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The map label for this gene is yrkH [H]

Identifier: 218903668

GI number: 218903668

Start: 2428624

End: 2430060

Strand: Direct

Name: yrkH [H]

Synonym: BCAH820_2552

Alternate gene names: 218903668

Gene position: 2428624-2430060 (Clockwise)

Preceding gene: 218903667

Following gene: 218903669

Centisome position: 45.8

GC content: 34.79

Gene sequence:

>1437_bases
ATGCTTTTAAAATATTTTTATGATGAAAAATTAGCACATGCTTCCTATTTAGTCGGTTGTCAGAAGGAAGGTGTAGCAAT
TGTAATTGATCCAGGTCGCTATATAGAACAATATATAGAATTTGCTAAGAAAGAGGGGATGGAAGTAATTGCTGCGGCTG
AAACTCACATTCATGCAGATTTTCTTTCTGGATCTAGAGAACTTTCTACTCTTTATCATGCCAATTTATATGTATCTGAT
GTAGGTGATTGTAATTGGAAATATCAATATCTTAACGAAGGTCAATACAATTTGGTTAGAGAGGGCACAGAGTTTAAAGT
AGGTCATATAAAATTTAATGTAATTCACACACCAGGGCATACACCTGAAAGTATTTCTTTTTTAGTAACTGATACAAGTC
AAAATAACTATACGAATGATAAGCCAATAGGAATATTCACAGGTGATTTTATATTTGTTGGAGATATAGGAAGACCCGAT
TTATTAGAAACTGCTGTAGGTATGAAAGATACCGCAAAAATAGGAGCAAAACAATTATTTGATTCGATACAAAAAATAAA
AACACTCCCTGATTATTTGCAAATATGGCCATCACATGGTGCAGGAAGCGCATGTGGAAAAGCATTAGGAGCAATTCCAA
CTTCTACCTTAGGTTATGAAAAGATGTTCAATTGGGCATTTCAGTGTAATGAAGAAAGTGATTTTATATCGACTTTATTG
ACGGGGCAGCCAGAGCCTCCAAGGTATTTTTCTTTAATGAAGAATTTAAATAAGTATGGTCCGCCCATTCGTAAGAAAAG
AGAGATTTTTGCTATTAAAACAGTAGAGGAACTTCAAGGAGTTATGAGGAGCGTTCAGCAAATAGTTGATATAAGGGATG
TAGAGAGTTTTGCTTCTGGTCACATCGAGAAATCAATTAACATACCGTATAACAATTCCTTCACAACCTGGTGTGGATGG
TTACTAGATTATAAAACAGAAACTTTAATCATTCTAGATGAGGAAAAGGTTAGAGTGGAGGAGGTTATTAGAGACTTTGA
ATCTATTGGTCTAGATAATATTATTGCCTTTGCACCCTTACAAGTAATACAAAGACTCGATAGATTTGAAAGTTACAAAG
AAAAAACATCGATTGAATTATATCCGCATATAAAGGATGGTAGGGTTAAGGTAATTGATGTACGTAGTAAAAAAGAGTGG
GACGAAGGACATCTTCATGATGCAATACATATCCCTTTAGGAAATCTATTGAAGCAGCTAGATTGTATACCAAAGGATTG
CCCAATCGTTTTACAATGTCGAACCGGATTACGTTCCGCTATAGCAGCTAGTATTTTACAGAGAGCTGGTATAAAGGAAG
TAGTTAATCTAAAGGGAGGATTTCTTGCATGGAATAAAGAAGAGCTTCCTTATACAACTTGTAATCTCAATGTGTAG

Upstream 100 bases:

>100_bases
GTATTGGAACTTTATGTTGAAAGGGATCCTATAGGAGAATATAGAAAGCGGTTACAAATGTTGAAAAGTAATTTGAAATA
ACTATTTGGAGGGTATAGCT

Downstream 100 bases:

>100_bases
GGAAATAATTTTTATTAATTTATCTGATAAGGAAAATTACGGATTACATAGGAGTATATCTATAAATAGGAGAAATAGAT
AATTTATTCTATAGATCTAA

Product: metallo-beta-lactamase/rhodanese-like domain protein

Products: glutathione; 2-hydroxy carboxylate

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MLLKYFYDEKLAHASYLVGCQKEGVAIVIDPGRYIEQYIEFAKKEGMEVIAAAETHIHADFLSGSRELSTLYHANLYVSD
VGDCNWKYQYLNEGQYNLVREGTEFKVGHIKFNVIHTPGHTPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGDIGRPD
LLETAVGMKDTAKIGAKQLFDSIQKIKTLPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFQCNEESDFISTLL
TGQPEPPRYFSLMKNLNKYGPPIRKKREIFAIKTVEELQGVMRSVQQIVDIRDVESFASGHIEKSINIPYNNSFTTWCGW
LLDYKTETLIILDEEKVRVEEVIRDFESIGLDNIIAFAPLQVIQRLDRFESYKEKTSIELYPHIKDGRVKVIDVRSKKEW
DEGHLHDAIHIPLGNLLKQLDCIPKDCPIVLQCRTGLRSAIAASILQRAGIKEVVNLKGGFLAWNKEELPYTTCNLNV

Sequences:

>Translated_478_residues
MLLKYFYDEKLAHASYLVGCQKEGVAIVIDPGRYIEQYIEFAKKEGMEVIAAAETHIHADFLSGSRELSTLYHANLYVSD
VGDCNWKYQYLNEGQYNLVREGTEFKVGHIKFNVIHTPGHTPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGDIGRPD
LLETAVGMKDTAKIGAKQLFDSIQKIKTLPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFQCNEESDFISTLL
TGQPEPPRYFSLMKNLNKYGPPIRKKREIFAIKTVEELQGVMRSVQQIVDIRDVESFASGHIEKSINIPYNNSFTTWCGW
LLDYKTETLIILDEEKVRVEEVIRDFESIGLDNIIAFAPLQVIQRLDRFESYKEKTSIELYPHIKDGRVKVIDVRSKKEW
DEGHLHDAIHIPLGNLLKQLDCIPKDCPIVLQCRTGLRSAIAASILQRAGIKEVVNLKGGFLAWNKEELPYTTCNLNV
>Mature_478_residues
MLLKYFYDEKLAHASYLVGCQKEGVAIVIDPGRYIEQYIEFAKKEGMEVIAAAETHIHADFLSGSRELSTLYHANLYVSD
VGDCNWKYQYLNEGQYNLVREGTEFKVGHIKFNVIHTPGHTPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGDIGRPD
LLETAVGMKDTAKIGAKQLFDSIQKIKTLPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFQCNEESDFISTLL
TGQPEPPRYFSLMKNLNKYGPPIRKKREIFAIKTVEELQGVMRSVQQIVDIRDVESFASGHIEKSINIPYNNSFTTWCGW
LLDYKTETLIILDEEKVRVEEVIRDFESIGLDNIIAFAPLQVIQRLDRFESYKEKTSIELYPHIKDGRVKVIDVRSKKEW
DEGHLHDAIHIPLGNLLKQLDCIPKDCPIVLQCRTGLRSAIAASILQRAGIKEVVNLKGGFLAWNKEELPYTTCNLNV

Specific function: Unknown

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI41327741, Length=202, Percent_Identity=26.7326732673267, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI1787158, Length=225, Percent_Identity=26.2222222222222, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538952, Length=187, Percent_Identity=25.668449197861, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR001763 [H]

Pfam domain/function: PF00753 Lactamase_B; PF00581 Rhodanese [H]

EC number: 3.1.2.6

Molecular weight: Translated: 54144; Mature: 54144

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKYFYDEKLAHASYLVGCQKEGVAIVIDPGRYIEQYIEFAKKEGMEVIAAAETHIHAD
CEEHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEHHHHHHHH
FLSGSRELSTLYHANLYVSDVGDCNWKYQYLNEGQYNLVREGTEFKVGHIKFNVIHTPGH
HHCCCHHHHHHEECEEEEECCCCCCEEEEEECCCCEEEEECCCCEEEEEEEEEEEECCCC
TPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGDIGRPDLLETAVGMKDTAKIGAKQLF
CCCCEEEEEEECCCCCCCCCCCEEEEECCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHH
DSIQKIKTLPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFQCNEESDFISTLL
HHHHHHHCCCHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHEECCCCHHHHHHHH
TGQPEPPRYFSLMKNLNKYGPPIRKKREIFAIKTVEELQGVMRSVQQIVDIRDVESFASG
CCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HIEKSINIPYNNSFTTWCGWLLDYKTETLIILDEEKVRVEEVIRDFESIGLDNIIAFAPL
CCCEEECCCCCCCHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHH
QVIQRLDRFESYKEKTSIELYPHIKDGRVKVIDVRSKKEWDEGHLHDAIHIPLGNLLKQL
HHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCEEECCHHHHHHHH
DCIPKDCPIVLQCRTGLRSAIAASILQRAGIKEVVNLKGGFLAWNKEELPYTTCNLNV
HCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECCCCCCEEEEECCC
>Mature Secondary Structure
MLLKYFYDEKLAHASYLVGCQKEGVAIVIDPGRYIEQYIEFAKKEGMEVIAAAETHIHAD
CEEHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEHHHHHHHH
FLSGSRELSTLYHANLYVSDVGDCNWKYQYLNEGQYNLVREGTEFKVGHIKFNVIHTPGH
HHCCCHHHHHHEECEEEEECCCCCCEEEEEECCCCEEEEECCCCEEEEEEEEEEEECCCC
TPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGDIGRPDLLETAVGMKDTAKIGAKQLF
CCCCEEEEEEECCCCCCCCCCCEEEEECCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHH
DSIQKIKTLPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFQCNEESDFISTLL
HHHHHHHCCCHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHEECCCCHHHHHHHH
TGQPEPPRYFSLMKNLNKYGPPIRKKREIFAIKTVEELQGVMRSVQQIVDIRDVESFASG
CCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HIEKSINIPYNNSFTTWCGWLLDYKTETLIILDEEKVRVEEVIRDFESIGLDNIIAFAPL
CCCEEECCCCCCCHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHH
QVIQRLDRFESYKEKTSIELYPHIKDGRVKVIDVRSKKEWDEGHLHDAIHIPLGNLLKQL
HHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCEEECCHHHHHHHH
DCIPKDCPIVLQCRTGLRSAIAASILQRAGIKEVVNLKGGFLAWNKEELPYTTCNLNV
HCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-(2-hydroxyacyl)glutathione; H2O

Specific reaction: S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]