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The map label for this gene is miaA [H]

Identifier: 218904839

GI number: 218904839

Start: 3555626

End: 3556579

Strand: Reverse

Name: miaA [H]

Synonym: BCAH820_3723

Alternate gene names: 218904839

Gene position: 3556579-3555626 (Counterclockwise)

Preceding gene: 218904841

Following gene: 218904838

Centisome position: 67.07

GC content: 37.36

Gene sequence:

>954_bases
ATGGGAGAAGTGCAACGTGAAAAAGTTGCTGTCATCATTGGACCAACTGCTGTTGGGAAGACGAAGTTAAGTATTGATCT
TGCTAAAGCGTTGAACGGTGAAATTATAAGTGGAGATTCCATGCAGATTTATCGTACGATGGATATTGGAACTGCAAAGG
TGACGAAAGAAGAGGTGGATGGAATTCCACATTATATGGTCGATATAAAAAATCCGGAAGAATCATTTTCAGTTGCCGAG
TTTCAAGAACGTGTTCGTAAGCATATTCGAGAGATTACAGAGCGTGGTAAATTACCAATTATCGTTGGTGGAACTGGTCT
GTATATACAGTCTGTTTTATTCGATTATCAGTTTACGGATGATGCTGGTGATGCTATATACCGAGAACAAATGGAAAAGT
TAGCATTAGAACGTGGTGTGGAATATGTACATAAGAAATTACAAGAAGTAGACCCAGAAAGCGCTGAGCGTATTCATGCA
AATAATGTGCGTCGTGTCATTAGAGCGTTAGAAATTTTTCACACGTCGGGTGAAAAAATGAGTGATCAACTTGAAAAACA
AGAAAACGAGTTATTATACGATGTTTCCTTAATTGGCTTGACAATGGATCGTGAAATGCTATACGATCGCATTAACTTAC
GAGTTGATATAATGATGGACCAAGGTTTATTAGAAGAAGTAGAAGGGTTGTATAATAGAGGGATACGAGATTGTCAATCT
ATTCAAGCGATTGGGTATAAAGAGATATATGATTATTTTGAGGATCGTGTCTCTTTAGAAGAAGCGGTATCACAATTAAA
GACGAATTCACGTCGTTATGCAAAACGTCAATTAACGTGGTTCCGTAATAAGATGGATGTCACATGGTTTGATGTTACAG
ACGGTGAAAAAACGTCAGAAATTTTACGATACATRGAAGGAAAGCTACAACTAAAGTCGAATAATAGTAAGTAG

Upstream 100 bases:

>100_bases
CATGTACCGTAATGAAAGAACTTGCAAACATGCCGTTAAAATTGGTAAGATTGGATATAATCAATAGATGGAGTACATTA
CATAAAGAGAGTTGAATGAT

Downstream 100 bases:

>100_bases
AGAAAAAGAGGAGGATTCGACATGAAGCAATCAATCAATATTCAAGATCAGTTTTTAAATCAACTCCGTAAAGAGAATAC
GTTTGTTACGCTGTACTTAT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MGEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEVDGIPHYMVDIKNPEESFSVAE
FQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHA
NNVRRVIRALEIFHTSGEKMSDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQS
IQAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILRYXEGKLQLKSNNSK

Sequences:

>Translated_317_residues
MGEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEVDGIPHYMVDIKNPEESFSVAE
FQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHA
NNVRRVIRALEIFHTSGEKMSDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQS
IQAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILRYXEGKLQLKSNNSK
>Mature_316_residues
GEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEVDGIPHYMVDIKNPEESFSVAEF
QERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHAN
NVRRVIRALEIFHTSGEKMSDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQSI
QAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILRYXEGKLQLKSNNSK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=314, Percent_Identity=31.8471337579618, Blast_Score=155, Evalue=6e-38,
Organism=Escherichia coli, GI1790613, Length=294, Percent_Identity=38.7755102040816, Blast_Score=215, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI25144712, Length=307, Percent_Identity=32.5732899022801, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324848, Length=307, Percent_Identity=34.2019543973941, Blast_Score=179, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24649830, Length=341, Percent_Identity=31.3782991202346, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 36564; Mature: 36433

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEVD
CCCCCCCCEEEEECCCCCCCEEHHEEHHHHCCCCEECCCCEEEEEEECCCCHHCCHHHHC
GIPHYMVDIKNPEESFSVAEFQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTD
CCCEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCEECC
DAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHANNVRRVIRALEIFHTSGEKM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
SDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQS
HHHHHHHHHHEEEEEEEEEEEHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHCCHHHHHH
IQAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSE
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
ILRYXEGKLQLKSNNSK
HHHHHCCEEEEECCCCC
>Mature Secondary Structure 
GEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEVD
CCCCCCCEEEEECCCCCCCEEHHEEHHHHCCCCEECCCCEEEEEEECCCCHHCCHHHHC
GIPHYMVDIKNPEESFSVAEFQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTD
CCCEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCEECC
DAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHANNVRRVIRALEIFHTSGEKM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
SDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQS
HHHHHHHHHHEEEEEEEEEEEHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHCCHHHHHH
IQAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSE
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
ILRYXEGKLQLKSNNSK
HHHHHCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA