Click here to switch to the map view.

The map label for this gene is yhcA [H]

Identifier: 218904346

GI number: 218904346

Start: 3070027

End: 3071505

Strand: Reverse

Name: yhcA [H]

Synonym: BCAH820_3230

Alternate gene names: 218904346

Gene position: 3071505-3070027 (Counterclockwise)

Preceding gene: 218904348

Following gene: 218904345

Centisome position: 57.92

GC content: 38.27

Gene sequence:

>1479_bases
ATGAGTGAAACGTTACATAAAAGAAAACCACCATATGTAATGCTTGTAATCCTTTTTATTGGGGCATTTGTTTCATTTCT
AAATAACTCGCTTTTAAATGTAGCCTTGCCATCAATAATGAAGGATTTAGACATTAAAGATTATTCAACGATCCAGTGGC
TTTCTACAGGATATATGCTTGTAAGTGGTATATTAATTCCCGCATCAGCATTTTTAATAACACGCTTCTCCAATAGGAGC
TTATTTATTACATCTATGATGATTTTTACTCTTGGTACGGCCTTAGCGGCTGTAGCACCGAACTTCGGTTTATTACTGAC
TGGCCGAATGGTTCAAGCAGCAGGTTCTTCAGTCATGGGGCCTTTGTTAATGAATATTATGCTAGTAAGCTTTCCGAGAG
AAAAAAGGGGAACAGCAATGGGGATTTTTGGACTAGTTATGATTACAGCCCCAGCTATTGGACCGACATTATCAGGTTAT
ATTGTAGAGTATTATGACTGGCGTTTGCTTTTTGAAATGATTTTACCGTTAGCAATTATTAGCTTACTGTTAGGGGTTTG
GAAATCGGAGAACGTCATGAGACAAAATAAAAATGCAAAACTTGATTATCTTTCATTACTATTATCTTCTGTCGGTTTTG
GCGGTTTGTTATATGGATTCAGTTCTGCAAGCTCCGATGGTTGGACGAATAAAGTTGTCGTAACAACCTTAATCATAGGC
GCTATTGCCCTTATTGCTTTCATTATCCGTCAATTAAAAATGAATGAACCGTTATTGGATTTACGTGTTTATAAGTATCC
AATGTTTGCACTTGCGTCTGTAATTGCGATTGTCAACGCAGTGGCCATGTTTTCGGGTATGATTTTAACACCAGCTTATG
TACAAAACGTTCGCGGTATTTCACCGCTGAGCTCTGGGCTGATGATGCTTCCAGGTGCCGTAATAATGGGGATTATGTCA
CCAATTACAGGGAAACTATTTGATAAATATGGTCCTCGTATTCTTGGTATAGTAGGACTTTCCATTACAGCCGTTTCAAC
TTATATGCTAGCAAACCTACAACTCGACTCTAGTCACACACATATTATTCTTATTTACACACTGCGGATGTTTGGGATGG
CGATGGTGATGATGCCGCTTATGACAAATGGCCTTAATCAATTGCCAACTCGCTTAAATCCGCACGGTACAGCTGTTAAT
AATACAGCTCAACAAGTATCCGGTTCAATTGGTACTGCTATTCTTGTTACCATTATGAATTCTGTAACAAAAACAAAAGC
TGAAAGTTTAATGGCTCATGTAGATCCAACAAACTTCACAGAAGTTACGAAGATAATGTTAACGCAGAAGGCTTTATTAT
CCGGAATTCAGCATTCATTCTATGTAGCACTTGGCATGAATGTCGTTGCGCTACTATTAGTTTTTTTCGTTAAACGTGTA
GATACAAGCCCGGAAGCTGTTGAGAGGTTAAATCGATAG

Upstream 100 bases:

>100_bases
AGTTTGGCAAAATACTGCAAGGACAACACGTATTCTTGTGCAAATATGCAAACGATTTAAACGAATCAATGTAATTTATA
ATGAAGGGGCAAATAAATAT

Downstream 100 bases:

>100_bases
AGCAAAATAAAAATCAAGATGATGATTTCTACTAAAAGGGAAAAAGAGCTGAAAGAAAAAGTGAAATAATTACACATAAT
GAAAAGGATCTGATATTCAT

Product: drug resistance transporter, EmrB/QacA family

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSIMKDLDIKDYSTIQWLSTGYMLVSGILIPASAFLITRFSNRS
LFITSMMIFTLGTALAAVAPNFGLLLTGRMVQAAGSSVMGPLLMNIMLVSFPREKRGTAMGIFGLVMITAPAIGPTLSGY
IVEYYDWRLLFEMILPLAIISLLLGVWKSENVMRQNKNAKLDYLSLLLSSVGFGGLLYGFSSASSDGWTNKVVVTTLIIG
AIALIAFIIRQLKMNEPLLDLRVYKYPMFALASVIAIVNAVAMFSGMILTPAYVQNVRGISPLSSGLMMLPGAVIMGIMS
PITGKLFDKYGPRILGIVGLSITAVSTYMLANLQLDSSHTHIILIYTLRMFGMAMVMMPLMTNGLNQLPTRLNPHGTAVN
NTAQQVSGSIGTAILVTIMNSVTKTKAESLMAHVDPTNFTEVTKIMLTQKALLSGIQHSFYVALGMNVVALLLVFFVKRV
DTSPEAVERLNR

Sequences:

>Translated_492_residues
MSETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSIMKDLDIKDYSTIQWLSTGYMLVSGILIPASAFLITRFSNRS
LFITSMMIFTLGTALAAVAPNFGLLLTGRMVQAAGSSVMGPLLMNIMLVSFPREKRGTAMGIFGLVMITAPAIGPTLSGY
IVEYYDWRLLFEMILPLAIISLLLGVWKSENVMRQNKNAKLDYLSLLLSSVGFGGLLYGFSSASSDGWTNKVVVTTLIIG
AIALIAFIIRQLKMNEPLLDLRVYKYPMFALASVIAIVNAVAMFSGMILTPAYVQNVRGISPLSSGLMMLPGAVIMGIMS
PITGKLFDKYGPRILGIVGLSITAVSTYMLANLQLDSSHTHIILIYTLRMFGMAMVMMPLMTNGLNQLPTRLNPHGTAVN
NTAQQVSGSIGTAILVTIMNSVTKTKAESLMAHVDPTNFTEVTKIMLTQKALLSGIQHSFYVALGMNVVALLLVFFVKRV
DTSPEAVERLNR
>Mature_491_residues
SETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSIMKDLDIKDYSTIQWLSTGYMLVSGILIPASAFLITRFSNRSL
FITSMMIFTLGTALAAVAPNFGLLLTGRMVQAAGSSVMGPLLMNIMLVSFPREKRGTAMGIFGLVMITAPAIGPTLSGYI
VEYYDWRLLFEMILPLAIISLLLGVWKSENVMRQNKNAKLDYLSLLLSSVGFGGLLYGFSSASSDGWTNKVVVTTLIIGA
IALIAFIIRQLKMNEPLLDLRVYKYPMFALASVIAIVNAVAMFSGMILTPAYVQNVRGISPLSSGLMMLPGAVIMGIMSP
ITGKLFDKYGPRILGIVGLSITAVSTYMLANLQLDSSHTHIILIYTLRMFGMAMVMMPLMTNGLNQLPTRLNPHGTAVNN
TAQQVSGSIGTAILVTIMNSVTKTKAESLMAHVDPTNFTEVTKIMLTQKALLSGIQHSFYVALGMNVVALLLVFFVKRVD
TSPEAVERLNR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=451, Percent_Identity=26.6075388026608, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789042, Length=487, Percent_Identity=23.8193018480493, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI87081983, Length=411, Percent_Identity=25.7907542579075, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1790195, Length=406, Percent_Identity=27.0935960591133, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788392, Length=405, Percent_Identity=26.1728395061728, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6322958, Length=522, Percent_Identity=21.6475095785441, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323735, Length=328, Percent_Identity=22.5609756097561, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53592; Mature: 53461

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.7 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSIMKDLDIKDYSTIQWLSTGYML
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VSGILIPASAFLITRFSNRSLFITSMMIFTLGTALAAVAPNFGLLLTGRMVQAAGSSVMG
HHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHCCCHHHH
PLLMNIMLVSFPREKRGTAMGIFGLVMITAPAIGPTLSGYIVEYYDWRLLFEMILPLAII
HHHHHHHHHHCCCHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLLLGVWKSENVMRQNKNAKLDYLSLLLSSVGFGGLLYGFSSASSDGWTNKVVVTTLIIG
HHHHHHCCCCHHHHCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHH
AIALIAFIIRQLKMNEPLLDLRVYKYPMFALASVIAIVNAVAMFSGMILTPAYVQNVRGI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHCCCC
SPLSSGLMMLPGAVIMGIMSPITGKLFDKYGPRILGIVGLSITAVSTYMLANLQLDSSHT
CHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHEEEECCCCC
HIILIYTLRMFGMAMVMMPLMTNGLNQLPTRLNPHGTAVNNTAQQVSGSIGTAILVTIMN
EEEHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHH
SVTKTKAESLMAHVDPTNFTEVTKIMLTQKALLSGIQHSFYVALGMNVVALLLVFFVKRV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DTSPEAVERLNR
CCCHHHHHHHCC
>Mature Secondary Structure 
SETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSIMKDLDIKDYSTIQWLSTGYML
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VSGILIPASAFLITRFSNRSLFITSMMIFTLGTALAAVAPNFGLLLTGRMVQAAGSSVMG
HHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHCCCHHHH
PLLMNIMLVSFPREKRGTAMGIFGLVMITAPAIGPTLSGYIVEYYDWRLLFEMILPLAII
HHHHHHHHHHCCCHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLLLGVWKSENVMRQNKNAKLDYLSLLLSSVGFGGLLYGFSSASSDGWTNKVVVTTLIIG
HHHHHHCCCCHHHHCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHH
AIALIAFIIRQLKMNEPLLDLRVYKYPMFALASVIAIVNAVAMFSGMILTPAYVQNVRGI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHCCCC
SPLSSGLMMLPGAVIMGIMSPITGKLFDKYGPRILGIVGLSITAVSTYMLANLQLDSSHT
CHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHEEEECCCCC
HIILIYTLRMFGMAMVMMPLMTNGLNQLPTRLNPHGTAVNNTAQQVSGSIGTAILVTIMN
EEEHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHH
SVTKTKAESLMAHVDPTNFTEVTKIMLTQKALLSGIQHSFYVALGMNVVALLLVFFVKRV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DTSPEAVERLNR
CCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]