BacMap

Definition	Bacillus cereus AH820, complete genome.
Accession	NC_011773
Length	5,302,683

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The map label for this gene is crt [H]

Identifier: 218904158

GI number: 218904158

Start: 2882065

End: 2882844

Strand: Reverse

Name: crt [H]

Synonym: BCAH820_3042

Alternate gene names: 218904158

Gene position: 2882844-2882065 (Counterclockwise)

Preceding gene: 218904159

Following gene: 218904157

Centisome position: 54.37

GC content: 37.56

Gene sequence:

>780_bases
ATGAATTATGAGTTTTTAATATGTGATATTGAAAATAAAGTTGCGATTGTTACGATTAATCGACCACCTGTAAATCCGCT
AAATACACAAGTGTTTCATGAACTTTCCAGTCTATTTAATGAACTAGAAGTAAATGAAGATGTACGAGTAATCGTTCTTA
CAGGAAGCGGAAGGAAAGCATTTGTCGCAGGTGCTGATATTAATGAAATGGCAGCACTTGACCTTGTCGGTGTAAACAAA
ATGAATAAAACTTCACGAACGGTATTTAGCAAAATTGAAAATATGTCAAAACCAGTTATTGCTGCGATAAATGGATTAGC
TCTTGGCGGTGGTTTTGAACTTGCACTCGCATGTGACTTACGCATTTGTTCGGATCAAGCAAAATTCGCTTTTCCAGAAG
TAGGACTTGGAATTATTCCTGGAGGCGGAGGGACACAGCGTCTTCAAAAGATTGTCGGCCAAGGTATAGCGAAAGAATTA
CTATATTTCGGAGACATGTTTGATGCGAAGCGTGCGTTTGATTTACATATTATCAACAAAATAGTACCTGCAGAAGAGTT
ATTTGAAGCAGCAAAGGAATGGGCATATAAATTGGCAAAAAAACCTCCAGTAGCACTTCAAATGTTAAAAACAGCGGTAA
ATGTAGGAAGTAATGCTGACCTTGAAACAGGCTTAATCATCGAAAGCACCTGCTTTGGGAATGCTTTCGCAACAGAGGAT
CGTAAAGAAGGATTACAAGCATTCGTTGAAAAAAGAAAACCAGTTTATTTAGGGAAATAA

Upstream 100 bases:

>100_bases
GCCGCCGCTCCTTTTAAAACAGTTAGTTAGAGCAGGTAGACATGGTAAAAAAACAGGCGCAGGTTTTTATGATTATGAAT
AAAGAATGGGGGATACAGTT

Downstream 100 bases:

>100_bases
TGAGGTGAAAAGGATATGAAAGAAATTGTTTTATTAGAGGGAGCAAGGACTCCGTTCGCTGCGATTTCTGGATCGTTTCG
AGGATTAAATGCAACAGATT

Product: 3-hydroxybutyryl-CoA dehydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MNYEFLICDIENKVAIVTINRPPVNPLNTQVFHELSSLFNELEVNEDVRVIVLTGSGRKAFVAGADINEMAALDLVGVNK
MNKTSRTVFSKIENMSKPVIAAINGLALGGGFELALACDLRICSDQAKFAFPEVGLGIIPGGGGTQRLQKIVGQGIAKEL
LYFGDMFDAKRAFDLHIINKIVPAEELFEAAKEWAYKLAKKPPVALQMLKTAVNVGSNADLETGLIIESTCFGNAFATED
RKEGLQAFVEKRKPVYLGK

Sequences:

>Translated_259_residues
MNYEFLICDIENKVAIVTINRPPVNPLNTQVFHELSSLFNELEVNEDVRVIVLTGSGRKAFVAGADINEMAALDLVGVNK
MNKTSRTVFSKIENMSKPVIAAINGLALGGGFELALACDLRICSDQAKFAFPEVGLGIIPGGGGTQRLQKIVGQGIAKEL
LYFGDMFDAKRAFDLHIINKIVPAEELFEAAKEWAYKLAKKPPVALQMLKTAVNVGSNADLETGLIIESTCFGNAFATED
RKEGLQAFVEKRKPVYLGK
>Mature_259_residues
MNYEFLICDIENKVAIVTINRPPVNPLNTQVFHELSSLFNELEVNEDVRVIVLTGSGRKAFVAGADINEMAALDLVGVNK
MNKTSRTVFSKIENMSKPVIAAINGLALGGGFELALACDLRICSDQAKFAFPEVGLGIIPGGGGTQRLQKIVGQGIAKEL
LYFGDMFDAKRAFDLHIINKIVPAEELFEAAKEWAYKLAKKPPVALQMLKTAVNVGSNADLETGLIIESTCFGNAFATED
RKEGLQAFVEKRKPVYLGK

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=40.625, Blast_Score=175, Evalue=4e-44,
Organism=Homo sapiens, GI4502327, Length=255, Percent_Identity=40.3921568627451, Blast_Score=147, Evalue=7e-36,
Organism=Homo sapiens, GI20127408, Length=205, Percent_Identity=37.0731707317073, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI68989263, Length=211, Percent_Identity=32.2274881516588, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI70995211, Length=268, Percent_Identity=29.8507462686567, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI150378541, Length=248, Percent_Identity=29.0322580645161, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI31542718, Length=243, Percent_Identity=30.0411522633745, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI213417737, Length=235, Percent_Identity=28.0851063829787, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI157694516, Length=235, Percent_Identity=28.0851063829787, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI157694520, Length=204, Percent_Identity=28.921568627451, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI62530384, Length=242, Percent_Identity=25.6198347107438, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI295842266, Length=240, Percent_Identity=23.75, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI45643119, Length=216, Percent_Identity=26.3888888888889, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI260275230, Length=216, Percent_Identity=26.3888888888889, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI260274832, Length=216, Percent_Identity=26.3888888888889, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI37594469, Length=150, Percent_Identity=28, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI37594471, Length=150, Percent_Identity=28, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI261878539, Length=115, Percent_Identity=33.9130434782609, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=39.1472868217054, Blast_Score=183, Evalue=9e-48,
Organism=Escherichia coli, GI221142681, Length=247, Percent_Identity=36.0323886639676, Blast_Score=150, Evalue=6e-38,
Organism=Escherichia coli, GI1788597, Length=252, Percent_Identity=32.9365079365079, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1788682, Length=191, Percent_Identity=35.6020942408377, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=33.0798479087452, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=29.4339622641509, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1790281, Length=187, Percent_Identity=34.7593582887701, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=40.4858299595142, Blast_Score=179, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=40.9836065573771, Blast_Score=178, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17540714, Length=222, Percent_Identity=35.5855855855856, Blast_Score=154, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17558304, Length=177, Percent_Identity=35.0282485875706, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17508953, Length=147, Percent_Identity=39.4557823129252, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25144276, Length=147, Percent_Identity=39.4557823129252, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17508951, Length=147, Percent_Identity=39.4557823129252, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=249, Percent_Identity=28.1124497991968, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=257, Percent_Identity=26.8482490272374, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=257, Percent_Identity=28.0155642023346, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=252, Percent_Identity=26.5873015873016, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25144160, Length=197, Percent_Identity=25.3807106598985, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=197, Percent_Identity=25.3807106598985, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=183, Percent_Identity=27.8688524590164, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=192, Percent_Identity=26.0416666666667, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=39.5348837209302, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=39.5348837209302, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=38.2470119521912, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI19920382, Length=256, Percent_Identity=31.25, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI19921018, Length=205, Percent_Identity=32.1951219512195, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24654903, Length=188, Percent_Identity=28.1914893617021, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21357171, Length=256, Percent_Identity=27.734375, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583077, Length=184, Percent_Identity=36.4130434782609, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24583079, Length=184, Percent_Identity=36.4130434782609, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24583165, Length=212, Percent_Identity=29.7169811320755, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19921000, Length=184, Percent_Identity=36.4130434782609, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24650670, Length=226, Percent_Identity=27.8761061946903, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19922422, Length=254, Percent_Identity=25.1968503937008, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45550169, Length=205, Percent_Identity=27.3170731707317, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28571730, Length=317, Percent_Identity=25.8675078864353, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571729, Length=317, Percent_Identity=25.8675078864353, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 28193; Mature: 28193

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYEFLICDIENKVAIVTINRPPVNPLNTQVFHELSSLFNELEVNEDVRVIVLTGSGRKA
CCCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEE
FVAGADINEMAALDLVGVNKMNKTSRTVFSKIENMSKPVIAAINGLALGGGFELALACDL
EEECCCCHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCHHHHHCCEEECCCEEEEEEEEE
RICSDQAKFAFPEVGLGIIPGGGGTQRLQKIVGQGIAKELLYFGDMFDAKRAFDLHIINK
EECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHH
IVPAEELFEAAKEWAYKLAKKPPVALQMLKTAVNVGSNADLETGLIIESTCFGNAFATED
HCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCH
RKEGLQAFVEKRKPVYLGK
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNYEFLICDIENKVAIVTINRPPVNPLNTQVFHELSSLFNELEVNEDVRVIVLTGSGRKA
CCCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEE
FVAGADINEMAALDLVGVNKMNKTSRTVFSKIENMSKPVIAAINGLALGGGFELALACDL
EEECCCCHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCHHHHHCCEEECCCEEEEEEEEE
RICSDQAKFAFPEVGLGIIPGGGGTQRLQKIVGQGIAKELLYFGDMFDAKRAFDLHIINK
EECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHH
IVPAEELFEAAKEWAYKLAKKPPVALQMLKTAVNVGSNADLETGLIIESTCFGNAFATED
HCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCH
RKEGLQAFVEKRKPVYLGK
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]