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The map label for this gene is ansA2 [H]

Identifier: 218904252

GI number: 218904252

Start: 2967884

End: 2968888

Strand: Reverse

Name: ansA2 [H]

Synonym: BCAH820_3136

Alternate gene names: 218904252

Gene position: 2968888-2967884 (Counterclockwise)

Preceding gene: 218904256

Following gene: 218904251

Centisome position: 55.99

GC content: 35.82

Gene sequence:

>1005_bases
ATGAAGAAAATTTTATTACTTGCAACTGGTGGAACAATTGCATCTGTGGAGGGGAATGAGGGGCTTGTTCCAGGATTGTC
CGCTGAGGAATTATTGAATTATTTTTCGAGGTCGTCTCGAAACTTAGAAATAGATTGCAAAATCTTACTGAATATTGATA
GTACCAATATGCAACCTGAGCACTGGAAAGAAATAGCTAATGCTGTTTTTAATCACTATGATGACTATGACGGGTTTGTG
ATTACACATGGAACGGATACGTTAGCTTATACATCTTCTGCGTTATCTTATATGCTGCAAGGTTTAAGAAAACCAGTTGT
TTTAACAGGCTCACAGGTGCCTATTAGTTTTAAAAAGACAGACGCAAAGAAAAATGTAGCTGATGCTCTTAGATTTGCCT
GTGAGGATGTTGGCGGTGTGTTTATTGTTTTTGATAGTAGGGTGATTATTGGGACGAGAGCAGTTAAAATGCGAACAAAA
AGTTATGATGCATTTGAAAGTGTGAATTATCCATATGTTGCTGAGGTAAATGAAGATGAAGTGAAATATCATTGGAAGCC
AACGTCCTCCCATAATGAACTTTCAATAAATACTAATTTATGTACGGATGTTTTTCTTATGAAATTGTATCCAGGTACAA
AGCCAGAGATATTTGATTGTTTAAAAGATTTGTATAAAGGGGTTATTATTGAAAGTTTTGGTAACGGTGGATTACCTTTT
GAAGGAAGGAACTTGCTTTCGAAAATTCAAGAGTTGACTGAAATGGGGATTGCTGTAGTGATTACTACTCAATGCCTGGA
AGAAGGAGAAGACATACTTCTCTATGAAGTAGGACGGAAAGTGGCGCAACATCAAGTAATATTGTCTGGAGATATGAATA
CGGAAGCCATCATAGCAAAATTGATGTGGACGCTTGGAAAAACTAATAAATTAGAAGAGATTAAAAAAATTATTGAAGAG
CCTTTAGCCTACGATTTAACAATAAAGTCTGATAAGGATTGGTAA

Upstream 100 bases:

>100_bases
TACAAAATCTTGATTAATTCAAATTAAGTTCTATAATGATAAATATAAATACGCATTACGCGAACGATATATCGTTGTTT
ATTTTATGGAGGGGTTGTGT

Downstream 100 bases:

>100_bases
TGACTTCATATGATTTATTTAGGTTTTACATTTAATATGGGAGAGAAATTAAAATGTCAATGACAAACAAGAACATGCGA
GTGGAGAAGGATTTTTTGGG

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase 1; L-asparagine amidohydrolase 1 [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSRSSRNLEIDCKILLNIDSTNMQPEHWKEIANAVFNHYDDYDGFV
ITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKTDAKKNVADALRFACEDVGGVFIVFDSRVIIGTRAVKMRTK
SYDAFESVNYPYVAEVNEDEVKYHWKPTSSHNELSINTNLCTDVFLMKLYPGTKPEIFDCLKDLYKGVIIESFGNGGLPF
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAKLMWTLGKTNKLEEIKKIIEE
PLAYDLTIKSDKDW

Sequences:

>Translated_334_residues
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSRSSRNLEIDCKILLNIDSTNMQPEHWKEIANAVFNHYDDYDGFV
ITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKTDAKKNVADALRFACEDVGGVFIVFDSRVIIGTRAVKMRTK
SYDAFESVNYPYVAEVNEDEVKYHWKPTSSHNELSINTNLCTDVFLMKLYPGTKPEIFDCLKDLYKGVIIESFGNGGLPF
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAKLMWTLGKTNKLEEIKKIIEE
PLAYDLTIKSDKDW
>Mature_334_residues
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSRSSRNLEIDCKILLNIDSTNMQPEHWKEIANAVFNHYDDYDGFV
ITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKTDAKKNVADALRFACEDVGGVFIVFDSRVIIGTRAVKMRTK
SYDAFESVNYPYVAEVNEDEVKYHWKPTSSHNELSINTNLCTDVFLMKLYPGTKPEIFDCLKDLYKGVIIESFGNGGLPF
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAKLMWTLGKTNKLEEIKKIIEE
PLAYDLTIKSDKDW

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=360, Percent_Identity=31.6666666666667, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1788065, Length=335, Percent_Identity=34.0298507462687, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1789327, Length=303, Percent_Identity=24.7524752475248, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17558278, Length=362, Percent_Identity=30.6629834254144, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI193207372, Length=362, Percent_Identity=30.6629834254144, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17558280, Length=362, Percent_Identity=30.6629834254144, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI193207374, Length=362, Percent_Identity=30.6629834254144, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6320527, Length=331, Percent_Identity=25.0755287009063, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323189, Length=279, Percent_Identity=26.5232974910394, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323187, Length=279, Percent_Identity=26.5232974910394, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323186, Length=279, Percent_Identity=26.5232974910394, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323184, Length=279, Percent_Identity=26.5232974910394, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24639609, Length=282, Percent_Identity=35.1063829787234, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24645530, Length=266, Percent_Identity=36.4661654135338, Blast_Score=164, Evalue=9e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37370; Mature: 37370

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSRSSRNLEIDCKILLNIDSTNMQPE
CCEEEEEEECCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHH
HWKEIANAVFNHYDDYDGFVITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKT
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECC
DAKKNVADALRFACEDVGGVFIVFDSRVIIGTRAVKMRTKSYDAFESVNYPYVAEVNEDE
CCHHHHHHHHHHHHHHCCCEEEEECCEEEEEEEEEEEECCCCHHHHCCCCCEEEECCCCC
VKYHWKPTSSHNELSINTNLCTDVFLMKLYPGTKPEIFDCLKDLYKGVIIESFGNGGLPF
EEEEECCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHCEEEEECCCCCCCC
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAK
CCHHHHHHHHHHHHCCEEEEEEEHHHHCCCCEEEEHHHHHHHHCEEEEECCCCHHHHHHH
LMWTLGKTNKLEEIKKIIEEPLAYDLTIKSDKDW
HHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCC
>Mature Secondary Structure
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSRSSRNLEIDCKILLNIDSTNMQPE
CCEEEEEEECCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHH
HWKEIANAVFNHYDDYDGFVITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKT
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECC
DAKKNVADALRFACEDVGGVFIVFDSRVIIGTRAVKMRTKSYDAFESVNYPYVAEVNEDE
CCHHHHHHHHHHHHHHCCCEEEEECCEEEEEEEEEEEECCCCHHHHCCCCCEEEECCCCC
VKYHWKPTSSHNELSINTNLCTDVFLMKLYPGTKPEIFDCLKDLYKGVIIESFGNGGLPF
EEEEECCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHCEEEEECCCCCCCC
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAK
CCHHHHHHHHHHHHCCEEEEEEEHHHHCCCCEEEEHHHHHHHHCEEEEECCCCHHHHHHH
LMWTLGKTNKLEEIKKIIEEPLAYDLTIKSDKDW
HHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377; 8478318 [H]