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The map label for this gene is ywbC [H]

Identifier: 218904333

GI number: 218904333

Start: 3056594

End: 3056986

Strand: Direct

Name: ywbC [H]

Synonym: BCAH820_3217

Alternate gene names: 218904333

Gene position: 3056594-3056986 (Clockwise)

Preceding gene: 218904330

Following gene: 218904334

Centisome position: 57.64

GC content: 38.68

Gene sequence:

>393_bases
ATGCCTGTTAGAAGAATTGAACACGTTGGACTTATGGTTGCAAACTTAGAAACATCTATTACTTTTTATGAAAAAGTAGT
TGGCTTACAGCTCATTAAACGTATGGGGCACCCGAATCCAGACTTAAAACTCGCTTTTTTAGGTGTGGAAGAATCAAAAG
AGACGATACTCGAATTAATTGAAGGCTACAACTCTTCCCTTCCGGCAGAAGGAAAAGTACATCACATTTGCTTTAAAGTG
GATTCATTAGAGGATGAAATTGAAAGACTAAAGAAACATGCAGTAACCTTCTTACTAGGAGAAGAAATCGAAACATTACC
GGATGGAACACGTTACATATTCTTCGCGGGTCCTGATGGAGAGTGGATTGAGTTTTTTGAGACGGAGAGATGA

Upstream 100 bases:

>100_bases
ATACCATATAACACGCTAAAATTTGTAAGGTTTTTATCTTGCTTATTTTCTTAATTTTCAATATTATATTGTATATAATT
TTCAGAAAGAAGGAATCTAC

Downstream 100 bases:

>100_bases
AGTAAACCTTTATGTTGCGGGATAGAAAAGGAGCCGGAATTCCGGCCCTTGTTTTTAACCAGTAACAATGAAACATACCT
CCAACCTGAGAATTCACCAA

Product: glyoxylase family protein

Products: glutathione; methylglyoxal

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MPVRRIEHVGLMVANLETSITFYEKVVGLQLIKRMGHPNPDLKLAFLGVEESKETILELIEGYNSSLPAEGKVHHICFKV
DSLEDEIERLKKHAVTFLLGEEIETLPDGTRYIFFAGPDGEWIEFFETER

Sequences:

>Translated_130_residues
MPVRRIEHVGLMVANLETSITFYEKVVGLQLIKRMGHPNPDLKLAFLGVEESKETILELIEGYNSSLPAEGKVHHICFKV
DSLEDEIERLKKHAVTFLLGEEIETLPDGTRYIFFAGPDGEWIEFFETER
>Mature_129_residues
PVRRIEHVGLMVANLETSITFYEKVVGLQLIKRMGHPNPDLKLAFLGVEESKETILELIEGYNSSLPAEGKVHHICFKVD
SLEDEIERLKKHAVTFLLGEEIETLPDGTRYIFFAGPDGEWIEFFETER

Specific function: Catalyzes The Conversion Of Hemimercaptal, Formed From Methylglyoxal And Glutathione, To S-Lactoylglutathione. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: 4.4.1.5

Molecular weight: Translated: 14888; Mature: 14757

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVRRIEHVGLMVANLETSITFYEKVVGLQLIKRMGHPNPDLKLAFLGVEESKETILELI
CCCCHHHHHCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHH
EGYNSSLPAEGKVHHICFKVDSLEDEIERLKKHAVTFLLGEEIETLPDGTRYIFFAGPDG
HCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEECCCC
EWIEFFETER
CEEHEEECCC
>Mature Secondary Structure 
PVRRIEHVGLMVANLETSITFYEKVVGLQLIKRMGHPNPDLKLAFLGVEESKETILELI
CCCHHHHHCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHH
EGYNSSLPAEGKVHHICFKVDSLEDEIERLKKHAVTFLLGEEIETLPDGTRYIFFAGPDG
HCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEECCCC
EWIEFFETER
CEEHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 20280 [C]

Specific activity: NA

Km value (mM): 0.0272 {methylglyoxal/glutathione} [C]

Substrates: (R)-S-lactoylglutathione

Specific reaction: (R)-S-lactoylglutathione = glutathione + methylglyoxal

General reaction: addition of RSH [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]