Click here to switch to the map view.

The map label for this gene is ppsA [H]

Identifier: 218904235

GI number: 218904235

Start: 2951639

End: 2954248

Strand: Reverse

Name: ppsA [H]

Synonym: BCAH820_3119

Alternate gene names: 218904235

Gene position: 2954248-2951639 (Counterclockwise)

Preceding gene: 218904236

Following gene: 218904233

Centisome position: 55.71

GC content: 37.78

Gene sequence:

>2610_bases
ATGAGTTCTTTCGTTCTCGATTTTCAGGAAATAGAAAAAACGCAGCTTTCACTTGTTGGTGGAAAAGGTTTGAATTTAGG
GGCGTTATCTAACATGCAAGAGATACAAGTGCCAGAAGGTTTTTGTGTTACAACGGCAGGATATGAAAAGGCCATCGAGC
AAAATGAAGGGCTTCAAACTTTGTTGCAGCAACTAACAAAGTTGAAAAAGGAAGACCGAGCTCAAATTGGTGAAATGAGT
AAGGAAATTAGAGAAGTCATTATGGCAGTACAAATCCCTTCTGATGTAGTGGAAGCGGTAGCTCATTATCTCTCACGTTT
TGGAAATGAACATGCGTATGCCGTGCGTTCTAGTGCTACGGCTGAAGATTTACCGTATGCCTCGTTTGCAGGTCAACAAG
ATACGTATTTAAATATTATCGGAAAAGAAGCGATTTTACAGCATGTAAGAAAGTGCTGGGCTTCTTTATTTACAGAGCGA
GCAGTCATGTACCGTATGCAAAATGGTTTTGAACATAATCAAGTTTCTATATGTATTGTTGTTCAAAAAATGGTTTTCCC
GCAGGCTTCAGGAATTTTATTTACCGCTGATCCGATTACTTCTAACCGGAAGGTGTTATCAATCGATGCCAGTTTTGGAC
TTGGCGAGGCATTAGTATCGGGACTCGTCTCTGCCGATAATTATAAAGTAAAAGAAGGCGAAATCATCGATAAGATGATT
GCAACTAAAAAATTAGCTATTTATGCTTTAAAAGAGGGCGGAACTGAGACGAAACAGAATGATCCGGTTCAGCAAAAAAT
TCAAACACTATCTGACCAACAAATATTACAACTCGCACAGATTGGAAGACAGATTGAAGCTTATTTTGGCTGTCCGCAAG
ATATTGAATGGTGTTTAGTTGATAATGCATTTTATATTGTCCAAAGTCGTCCGATAACGACTTTATATCCAATTCCGGAA
GAAAATGATGAGGGAAATCATGTGTATATATCAGTTGGTCATCAACAAATGATGACCGATGCGATGAAACCACTAGGGTT
ATCTTTTTTCTTGTTAACGACTAATGCTCCTATGCGTGAAGCGGGAGGCAGGCTGTTTGTAGATGCTACGCAACAATTAG
CTTTACCTGCTAGCAGAGATTATTTAATAAACACTTTAGGGAAATCGGATCCCCTTGTAAGAGATGCATTAACGACTATA
ATCGAGCGAGATGATTTTATTACATTATTACCGGATGAAGAAAAAAAAGAAAAGAGTGCTAGTAAAAGTAAGCTACCAGT
AAGTTCGCAACTAGAAATCGAAAATGATCCAGCAATCGTTACGGAATTAATAAGGAATAGCGAAGCATCATTTGAAGAGT
TAAAACAAAATATGCAACTGAAATCAGGAGTGGATGTATTTGATTTTATTTTGGAAGACATCCAGCAATTAAAGAAAGTA
TTATTTAATCCGCAAAGTATAGCTGTCATTATGGTAGGTATGAATGCTTCAACATGGATTAATGAAAAAATGGAGAAGTG
GCTAGGTGAAAAGAATGCAGCAGACACGCTTTCTCAATCGGTACAAAATAATATTACATCAGAAATGGGTTTAGCATTAA
TGGATGTTGCTGATGCAATTCGTCCGTATCCAGAAGTTATTACGTATTTACAACATGTAGAAGATGATAGCTTTTTAGAT
GAACTTGTTCAGTATAAAGGCGGAGAAAAAGCTCGAGATGCAATCGTTGCTTTTCTAAATAAATACGGAATGAGATGTAG
CGGCGAAATCGACATTACGAAAACGCGCTGGAGTGAGAAGCCAACAACCATTATCCCAATGATATTAAATAATATAAGAG
ACTTTGAAGCGGGTGCTAGTAAGCAGAAGTTTGAAAAAGGACTGCAGGAAGCTTTGAATAAAGAAGAAGAGTTAGTAGAT
CGATTGCAGCACTTGCCGGATGGAAAACAAAAGATAGAAGAAACGAAGCGAATGATTCGTAATATTCGTAATTTTATTGG
CTATCGTGAATATCCGAAATACGGCATGATTAATCGTTACTTCATATATAAGCAGGCGTTACTGAAAGAAGCTGAGCAAC
TTGTACAAAGCGGTGTCATTCATGGAGTTGATGATATATACTACTTAACTTTTGAAGAGCTTCACGAAGTTGTTCGCACG
AAGAAACTAAATTATGATCTTATTCATAAACAAAAAAATGATTATAAATTATACGAAAAACTAACGCCTCCTCGTATAAT
GACGTCTGATGGAGAAATCATTACAGGTAAATATAAACGAGAAAACCTCCCAGTTGACGCGATTGCAGGTCTGCCTGTTT
CTTCTGGATTAGTTGAGGGGAGAGCTCGCGTTATTTTAAACATGGAAGATGCGAATTTAGAAGAGGGAGATATATTAGTT
ACAGCTTTTACTGACCCTGGCTGGACACCATTATTTGTATCTATAAAAGGATTAGTTACTGAAGTCGGCGGACTTATGAC
GCACGGAGCAGTGATTGCACGTGAATATGGATTACCAGCAGTTGTTGGGGTAGAGAATGCTACGAAATTAATAAAAGATG
GGCAACGAATACGAGTACATGGAACGGAAGGGTATATTGAAGTGTTGTAA

Upstream 100 bases:

>100_bases
AACTTAATTATACCACGTAAATAAAGTTCGAAATAGACTGTTTCTTCTCATGTACTACTGCTACAATCAAGAACACATGC
ATAGTAAGGAGAGATGTTAC

Downstream 100 bases:

>100_bases
TGAAGTAAAAGATCATCCGCTATAAATAGTGGATGATCTTTTTATTTTGCAATAACTCACTTCTTTAAGCCAGCTGCATA
AAAAGGTCCATGAACTGCGA

Product: phosphoenolpyruvate synthase

Products: AMP; phosphoenolpyruvate; phosphate

Alternate protein names: Putative PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 869; Mature: 868

Protein sequence:

>869_residues
MSSFVLDFQEIEKTQLSLVGGKGLNLGALSNMQEIQVPEGFCVTTAGYEKAIEQNEGLQTLLQQLTKLKKEDRAQIGEMS
KEIREVIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSATAEDLPYASFAGQQDTYLNIIGKEAILQHVRKCWASLFTER
AVMYRMQNGFEHNQVSICIVVQKMVFPQASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSADNYKVKEGEIIDKMI
ATKKLAIYALKEGGTETKQNDPVQQKIQTLSDQQILQLAQIGRQIEAYFGCPQDIEWCLVDNAFYIVQSRPITTLYPIPE
ENDEGNHVYISVGHQQMMTDAMKPLGLSFFLLTTNAPMREAGGRLFVDATQQLALPASRDYLINTLGKSDPLVRDALTTI
IERDDFITLLPDEEKKEKSASKSKLPVSSQLEIENDPAIVTELIRNSEASFEELKQNMQLKSGVDVFDFILEDIQQLKKV
LFNPQSIAVIMVGMNASTWINEKMEKWLGEKNAADTLSQSVQNNITSEMGLALMDVADAIRPYPEVITYLQHVEDDSFLD
ELVQYKGGEKARDAIVAFLNKYGMRCSGEIDITKTRWSEKPTTIIPMILNNIRDFEAGASKQKFEKGLQEALNKEEELVD
RLQHLPDGKQKIEETKRMIRNIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVIHGVDDIYYLTFEELHEVVRT
KKLNYDLIHKQKNDYKLYEKLTPPRIMTSDGEIITGKYKRENLPVDAIAGLPVSSGLVEGRARVILNMEDANLEEGDILV
TAFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKLIKDGQRIRVHGTEGYIEVL

Sequences:

>Translated_869_residues
MSSFVLDFQEIEKTQLSLVGGKGLNLGALSNMQEIQVPEGFCVTTAGYEKAIEQNEGLQTLLQQLTKLKKEDRAQIGEMS
KEIREVIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSATAEDLPYASFAGQQDTYLNIIGKEAILQHVRKCWASLFTER
AVMYRMQNGFEHNQVSICIVVQKMVFPQASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSADNYKVKEGEIIDKMI
ATKKLAIYALKEGGTETKQNDPVQQKIQTLSDQQILQLAQIGRQIEAYFGCPQDIEWCLVDNAFYIVQSRPITTLYPIPE
ENDEGNHVYISVGHQQMMTDAMKPLGLSFFLLTTNAPMREAGGRLFVDATQQLALPASRDYLINTLGKSDPLVRDALTTI
IERDDFITLLPDEEKKEKSASKSKLPVSSQLEIENDPAIVTELIRNSEASFEELKQNMQLKSGVDVFDFILEDIQQLKKV
LFNPQSIAVIMVGMNASTWINEKMEKWLGEKNAADTLSQSVQNNITSEMGLALMDVADAIRPYPEVITYLQHVEDDSFLD
ELVQYKGGEKARDAIVAFLNKYGMRCSGEIDITKTRWSEKPTTIIPMILNNIRDFEAGASKQKFEKGLQEALNKEEELVD
RLQHLPDGKQKIEETKRMIRNIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVIHGVDDIYYLTFEELHEVVRT
KKLNYDLIHKQKNDYKLYEKLTPPRIMTSDGEIITGKYKRENLPVDAIAGLPVSSGLVEGRARVILNMEDANLEEGDILV
TAFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKLIKDGQRIRVHGTEGYIEVL
>Mature_868_residues
SSFVLDFQEIEKTQLSLVGGKGLNLGALSNMQEIQVPEGFCVTTAGYEKAIEQNEGLQTLLQQLTKLKKEDRAQIGEMSK
EIREVIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSATAEDLPYASFAGQQDTYLNIIGKEAILQHVRKCWASLFTERA
VMYRMQNGFEHNQVSICIVVQKMVFPQASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSADNYKVKEGEIIDKMIA
TKKLAIYALKEGGTETKQNDPVQQKIQTLSDQQILQLAQIGRQIEAYFGCPQDIEWCLVDNAFYIVQSRPITTLYPIPEE
NDEGNHVYISVGHQQMMTDAMKPLGLSFFLLTTNAPMREAGGRLFVDATQQLALPASRDYLINTLGKSDPLVRDALTTII
ERDDFITLLPDEEKKEKSASKSKLPVSSQLEIENDPAIVTELIRNSEASFEELKQNMQLKSGVDVFDFILEDIQQLKKVL
FNPQSIAVIMVGMNASTWINEKMEKWLGEKNAADTLSQSVQNNITSEMGLALMDVADAIRPYPEVITYLQHVEDDSFLDE
LVQYKGGEKARDAIVAFLNKYGMRCSGEIDITKTRWSEKPTTIIPMILNNIRDFEAGASKQKFEKGLQEALNKEEELVDR
LQHLPDGKQKIEETKRMIRNIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVIHGVDDIYYLTFEELHEVVRTK
KLNYDLIHKQKNDYKLYEKLTPPRIMTSDGEIITGKYKRENLPVDAIAGLPVSSGLVEGRARVILNMEDANLEEGDILVT
AFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKLIKDGQRIRVHGTEGYIEVL

Specific function: Might catalyze the phosphorylation of pyruvate to phosphoenolpyruvate (Potential) [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=335, Percent_Identity=34.6268656716418, Blast_Score=172, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17564524, Length=301, Percent_Identity=31.8936877076412, Blast_Score=127, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR002192 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N [H]

EC number: 2.7.9.2

Molecular weight: Translated: 97546; Mature: 97415

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFVLDFQEIEKTQLSLVGGKGLNLGALSNMQEIQVPEGFCVTTAGYEKAIEQNEGLQT
CCCCCHHHHHHHHHHHHEECCCCCCCHHHCCCHHCCCCCCEEEECCCHHHHHHHCCCHHH
LLQQLTKLKKEDRAQIGEMSKEIREVIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCC
AEDLPYASFAGQQDTYLNIIGKEAILQHVRKCWASLFTERAVMYRMQNGFEHNQVSICIV
CCCCCCHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
VQKMVFPQASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSADNYKVKEGEIIDKMI
EHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEECCCHHHHHHH
ATKKLAIYALKEGGTETKQNDPVQQKIQTLSDQQILQLAQIGRQIEAYFGCPQDIEWCLV
HHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
DNAFYIVQSRPITTLYPIPEENDEGNHVYISVGHQQMMTDAMKPLGLSFFLLTTNAPMRE
CCEEEEEECCCCEEEECCCCCCCCCCEEEEEECHHHHHHHHHHHCCCEEEEEECCCCHHH
AGGRLFVDATQQLALPASRDYLINTLGKSDPLVRDALTTIIERDDFITLLPDEEKKEKSA
CCCEEEEECHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHH
SKSKLPVSSQLEIENDPAIVTELIRNSEASFEELKQNMQLKSGVDVFDFILEDIQQLKKV
HHCCCCCCCCEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LFNPQSIAVIMVGMNASTWINEKMEKWLGEKNAADTLSQSVQNNITSEMGLALMDVADAI
HCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RPYPEVITYLQHVEDDSFLDELVQYKGGEKARDAIVAFLNKYGMRCSGEIDITKTRWSEK
CCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
PTTIIPMILNNIRDFEAGASKQKFEKGLQEALNKEEELVDRLQHLPDGKQKIEETKRMIR
CCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHH
NIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVIHGVDDIYYLTFEELHEVVRT
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEHHHHHHHHHH
KKLNYDLIHKQKNDYKLYEKLTPPRIMTSDGEIITGKYKRENLPVDAIAGLPVSSGLVEG
HCCCHHHHHCCCCCHHHHHHCCCCCEEECCCCEEECCCCCCCCCHHHHCCCCCCCCHHCC
RARVILNMEDANLEEGDILVTAFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPA
CEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCE
VVGVENATKLIKDGQRIRVHGTEGYIEVL
EEECHHHHHHHHCCCEEEEECCCCEEEEC
>Mature Secondary Structure 
SSFVLDFQEIEKTQLSLVGGKGLNLGALSNMQEIQVPEGFCVTTAGYEKAIEQNEGLQT
CCCCHHHHHHHHHHHHEECCCCCCCHHHCCCHHCCCCCCEEEECCCHHHHHHHCCCHHH
LLQQLTKLKKEDRAQIGEMSKEIREVIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCC
AEDLPYASFAGQQDTYLNIIGKEAILQHVRKCWASLFTERAVMYRMQNGFEHNQVSICIV
CCCCCCHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
VQKMVFPQASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSADNYKVKEGEIIDKMI
EHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEECCCHHHHHHH
ATKKLAIYALKEGGTETKQNDPVQQKIQTLSDQQILQLAQIGRQIEAYFGCPQDIEWCLV
HHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
DNAFYIVQSRPITTLYPIPEENDEGNHVYISVGHQQMMTDAMKPLGLSFFLLTTNAPMRE
CCEEEEEECCCCEEEECCCCCCCCCCEEEEEECHHHHHHHHHHHCCCEEEEEECCCCHHH
AGGRLFVDATQQLALPASRDYLINTLGKSDPLVRDALTTIIERDDFITLLPDEEKKEKSA
CCCEEEEECHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHH
SKSKLPVSSQLEIENDPAIVTELIRNSEASFEELKQNMQLKSGVDVFDFILEDIQQLKKV
HHCCCCCCCCEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LFNPQSIAVIMVGMNASTWINEKMEKWLGEKNAADTLSQSVQNNITSEMGLALMDVADAI
HCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RPYPEVITYLQHVEDDSFLDELVQYKGGEKARDAIVAFLNKYGMRCSGEIDITKTRWSEK
CCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
PTTIIPMILNNIRDFEAGASKQKFEKGLQEALNKEEELVDRLQHLPDGKQKIEETKRMIR
CCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHH
NIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVIHGVDDIYYLTFEELHEVVRT
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEHHHHHHHHHH
KKLNYDLIHKQKNDYKLYEKLTPPRIMTSDGEIITGKYKRENLPVDAIAGLPVSSGLVEG
HCCCHHHHHCCCCCHHHHHHCCCCCEEECCCCEEECCCCCCCCCHHHHCCCCCCCCHHCC
RARVILNMEDANLEEGDILVTAFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPA
CEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCE
VVGVENATKLIKDGQRIRVHGTEGYIEVL
EEECHHHHHHHHCCCEEEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10.5 {phosphate}} 0.028 {ATP}} 0.083 {pyruvate}} [C]

Substrates: ATP; pyruvate; H2O

Specific reaction: ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 7704256 [H]