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The map label for this gene is dacA [H]

Identifier: 218903688

GI number: 218903688

Start: 2446336

End: 2447352

Strand: Reverse

Name: dacA [H]

Synonym: BCAH820_2572

Alternate gene names: 218903688

Gene position: 2447352-2446336 (Counterclockwise)

Preceding gene: 218903689

Following gene: 218903674

Centisome position: 46.15

GC content: 30.88

Gene sequence:

>1017_bases
ATGCAATTCTTAAAAAAAATCACAATTTTATTATTATCTTTTGTTATTACCGCTCTTATCGTTTTATATTTTTATCTTTA
TGTAAATGGTCCCATTATTCAAGCTAAATCAGCTATTTTAATAGATGCCAATTCAGGTGAAGTTGTTTATAAAAAAAATG
AAGAAAATTCTATACAATCGGCTACTTTATCTAAATTAATGACAGAATATATCGTACTAGAACAACTGGATAAAGGAAAT
ATACAGTTAGATGAAGTAGTAAAAATAAGTAACGAAGTTTTCCGAGCAGAAACGAGTCCTATACAAGTAACTTCGAAAGA
TAAAACAACGGTTCGTGATTTACTTCATGCATTGTTATTGACAGGGAATAATCGTTCCACACTTGCCCTTGCAGAACACA
TTGCTGGAAACGAAGATAATTTCACTCAGCTAATGAATGAAAAAGCAAAACAACTAAAGTTATCACAACCATCTCCGTTT
CTAAACGCTACTGGAATGAATAACGGTACAAATAAACTTTCAACTACAACAGCAATTGAAGTTGGTAAACTAGCAACAAG
ATTAGTAACAGACTTTCCAGATGTACTGAACGTTACAAAACTAACCTCTTACCAGTTCACCTTCAAAGATACCAAAGTTT
TCAATACCAATAAAATGATTTATTCCCTAAATAAAAACATTAAACTCCAAGGCGTTGATGGCTTACAAACTAGTTTTTCA
ACTAATAGTAATTATAGTTTTGTCAGTACAGCAAAACGAGGAGATACTAGACTTATTTCAGTAATATTAGACGCCGATAA
TGAAAACAGTACTTTTATTGAAACAAAAAAATTACTACAGTACGGCTTTAATCCCTCCTCATACTCTGCACTTCAAGCAT
TTAAAGATGCTGTTACCTCTTGGGCAATTTTGCTTCAGTTTAAAAATTTGATCATACAAACATTGTTGATTTTCTTTATT
ATTACAATTTTAATGTTTTTACATATACGTCAGAAAAAATCCGAGGATTTCAACTAG

Upstream 100 bases:

>100_bases
TCCTTTATAATTCTTTAGAAATTCTTTACCTTTTCTTTCTTCCTAATTAAGTATTCCTTTTTACAATATTATTATCGACG
TCATAATAGGAGGATCGCTT

Downstream 100 bases:

>100_bases
GAAATCTTCTTATATATAAAGAACGGCTTGCATTTACAGTGCAAGCCGTTTTTTAAAACTATAGTCCTCTTCCAATTAAA
TTCGCTGAAACAGGCCGAAG

Product: D-alanyl-D-alanine carboxypeptidase family protein

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MQFLKKITILLLSFVITALIVLYFYLYVNGPIIQAKSAILIDANSGEVVYKKNEENSIQSATLSKLMTEYIVLEQLDKGN
IQLDEVVKISNEVFRAETSPIQVTSKDKTTVRDLLHALLLTGNNRSTLALAEHIAGNEDNFTQLMNEKAKQLKLSQPSPF
LNATGMNNGTNKLSTTTAIEVGKLATRLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKNIKLQGVDGLQTSFS
TNSNYSFVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFNPSSYSALQAFKDAVTSWAILLQFKNLIIQTLLIFFI
ITILMFLHIRQKKSEDFN

Sequences:

>Translated_338_residues
MQFLKKITILLLSFVITALIVLYFYLYVNGPIIQAKSAILIDANSGEVVYKKNEENSIQSATLSKLMTEYIVLEQLDKGN
IQLDEVVKISNEVFRAETSPIQVTSKDKTTVRDLLHALLLTGNNRSTLALAEHIAGNEDNFTQLMNEKAKQLKLSQPSPF
LNATGMNNGTNKLSTTTAIEVGKLATRLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKNIKLQGVDGLQTSFS
TNSNYSFVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFNPSSYSALQAFKDAVTSWAILLQFKNLIIQTLLIFFI
ITILMFLHIRQKKSEDFN
>Mature_338_residues
MQFLKKITILLLSFVITALIVLYFYLYVNGPIIQAKSAILIDANSGEVVYKKNEENSIQSATLSKLMTEYIVLEQLDKGN
IQLDEVVKISNEVFRAETSPIQVTSKDKTTVRDLLHALLLTGNNRSTLALAEHIAGNEDNFTQLMNEKAKQLKLSQPSPF
LNATGMNNGTNKLSTTTAIEVGKLATRLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKNIKLQGVDGLQTSFS
TNSNYSFVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFNPSSYSALQAFKDAVTSWAILLQFKNLIIQTLLIFFI
ITILMFLHIRQKKSEDFN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=262, Percent_Identity=30.9160305343511, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI87082022, Length=259, Percent_Identity=27.7992277992278, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1786851, Length=266, Percent_Identity=28.9473684210526, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87082054, Length=259, Percent_Identity=25.8687258687259, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 37988; Mature: 37988

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFLKKITILLLSFVITALIVLYFYLYVNGPIIQAKSAILIDANSGEVVYKKNEENSIQS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCEEEECCCCCHHHH
ATLSKLMTEYIVLEQLDKGNIQLDEVVKISNEVFRAETSPIQVTSKDKTTVRDLLHALLL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHH
TGNNRSTLALAEHIAGNEDNFTQLMNEKAKQLKLSQPSPFLNATGMNNGTNKLSTTTAIE
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
VGKLATRLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKNIKLQGVDGLQTSFS
HHHHHHHHHHCCHHHHHHHHHEEEEEEECCCEEEECCCEEEEECCCEEEEECCCCHHCCC
TNSNYSFVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFNPSSYSALQAFKDAVTS
CCCCCEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
WAILLQFKNLIIQTLLIFFIITILMFLHIRQKKSEDFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQFLKKITILLLSFVITALIVLYFYLYVNGPIIQAKSAILIDANSGEVVYKKNEENSIQS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCEEEECCCCCHHHH
ATLSKLMTEYIVLEQLDKGNIQLDEVVKISNEVFRAETSPIQVTSKDKTTVRDLLHALLL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHH
TGNNRSTLALAEHIAGNEDNFTQLMNEKAKQLKLSQPSPFLNATGMNNGTNKLSTTTAIE
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
VGKLATRLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKNIKLQGVDGLQTSFS
HHHHHHHHHHCCHHHHHHHHHEEEEEEECCCEEEECCCEEEEECCCEEEEECCCCHHCCC
TNSNYSFVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFNPSSYSALQAFKDAVTS
CCCCCEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
WAILLQFKNLIIQTLLIFFIITILMFLHIRQKKSEDFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]