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The map label for this gene is mhpA [H]

Identifier: 218904154

GI number: 218904154

Start: 2877493

End: 2878722

Strand: Reverse

Name: mhpA [H]

Synonym: BCAH820_3038

Alternate gene names: 218904154

Gene position: 2878722-2877493 (Counterclockwise)

Preceding gene: 218904155

Following gene: 218904153

Centisome position: 54.29

GC content: 41.22

Gene sequence:

>1230_bases
ATGGGAAATGAAAAAAAATATGATGCCATAGTAGTTGGGGCGGGACCAGTCGGGCTTGTAGCAGGACTTGCATTACAAAA
GAAGGGGATATCAACTTTAGTAATTGAGGCAGATTCATATGGAAGACCACGCCCGGGAAGTAGAGCAATTTATTTGCATA
CCGCTTCGTTAAAACTACTAGAGGAAACTGCAGAAGGCCTCGGTTTTACACTTGCTCGTAACGGAATTATTTGGCCTGTA
AAACGAACGTTTTATAAAGGGAAAGAAGTATATGTAAGAGATTATGGTAAGGATGAAAACCTTAAGTTTAATCGATTGCC
TCATTTTACAGCACTACATCAAGACGAAATCGAAAAACATATGTATGAGGCGTGTATAAAAGAAGGTGTTGAATTCCTTT
GGGATGCCCCGGTCAAGAAGTTACATATTGCAGATTCCGGAGTAGAACTAACAATAACAAATGATGAAATCTTAAAGGCG
CAGTATGTAATTGGATGTGATGGAGCACGTTCCATCGTAAGAGAAGAAGCAGGATTAACATTTGAAGGCCCACGTACAGC
AGATACTTTTATCGTAGTAGATGCAAAAGAAGATGAAACAGACCCACTTCCTTTAGAAAGAATATTCCATTATCAGCATC
CAGCTATGGAAGGAAGAAATGTAATGTTTGTTCCGTTTAAAGGCGGGTGGCGTATCGATTTACAACTGCTTGAATCAGAT
AATCCAGATGATTATACAAATATGGAAAGTGTAAAGAAATGGTTGCCGAAAGTCATGGATGCTAAATATGCGGAGAGAAT
TACGTGGGTGTCTTCTTATCGTTTCCATCAAGTAGTGGCGAATTCGTTTACTGACGAAAAACGTCGTGTTCTTCTAGCTG
GAGAAGCAGCACACTTATTTGCCCCATTCGGAGCACGTGGATTAAATTCTGGTATTCCAGATGCTGTTCTTGCTGCAAGA
GGAATTGAGAAAGCACTACATTCTCATTCTGAAGAAGAGCGAGTAGAAGCAATTGAAGCAGCTGCAAAAGAACGCCGAAT
TGCAGCGCAGTGGAACCGCGATGCATCAACTACAGCACTTCACCATCTTCAAGGGAGTTCTTCTGAAATGAAAATGAAAC
GTGACATTGCTGCTTCACTGGTATCTATCGTTCCGAGGCTTGGAAGATGGCTTGACGAAGGCCCATATGGACCGAAATTT
GGACCTCCTGAACTGACGACAAAATATTAA

Upstream 100 bases:

>100_bases
ATTCGGCAGAAGATGATGCCAGAGATAAAAACTATAGAAAGTTAAAGAAGAACGAGTAAGGATACCGTAACGAGAAATTG
TTAATGAGGGAGAGATAGCG

Downstream 100 bases:

>100_bases
AATGATAAAAAATTAAATAAGTAATGTGAAAGGAGCGATAAGAAATGGCTTTGTCTCAATTCATAAACGAAGAAAAGTAT
GGTTCTCACGCCCGTTCTAC

Product: monooxygenase, FAD-binding

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MGNEKKYDAIVVGAGPVGLVAGLALQKKGISTLVIEADSYGRPRPGSRAIYLHTASLKLLEETAEGLGFTLARNGIIWPV
KRTFYKGKEVYVRDYGKDENLKFNRLPHFTALHQDEIEKHMYEACIKEGVEFLWDAPVKKLHIADSGVELTITNDEILKA
QYVIGCDGARSIVREEAGLTFEGPRTADTFIVVDAKEDETDPLPLERIFHYQHPAMEGRNVMFVPFKGGWRIDLQLLESD
NPDDYTNMESVKKWLPKVMDAKYAERITWVSSYRFHQVVANSFTDEKRRVLLAGEAAHLFAPFGARGLNSGIPDAVLAAR
GIEKALHSHSEEERVEAIEAAAKERRIAAQWNRDASTTALHHLQGSSSEMKMKRDIAASLVSIVPRLGRWLDEGPYGPKF
GPPELTTKY

Sequences:

>Translated_409_residues
MGNEKKYDAIVVGAGPVGLVAGLALQKKGISTLVIEADSYGRPRPGSRAIYLHTASLKLLEETAEGLGFTLARNGIIWPV
KRTFYKGKEVYVRDYGKDENLKFNRLPHFTALHQDEIEKHMYEACIKEGVEFLWDAPVKKLHIADSGVELTITNDEILKA
QYVIGCDGARSIVREEAGLTFEGPRTADTFIVVDAKEDETDPLPLERIFHYQHPAMEGRNVMFVPFKGGWRIDLQLLESD
NPDDYTNMESVKKWLPKVMDAKYAERITWVSSYRFHQVVANSFTDEKRRVLLAGEAAHLFAPFGARGLNSGIPDAVLAAR
GIEKALHSHSEEERVEAIEAAAKERRIAAQWNRDASTTALHHLQGSSSEMKMKRDIAASLVSIVPRLGRWLDEGPYGPKF
GPPELTTKY
>Mature_408_residues
GNEKKYDAIVVGAGPVGLVAGLALQKKGISTLVIEADSYGRPRPGSRAIYLHTASLKLLEETAEGLGFTLARNGIIWPVK
RTFYKGKEVYVRDYGKDENLKFNRLPHFTALHQDEIEKHMYEACIKEGVEFLWDAPVKKLHIADSGVELTITNDEILKAQ
YVIGCDGARSIVREEAGLTFEGPRTADTFIVVDAKEDETDPLPLERIFHYQHPAMEGRNVMFVPFKGGWRIDLQLLESDN
PDDYTNMESVKKWLPKVMDAKYAERITWVSSYRFHQVVANSFTDEKRRVLLAGEAAHLFAPFGARGLNSGIPDAVLAARG
IEKALHSHSEEERVEAIEAAAKERRIAAQWNRDASTTALHHLQGSSSEMKMKRDIAASLVSIVPRLGRWLDEGPYGPKFG
PPELTTKY

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=393, Percent_Identity=24.1730279898219, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 45776; Mature: 45644

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNEKKYDAIVVGAGPVGLVAGLALQKKGISTLVIEADSYGRPRPGSRAIYLHTASLKLL
CCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEEHHHHHH
EETAEGLGFTLARNGIIWPVKRTFYKGKEVYVRDYGKDENLKFNRLPHFTALHQDEIEKH
HHHHHCCCEEEECCCEEEECHHHHHCCCEEEEEECCCCCCEEECCCCCHHHHHHHHHHHH
MYEACIKEGVEFLWDAPVKKLHIADSGVELTITNDEILKAQYVIGCDGARSIVREEAGLT
HHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCEEEEEEEEECCHHHHHHHHHCCCE
FEGPRTADTFIVVDAKEDETDPLPLERIFHYQHPAMEGRNVMFVPFKGGWRIDLQLLESD
ECCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEEEECC
NPDDYTNMESVKKWLPKVMDAKYAERITWVSSYRFHQVVANSFTDEKRRVLLAGEAAHLF
CCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHEE
APFGARGLNSGIPDAVLAARGIEKALHSHSEEERVEAIEAAAKERRIAAQWNRDASTTAL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
HHLQGSSSEMKMKRDIAASLVSIVPRLGRWLDEGPYGPKFGPPELTTKY
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GNEKKYDAIVVGAGPVGLVAGLALQKKGISTLVIEADSYGRPRPGSRAIYLHTASLKLL
CCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEEHHHHHH
EETAEGLGFTLARNGIIWPVKRTFYKGKEVYVRDYGKDENLKFNRLPHFTALHQDEIEKH
HHHHHCCCEEEECCCEEEECHHHHHCCCEEEEEECCCCCCEEECCCCCHHHHHHHHHHHH
MYEACIKEGVEFLWDAPVKKLHIADSGVELTITNDEILKAQYVIGCDGARSIVREEAGLT
HHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCEEEEEEEEECCHHHHHHHHHCCCE
FEGPRTADTFIVVDAKEDETDPLPLERIFHYQHPAMEGRNVMFVPFKGGWRIDLQLLESD
ECCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEEEECC
NPDDYTNMESVKKWLPKVMDAKYAERITWVSSYRFHQVVANSFTDEKRRVLLAGEAAHLF
CCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHEE
APFGARGLNSGIPDAVLAARGIEKALHSHSEEERVEAIEAAAKERRIAAQWNRDASTTAL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
HHLQGSSSEMKMKRDIAASLVSIVPRLGRWLDEGPYGPKFGPPELTTKY
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA