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The map label for this gene is pncA [H]

Identifier: 218903401

GI number: 218903401

Start: 2170459

End: 2171007

Strand: Direct

Name: pncA [H]

Synonym: BCAH820_2285

Alternate gene names: 218903401

Gene position: 2170459-2171007 (Clockwise)

Preceding gene: 218903400

Following gene: 218903403

Centisome position: 40.93

GC content: 33.88

Gene sequence:

>549_bases
ATGAAACGAGCTCTTATAAATATTGATTATACATATGATTTTGTAGCGGAAAAAGGTGCTTTAACTTGCGGAAAACCCGG
TCAAGAAATTGAGAAAGAACTTGTACATATAACGAAGCGATATATCGAAAATGGAGATTACGTTGTCTTTGCTATTGATA
AACATGAAGAGAATGATGTATATCATCCTGAATCAAAATTATTCCCGCCTCATAATATAGCGGGTACAAATGGAAGAGAT
TTGTTTGGTGAATTACAAGATGTATATGAAACATATAAAGATGATGAGAATGTATATTACATGGATAAAACACGGTATAG
TGCATTTGCAGGAACAGACTTAGAGATGAAGTTAAGAGAAAGAGGAATTGAAGAGGTTCACCTTGTAGGGGTATGTACTG
ATATTTGTGTTCTTCATACAGCGGTAGATGCTTATAATAAAGGGTTTAAAATTGTAGTTTATGAAAAGGCGGTTGCATCT
TTTAATGCGCAAGGGCATGAATTTGCACTTGGACATTTTAAATCTTGTTTAAACGCAGAAGTGAAGTAA

Upstream 100 bases:

>100_bases
AAAATGTAGGTATTTCATTTGAAATGTCGAATAGTACATACGATGTCGTAATAACAAATTTTCTATTGTTTACTGTGAAA
CAGGAGAAGGAGAGATAGTA

Downstream 100 bases:

>100_bases
AAAGAGAGGCCTTTAAAAAAGGTCTCTTTTTACGTTTAAATTGAGACGAGGTTTTGCTCAATTAATTTTTTTATAAATGG
GCTAATATTCTCTGGTAGCT

Product: isochorismatase family protein

Products: nicotinate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MKRALINIDYTYDFVAEKGALTCGKPGQEIEKELVHITKRYIENGDYVVFAIDKHEENDVYHPESKLFPPHNIAGTNGRD
LFGELQDVYETYKDDENVYYMDKTRYSAFAGTDLEMKLRERGIEEVHLVGVCTDICVLHTAVDAYNKGFKIVVYEKAVAS
FNAQGHEFALGHFKSCLNAEVK

Sequences:

>Translated_182_residues
MKRALINIDYTYDFVAEKGALTCGKPGQEIEKELVHITKRYIENGDYVVFAIDKHEENDVYHPESKLFPPHNIAGTNGRD
LFGELQDVYETYKDDENVYYMDKTRYSAFAGTDLEMKLRERGIEEVHLVGVCTDICVLHTAVDAYNKGFKIVVYEKAVAS
FNAQGHEFALGHFKSCLNAEVK
>Mature_182_residues
MKRALINIDYTYDFVAEKGALTCGKPGQEIEKELVHITKRYIENGDYVVFAIDKHEENDVYHPESKLFPPHNIAGTNGRD
LFGELQDVYETYKDDENVYYMDKTRYSAFAGTDLEMKLRERGIEEVHLVGVCTDICVLHTAVDAYNKGFKIVVYEKAVAS
FNAQGHEFALGHFKSCLNAEVK

Specific function: Unknown

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

Organism=Escherichia coli, GI87081992, Length=71, Percent_Identity=40.8450704225352, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.5.1.19

Molecular weight: Translated: 20755; Mature: 20755

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRALINIDYTYDFVAEKGALTCGKPGQEIEKELVHITKRYIENGDYVVFAIDKHEENDV
CCCEEEEECCEEEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
YHPESKLFPPHNIAGTNGRDLFGELQDVYETYKDDENVYYMDKTRYSAFAGTDLEMKLRE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCCHHHHH
RGIEEVHLVGVCTDICVLHTAVDAYNKGFKIVVYEKAVASFNAQGHEFALGHFKSCLNAE
CCCCEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHCCCCCCEEHHHHHHHHHCCC
VK
CC
>Mature Secondary Structure
MKRALINIDYTYDFVAEKGALTCGKPGQEIEKELVHITKRYIENGDYVVFAIDKHEENDV
CCCEEEEECCEEEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
YHPESKLFPPHNIAGTNGRDLFGELQDVYETYKDDENVYYMDKTRYSAFAGTDLEMKLRE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCCHHHHH
RGIEEVHLVGVCTDICVLHTAVDAYNKGFKIVVYEKAVASFNAQGHEFALGHFKSCLNAE
CCCCEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHCCCCCCEEHHHHHHHHHCCC
VK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nicotinamide; H2O

Specific reaction: nicotinamide + H2O = nicotinate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]