAAAGAATTAGCAAAAATGATTTCTGAAACAGAAGAAGT
TGATTTCTTTAAGAAAGCAG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKDAKKRGKEEVKIERDFGLPEEFRNFGTGRKFYIRTYGCQMNEH
DTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELGHLKSLKRRNPDLLIGVCGCMSQEESVVNKIMQKN
QHVDMVFGTHNIHRLPYILKDAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTTSHPRDFDDHLIEVLGKGGNL
VEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIPNAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIY
SPREGTPAAKMKDNVPMEVKKERLQRLNALVNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
IGQLVKVKVTDAKTWSLNGELVEEPIEVE

Sequences:

>Translated_509_residues
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKDAKKRGKEEVKIERDFGLPEEFRNFGTGRKFYIRTYGCQMNEH
DTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELGHLKSLKRRNPDLLIGVCGCMSQEESVVNKIMQKN
QHVDMVFGTHNIHRLPYILKDAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTTSHPRDFDDHLIEVLGKGGNL
VEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIPNAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIY
SPREGTPAAKMKDNVPMEVKKERLQRLNALVNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
IGQLVKVKVTDAKTWSLNGELVEEPIEVE
>Mature_509_residues
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKDAKKRGKEEVKIERDFGLPEEFRNFGTGRKFYIRTYGCQMNEH
DTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELGHLKSLKRRNPDLLIGVCGCMSQEESVVNKIMQKN
QHVDMVFGTHNIHRLPYILKDAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTTSHPRDFDDHLIEVLGKGGNL
VEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIPNAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIY
SPREGTPAAKMKDNVPMEVKKERLQRLNALVNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
IGQLVKVKVTDAKTWSLNGELVEEPIEVE

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=483, Percent_Identity=37.2670807453416, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI28872784, Length=483, Percent_Identity=37.2670807453416, Blast_Score=306, Evalue=3e-83,
Organism=Homo sapiens, GI93277076, Length=455, Percent_Identity=29.010989010989, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1786882, Length=440, Percent_Identity=45, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1787057, Length=454, Percent_Identity=27.3127753303965, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=480, Percent_Identity=35.4166666666667, Blast_Score=272, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI71996771, Length=307, Percent_Identity=31.2703583061889, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI21356207, Length=550, Percent_Identity=34.5454545454545, Blast_Score=298, Evalue=7e-81,
Organism=Drosophila melanogaster, GI19922432, Length=457, Percent_Identity=30.8533916849015, Blast_Score=193, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_BACAH (A0RHE4)

Other databases:

- EMBL:   CP000485
- RefSeq:   YP_896144.1
- STRING:   A0RHE4
- EnsemblBacteria:   EBBACT00000071011
- GeneID:   4545208
- GenomeReviews:   CP000485_GR
- KEGG:   btl:BALH_3400
- NMPDR:   fig|412694.5.peg.3267
- eggNOG:   COG0621
- GeneTree:   EBGT00050000002619
- HOGENOM:   HBG457663
- ProtClustDB:   PRK14327
- BioCyc:   BTHU412694:BALH_3400-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 58344; Mature: 58344

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKDAKKRGKEEVKIERDFGLPEEFR
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHEEEECCCCCCHHHH
NFGTGRKFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENK
HCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCCCCCH
VFGELGHLKSLKRRNPDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILK
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
DAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
HHHHCHHHHHHHHHCCCCHHHHCHHHHHCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCC
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTT
CHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEEEC
SHPRDFDDHLIEVLGKGGNLVEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIP
CCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCC
NAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVK
CCEEEEHEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCHHHH
KERLQRLNALVNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHCCCHHH
IGQLVKVKVTDAKTWSLNGELVEEPIEVE
HHHHHEEEEECCEEEECCCHHHCCCCCCC
>Mature Secondary Structure
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKDAKKRGKEEVKIERDFGLPEEFR
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHEEEECCCCCCHHHH
NFGTGRKFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENK
HCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCCCCCH
VFGELGHLKSLKRRNPDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILK
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
DAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
HHHHCHHHHHHHHHCCCCHHHHCHHHHHCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCC
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTT
CHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEEEC
SHPRDFDDHLIEVLGKGGNLVEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIP
CCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCC
NAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVK
CCEEEEHEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCHHHH
KERLQRLNALVNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHCCCHHH
IGQLVKVKVTDAKTWSLNGELVEEPIEVE
HHHHHEEEEECCEEEECCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA