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The map label for this gene is gatA [H]

Identifier: 218903219

GI number: 218903219

Start: 1997079

End: 1998554

Strand: Direct

Name: gatA [H]

Synonym: BCAH820_2103

Alternate gene names: 218903219

Gene position: 1997079-1998554 (Clockwise)

Preceding gene: 218903218

Following gene: 218903221

Centisome position: 37.66

GC content: 36.11

Gene sequence:

>1476_bases
ATGGAGATGCAGTTCAATACACTATTACAAAAAGAATTAACAATTCATGATATACAAACAGCGATGGAAGATGAAAAGTT
AACTTCAAAAGAATTAGTTATGTATTATCTTCATAGAATTGCAAAGTATGATCAAGACGGACCGAAAATTAATTCTATTT
TAGAAATAAATCCGGACGCTATTTTTATTGCAGAAGCGTTAGATCATGAAAGAAAAATTAAAGGTATAAGAGGTCCATTA
CATGGTATACCTGTTTTACTTAAAGACAATATTGAAACGAACGACTCTATGCATACAAGTGCAGGTACGATTGCTCTAGA
ACAACATATAAGTAGTGAAGATGCATTTCTCGTTACGAAGCTGAGAGAAGCAGGGGCAGTGATACTCGGGAAAACAAATA
TGACAGAATTAGCAAATGCAATGTCTTTTGAAATGTGGGCTGGATATAGTGCAAGAGGTGGGCAAACAATCAATCCTTAC
GGTACGGGTGACGATGATATGTTTGTTGGTGGCTCAAGTACAGGATCTGCGATAGCAGTTGCTGCTAACTTTACAGTAGT
ATCAGTTGGAACAGAAACGGATGCTTCTATTTTGAGTCCAGCTGTTCAAAACTCTGTAGTAGGTATAAAACCGACTGTTG
GTTTAATTAGTCGTAGAGGTATTATTCCGTTCACATATTCGCAAGATACAGCCGGTCCATTTGCTAGAACCGTAACAGAT
GCAGCAATCTTACTAGGGAGTTTAACTGGGGTAGATGAGAAAGATGTAGCTACTCATAAAAGTGAAGGGATAGGAGAACC
TGATTATACGAAATACCTTGATGTTAATGGGTTAAATGGAACAAAGATTGGTGTATATAACAACGCACCAAAAGAATATT
ATGAAACTGGTGAGTATGATGAAAAATTGTTTAAGGAAACGATTGAAGTATTGCATAGTAAGGGTGCGACAGTCGTAGAG
GATATTCATATTCCATCTTTTCATAGAGAATGGAGCTGGGGAGTATCGCTCTATGAACTAAAGCATAGTTTAGATAACTA
TCTTTCGAAATTACCTTCTACTATTCCAGTACATTCTATTTCAGAGTTAATGGAGTTTAATGAAAATATAGCAGAAAGGG
CTTTGAAATATGGACAAACTAAGTTAGAAAGAAGAAAAGATTTTCCTAATACGTTAAGGAATCCAGAGTATTTGAATGCA
AGATTAGAAGATATATATTTTTCTCAAGAACAAGGCATTGATTTTGCACTGGAAAAATATAATCTTGATGCGATTCTGTT
CCCTTCTTATATAGGCTCCACTATATGTGCAAAAGCAGGTTATCCGTCTATAGCAATACCTGCAGGGTATATGGAAGGTG
GAAGACCTTTTGGGATTACTCTTGCAAGTACAGCTTTTAGTGAAGGGACATTAATTAAACTAGCATACGCTTTTGAACAA
GCGACAAACCATAGAAAAATCCCGGGATTGTCATAA

Upstream 100 bases:

>100_bases
ATTCTTATAGAGGGATTGTAGTTGTATATGTGAGGAGCGGAGAAAATGAAAAAGTAATAGAGACATGCTATCAATAAAGG
GTTATAGAAGGAGAATGAAA

Downstream 100 bases:

>100_bases
GTAAAATATACAAAAAGGACAGGAATTCCTGTCCTTTTTGCTTTATTGATTACATTGAAATATTTTTTTGATCTGTTTTA
CGATATTTTAATAGGCTAAA

Product: amidase

Products: ADP; phosphate; Gln-tRNA(Gln); L-glutamate

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MEMQFNTLLQKELTIHDIQTAMEDEKLTSKELVMYYLHRIAKYDQDGPKINSILEINPDAIFIAEALDHERKIKGIRGPL
HGIPVLLKDNIETNDSMHTSAGTIALEQHISSEDAFLVTKLREAGAVILGKTNMTELANAMSFEMWAGYSARGGQTINPY
GTGDDDMFVGGSSTGSAIAVAANFTVVSVGTETDASILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
AAILLGSLTGVDEKDVATHKSEGIGEPDYTKYLDVNGLNGTKIGVYNNAPKEYYETGEYDEKLFKETIEVLHSKGATVVE
DIHIPSFHREWSWGVSLYELKHSLDNYLSKLPSTIPVHSISELMEFNENIAERALKYGQTKLERRKDFPNTLRNPEYLNA
RLEDIYFSQEQGIDFALEKYNLDAILFPSYIGSTICAKAGYPSIAIPAGYMEGGRPFGITLASTAFSEGTLIKLAYAFEQ
ATNHRKIPGLS

Sequences:

>Translated_491_residues
MEMQFNTLLQKELTIHDIQTAMEDEKLTSKELVMYYLHRIAKYDQDGPKINSILEINPDAIFIAEALDHERKIKGIRGPL
HGIPVLLKDNIETNDSMHTSAGTIALEQHISSEDAFLVTKLREAGAVILGKTNMTELANAMSFEMWAGYSARGGQTINPY
GTGDDDMFVGGSSTGSAIAVAANFTVVSVGTETDASILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
AAILLGSLTGVDEKDVATHKSEGIGEPDYTKYLDVNGLNGTKIGVYNNAPKEYYETGEYDEKLFKETIEVLHSKGATVVE
DIHIPSFHREWSWGVSLYELKHSLDNYLSKLPSTIPVHSISELMEFNENIAERALKYGQTKLERRKDFPNTLRNPEYLNA
RLEDIYFSQEQGIDFALEKYNLDAILFPSYIGSTICAKAGYPSIAIPAGYMEGGRPFGITLASTAFSEGTLIKLAYAFEQ
ATNHRKIPGLS
>Mature_491_residues
MEMQFNTLLQKELTIHDIQTAMEDEKLTSKELVMYYLHRIAKYDQDGPKINSILEINPDAIFIAEALDHERKIKGIRGPL
HGIPVLLKDNIETNDSMHTSAGTIALEQHISSEDAFLVTKLREAGAVILGKTNMTELANAMSFEMWAGYSARGGQTINPY
GTGDDDMFVGGSSTGSAIAVAANFTVVSVGTETDASILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
AAILLGSLTGVDEKDVATHKSEGIGEPDYTKYLDVNGLNGTKIGVYNNAPKEYYETGEYDEKLFKETIEVLHSKGATVVE
DIHIPSFHREWSWGVSLYELKHSLDNYLSKLPSTIPVHSISELMEFNENIAERALKYGQTKLERRKDFPNTLRNPEYLNA
RLEDIYFSQEQGIDFALEKYNLDAILFPSYIGSTICAKAGYPSIAIPAGYMEGGRPFGITLASTAFSEGTLIKLAYAFEQ
ATNHRKIPGLS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=533, Percent_Identity=26.6416510318949, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI195972892, Length=253, Percent_Identity=29.6442687747036, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=486, Percent_Identity=24.6913580246914, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17537465, Length=508, Percent_Identity=24.4094488188976, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17556264, Length=262, Percent_Identity=27.8625954198473, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=414, Percent_Identity=27.2946859903382, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323950, Length=209, Percent_Identity=36.8421052631579, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648113, Length=474, Percent_Identity=29.1139240506329, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24648435, Length=256, Percent_Identity=28.515625, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24648437, Length=256, Percent_Identity=28.515625, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI45550774, Length=256, Percent_Identity=28.515625, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21356731, Length=241, Percent_Identity=30.2904564315353, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24644968, Length=519, Percent_Identity=23.3140655105973, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648441, Length=176, Percent_Identity=31.25, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24648439, Length=176, Percent_Identity=31.25, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24652985, Length=498, Percent_Identity=23.0923694779116, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922090, Length=498, Percent_Identity=23.0923694779116, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24652981, Length=498, Percent_Identity=23.0923694779116, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24652983, Length=498, Percent_Identity=23.0923694779116, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.7

Molecular weight: Translated: 54018; Mature: 54018

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMQFNTLLQKELTIHDIQTAMEDEKLTSKELVMYYLHRIAKYDQDGPKINSILEINPDA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCE
IFIAEALDHERKIKGIRGPLHGIPVLLKDNIETNDSMHTSAGTIALEQHISSEDAFLVTK
EEEEHHHCHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCEEHEE
LREAGAVILGKTNMTELANAMSFEMWAGYSARGGQTINPYGTGDDDMFVGGSSTGSAIAV
HHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCEEECCCCCCCEEEE
AANFTVVSVGTETDASILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
EEEEEEEEECCCCCHHHHHHHHHCCCEECCCCHHHHHCCCCCEEEECCCCCCCHHHHHHH
AAILLGSLTGVDEKDVATHKSEGIGEPDYTKYLDVNGLNGTKIGVYNNAPKEYYETGEYD
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEECCCCHHHHHCCCHH
EKLFKETIEVLHSKGATVVEDIHIPSFHREWSWGVSLYELKHSLDNYLSKLPSTIPVHSI
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCHHHH
SELMEFNENIAERALKYGQTKLERRKDFPNTLRNPEYLNARLEDIYFSQEQGIDFALEKY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHEECHHCCCEEEEEHC
NLDAILFPSYIGSTICAKAGYPSIAIPAGYMEGGRPFGITLASTAFSEGTLIKLAYAFEQ
CCCEEEECHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEEECCCCCCCCEEEEEEHHHH
ATNHRKIPGLS
HCCCCCCCCCC
>Mature Secondary Structure
MEMQFNTLLQKELTIHDIQTAMEDEKLTSKELVMYYLHRIAKYDQDGPKINSILEINPDA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCE
IFIAEALDHERKIKGIRGPLHGIPVLLKDNIETNDSMHTSAGTIALEQHISSEDAFLVTK
EEEEHHHCHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCEEHEE
LREAGAVILGKTNMTELANAMSFEMWAGYSARGGQTINPYGTGDDDMFVGGSSTGSAIAV
HHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCEEECCCCCCCEEEE
AANFTVVSVGTETDASILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
EEEEEEEEECCCCCHHHHHHHHHCCCEECCCCHHHHHCCCCCEEEECCCCCCCHHHHHHH
AAILLGSLTGVDEKDVATHKSEGIGEPDYTKYLDVNGLNGTKIGVYNNAPKEYYETGEYD
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEECCCCHHHHHCCCHH
EKLFKETIEVLHSKGATVVEDIHIPSFHREWSWGVSLYELKHSLDNYLSKLPSTIPVHSI
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCHHHH
SELMEFNENIAERALKYGQTKLERRKDFPNTLRNPEYLNARLEDIYFSQEQGIDFALEKY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHEECHHCCCEEEEEHC
NLDAILFPSYIGSTICAKAGYPSIAIPAGYMEGGRPFGITLASTAFSEGTLIKLAYAFEQ
CCCEEEECHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEEECCCCCCCCEEEEEEHHHH
ATNHRKIPGLS
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Glu-tRNA(Gln); L-glutamine

Specific reaction: ATP + Glu-tRNA(Gln) + L-glutamine = ADP + phosphate + Gln-tRNA(Gln) + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA