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The map label for this gene is spoT [H]

Identifier: 218903585

GI number: 218903585

Start: 2347225

End: 2348613

Strand: Reverse

Name: spoT [H]

Synonym: BCAH820_2469

Alternate gene names: 218903585

Gene position: 2348613-2347225 (Counterclockwise)

Preceding gene: 218903589

Following gene: 218903584

Centisome position: 44.29

GC content: 28.65

Gene sequence:

>1389_bases
ATGCGCGAGGTATTATTACAAAAGGAAAATTATTTACAATCTAATGAATTAAAACTTTTGAAAAAAGCTATACAATTTGC
AGAGCGAGCACATGATGGACAATACCGCCAAACCGGAGAACCATATATTATTCACCCTTTCGCAATTGCTGAAATACTGA
TAAATTATAAAGCTGACATCACTACATTGATTGCTGCATTATTACACGATGTTGTAGAAGATACTGAACATTCTATAGAA
GAAATTGAATCGCATTTTGGTACAACTATTAGATATATTGTAAATGGATTAACAAAAAGAAAAAAACAACAAAATCAAAA
CAAAATAATATACGAAGCAATTAATTTTAAAAAACTACTATTATCCTCTCAACATGATATACGTGTTGGCATTATTAAAG
TTATAGATCGCCTTCACAATATAAAAACATTGAATGTTAAAAAACCGGCGAAACAAGTAGCATATGCGAATGAAACTTTA
ACACTTTTTGCGCCACTTGCAAAGCGACTAGGATTATATACAATCCAGTATGAATTAGAAAATCTTGCTTTTCAATATTT
ACATAAAGAAAGATATAACAAGGTACTTGAATTTTTAACTGGATATGTGCCACATCTCCAAAAAAATATAACTGATTTGA
GAAGAGGAATTCATTCTTTTTATGATACTAACCTTTCATTTAAAATAAGCTATGAGTTTCCTCCAATTTATACTGCTTAC
TCACAACTACAAGAAGTACAAAACGTATCAGGTATTTCACAAATCTGTATTACAACTACAAGTGTACTAGATTGCTATAA
AATTCTTGGAATAATACATCAATTATACAAACCAACTATCAACTATTTCGAAGACAACATAGCACTTGAAAACAGCCCTT
TTAATAGCAATTTAAAAACTAAAATCAATATGAATAATACTGTACAAACAATTATCATTCAAACACAATTAGCAAAAACA
TTAAAAGATAACGGCATTTTTTCTTTATTGAATAATAGAACTGCAAATGTTCAAGCAATATCTTTTAAAATAATGAATGA
TACAATTGTGAATAACAATTTATTAACTACAGATCCAATCGCTTTCCATAATCTAGTATCATATGAACTATTTGAAAATA
CCATAACGGCTTATACTTCAGATTTACAACCTATTTATTTACCAGATGGTGCTACAATTATTGATTTTACTTTCTCTTCA
TTTCCAAAAATTGCACATAACATGAAAAATGCAAAAGTAAATGGTATACCTGTTCCACTTAAAACAAAGGTATCTCATTT
TGACGTCATAGAAATCTATTTTGGTTTAAAACAAAACTTGGTATTAGAGTGGCTCAATTATGCCAATACCGCTAAAGCAC
AATTAATTATTCAGCAAAAACTATCTTAA

Upstream 100 bases:

>100_bases
CTCAACACCTTCATTTATTTCATAATTAAAATAATTCCAAAGGGTACTTATTCTATTATACAGAATTATTATTAAAAATA
ATTTTAGGGGATGGATTTAT

Downstream 100 bases:

>100_bases
ATAACAGACTAATACCTCTTATAAAAAGTATTGGTCTGTTATTTAGGATACGAAATACACCCCCTATTCTTTATGGAATA
GGAAAATTTATTATGATACC

Product: GTP pyrophosphokinase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MREVLLQKENYLQSNELKLLKKAIQFAERAHDGQYRQTGEPYIIHPFAIAEILINYKADITTLIAALLHDVVEDTEHSIE
EIESHFGTTIRYIVNGLTKRKKQQNQNKIIYEAINFKKLLLSSQHDIRVGIIKVIDRLHNIKTLNVKKPAKQVAYANETL
TLFAPLAKRLGLYTIQYELENLAFQYLHKERYNKVLEFLTGYVPHLQKNITDLRRGIHSFYDTNLSFKISYEFPPIYTAY
SQLQEVQNVSGISQICITTTSVLDCYKILGIIHQLYKPTINYFEDNIALENSPFNSNLKTKINMNNTVQTIIIQTQLAKT
LKDNGIFSLLNNRTANVQAISFKIMNDTIVNNNLLTTDPIAFHNLVSYELFENTITAYTSDLQPIYLPDGATIIDFTFSS
FPKIAHNMKNAKVNGIPVPLKTKVSHFDVIEIYFGLKQNLVLEWLNYANTAKAQLIIQQKLS

Sequences:

>Translated_462_residues
MREVLLQKENYLQSNELKLLKKAIQFAERAHDGQYRQTGEPYIIHPFAIAEILINYKADITTLIAALLHDVVEDTEHSIE
EIESHFGTTIRYIVNGLTKRKKQQNQNKIIYEAINFKKLLLSSQHDIRVGIIKVIDRLHNIKTLNVKKPAKQVAYANETL
TLFAPLAKRLGLYTIQYELENLAFQYLHKERYNKVLEFLTGYVPHLQKNITDLRRGIHSFYDTNLSFKISYEFPPIYTAY
SQLQEVQNVSGISQICITTTSVLDCYKILGIIHQLYKPTINYFEDNIALENSPFNSNLKTKINMNNTVQTIIIQTQLAKT
LKDNGIFSLLNNRTANVQAISFKIMNDTIVNNNLLTTDPIAFHNLVSYELFENTITAYTSDLQPIYLPDGATIIDFTFSS
FPKIAHNMKNAKVNGIPVPLKTKVSHFDVIEIYFGLKQNLVLEWLNYANTAKAQLIIQQKLS
>Mature_462_residues
MREVLLQKENYLQSNELKLLKKAIQFAERAHDGQYRQTGEPYIIHPFAIAEILINYKADITTLIAALLHDVVEDTEHSIE
EIESHFGTTIRYIVNGLTKRKKQQNQNKIIYEAINFKKLLLSSQHDIRVGIIKVIDRLHNIKTLNVKKPAKQVAYANETL
TLFAPLAKRLGLYTIQYELENLAFQYLHKERYNKVLEFLTGYVPHLQKNITDLRRGIHSFYDTNLSFKISYEFPPIYTAY
SQLQEVQNVSGISQICITTTSVLDCYKILGIIHQLYKPTINYFEDNIALENSPFNSNLKTKINMNNTVQTIIIQTQLAKT
LKDNGIFSLLNNRTANVQAISFKIMNDTIVNNNLLTTDPIAFHNLVSYELFENTITAYTSDLQPIYLPDGATIIDFTFSS
FPKIAHNMKNAKVNGIPVPLKTKVSHFDVIEIYFGLKQNLVLEWLNYANTAKAQLIIQQKLS

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance [H]

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=145, Percent_Identity=34.4827586206897, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1790082, Length=463, Percent_Identity=30.0215982721382, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1789147, Length=441, Percent_Identity=22.6757369614512, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25143118, Length=134, Percent_Identity=35.0746268656716, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 53095; Mature: 53095

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREVLLQKENYLQSNELKLLKKAIQFAERAHDGQYRQTGEPYIIHPFAIAEILINYKADI
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHCCCHH
TTLIAALLHDVVEDTEHSIEEIESHFGTTIRYIVNGLTKRKKQQNQNKIIYEAINFKKLL
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHH
LSSQHDIRVGIIKVIDRLHNIKTLNVKKPAKQVAYANETLTLFAPLAKRLGLYTIQYELE
HCCCCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHCCCHHHHHHHHHHHHCEEEEEEHHH
NLAFQYLHKERYNKVLEFLTGYVPHLQKNITDLRRGIHSFYDTNLSFKISYEFPPIYTAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
SQLQEVQNVSGISQICITTTSVLDCYKILGIIHQLYKPTINYFEDNIALENSPFNSNLKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCEE
KINMNNTVQTIIIQTQLAKTLKDNGIFSLLNNRTANVQAISFKIMNDTIVNNNLLTTDPI
EEECCCCEEEEEEHHHHHHHHHCCCCEEHHCCCCCCEEEEEEEEECCEEECCCCCCCCCH
AFHNLVSYELFENTITAYTSDLQPIYLPDGATIIDFTFSSFPKIAHNMKNAKVNGIPVPL
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECHHHHHHHHHCCCCCCCCCCCCCC
KTKVSHFDVIEIYFGLKQNLVLEWLNYANTAKAQLIIQQKLS
CCCCCCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MREVLLQKENYLQSNELKLLKKAIQFAERAHDGQYRQTGEPYIIHPFAIAEILINYKADI
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHCCCHH
TTLIAALLHDVVEDTEHSIEEIESHFGTTIRYIVNGLTKRKKQQNQNKIIYEAINFKKLL
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHH
LSSQHDIRVGIIKVIDRLHNIKTLNVKKPAKQVAYANETLTLFAPLAKRLGLYTIQYELE
HCCCCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHCCCHHHHHHHHHHHHCEEEEEEHHH
NLAFQYLHKERYNKVLEFLTGYVPHLQKNITDLRRGIHSFYDTNLSFKISYEFPPIYTAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
SQLQEVQNVSGISQICITTTSVLDCYKILGIIHQLYKPTINYFEDNIALENSPFNSNLKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCEE
KINMNNTVQTIIIQTQLAKTLKDNGIFSLLNNRTANVQAISFKIMNDTIVNNNLLTTDPI
EEECCCCEEEEEEHHHHHHHHHCCCCEEHHCCCCCCEEEEEEEEECCEEECCCCCCCCCH
AFHNLVSYELFENTITAYTSDLQPIYLPDGATIIDFTFSSFPKIAHNMKNAKVNGIPVPL
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECHHHHHHHHHCCCCCCCCCCCCCC
KTKVSHFDVIEIYFGLKQNLVLEWLNYANTAKAQLIIQQKLS
CCCCCCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]