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The map label for this gene is brnQ5 [H]

Identifier: 218904269

GI number: 218904269

Start: 2989536

End: 2990888

Strand: Reverse

Name: brnQ5 [H]

Synonym: BCAH820_3153

Alternate gene names: 218904269

Gene position: 2990888-2989536 (Counterclockwise)

Preceding gene: 218904270

Following gene: 218904268

Centisome position: 56.4

GC content: 33.26

Gene sequence:

>1353_bases
ATGTCAAATAAAGTTCCTACTTCTTTTATCATTATTATAGGATTGATGCTATTTGCATTGTTTTTTGGAGCAGGAAATTT
AATTTTTCCACCTATGCTTGGTCAAATGGCTGGAAATAATGTATGGATAGCCAATGCAGGCTTTTTAGTTACTGGAGTTG
GTTTACCATTGTTAGCAATAACAGCATTTGTTTTTTCAGGTGAAAGTGATTTGCAATCTTTAGCTAGTCGTGTACATCCG
GTGTTTGGTATTGTATTTGCGATCGTTCTCTATCTAGCGATTGGTCCCTTTTATGCCATCCCTAGATCTGGAACTGTATC
ATTTGAAATTGGAATTAAACCGTTTTTATCAAATGATGCAGGTCCTGCTGCATTAACCGTATTTACAATCTTATTTTTCA
CAGTTACATGCTTATTATCCCTTAATCCTACAAAACTTGTTGATATAGTTGGAAAAATTTTGACTCCTATCAAATTGACT
GTTATTGGGATGCTCGTAGTTGTTACTTTTATTCACCCAATTGGAAGAATTCAGGCACCTAGTGAAGTGTATGCATCAGA
TGCATTTTTTAAAGGTTTTCAAGAAGGGTATTTAACATTAGATGCTCTTGGAGCTTTTGTTTTCGGAATTATTATTGTAA
ATGCAATTAGAGAAAAAGGGGCTACTACTAAAAAGCAGCTTATGGTTGTTTGTGTAAAAGCAACGACTATTGCTGCTACA
CTCTTAGCTCTTATCTATACTGCCCTTTCCTATATGGGCGCTTCTAGTGTAGAAAAACTAGGTCATTTAAATAATGGGGC
TGAAGTTTTAGCAAAAGTTTCGGGCTATTACTTTGGTTCGTATGGTGCAATCTTCTTAGGTGTAATGATTATAGTAGCAT
GTTTAATCACAAGCGTAGGACTTATCACTGCTTGTTCTTCTTTTTTTCATAAATTATTTCCAAAGATTTCTTACAAAAAA
CTTGCTATTATTTTGTCTGTTTTCAGTACACTAGTTGCGAATATAGGGTTAACACAATTAATTAAGATTTCAATACCTGT
ATTAATAACTATGTATCCAATTGCTATTACCTTAATATTTCTAACATTTTTACACTCTGTATTCAATGGTAGATTTGAAG
TATATCAAGGGAGTTTGATATTGACATTTATTTTTAGTTCGTTAGATGGATTAAAAGCTGCTGGAATAGAAAGCGAAATA
ATCCATAATTTTTTTGAGCACTTTCTTCCTTTATATAGTGTAGGTTTAGGGTGGGGATTTCCGGCTATTATAGGCGGTGT
ATGTGGGTATGTTGTTAGTGTATTACGGAAGAAAAATACCTTAAGTTCTAGTGAAACTCAATTAAATAAATAA

Upstream 100 bases:

>100_bases
TTGCCTTTCACAATGGTGCTCTAGGCTGGAATAAACTAGATTAATATATATTATTTAAAAAATAAAGAGCGTATACAAAT
GTATAATCGGAGGAGTTGTT

Downstream 100 bases:

>100_bases
ATTATTTTTGTTTATCATACCATTATCGGGCATACAAAACTAATTATGTCTTTGAAATGAAAAAATCTTTTATTTATATC
TTTGGAGGTAAATTGAATTA

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSNKVPTSFIIIIGLMLFALFFGAGNLIFPPMLGQMAGNNVWIANAGFLVTGVGLPLLAITAFVFSGESDLQSLASRVHP
VFGIVFAIVLYLAIGPFYAIPRSGTVSFEIGIKPFLSNDAGPAALTVFTILFFTVTCLLSLNPTKLVDIVGKILTPIKLT
VIGMLVVVTFIHPIGRIQAPSEVYASDAFFKGFQEGYLTLDALGAFVFGIIIVNAIREKGATTKKQLMVVCVKATTIAAT
LLALIYTALSYMGASSVEKLGHLNNGAEVLAKVSGYYFGSYGAIFLGVMIIVACLITSVGLITACSSFFHKLFPKISYKK
LAIILSVFSTLVANIGLTQLIKISIPVLITMYPIAITLIFLTFLHSVFNGRFEVYQGSLILTFIFSSLDGLKAAGIESEI
IHNFFEHFLPLYSVGLGWGFPAIIGGVCGYVVSVLRKKNTLSSSETQLNK

Sequences:

>Translated_450_residues
MSNKVPTSFIIIIGLMLFALFFGAGNLIFPPMLGQMAGNNVWIANAGFLVTGVGLPLLAITAFVFSGESDLQSLASRVHP
VFGIVFAIVLYLAIGPFYAIPRSGTVSFEIGIKPFLSNDAGPAALTVFTILFFTVTCLLSLNPTKLVDIVGKILTPIKLT
VIGMLVVVTFIHPIGRIQAPSEVYASDAFFKGFQEGYLTLDALGAFVFGIIIVNAIREKGATTKKQLMVVCVKATTIAAT
LLALIYTALSYMGASSVEKLGHLNNGAEVLAKVSGYYFGSYGAIFLGVMIIVACLITSVGLITACSSFFHKLFPKISYKK
LAIILSVFSTLVANIGLTQLIKISIPVLITMYPIAITLIFLTFLHSVFNGRFEVYQGSLILTFIFSSLDGLKAAGIESEI
IHNFFEHFLPLYSVGLGWGFPAIIGGVCGYVVSVLRKKNTLSSSETQLNK
>Mature_449_residues
SNKVPTSFIIIIGLMLFALFFGAGNLIFPPMLGQMAGNNVWIANAGFLVTGVGLPLLAITAFVFSGESDLQSLASRVHPV
FGIVFAIVLYLAIGPFYAIPRSGTVSFEIGIKPFLSNDAGPAALTVFTILFFTVTCLLSLNPTKLVDIVGKILTPIKLTV
IGMLVVVTFIHPIGRIQAPSEVYASDAFFKGFQEGYLTLDALGAFVFGIIIVNAIREKGATTKKQLMVVCVKATTIAATL
LALIYTALSYMGASSVEKLGHLNNGAEVLAKVSGYYFGSYGAIFLGVMIIVACLITSVGLITACSSFFHKLFPKISYKKL
AIILSVFSTLVANIGLTQLIKISIPVLITMYPIAITLIFLTFLHSVFNGRFEVYQGSLILTFIFSSLDGLKAAGIESEII
HNFFEHFLPLYSVGLGWGFPAIIGGVCGYVVSVLRKKNTLSSSETQLNK

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=445, Percent_Identity=40.4494382022472, Blast_Score=318, Evalue=4e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 48284; Mature: 48153

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKVPTSFIIIIGLMLFALFFGAGNLIFPPMLGQMAGNNVWIANAGFLVTGVGLPLLAI
CCCCCCHHHHHHHHHHHHHHHHCCCCEECCCHHHHHCCCCEEEEECCHHHHHCCHHHHHH
TAFVFSGESDLQSLASRVHPVFGIVFAIVLYLAIGPFYAIPRSGTVSFEIGIKPFLSNDA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEECCCHHCCCCC
GPAALTVFTILFFTVTCLLSLNPTKLVDIVGKILTPIKLTVIGMLVVVTFIHPIGRIQAP
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SEVYASDAFFKGFQEGYLTLDALGAFVFGIIIVNAIREKGATTKKQLMVVCVKATTIAAT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LLALIYTALSYMGASSVEKLGHLNNGAEVLAKVSGYYFGSYGAIFLGVMIIVACLITSVG
HHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LITACSSFFHKLFPKISYKKLAIILSVFSTLVANIGLTQLIKISIPVLITMYPIAITLIF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LTFLHSVFNGRFEVYQGSLILTFIFSSLDGLKAAGIESEIIHNFFEHFLPLYSVGLGWGF
HHHHHHHHCCCEEEEECCHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCH
PAIIGGVCGYVVSVLRKKNTLSSSETQLNK
HHHHHHHHHHHHHHHHHHCCCCCCHHHCCC
>Mature Secondary Structure 
SNKVPTSFIIIIGLMLFALFFGAGNLIFPPMLGQMAGNNVWIANAGFLVTGVGLPLLAI
CCCCCHHHHHHHHHHHHHHHHCCCCEECCCHHHHHCCCCEEEEECCHHHHHCCHHHHHH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HHHHHHHHHHHHHHHHHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]