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The map label for this gene is pbpE [H]

Identifier: 218903784

GI number: 218903784

Start: 2537882

End: 2538928

Strand: Direct

Name: pbpE [H]

Synonym: BCAH820_2668

Alternate gene names: 218903784

Gene position: 2537882-2538928 (Clockwise)

Preceding gene: 218903783

Following gene: 218903785

Centisome position: 47.86

GC content: 32.47

Gene sequence:

>1047_bases
TTGATAAACGAGGGTGATTTTGTAATGAAAACTAAAGATCAAATAGATAACATTGTAAAAGAAACATATCGTCATATAGA
TTTCTCTGGAGTAATTTTAGTGAAAGAAAAAAAGGGCATAGTCTATGAAGAGGCGTTTGGTTATGCAAATCGAAATGAGT
GTATAAACAATACGCGACAAACTAGGTTTGGAATTGCTTCTGGGTGTAAAATATTCACAGCTATCGGTATCTGTCAACTT
GTTGAAAATGGGGTAATTACTTTTCAAACAAAGTTAAAAGAGTGTTTAAGTGTTAACTTTCCAAACTTTAATGAAGATAT
TACAATACATCACCTTTTGACGCATAGTTCGGGTATTCCAGATTATTTTGATGAAAGTATTATGGATAACTTCGAAGATC
TTTGGAAACAAACACCTATGTATTTATTAAAAAGTTTAAAAGACTTTTTACCATTATTTCAAAACAGAGATATGATGTAT
GCACCAGGAAGTAAGTTTCACTACAACAATGCAGGTTTTATCATACTGGGATTAATAATCGAAGAACAGACAGGACTTAC
ATTTACGGAGTATATAGAAAAAAACATATTTAATCCAATTGGTATGAATCATTCGGGCTATTTTTCTTTAGATAGATTAC
CAAGGCAGACAGCTCTCGGATATATAAAAGATGAAATTAGCCAGACATGGAGAACAAATGCTTACTCTATTCCGATAAAA
GGTGGTTCTGATGGTGGAGCTTTTATTACTGCGTCAGACATGCTGAAGTTTTGGGAAGCGTTATTTAACTATGAAATTAT
AAGCCAAGAATATACAAAAATACTTTTAACTCCTCACATCCAAGTAAATAATAATCAATCTTATGGCTATGGAATATGGA
TTGAAACAAGAGAAAACAAGATATTCAAATATCATGTAATGGGATATGACCCAGGTGTCAGTTTTCGCTCGGCCGTATAT
CCAGACTTAGGAATTACATTAGTTATTCCGTCAAATAAAGGAGCAGGACCCGAAAAATTAATGATAGAAATAGAAGGGAG
TTTTTAA

Upstream 100 bases:

>100_bases
TACATTCGAAATAATTCTCTTAGTTTTGATTTAAATCTTACGATGTTCAATAGGAGGGTTATCCCTCCTATATATGGAAA
TATAAGAAAGGAGAACAATA

Downstream 100 bases:

>100_bases
TTATATGGATATAGAAATTAGAGCGTATAGAAAAGAGAATGAAGCTGGTTGGGTACGTTGCCGTGTGTTATCTTTTTTAG
ATACAGCTTATTACGATAAT

Product: penicillin-binding protein

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MINEGDFVMKTKDQIDNIVKETYRHIDFSGVILVKEKKGIVYEEAFGYANRNECINNTRQTRFGIASGCKIFTAIGICQL
VENGVITFQTKLKECLSVNFPNFNEDITIHHLLTHSSGIPDYFDESIMDNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMY
APGSKFHYNNAGFIILGLIIEEQTGLTFTEYIEKNIFNPIGMNHSGYFSLDRLPRQTALGYIKDEISQTWRTNAYSIPIK
GGSDGGAFITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENKIFKYHVMGYDPGVSFRSAVY
PDLGITLVIPSNKGAGPEKLMIEIEGSF

Sequences:

>Translated_348_residues
MINEGDFVMKTKDQIDNIVKETYRHIDFSGVILVKEKKGIVYEEAFGYANRNECINNTRQTRFGIASGCKIFTAIGICQL
VENGVITFQTKLKECLSVNFPNFNEDITIHHLLTHSSGIPDYFDESIMDNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMY
APGSKFHYNNAGFIILGLIIEEQTGLTFTEYIEKNIFNPIGMNHSGYFSLDRLPRQTALGYIKDEISQTWRTNAYSIPIK
GGSDGGAFITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENKIFKYHVMGYDPGVSFRSAVY
PDLGITLVIPSNKGAGPEKLMIEIEGSF
>Mature_348_residues
MINEGDFVMKTKDQIDNIVKETYRHIDFSGVILVKEKKGIVYEEAFGYANRNECINNTRQTRFGIASGCKIFTAIGICQL
VENGVITFQTKLKECLSVNFPNFNEDITIHHLLTHSSGIPDYFDESIMDNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMY
APGSKFHYNNAGFIILGLIIEEQTGLTFTEYIEKNIFNPIGMNHSGYFSLDRLPRQTALGYIKDEISQTWRTNAYSIPIK
GGSDGGAFITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENKIFKYHVMGYDPGVSFRSAVY
PDLGITLVIPSNKGAGPEKLMIEIEGSF

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI115533026, Length=275, Percent_Identity=26.1818181818182, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17550934, Length=180, Percent_Identity=28.3333333333333, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17565630, Length=289, Percent_Identity=23.8754325259516, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 39800; Mature: 39800

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINEGDFVMKTKDQIDNIVKETYRHIDFSGVILVKEKKGIVYEEAFGYANRNECINNTRQ
CCCCCCEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEHHHCCCCCCHHHHCCCHH
TRFGIASGCKIFTAIGICQLVENGVITFQTKLKECLSVNFPNFNEDITIHHLLTHSSGIP
HHHCHHCCHHHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCC
DYFDESIMDNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMYAPGSKFHYNNAGFIILGLII
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCEEEEEEEE
EEQTGLTFTEYIEKNIFNPIGMNHSGYFSLDRLPRQTALGYIKDEISQTWRTNAYSIPIK
ECCCCCHHHHHHHHHCCCCCCCCCCCCEEHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEC
GGSDGGAFITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENK
CCCCCCEEEEHHHHHHHHHHHHCHHHHCCCCEEEEEECEEEECCCCCCEEEEEEEECCCE
IFKYHVMGYDPGVSFRSAVYPDLGITLVIPSNKGAGPEKLMIEIEGSF
EEEEEEEEECCCCCHHHHCCCCCCEEEEEECCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MINEGDFVMKTKDQIDNIVKETYRHIDFSGVILVKEKKGIVYEEAFGYANRNECINNTRQ
CCCCCCEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEHHHCCCCCCHHHHCCCHH
TRFGIASGCKIFTAIGICQLVENGVITFQTKLKECLSVNFPNFNEDITIHHLLTHSSGIP
HHHCHHCCHHHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCC
DYFDESIMDNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMYAPGSKFHYNNAGFIILGLII
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCEEEEEEEE
EEQTGLTFTEYIEKNIFNPIGMNHSGYFSLDRLPRQTALGYIKDEISQTWRTNAYSIPIK
ECCCCCHHHHHHHHHCCCCCCCCCCCCEEHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEC
GGSDGGAFITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENK
CCCCCCEEEEHHHHHHHHHHHHCHHHHCCCCEEEEEECEEEECCCCCCEEEEEEEECCCE
IFKYHVMGYDPGVSFRSAVYPDLGITLVIPSNKGAGPEKLMIEIEGSF
EEEEEEEEECCCCCHHHHCCCCCCEEEEEECCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]