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The map label for this gene is hypF [H]

Identifier: 218904407

GI number: 218904407

Start: 3135731

End: 3138061

Strand: Reverse

Name: hypF [H]

Synonym: BCAH820_3291

Alternate gene names: 218904407

Gene position: 3138061-3135731 (Counterclockwise)

Preceding gene: 218904408

Following gene: 218904406

Centisome position: 59.18

GC content: 32.48

Gene sequence:

>2331_bases
ATGAAAAAAGTAATAAATATGTTTATAAAAAAGTATCAAGTTAAAGGCATTGTGCAAGGTGTTGGTTTTAGACCTTTCGT
ATATAGGATAGCAAAAAAGTATCATCTAAACGGATGGGTGTTAAATGATTCAAATGGAGTGCTTATAGAAGTAGAGGGGG
AACAAAATAATATTTCGCATTTTATTCATGATATTGAATTTAATTCCCCAGAATTGGCTTATGTGCAAGAGGTTAAATTG
ATAGAGGAACAAGAATCCAACAATCGTTATAGCTCTTTTGATATTTTGAGTAGCCAAAAACATAGTAGTAGGCAAACCTT
AATATCTCCTGATACTTATGTATGTAATGAATGTTTAGAAGAATTATTTGATCCAACGAATAGAAGGTTTCGTTATCCAT
TTATTAATTGTACAAATTGTGGACCTAGATATTCAATAATAAAAGATATTCCTTATGATAGACCGTATACTACAATGTCC
AAATTTCCTATGTGCAAAGAGTGTGATATAGAATACAAAGACATACTTGATCGGAGATTTCATGCACAACCAAATGCTTG
CTGGAAATGTGGACCACAAGTTCAATTGAAAACAACAGATGGTACAAATGTCTCCACTGATAATCCAATAGAAAAAACAA
TTGATTTGTTGAAAAAGGGGAAAATCCTAGCTATTAAAGGGTTAGGTGGATATCACTTAGTTGTAGATCCAATGAATCAT
GAAGCTGTAAAAGAATTAAGAAAAAGAAAAAAACGGGATGAAAAACCTTTTGCTTTAATGTCATCTCAATTAGATGATGT
GAAAAATTTTGCTGAAATAACAAAAAATGAAGAAACTTTATTAGTTTCTAAAGAACGACCAATTGTGTTATTGAACAAAA
AGGATAATGAGTATATTTCTTCAGATGTTGCACCTAGTAATAATACCTATGGGGTAATGTTAGCCTATACACCACTTCAT
CATCTCATTTTAAGAGGGAATTTTATTGCATTAATAGCTACGAGCGCCAATGTTTCTGATGAACCTATTGTATATAAAGA
TGCAGATGCAATTGAGCAGCTAAAAGGAATTGCAGATTATTATTTAGTACACAATAGGGAGATATTCACTAGAGTAGATG
ACTCTATTGTTAGGGGGATTGATTTAGATGATGAAATAACAAACCTACACATAATTCGTCGTGCTAGAGGTTATGTACCA
AACCCTATTGATTTTAAAGAAGGATCACCTAATGTACTTGCTTTAGGAGCAGAATTAAAGAATACGATTTGCCTAACTAA
AGACGAAAAAGCTTTTATTAGTCATCATATTGGTGATTTAAAAAACTATAAAATTTATGAATCGTTTAAAAATACTATAG
CTCACTTACAAAAAATCTTTGAAATTAGTCCGTCTGTAATTGCAAGTGATCTTCATCCTGATTTTTATAGTACAAAATAT
GCATTTGATCAAAATGTTTTACCAGTTGTACAGGTTCAGCATCATCATGCCCACATGGCTTCTTGTATGGTTGATAATCA
ATTAGATGGTCCTGTTTTAGGAGTTATTTTAGATGGTACGGGTTATGGTGATGATGGGAATATTTGGGGTGGAGAGTTTT
TAATCGGTGATTTTCTTAATTATGAAAGAGCGGCATATATCGATTACTTTCAGCTGCCAGGCGGTGATAAAGCTGTTAAA
GAACCGTATAGAATTGCTTTAGGAATATTGACGCAAATATATGGTGAACAAGTCAAAGAATTACCAATAAAGATAATTTC
AGAGCGAAATAGTTTTGAACTAGATGTACTTATAAAAATGATTCAAAAAGGAATTAATTCTCCTCTTACTTCAAGTATGG
GAAGGCTTTTTGATGCTGTTTCAGCACTATTGGGTGTCCGTGAAACAGTCCAATATGAAGGACAGGCTGCTATTGAGTTG
GAGCAAGCGATTGATATCAATAATTCTTCCAATCCTTTATTATCATATGAAATAGTTGCTTCGAATGGAATTAGAAAAAT
CGATATAAAACCTATGTTTAAAGAGTTGGTATCTTTGATACTTAAAGATAAGTATACCAAGGGTGAATTGAGCTATCGTT
TTCATTATACAATGGTTCAAATGATTACAGAAACCTGTAAACAAATTCGTCAAGAAACAGGGATTAATAAACTAGTTCTT
AGTGGGGGAGTATTTTTAAATAAGTTTTTACTTGTAGAAACCTATAAGTCACTTAAGGCTTGTAAATTCGAGGTTTACAT
GCACAAACAAGTACCTACCAATGATGGGGGAATCTCTCTTGGTCAAGCAGCAATAGCAGCTCATCGCTATAAAGATATGC
ATTCGGTATAA

Upstream 100 bases:

>100_bases
AAAGAAAGACAAGTACTCATGGAAGCAAAATCAGGTGAATTGAAACTCATTGAGTTATTATATGGGGCAGAATTACCTCG
ATTATGTTAGGAGACAATTT

Downstream 100 bases:

>100_bases
ATTTACAATAGCAATTATATAACTTTTCATTATTCTTTGTAAGTATAGTAAAAACGAAAAATTAGGAGGTGCTCTTTTAT
GGTAGTTAGTTCAAAAATGG

Product: hydrogenase maturation protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF2; [NiFe]-hydrogenase maturation factor hypF2; Hydrogenase maturation protein hypF2 [H]

Number of amino acids: Translated: 776; Mature: 776

Protein sequence:

>776_residues
MKKVINMFIKKYQVKGIVQGVGFRPFVYRIAKKYHLNGWVLNDSNGVLIEVEGEQNNISHFIHDIEFNSPELAYVQEVKL
IEEQESNNRYSSFDILSSQKHSSRQTLISPDTYVCNECLEELFDPTNRRFRYPFINCTNCGPRYSIIKDIPYDRPYTTMS
KFPMCKECDIEYKDILDRRFHAQPNACWKCGPQVQLKTTDGTNVSTDNPIEKTIDLLKKGKILAIKGLGGYHLVVDPMNH
EAVKELRKRKKRDEKPFALMSSQLDDVKNFAEITKNEETLLVSKERPIVLLNKKDNEYISSDVAPSNNTYGVMLAYTPLH
HLILRGNFIALIATSANVSDEPIVYKDADAIEQLKGIADYYLVHNREIFTRVDDSIVRGIDLDDEITNLHIIRRARGYVP
NPIDFKEGSPNVLALGAELKNTICLTKDEKAFISHHIGDLKNYKIYESFKNTIAHLQKIFEISPSVIASDLHPDFYSTKY
AFDQNVLPVVQVQHHHAHMASCMVDNQLDGPVLGVILDGTGYGDDGNIWGGEFLIGDFLNYERAAYIDYFQLPGGDKAVK
EPYRIALGILTQIYGEQVKELPIKIISERNSFELDVLIKMIQKGINSPLTSSMGRLFDAVSALLGVRETVQYEGQAAIEL
EQAIDINNSSNPLLSYEIVASNGIRKIDIKPMFKELVSLILKDKYTKGELSYRFHYTMVQMITETCKQIRQETGINKLVL
SGGVFLNKFLLVETYKSLKACKFEVYMHKQVPTNDGGISLGQAAIAAHRYKDMHSV

Sequences:

>Translated_776_residues
MKKVINMFIKKYQVKGIVQGVGFRPFVYRIAKKYHLNGWVLNDSNGVLIEVEGEQNNISHFIHDIEFNSPELAYVQEVKL
IEEQESNNRYSSFDILSSQKHSSRQTLISPDTYVCNECLEELFDPTNRRFRYPFINCTNCGPRYSIIKDIPYDRPYTTMS
KFPMCKECDIEYKDILDRRFHAQPNACWKCGPQVQLKTTDGTNVSTDNPIEKTIDLLKKGKILAIKGLGGYHLVVDPMNH
EAVKELRKRKKRDEKPFALMSSQLDDVKNFAEITKNEETLLVSKERPIVLLNKKDNEYISSDVAPSNNTYGVMLAYTPLH
HLILRGNFIALIATSANVSDEPIVYKDADAIEQLKGIADYYLVHNREIFTRVDDSIVRGIDLDDEITNLHIIRRARGYVP
NPIDFKEGSPNVLALGAELKNTICLTKDEKAFISHHIGDLKNYKIYESFKNTIAHLQKIFEISPSVIASDLHPDFYSTKY
AFDQNVLPVVQVQHHHAHMASCMVDNQLDGPVLGVILDGTGYGDDGNIWGGEFLIGDFLNYERAAYIDYFQLPGGDKAVK
EPYRIALGILTQIYGEQVKELPIKIISERNSFELDVLIKMIQKGINSPLTSSMGRLFDAVSALLGVRETVQYEGQAAIEL
EQAIDINNSSNPLLSYEIVASNGIRKIDIKPMFKELVSLILKDKYTKGELSYRFHYTMVQMITETCKQIRQETGINKLVL
SGGVFLNKFLLVETYKSLKACKFEVYMHKQVPTNDGGISLGQAAIAAHRYKDMHSV
>Mature_776_residues
MKKVINMFIKKYQVKGIVQGVGFRPFVYRIAKKYHLNGWVLNDSNGVLIEVEGEQNNISHFIHDIEFNSPELAYVQEVKL
IEEQESNNRYSSFDILSSQKHSSRQTLISPDTYVCNECLEELFDPTNRRFRYPFINCTNCGPRYSIIKDIPYDRPYTTMS
KFPMCKECDIEYKDILDRRFHAQPNACWKCGPQVQLKTTDGTNVSTDNPIEKTIDLLKKGKILAIKGLGGYHLVVDPMNH
EAVKELRKRKKRDEKPFALMSSQLDDVKNFAEITKNEETLLVSKERPIVLLNKKDNEYISSDVAPSNNTYGVMLAYTPLH
HLILRGNFIALIATSANVSDEPIVYKDADAIEQLKGIADYYLVHNREIFTRVDDSIVRGIDLDDEITNLHIIRRARGYVP
NPIDFKEGSPNVLALGAELKNTICLTKDEKAFISHHIGDLKNYKIYESFKNTIAHLQKIFEISPSVIASDLHPDFYSTKY
AFDQNVLPVVQVQHHHAHMASCMVDNQLDGPVLGVILDGTGYGDDGNIWGGEFLIGDFLNYERAAYIDYFQLPGGDKAVK
EPYRIALGILTQIYGEQVKELPIKIISERNSFELDVLIKMIQKGINSPLTSSMGRLFDAVSALLGVRETVQYEGQAAIEL
EQAIDINNSSNPLLSYEIVASNGIRKIDIKPMFKELVSLILKDKYTKGELSYRFHYTMVQMITETCKQIRQETGINKLVL
SGGVFLNKFLLVETYKSLKACKFEVYMHKQVPTNDGGISLGQAAIAAHRYKDMHSV

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=767, Percent_Identity=37.5488917861799, Blast_Score=476, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 88177; Mature: 88177

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVINMFIKKYQVKGIVQGVGFRPFVYRIAKKYHLNGWVLNDSNGVLIEVEGEQNNISH
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCHHH
FIHDIEFNSPELAYVQEVKLIEEQESNNRYSSFDILSSQKHSSRQTLISPDTYVCNECLE
HHEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEHHHCCCCCCCCCEEECCCHHHHHHHHH
ELFDPTNRRFRYPFINCTNCGPRYSIIKDIPYDRPYTTMSKFPMCKECDIEYKDILDRRF
HHHCCCCCEEECCEEEECCCCCCCHHHHCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHH
HAQPNACWKCGPQVQLKTTDGTNVSTDNPIEKTIDLLKKGKILAIKGLGGYHLVVDPMNH
CCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCEEEEECCCCH
EAVKELRKRKKRDEKPFALMSSQLDDVKNFAEITKNEETLLVSKERPIVLLNKKDNEYIS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCHHC
SDVAPSNNTYGVMLAYTPLHHLILRGNFIALIATSANVSDEPIVYKDADAIEQLKGIADY
CCCCCCCCCEEEEEEECHHHHHHHCCCEEEEEEECCCCCCCCEEEECHHHHHHHHHHHHH
YLVHNREIFTRVDDSIVRGIDLDDEITNLHIIRRARGYVPNPIDFKEGSPNVLALGAELK
HEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCHHC
NTICLTKDEKAFISHHIGDLKNYKIYESFKNTIAHLQKIFEISPSVIASDLHPDFYSTKY
CCEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHCCEE
AFDQNVLPVVQVQHHHAHMASCMVDNQLDGPVLGVILDGTGYGDDGNIWGGEFLIGDFLN
ECCCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHC
YERAAYIDYFQLPGGDKAVKEPYRIALGILTQIYGEQVKELPIKIISERNSFELDVLIKM
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCHHHHHHHH
IQKGINSPLTSSMGRLFDAVSALLGVRETVQYEGQAAIELEQAIDINNSSNPLLSYEIVA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCCCCEEEEEEEE
SNGIRKIDIKPMFKELVSLILKDKYTKGELSYRFHYTMVQMITETCKQIRQETGINKLVL
CCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEE
SGGVFLNKFLLVETYKSLKACKFEVYMHKQVPTNDGGISLGQAAIAAHRYKDMHSV
CCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKVINMFIKKYQVKGIVQGVGFRPFVYRIAKKYHLNGWVLNDSNGVLIEVEGEQNNISH
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCHHH
FIHDIEFNSPELAYVQEVKLIEEQESNNRYSSFDILSSQKHSSRQTLISPDTYVCNECLE
HHEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEHHHCCCCCCCCCEEECCCHHHHHHHHH
ELFDPTNRRFRYPFINCTNCGPRYSIIKDIPYDRPYTTMSKFPMCKECDIEYKDILDRRF
HHHCCCCCEEECCEEEECCCCCCCHHHHCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHH
HAQPNACWKCGPQVQLKTTDGTNVSTDNPIEKTIDLLKKGKILAIKGLGGYHLVVDPMNH
CCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCEEEEECCCCH
EAVKELRKRKKRDEKPFALMSSQLDDVKNFAEITKNEETLLVSKERPIVLLNKKDNEYIS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCHHC
SDVAPSNNTYGVMLAYTPLHHLILRGNFIALIATSANVSDEPIVYKDADAIEQLKGIADY
CCCCCCCCCEEEEEEECHHHHHHHCCCEEEEEEECCCCCCCCEEEECHHHHHHHHHHHHH
YLVHNREIFTRVDDSIVRGIDLDDEITNLHIIRRARGYVPNPIDFKEGSPNVLALGAELK
HEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCHHC
NTICLTKDEKAFISHHIGDLKNYKIYESFKNTIAHLQKIFEISPSVIASDLHPDFYSTKY
CCEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHCCEE
AFDQNVLPVVQVQHHHAHMASCMVDNQLDGPVLGVILDGTGYGDDGNIWGGEFLIGDFLN
ECCCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHC
YERAAYIDYFQLPGGDKAVKEPYRIALGILTQIYGEQVKELPIKIISERNSFELDVLIKM
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCHHHHHHHH
IQKGINSPLTSSMGRLFDAVSALLGVRETVQYEGQAAIELEQAIDINNSSNPLLSYEIVA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCCCCEEEEEEEE
SNGIRKIDIKPMFKELVSLILKDKYTKGELSYRFHYTMVQMITETCKQIRQETGINKLVL
CCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEE
SGGVFLNKFLLVETYKSLKACKFEVYMHKQVPTNDGGISLGQAAIAAHRYKDMHSV
CCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9799289; 12948488 [H]