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The map label for this gene is narH [H]

Identifier: 218903275

GI number: 218903275

Start: 2045724

End: 2047085

Strand: Direct

Name: narH [H]

Synonym: BCAH820_2159

Alternate gene names: 218903275

Gene position: 2045724-2047085 (Clockwise)

Preceding gene: 218903274

Following gene: 218903276

Centisome position: 38.58

GC content: 38.91

Gene sequence:

>1362_bases
ATGTATTTCAATAACGTAGAAACAAAACCTGGTATTGGTTATCCGAAGCAGTGGGAGGATCAGGAGAAATATAAAGGCGG
CTGGGAATTGAAAAATGGTGAAATTCAGCTGAAATCCGGTTCGAAAATGAAGCGCCTTATGAATATTTTCCACAATCCAG
ATCAACCAACAATTGATGATTACTTTGAACCGTGGAACTATGATTATGAAACGTTAACAAATAGTCCGCAGCGTAAGCAT
CAGCCAGTTGCTCGTCCAAAATCAGCAATTACAGGCGAATTTATCGACAAAATTGAATGGGGTCCAAACTGGGAAGATGA
TTTAGCTGGTGGACATATAACAGGATTACAAGATCCGAACGTAAAGAAAATGGAAGAAGAAATTAAAACCGATTTTGAAA
ATGTCTTTATGATGTATTTACCACGCATATGCGAACATTGTATGAATCCATCTTGCGTATCGTCTTGTCCATCTGGTGCG
ATGTATAAACGGGAAGAAGATGGGATTGTTCTTGTGGATCAAAACGCATGTCGTGCATGGAGATTCTGCGTATCGTCTTG
CCCATATAAAAAAGTGTACTTTAATTGGCAAACGAATAAAGCGGAAAAATGTACAATGTGTTTCCCACGTATTGAAGCTG
GTATGCCAACAATTTGTTCGGAAACATGTGTAGGACGTATCAGATATATTGGGGTAATGCTATATGACGCTGACAAAGTA
AAAGAAGCGGCTTCTGTTGAGAATGAAAAAGATTTATATGAATCTCAGCTTACTGTTTTCCTAGATCCAAATGATCCTGA
AGTAGCAGCTGAAGCGAAAAAACAAGGCATTCCTGAAGAGTGGATTAAAGCGGCACAACAGTCACCAATCTATAAAATGA
TTATCGATTGGAAAATTGCTCTACCTCTTCATCCGGAGTATCGTACAATGCCAATGGTTTGGTATATCCCACCGCTTAGC
CCAATTATGAATATGGTGGAAGGGAAAGGTAGTAACTGGCAAGCAGAAGAGATTTTCCCTGCTATCGATAATATGCGTAT
TCCAATTCAATATTTAGCGAATCTACTCACTGCAGGAGATGAATCACACATTCGACTTACATTGAAAAAAATGGCTGTTA
TGCGAACGCATATGAGAGCACTGCAAATTAATAAAGAACCAAATGAAGCTGTATTAAAAGAACTTGGTTTAACGAAACAG
GATGTAGAAGATATGTATCGTCTTCTTGCCATTGCAAAATATAAAGATCGCTTCGTCATCCCGACATCTCATCGTGAACA
AGTGGCAGATTTATACAGCGAGCAAGGAAGCTGTGGTTTATCATTTGCAGGTGGTCCAGGTTCTTGTATGACAATTTCTT
AA

Upstream 100 bases:

>100_bases
ACAAGTCGGAATGGTAATGAACCTAGATAAATGCATCGGCTGCCATACTTGTAGCGTAACGTGCAAAAACACCTGGACAA
ATCGTCCAGGTGCTGAATAT

Downstream 100 bases:

>100_bases
ATAATGGGGGCGGAAAAATATGAAACAAAGTCTACAAACTGCTTTTTCTTGTTCTTCATTCCTTCTCTCTTACCCGGAAC
TTGGGTGGAGAGAAGCTTTA

Product: respiratory nitrate reductase, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDDYFEPWNYDYETLTNSPQRKH
QPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVKKMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGA
MYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYFNWQTNKAEKCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKV
KEAASVENEKDLYESQLTVFLDPNDPEVAAEAKKQGIPEEWIKAAQQSPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLS
PIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDESHIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQ
DVEDMYRLLAIAKYKDRFVIPTSHREQVADLYSEQGSCGLSFAGGPGSCMTIS

Sequences:

>Translated_453_residues
MYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDDYFEPWNYDYETLTNSPQRKH
QPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVKKMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGA
MYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYFNWQTNKAEKCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKV
KEAASVENEKDLYESQLTVFLDPNDPEVAAEAKKQGIPEEWIKAAQQSPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLS
PIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDESHIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQ
DVEDMYRLLAIAKYKDRFVIPTSHREQVADLYSEQGSCGLSFAGGPGSCMTIS
>Mature_453_residues
MYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDDYFEPWNYDYETLTNSPQRKH
QPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVKKMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGA
MYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYFNWQTNKAEKCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKV
KEAASVENEKDLYESQLTVFLDPNDPEVAAEAKKQGIPEEWIKAAQQSPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLS
PIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDESHIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQ
DVEDMYRLLAIAKYKDRFVIPTSHREQVADLYSEQGSCGLSFAGGPGSCMTIS

Specific function: This Is A Second Nitrate Reductase Enzyme Which Can Substitute For The Nra Enzyme And Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=445, Percent_Identity=58.6516853932584, Blast_Score=580, Evalue=1e-167,
Organism=Escherichia coli, GI1787478, Length=448, Percent_Identity=57.8125, Blast_Score=566, Evalue=1e-162,
Organism=Escherichia coli, GI1787872, Length=132, Percent_Identity=35.6060606060606, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787122, Length=132, Percent_Identity=35.6060606060606, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=37.2093023255814, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI2367345, Length=141, Percent_Identity=26.9503546099291, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1789370, Length=102, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=32.183908045977, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=31.0344827586207, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 51986; Mature: 51986

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDD
CCCCCCCCCCCCCCCCCCCCHHHHCCCCEECCCEEEECCCHHHHHHHHHHCCCCCCCHHH
YFEPWNYDYETLTNSPQRKHQPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPN
HCCCCCCCHHHHCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCC
VKKMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEECCCCEEEECCCHHHHH
RFCVSSCPYKKVYFNWQTNKAEKCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKV
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEECHHHH
KEAASVENEKDLYESQLTVFLDPNDPEVAAEAKKQGIPEEWIKAAQQSPIYKMIIDWKIA
HHHHHCCCHHHHHHCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEE
LPLHPEYRTMPMVWYIPPLSPIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGD
ECCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCC
ESHIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQDVEDMYRLLAIAKYKDRFVI
CCCEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEC
PTSHREQVADLYSEQGSCGLSFAGGPGSCMTIS
CCCHHHHHHHHHHCCCCCCEEECCCCCCEEEEC
>Mature Secondary Structure
MYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDD
CCCCCCCCCCCCCCCCCCCCHHHHCCCCEECCCEEEECCCHHHHHHHHHHCCCCCCCHHH
YFEPWNYDYETLTNSPQRKHQPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPN
HCCCCCCCHHHHCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCC
VKKMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEECCCCEEEECCCHHHHH
RFCVSSCPYKKVYFNWQTNKAEKCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKV
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEECHHHH
KEAASVENEKDLYESQLTVFLDPNDPEVAAEAKKQGIPEEWIKAAQQSPIYKMIIDWKIA
HHHHHCCCHHHHHHCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEE
LPLHPEYRTMPMVWYIPPLSPIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGD
ECCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCC
ESHIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQDVEDMYRLLAIAKYKDRFVI
CCCEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEC
PTSHREQVADLYSEQGSCGLSFAGGPGSCMTIS
CCCHHHHHHHHHHCCCCCCEEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]