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The map label for this gene is nodB [H]

Identifier: 218904745

GI number: 218904745

Start: 3465710

End: 3466435

Strand: Reverse

Name: nodB [H]

Synonym: BCAH820_3629

Alternate gene names: 218904745

Gene position: 3466435-3465710 (Counterclockwise)

Preceding gene: 218904746

Following gene: 218904743

Centisome position: 65.37

GC content: 33.88

Gene sequence:

>726_bases
ATGTTAAGACAGAAAATTTTTCTATTCGTTTTTTCTTTATTATGCGCAGTACATATATTTCAAGTTGAAAAAGTGGAAGC
GAAAATGTTGCTCAGAAAAGAGCTAGAACCAACTGGCTATGTAACATGGGAAGTACCTAATAATGAAAAGATTATTGCCA
TTACATTTGATGATGGACCAGATCCAACTTATACACCACAAATTTTAAATTTATTACGTCAATATAAAGCAGAAGCGACA
TTTTTTATGATCGGATTTCGAGTTCAGCGTAATCCATATTTAGTGAAACAAGTGTTAAAAGAAGGACATGAAATTGGAAA
TCATACGATGAACCATTTGTATGCGAGTAATTCTTCAGATGAAAAATTAGAAAACGATATTTTAGATGGAAAGAAATTTT
TCAAAAAATGGGTGAAAGAACCTTTACTATTCCGTCCACCTGGCGGATATATTAACGATGCTGTATTTAAAACAGCGAAA
GAAGCTGGTTATCAAACTGTTCTATGGTCGTGGCATCAAGATCCACGTGATTGGGCAAATCCAGGGGTTGAATCCATTGT
AAATCATGTATTGAAAAATGCTAAAAGTGGCGATATTGTATTGTTACATGATGGAGGAAATAATCGTAGTCAAACAGTAG
CAGCGCTAGCAAAAATTTTACCTGAACTGAAAAAGCAGGGCTATCGATTTGTAACGGTTTCGGAATTATTACGTTATAAA
CATTAG

Upstream 100 bases:

>100_bases
TACGAAAATAATCTTCAAAATATTATGCAACATGTAGATCAATTAGAAAAGTAAATGAGGTTGTTGTGTATTCGACAACC
TTATTACTTTAATAAATGTT

Downstream 100 bases:

>100_bases
AAAAAATCCCAAAAGGTGAATGCTCCTTTTGGGATTTTTCGTTACTGGTTCTTTTTGCGTTTCTTATTATTTTGTCGTTC
TGCAGGAGTTAAGTTTTCAT

Product: putative polysaccharide deacetylase

Products: NA

Alternate protein names: Nodulation protein B [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MLRQKIFLFVFSLLCAVHIFQVEKVEAKMLLRKELEPTGYVTWEVPNNEKIIAITFDDGPDPTYTPQILNLLRQYKAEAT
FFMIGFRVQRNPYLVKQVLKEGHEIGNHTMNHLYASNSSDEKLENDILDGKKFFKKWVKEPLLFRPPGGYINDAVFKTAK
EAGYQTVLWSWHQDPRDWANPGVESIVNHVLKNAKSGDIVLLHDGGNNRSQTVAALAKILPELKKQGYRFVTVSELLRYK
H

Sequences:

>Translated_241_residues
MLRQKIFLFVFSLLCAVHIFQVEKVEAKMLLRKELEPTGYVTWEVPNNEKIIAITFDDGPDPTYTPQILNLLRQYKAEAT
FFMIGFRVQRNPYLVKQVLKEGHEIGNHTMNHLYASNSSDEKLENDILDGKKFFKKWVKEPLLFRPPGGYINDAVFKTAK
EAGYQTVLWSWHQDPRDWANPGVESIVNHVLKNAKSGDIVLLHDGGNNRSQTVAALAKILPELKKQGYRFVTVSELLRYK
H
>Mature_241_residues
MLRQKIFLFVFSLLCAVHIFQVEKVEAKMLLRKELEPTGYVTWEVPNNEKIIAITFDDGPDPTYTPQILNLLRQYKAEAT
FFMIGFRVQRNPYLVKQVLKEGHEIGNHTMNHLYASNSSDEKLENDILDGKKFFKKWVKEPLLFRPPGGYINDAVFKTAK
EAGYQTVLWSWHQDPRDWANPGVESIVNHVLKNAKSGDIVLLHDGGNNRSQTVAALAKILPELKKQGYRFVTVSELLRYK
H

Specific function: Is involved in generating a small heat-stable compound (Nod), an acylated oligomer of N-acetylglucosamine, that stimulates mitosis in various plant protoplasts [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323339, Length=167, Percent_Identity=34.1317365269461, Blast_Score=79, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6323338, Length=178, Percent_Identity=31.4606741573034, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 27800; Mature: 27800

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRQKIFLFVFSLLCAVHIFQVEKVEAKMLLRKELEPTGYVTWEVPNNEKIIAITFDDGP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCC
DPTYTPQILNLLRQYKAEATFFMIGFRVQRNPYLVKQVLKEGHEIGNHTMNHLYASNSSD
CCCCCHHHHHHHHHHHHCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EKLENDILDGKKFFKKWVKEPLLFRPPGGYINDAVFKTAKEAGYQTVLWSWHQDPRDWAN
HHHHHHHHCHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHCC
PGVESIVNHVLKNAKSGDIVLLHDGGNNRSQTVAALAKILPELKKQGYRFVTVSELLRYK
CCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCCC
H
C
>Mature Secondary Structure
MLRQKIFLFVFSLLCAVHIFQVEKVEAKMLLRKELEPTGYVTWEVPNNEKIIAITFDDGP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCC
DPTYTPQILNLLRQYKAEATFFMIGFRVQRNPYLVKQVLKEGHEIGNHTMNHLYASNSSD
CCCCCHHHHHHHHHHHHCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EKLENDILDGKKFFKKWVKEPLLFRPPGGYINDAVFKTAKEAGYQTVLWSWHQDPRDWAN
HHHHHHHHCHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHCC
PGVESIVNHVLKNAKSGDIVLLHDGGNNRSQTVAALAKILPELKKQGYRFVTVSELLRYK
CCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCCC
H
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7984092; 9163424 [H]