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The map label for this gene is ddl [H]

Identifier: 218903743

GI number: 218903743

Start: 2498266

End: 2499180

Strand: Direct

Name: ddl [H]

Synonym: BCAH820_2627

Alternate gene names: 218903743

Gene position: 2498266-2499180 (Clockwise)

Preceding gene: 218903742

Following gene: 218903744

Centisome position: 47.11

GC content: 34.32

Gene sequence:

>915_bases
ATGAGAATTGGCGTTATTATGGGAGGAGTATCCTCTGAAAAACAAGTATCAATTATGACTGGAAACGAAATGATTGCAAA
TTTAGATAAAAATAAATATGAAATCGTACCAATTACGTTAAATGAAAAAATGGATTTAATCGAAAAAGCGAAAGACATTG
ATTTTGCGCTGCTCGCATTACATGGAAAATACGGAGAAGATGGGACAGTTCAAGGAACGCTTGAAAGTTTAGGTATTCCT
TATAGCGGCAGCAATATGTTATCTAGCGGTATATGTATGGACAAAAATATTTCGAAAAAGATTTTACGTTATGAAGGAAT
AGAAACGCCAGATTGGATTGAACTTACAAAAATGGAGGATCTGAATTTTGATGAATTAGATAAGTTAGGATTTCCGTTAG
TAGTAAAACCAAACTCTGGTGGTTCAAGTGTTGGAGTAAAGATTGTCTACAATAAAGACGAATTAATTTCAATGCTGGAA
ACTGTGTTCGAATGGGATTCTGAAGTAGTAATAGAGAAATATATAAAAGGTGAAGAGATTACATGTTCCATTTTTGATGG
GAAACAGTTACCTATCATTTCAATTCGGCATGCGGCTGAGTTCTTTGATTACAATGCGAAATATGATGACGCTAGTACAA
TTGAAGAAGTTATCGAACTTCCAGCTGAACTTAAGGAACGTGTAAACAAAGCATCACTGGCGTGCTATAAAGCATTAAAA
TGTAGTGTTTATGCAAGGGTTGATATGATGGTGAAGGACGGTATTCCATATGTAATGGAGGTTAATACATTACCAGGGAT
GACACAAGCAAGTTTACTACCAAAAAGTGCAGATGCAGCGGGAATTCATTATAGTAAACTATTAGATATGATTATTGAAA
CTTCATTAAGAGTAAGAAAAGAAGAAGGGTTTTAA

Upstream 100 bases:

>100_bases
ACATTTCGATTAGGATTTTCACGTCTGAAAGAAGAAGAAATAGTTCGGGGAATCAAAATAATCGGTGATACAATAAAAAA
TGAAATTTGGAGTTGAGGAA

Downstream 100 bases:

>100_bases
GTAACGGTATTATGAATACTTTTGACAGTAACAAGAAGTATGTTATGATGAATACGATACGAAAATAAAATATTTATCCA
CTAGGGGGGCCTATTATAGG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MRIGVIMGGVSSEKQVSIMTGNEMIANLDKNKYEIVPITLNEKMDLIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIP
YSGSNMLSSGICMDKNISKKILRYEGIETPDWIELTKMEDLNFDELDKLGFPLVVKPNSGGSSVGVKIVYNKDELISMLE
TVFEWDSEVVIEKYIKGEEITCSIFDGKQLPIISIRHAAEFFDYNAKYDDASTIEEVIELPAELKERVNKASLACYKALK
CSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSADAAGIHYSKLLDMIIETSLRVRKEEGF

Sequences:

>Translated_304_residues
MRIGVIMGGVSSEKQVSIMTGNEMIANLDKNKYEIVPITLNEKMDLIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIP
YSGSNMLSSGICMDKNISKKILRYEGIETPDWIELTKMEDLNFDELDKLGFPLVVKPNSGGSSVGVKIVYNKDELISMLE
TVFEWDSEVVIEKYIKGEEITCSIFDGKQLPIISIRHAAEFFDYNAKYDDASTIEEVIELPAELKERVNKASLACYKALK
CSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSADAAGIHYSKLLDMIIETSLRVRKEEGF
>Mature_304_residues
MRIGVIMGGVSSEKQVSIMTGNEMIANLDKNKYEIVPITLNEKMDLIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIP
YSGSNMLSSGICMDKNISKKILRYEGIETPDWIELTKMEDLNFDELDKLGFPLVVKPNSGGSSVGVKIVYNKDELISMLE
TVFEWDSEVVIEKYIKGEEITCSIFDGKQLPIISIRHAAEFFDYNAKYDDASTIEEVIELPAELKERVNKASLACYKALK
CSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSADAAGIHYSKLLDMIIETSLRVRKEEGF

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=301, Percent_Identity=36.5448504983389, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1786579, Length=349, Percent_Identity=29.512893982808, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 33852; Mature: 33852

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGVIMGGVSSEKQVSIMTGNEMIANLDKNKYEIVPITLNEKMDLIEKAKDIDFALLAL
CEEEEEECCCCCCCEEEEEECCHHEECCCCCCEEEEEEEECCHHHHHHHHCCCCEEEEEE
HGKYGEDGTVQGTLESLGIPYSGSNMLSSGICMDKNISKKILRYEGIETPDWIELTKMED
ECCCCCCCCEEEEHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCC
LNFDELDKLGFPLVVKPNSGGSSVGVKIVYNKDELISMLETVFEWDSEVVIEKYIKGEEI
CCHHHHHHCCCCEEEECCCCCCCEEEEEEECHHHHHHHHHHHHHCCCHHHHHHHCCCCEE
TCSIFDGKQLPIISIRHAAEFFDYNAKYDDASTIEEVIELPAELKERVNKASLACYKALK
EEEEECCCCCCEEEHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
CSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSADAAGIHYSKLLDMIIETSLRVRK
HEEEHEEEEHHHCCCCEEEEECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EEGF
CCCC
>Mature Secondary Structure
MRIGVIMGGVSSEKQVSIMTGNEMIANLDKNKYEIVPITLNEKMDLIEKAKDIDFALLAL
CEEEEEECCCCCCCEEEEEECCHHEECCCCCCEEEEEEEECCHHHHHHHHCCCCEEEEEE
HGKYGEDGTVQGTLESLGIPYSGSNMLSSGICMDKNISKKILRYEGIETPDWIELTKMED
ECCCCCCCCEEEEHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCC
LNFDELDKLGFPLVVKPNSGGSSVGVKIVYNKDELISMLETVFEWDSEVVIEKYIKGEEI
CCHHHHHHCCCCEEEECCCCCCCEEEEEEECHHHHHHHHHHHHHCCCHHHHHHHCCCCEE
TCSIFDGKQLPIISIRHAAEFFDYNAKYDDASTIEEVIELPAELKERVNKASLACYKALK
EEEEECCCCCCEEEHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
CSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSADAAGIHYSKLLDMIIETSLRVRK
HEEEHEEEEHHHCCCCEEEEECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EEGF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA