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The map label for this gene is murE [H]

Identifier: 218903725

GI number: 218903725

Start: 2479087

End: 2480568

Strand: Direct

Name: murE [H]

Synonym: BCAH820_2609

Alternate gene names: 218903725

Gene position: 2479087-2480568 (Clockwise)

Preceding gene: 218903724

Following gene: 218903727

Centisome position: 46.75

GC content: 32.93

Gene sequence:

>1482_bases
TTGAAACTAAAAGAGCTGGCGAATTTGTTATTGGTTAAAGAAACTATTGGCGATATGAATGTAAAAATTACGGGATTAGA
AATGGATTCTAGAAAGATAACTAATGGTAACCTATTTATATGTGTATCAGGTATCGATGGTTTTCTTGAAGATCGACATC
AATTTGTTGAAGATGCAGTGAAAAATGGTGCTGTAGCGCTAATTGTAGAAAGAGATGTAAATATTGAGATACCTAAAATA
ATTGTTAAAGATGCTAGGTATGCTATGGCAGTTATTGCGTCACATTTCTATGGTTATCCATCTAATAAAATGAAGTTAAT
TGGAATTACAGGGACAAATGGGAAAACAACTACTTCTTATTTAATTGAAAAAATTTTAAGTGATTATGGATATCACACGG
GGCTCATGGGGAATAACGGAGTCAAAGTTGGTTCAAAATGGTATTCTACTGATATTAATACGCAAGAACCTCCAACACTT
CAACGGAATTTGCAAATGATGAAAAATCAAAATGCAGATTATTGTGTTATGGAAGTTACGTCACAAGGTTTACATATGGA
AAGAGTTAAAGGGTGTAACTTTAAAATAGCTGTTTTCACAAATTTAACACAAGATCATTTAGATTATCATGGAACATTTG
ATGAATATAAGCATGTAAAAAGTCTTCTTTTTGCGAGATTAGGAAATGATTTATCTACTACAGATAAAAAAATAGCTGTT
TTGAATGCAGATGATCCATCGGTAGAATACTTTAAAAAAATTACTTCAGCTGAGGTTATCACATATGGAATACATAATGG
GGCAGATGTTCAAGCGAAGAACATAACTTTAAGCCCAAAAGGAATTCGTTTTTTAGTTTCGTCCTTTAATGGAGAAATAG
AGGTTAGCCTTAATCTTGTGGGTCGTTTCAATGTTTATAATGCATTAGCAGCAATAACAGTTGCATTGGTTGAAGGTATT
CCGCTTACAAACATTAGTGATAGCTTATCTACCTTAAAGAGTATTGAAGGTAGAATGGAAATCATAGACGAAAATCAAGA
TTTCCTTGTAATAGTGGACTATGCTCACACACCGGATGCGTTAGAAAATGTTCTCTCTACAATTAGCGAGTTTTCAAAAG
GTAAAGTTATTACGGTTTTCGGTTGTGGTGGAGATAGAGACGCAAAAAAACGATCTATAATGGGTGGAATTGCGGGAAGT
TATAGCGATTTTGTTTTTATTACTTCTGACAATCCACGTTCGGAAGATCCGCAGGCAATAACGAAGGATATTGAAAAGGG
ATTTTCGCAAAATAATAATTTGAATTATAAAGTAGAAGTAGATCGTGAACTTGCAATAAATCATGCAATAAACATGGCAT
CTTCTAATGATATTGTTTTGATTGCGGGGAAAGGGCATGAAACGTATCAAATTTTAAAAGATTCAACTATTCATTTTGAT
GATAAAGAAAAAGCTCGACAAGCAATTATAAATAATAGATAG

Upstream 100 bases:

>100_bases
AAGGGTGATTAAATTTAGTTCGAAAAATATGGAATTTTAAACCATTGTTTTTATACAAAGTGGTAAACTTAATAAGGTAA
TATGTAAGGGGGCGGGGGAT

Downstream 100 bases:

>100_bases
ATTATCTTTTAGAAAAGACGTGATTAGATTAATTTGTGAATGGAGTGTTATGAATGCAAACAAGAAAAGCATATCCTTCC
AATTAATGAATTCATTAAAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MKLKELANLLLVKETIGDMNVKITGLEMDSRKITNGNLFICVSGIDGFLEDRHQFVEDAVKNGAVALIVERDVNIEIPKI
IVKDARYAMAVIASHFYGYPSNKMKLIGITGTNGKTTTSYLIEKILSDYGYHTGLMGNNGVKVGSKWYSTDINTQEPPTL
QRNLQMMKNQNADYCVMEVTSQGLHMERVKGCNFKIAVFTNLTQDHLDYHGTFDEYKHVKSLLFARLGNDLSTTDKKIAV
LNADDPSVEYFKKITSAEVITYGIHNGADVQAKNITLSPKGIRFLVSSFNGEIEVSLNLVGRFNVYNALAAITVALVEGI
PLTNISDSLSTLKSIEGRMEIIDENQDFLVIVDYAHTPDALENVLSTISEFSKGKVITVFGCGGDRDAKKRSIMGGIAGS
YSDFVFITSDNPRSEDPQAITKDIEKGFSQNNNLNYKVEVDRELAINHAINMASSNDIVLIAGKGHETYQILKDSTIHFD
DKEKARQAIINNR

Sequences:

>Translated_493_residues
MKLKELANLLLVKETIGDMNVKITGLEMDSRKITNGNLFICVSGIDGFLEDRHQFVEDAVKNGAVALIVERDVNIEIPKI
IVKDARYAMAVIASHFYGYPSNKMKLIGITGTNGKTTTSYLIEKILSDYGYHTGLMGNNGVKVGSKWYSTDINTQEPPTL
QRNLQMMKNQNADYCVMEVTSQGLHMERVKGCNFKIAVFTNLTQDHLDYHGTFDEYKHVKSLLFARLGNDLSTTDKKIAV
LNADDPSVEYFKKITSAEVITYGIHNGADVQAKNITLSPKGIRFLVSSFNGEIEVSLNLVGRFNVYNALAAITVALVEGI
PLTNISDSLSTLKSIEGRMEIIDENQDFLVIVDYAHTPDALENVLSTISEFSKGKVITVFGCGGDRDAKKRSIMGGIAGS
YSDFVFITSDNPRSEDPQAITKDIEKGFSQNNNLNYKVEVDRELAINHAINMASSNDIVLIAGKGHETYQILKDSTIHFD
DKEKARQAIINNR
>Mature_493_residues
MKLKELANLLLVKETIGDMNVKITGLEMDSRKITNGNLFICVSGIDGFLEDRHQFVEDAVKNGAVALIVERDVNIEIPKI
IVKDARYAMAVIASHFYGYPSNKMKLIGITGTNGKTTTSYLIEKILSDYGYHTGLMGNNGVKVGSKWYSTDINTQEPPTL
QRNLQMMKNQNADYCVMEVTSQGLHMERVKGCNFKIAVFTNLTQDHLDYHGTFDEYKHVKSLLFARLGNDLSTTDKKIAV
LNADDPSVEYFKKITSAEVITYGIHNGADVQAKNITLSPKGIRFLVSSFNGEIEVSLNLVGRFNVYNALAAITVALVEGI
PLTNISDSLSTLKSIEGRMEIIDENQDFLVIVDYAHTPDALENVLSTISEFSKGKVITVFGCGGDRDAKKRSIMGGIAGS
YSDFVFITSDNPRSEDPQAITKDIEKGFSQNNNLNYKVEVDRELAINHAINMASSNDIVLIAGKGHETYQILKDSTIHFD
DKEKARQAIINNR

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=474, Percent_Identity=36.2869198312236, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1786274, Length=365, Percent_Identity=27.6712328767123, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 54620; Mature: 54620

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKELANLLLVKETIGDMNVKITGLEMDSRKITNGNLFICVSGIDGFLEDRHQFVEDAV
CCHHHHHHHHHHHHHHCCCEEEEEEEEECCCEECCCCEEEEEECCCHHHHHHHHHHHHHH
KNGAVALIVERDVNIEIPKIIVKDARYAMAVIASHFYGYPSNKMKLIGITGTNGKTTTSY
CCCCEEEEEECCCCEECCHHEEHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHH
LIEKILSDYGYHTGLMGNNGVKVGSKWYSTDINTQEPPTLQRNLQMMKNQNADYCVMEVT
HHHHHHHHCCCCCCCCCCCCEEECCEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEEC
SQGLHMERVKGCNFKIAVFTNLTQDHLDYHGTFDEYKHVKSLLFARLGNDLSTTDKKIAV
CCCCCHHHHCCCCEEEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEE
LNADDPSVEYFKKITSAEVITYGIHNGADVQAKNITLSPKGIRFLVSSFNGEIEVSLNLV
EECCCCCHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCHHHHHEECCCCEEEEEEEEE
GRFNVYNALAAITVALVEGIPLTNISDSLSTLKSIEGRMEIIDENQDFLVIVDYAHTPDA
EECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCHH
LENVLSTISEFSKGKVITVFGCGGDRDAKKRSIMGGIAGSYSDFVFITSDNPRSEDPQAI
HHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHH
TKDIEKGFSQNNNLNYKVEVDRELAINHAINMASSNDIVLIAGKGHETYQILKDSTIHFD
HHHHHHHCCCCCCCEEEEEECHHEEEHHHEEECCCCCEEEEECCCCCEEEEECCCEEECC
DKEKARQAIINNR
CHHHHHHHHCCCC
>Mature Secondary Structure
MKLKELANLLLVKETIGDMNVKITGLEMDSRKITNGNLFICVSGIDGFLEDRHQFVEDAV
CCHHHHHHHHHHHHHHCCCEEEEEEEEECCCEECCCCEEEEEECCCHHHHHHHHHHHHHH
KNGAVALIVERDVNIEIPKIIVKDARYAMAVIASHFYGYPSNKMKLIGITGTNGKTTTSY
CCCCEEEEEECCCCEECCHHEEHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHH
LIEKILSDYGYHTGLMGNNGVKVGSKWYSTDINTQEPPTLQRNLQMMKNQNADYCVMEVT
HHHHHHHHCCCCCCCCCCCCEEECCEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEEC
SQGLHMERVKGCNFKIAVFTNLTQDHLDYHGTFDEYKHVKSLLFARLGNDLSTTDKKIAV
CCCCCHHHHCCCCEEEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEE
LNADDPSVEYFKKITSAEVITYGIHNGADVQAKNITLSPKGIRFLVSSFNGEIEVSLNLV
EECCCCCHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCHHHHHEECCCCEEEEEEEEE
GRFNVYNALAAITVALVEGIPLTNISDSLSTLKSIEGRMEIIDENQDFLVIVDYAHTPDA
EECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCHH
LENVLSTISEFSKGKVITVFGCGGDRDAKKRSIMGGIAGSYSDFVFITSDNPRSEDPQAI
HHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHH
TKDIEKGFSQNNNLNYKVEVDRELAINHAINMASSNDIVLIAGKGHETYQILKDSTIHFD
HHHHHHHCCCCCCCEEEEEECHHEEEHHHEEECCCCCEEEEECCCCCEEEEECCCEEECC
DKEKARQAIINNR
CHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436954; 9384377; 8289242 [H]