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The map label for this gene is cwlJ1 [H]

Identifier: 218903728

GI number: 218903728

Start: 2482009

End: 2482437

Strand: Reverse

Name: cwlJ1 [H]

Synonym: BCAH820_2612

Alternate gene names: 218903728

Gene position: 2482437-2482009 (Counterclockwise)

Preceding gene: 218903732

Following gene: 218903726

Centisome position: 46.81

GC content: 42.89

Gene sequence:

>429_bases
ATGCCCCTTATTCCATATAACGAAAGCGATGTTGACTTACTTGCTCGATTAATACGTGCTGAAGCTGAAGGCGAAGGCCG
ACAAGGTGAACAACTTGTTGGTTGCGTAGTAGTAAACCGTGTATTTTGTGATTGCTTAGATTTTAAACAACTTCGTTCTG
TACGTGATGCTGTATATCAAAGCCCCGGTGGATTTGAAGCAGTGCAATACGGATATTTTTATCAACGTGCTCGTGAATCA
GAAAAAGAAATCGCACGAAAAGTTTTACAAGGTGAGTGGCGCTGGCCAGCAAGATGGGCTCTTTGGTATTTCCGTCCAGT
TGGAGCTTGTCCGCCCGAATGGTATAACCAGCCGTTTGTAGGACAGTTTAAAAGCCATTGTTTTTATGAACCTAGTGGTA
ATGAATGTCCGAAATTGTATTCACGGTAG

Upstream 100 bases:

>100_bases
TTTCAGACAAGCTCCTCTTGAAATGTTAATTTTATGTGAAAAAGCACCAAGCTTATTTAAAAACATACACTCTTTACTAT
AACAGAAAGGTGGTATCACT

Downstream 100 bases:

>100_bases
TAAAATTTGGAACGCAAGTACTTAGAATTTTGGGTACTTGCGTTACTTTTAACTAAGTTCTAGTAGTCTAATAATGTGAT
GGATTTTTCGATATACTTTT

Product: cell wall hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 141

Protein sequence:

>142_residues
MPLIPYNESDVDLLARLIRAEAEGEGRQGEQLVGCVVVNRVFCDCLDFKQLRSVRDAVYQSPGGFEAVQYGYFYQRARES
EKEIARKVLQGEWRWPARWALWYFRPVGACPPEWYNQPFVGQFKSHCFYEPSGNECPKLYSR

Sequences:

>Translated_142_residues
MPLIPYNESDVDLLARLIRAEAEGEGRQGEQLVGCVVVNRVFCDCLDFKQLRSVRDAVYQSPGGFEAVQYGYFYQRARES
EKEIARKVLQGEWRWPARWALWYFRPVGACPPEWYNQPFVGQFKSHCFYEPSGNECPKLYSR
>Mature_141_residues
PLIPYNESDVDLLARLIRAEAEGEGRQGEQLVGCVVVNRVFCDCLDFKQLRSVRDAVYQSPGGFEAVQYGYFYQRARESE
KEIARKVLQGEWRWPARWALWYFRPVGACPPEWYNQPFVGQFKSHCFYEPSGNECPKLYSR

Specific function: Probable spore cortex-lytic enzyme involved in the depolymerization of cortex peptidoglycan during germination [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CwlJ family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105 [H]

Pfam domain/function: PF07486 Hydrolase_2 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 16597; Mature: 16466

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLIPYNESDVDLLARLIRAEAEGEGRQGEQLVGCVVVNRVFCDCLDFKQLRSVRDAVYQ
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHC
SPGGFEAVQYGYFYQRARESEKEIARKVLQGEWRWPARWALWYFRPVGACPPEWYNQPFV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHCCCCCC
GQFKSHCFYEPSGNECPKLYSR
HHHHHCEEECCCCCCCCCCCCC
>Mature Secondary Structure 
PLIPYNESDVDLLARLIRAEAEGEGRQGEQLVGCVVVNRVFCDCLDFKQLRSVRDAVYQ
CCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHC
SPGGFEAVQYGYFYQRARESEKEIARKVLQGEWRWPARWALWYFRPVGACPPEWYNQPFV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHCCCCCC
GQFKSHCFYEPSGNECPKLYSR
HHHHHCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704254; 9384377; 9515903; 11466292; 11807087; 12177332 [H]